Amino acid dipepetide frequency for Trichormus variabilis SAG 1403-4b

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	7.084AlaAla: 7.084 ± 0.097	0.752AlaCys: 0.752 ± 0.025	3.995AlaAsp: 3.995 ± 0.054	5.233AlaGlu: 5.233 ± 0.066	2.782AlaPhe: 2.782 ± 0.047	5.255AlaGly: 5.255 ± 0.08	1.187AlaHis: 1.187 ± 0.029	6.525AlaIle: 6.525 ± 0.069	4.262AlaLys: 4.262 ± 0.062	7.843AlaLeu: 7.843 ± 0.084	1.575AlaMet: 1.575 ± 0.04	3.64AlaAsn: 3.64 ± 0.064	2.585AlaPro: 2.585 ± 0.044	3.781AlaGln: 3.781 ± 0.057	3.17AlaArg: 3.17 ± 0.05	4.324AlaSer: 4.324 ± 0.061	4.473AlaThr: 4.473 ± 0.052	5.313AlaVal: 5.313 ± 0.056	0.984AlaTrp: 0.984 ± 0.028	2.279AlaTyr: 2.279 ± 0.052	0.0AlaXaa: 0.0 ± 0.0
Cys	0.61CysAla: 0.61 ± 0.017	0.165CysCys: 0.165 ± 0.011	0.535CysAsp: 0.535 ± 0.02	0.543CysGlu: 0.543 ± 0.019	0.419CysPhe: 0.419 ± 0.018	0.781CysGly: 0.781 ± 0.026	0.277CysHis: 0.277 ± 0.012	0.605CysIle: 0.605 ± 0.02	0.367CysLys: 0.367 ± 0.016	1.135CysLeu: 1.135 ± 0.031	0.157CysMet: 0.157 ± 0.01	0.366CysAsn: 0.366 ± 0.015	0.529CysPro: 0.529 ± 0.019	0.602CysGln: 0.602 ± 0.019	0.469CysArg: 0.469 ± 0.019	0.612CysSer: 0.612 ± 0.02	0.455CysThr: 0.455 ± 0.019	0.566CysVal: 0.566 ± 0.02	0.145CysTrp: 0.145 ± 0.01	0.369CysTyr: 0.369 ± 0.014	0.0CysXaa: 0.0 ± 0.0
Asp	3.53AspAla: 3.53 ± 0.046	0.512AspCys: 0.512 ± 0.017	2.326AspAsp: 2.326 ± 0.053	2.961AspGlu: 2.961 ± 0.046	2.422AspPhe: 2.422 ± 0.047	3.145AspGly: 3.145 ± 0.059	0.762AspHis: 0.762 ± 0.025	3.873AspIle: 3.873 ± 0.059	2.605AspLys: 2.605 ± 0.053	5.587AspLeu: 5.587 ± 0.063	0.811AspMet: 0.811 ± 0.022	2.247AspAsn: 2.247 ± 0.036	2.027AspPro: 2.027 ± 0.042	1.787AspGln: 1.787 ± 0.036	2.508AspArg: 2.508 ± 0.041	2.924AspSer: 2.924 ± 0.043	2.522AspThr: 2.522 ± 0.047	3.04AspVal: 3.04 ± 0.048	0.896AspTrp: 0.896 ± 0.024	1.893AspTyr: 1.893 ± 0.042	0.001AspXaa: 0.001 ± 0.001
Glu	4.918GluAla: 4.918 ± 0.068	0.499GluCys: 0.499 ± 0.018	2.786GluAsp: 2.786 ± 0.046	4.278GluGlu: 4.278 ± 0.058	2.565GluPhe: 2.565 ± 0.043	3.053GluGly: 3.053 ± 0.047	0.983GluHis: 0.983 ± 0.027	5.422GluIle: 5.422 ± 0.079	4.03GluLys: 4.03 ± 0.057	7.25GluLeu: 7.25 ± 0.085	1.381GluMet: 1.381 ± 0.03	3.243GluAsn: 3.243 ± 0.049	2.325GluPro: 2.325 ± 0.041	3.521GluGln: 3.521 ± 0.055	3.201GluArg: 3.201 ± 0.053	3.482GluSer: 3.482 ± 0.053	3.623GluThr: 3.623 ± 0.055	4.343GluVal: 4.343 ± 0.057	0.819GluTrp: 0.819 ± 0.021	2.02GluTyr: 2.02 ± 0.038	0.001GluXaa: 0.001 ± 0.001
Phe	3.002PheAla: 3.002 ± 0.049	0.538PheCys: 0.538 ± 0.017	2.235PheAsp: 2.235 ± 0.038	2.131PheGlu: 2.131 ± 0.044	1.663PhePhe: 1.663 ± 0.036	2.903PheGly: 2.903 ± 0.046	0.773PheHis: 0.773 ± 0.023	2.648PheIle: 2.648 ± 0.046	1.697PheLys: 1.697 ± 0.04	4.21PheLeu: 4.21 ± 0.054	0.693PheMet: 0.693 ± 0.02	1.879PheAsn: 1.879 ± 0.038	1.85PhePro: 1.85 ± 0.036	1.855PheGln: 1.855 ± 0.036	1.697PheArg: 1.697 ± 0.036	3.003PheSer: 3.003 ± 0.045	2.458PheThr: 2.458 ± 0.04	2.387PheVal: 2.387 ± 0.039	0.691PheTrp: 0.691 ± 0.025	1.406PheTyr: 1.406 ± 0.035	0.0PheXaa: 0.0 ± 0.0
Gly	4.524GlyAla: 4.524 ± 0.075	0.764GlyCys: 0.764 ± 0.025	3.182GlyAsp: 3.182 ± 0.068	4.033GlyGlu: 4.033 ± 0.062	2.937GlyPhe: 2.937 ± 0.042	4.61GlyGly: 4.61 ± 0.09	1.129GlyHis: 1.129 ± 0.034	5.261GlyIle: 5.261 ± 0.068	4.345GlyLys: 4.345 ± 0.056	6.785GlyLeu: 6.785 ± 0.081	1.43GlyMet: 1.43 ± 0.029	3.346GlyAsn: 3.346 ± 0.084	1.086GlyPro: 1.086 ± 0.028	2.721GlyGln: 2.721 ± 0.038	2.974GlyArg: 2.974 ± 0.045	3.932GlySer: 3.932 ± 0.065	3.814GlyThr: 3.814 ± 0.061	4.742GlyVal: 4.742 ± 0.072	1.072GlyTrp: 1.072 ± 0.028	2.304GlyTyr: 2.304 ± 0.043	0.001GlyXaa: 0.001 ± 0.001
His	0.98HisAla: 0.98 ± 0.028	0.248HisCys: 0.248 ± 0.011	0.742HisAsp: 0.742 ± 0.021	0.917HisGlu: 0.917 ± 0.024	0.788HisPhe: 0.788 ± 0.02	1.095HisGly: 1.095 ± 0.03	0.604HisHis: 0.604 ± 0.023	1.267HisIle: 1.267 ± 0.028	0.847HisLys: 0.847 ± 0.023	2.221HisLeu: 2.221 ± 0.041	0.214HisMet: 0.214 ± 0.012	0.812HisAsn: 0.812 ± 0.02	1.293HisPro: 1.293 ± 0.029	1.179HisGln: 1.179 ± 0.026	0.997HisArg: 0.997 ± 0.026	1.17HisSer: 1.17 ± 0.027	0.945HisThr: 0.945 ± 0.026	0.761HisVal: 0.761 ± 0.026	0.32HisTrp: 0.32 ± 0.013	0.652HisTyr: 0.652 ± 0.02	0.0HisXaa: 0.0 ± 0.0
Ile	6.809IleAla: 6.809 ± 0.071	0.812IleCys: 0.812 ± 0.027	3.903IleAsp: 3.903 ± 0.052	4.499IleGlu: 4.499 ± 0.056	2.884IlePhe: 2.884 ± 0.049	4.64IleGly: 4.64 ± 0.059	1.353IleHis: 1.353 ± 0.029	4.64IleIle: 4.64 ± 0.061	3.64IleLys: 3.64 ± 0.051	7.287IleLeu: 7.287 ± 0.085	0.992IleMet: 0.992 ± 0.027	3.747IleAsn: 3.747 ± 0.053	3.833IlePro: 3.833 ± 0.055	3.299IleGln: 3.299 ± 0.053	3.164IleArg: 3.164 ± 0.045	5.055IleSer: 5.055 ± 0.064	4.357IleThr: 4.357 ± 0.062	4.34IleVal: 4.34 ± 0.056	0.943IleTrp: 0.943 ± 0.028	2.194IleTyr: 2.194 ± 0.036	0.001IleXaa: 0.001 ± 0.001
Lys	4.119LysAla: 4.119 ± 0.057	0.35LysCys: 0.35 ± 0.016	2.391LysAsp: 2.391 ± 0.041	3.008LysGlu: 3.008 ± 0.052	2.037LysPhe: 2.037 ± 0.043	2.792LysGly: 2.792 ± 0.05	0.832LysHis: 0.832 ± 0.021	4.284LysIle: 4.284 ± 0.053	2.779LysLys: 2.779 ± 0.057	5.884LysLeu: 5.884 ± 0.073	1.058LysMet: 1.058 ± 0.028	2.574LysAsn: 2.574 ± 0.047	2.596LysPro: 2.596 ± 0.048	2.902LysGln: 2.902 ± 0.047	2.425LysArg: 2.425 ± 0.043	3.442LysSer: 3.442 ± 0.051	3.227LysThr: 3.227 ± 0.054	3.328LysVal: 3.328 ± 0.046	0.602LysTrp: 0.602 ± 0.02	1.709LysTyr: 1.709 ± 0.04	0.0LysXaa: 0.0 ± 0.0
Leu	9.156LeuAla: 9.156 ± 0.099	0.985LeuCys: 0.985 ± 0.027	5.194LeuAsp: 5.194 ± 0.06	7.461LeuGlu: 7.461 ± 0.093	3.802LeuPhe: 3.802 ± 0.057	7.566LeuGly: 7.566 ± 0.077	1.845LeuHis: 1.845 ± 0.039	7.158LeuIle: 7.158 ± 0.077	5.801LeuLys: 5.801 ± 0.067	11.186LeuLeu: 11.186 ± 0.101	2.116LeuMet: 2.116 ± 0.039	4.948LeuAsn: 4.948 ± 0.063	5.712LeuPro: 5.712 ± 0.07	5.987LeuGln: 5.987 ± 0.071	5.299LeuArg: 5.299 ± 0.072	7.439LeuSer: 7.439 ± 0.075	6.42LeuThr: 6.42 ± 0.073	7.088LeuVal: 7.088 ± 0.081	1.405LeuTrp: 1.405 ± 0.033	2.692LeuTyr: 2.692 ± 0.049	0.001LeuXaa: 0.001 ± 0.001
Met	1.572MetAla: 1.572 ± 0.031	0.129MetCys: 0.129 ± 0.01	0.743MetAsp: 0.743 ± 0.023	0.989MetGlu: 0.989 ± 0.026	0.581MetPhe: 0.581 ± 0.022	1.35MetGly: 1.35 ± 0.03	0.282MetHis: 0.282 ± 0.013	1.215MetIle: 1.215 ± 0.027	0.978MetLys: 0.978 ± 0.029	1.953MetLeu: 1.953 ± 0.035	0.454MetMet: 0.454 ± 0.019	0.929MetAsn: 0.929 ± 0.021	0.891MetPro: 0.891 ± 0.023	0.955MetGln: 0.955 ± 0.026	0.92MetArg: 0.92 ± 0.023	1.316MetSer: 1.316 ± 0.029	1.312MetThr: 1.312 ± 0.031	1.232MetVal: 1.232 ± 0.03	0.171MetTrp: 0.171 ± 0.011	0.371MetTyr: 0.371 ± 0.016	0.0MetXaa: 0.0 ± 0.0
Asn	3.01AsnAla: 3.01 ± 0.05	0.484AsnCys: 0.484 ± 0.018	1.843AsnAsp: 1.843 ± 0.038	2.081AsnGlu: 2.081 ± 0.038	2.142AsnPhe: 2.142 ± 0.04	2.805AsnGly: 2.805 ± 0.06	0.988AsnHis: 0.988 ± 0.026	3.43AsnIle: 3.43 ± 0.056	2.124AsnLys: 2.124 ± 0.04	5.982AsnLeu: 5.982 ± 0.078	0.704AsnMet: 0.704 ± 0.02	2.537AsnAsn: 2.537 ± 0.06	2.954AsnPro: 2.954 ± 0.047	3.042AsnGln: 3.042 ± 0.048	2.456AsnArg: 2.456 ± 0.043	3.501AsnSer: 3.501 ± 0.056	2.598AsnThr: 2.598 ± 0.05	2.41AsnVal: 2.41 ± 0.041	0.842AsnTrp: 0.842 ± 0.024	1.793AsnTyr: 1.793 ± 0.037	0.0AsnXaa: 0.0 ± 0.0
Pro	3.125ProAla: 3.125 ± 0.051	0.347ProCys: 0.347 ± 0.016	2.784ProAsp: 2.784 ± 0.049	3.878ProGlu: 3.878 ± 0.053	1.709ProPhe: 1.709 ± 0.031	2.939ProGly: 2.939 ± 0.047	0.919ProHis: 0.919 ± 0.028	3.193ProIle: 3.193 ± 0.046	2.275ProLys: 2.275 ± 0.04	4.479ProLeu: 4.479 ± 0.052	0.718ProMet: 0.718 ± 0.02	2.296ProAsn: 2.296 ± 0.043	2.08ProPro: 2.08 ± 0.04	2.625ProGln: 2.625 ± 0.046	1.574ProArg: 1.574 ± 0.031	2.722ProSer: 2.722 ± 0.045	2.921ProThr: 2.921 ± 0.051	3.178ProVal: 3.178 ± 0.047	0.57ProTrp: 0.57 ± 0.017	1.313ProTyr: 1.313 ± 0.029	0.002ProXaa: 0.002 ± 0.001
Gln	4.464GlnAla: 4.464 ± 0.058	0.331GlnCys: 0.331 ± 0.015	2.158GlnAsp: 2.158 ± 0.039	3.948GlnGlu: 3.948 ± 0.062	1.752GlnPhe: 1.752 ± 0.032	3.125GlnGly: 3.125 ± 0.047	0.826GlnHis: 0.826 ± 0.022	3.953GlnIle: 3.953 ± 0.059	3.089GlnLys: 3.089 ± 0.047	6.025GlnLeu: 6.025 ± 0.074	1.078GlnMet: 1.078 ± 0.025	2.368GlnAsn: 2.368 ± 0.04	2.645GlnPro: 2.645 ± 0.042	3.808GlnGln: 3.808 ± 0.072	2.758GlnArg: 2.758 ± 0.048	2.949GlnSer: 2.949 ± 0.049	2.931GlnThr: 2.931 ± 0.044	3.768GlnVal: 3.768 ± 0.052	0.616GlnTrp: 0.616 ± 0.017	1.294GlnTyr: 1.294 ± 0.031	0.0GlnXaa: 0.0 ± 0.0
Arg	2.801ArgAla: 2.801 ± 0.044	0.491ArgCys: 0.491 ± 0.019	2.329ArgAsp: 2.329 ± 0.047	3.196ArgGlu: 3.196 ± 0.048	1.984ArgPhe: 1.984 ± 0.04	2.714ArgGly: 2.714 ± 0.04	0.952ArgHis: 0.952 ± 0.03	3.261ArgIle: 3.261 ± 0.045	2.327ArgLys: 2.327 ± 0.048	5.613ArgLeu: 5.613 ± 0.074	0.923ArgMet: 0.923 ± 0.028	2.168ArgAsn: 2.168 ± 0.038	1.849ArgPro: 1.849 ± 0.037	3.003ArgGln: 3.003 ± 0.054	2.72ArgArg: 2.72 ± 0.05	2.937ArgSer: 2.937 ± 0.046	2.385ArgThr: 2.385 ± 0.037	3.12ArgVal: 3.12 ± 0.05	0.738ArgTrp: 0.738 ± 0.019	1.791ArgTyr: 1.791 ± 0.033	0.001ArgXaa: 0.001 ± 0.001
Ser	4.273SerAla: 4.273 ± 0.057	0.652SerCys: 0.652 ± 0.023	3.066SerAsp: 3.066 ± 0.046	3.851SerGlu: 3.851 ± 0.052	2.519SerPhe: 2.519 ± 0.04	4.533SerGly: 4.533 ± 0.066	1.339SerHis: 1.339 ± 0.03	4.093SerIle: 4.093 ± 0.047	2.845SerLys: 2.845 ± 0.047	7.573SerLeu: 7.573 ± 0.088	1.146SerMet: 1.146 ± 0.026	2.924SerAsn: 2.924 ± 0.043	3.34SerPro: 3.34 ± 0.052	3.763SerGln: 3.763 ± 0.055	3.068SerArg: 3.068 ± 0.045	4.407SerSer: 4.407 ± 0.06	3.583SerThr: 3.583 ± 0.058	4.004SerVal: 4.004 ± 0.059	0.952SerTrp: 0.952 ± 0.023	1.89SerTyr: 1.89 ± 0.034	0.001SerXaa: 0.001 ± 0.001
Thr	4.832ThrAla: 4.832 ± 0.054	0.457ThrCys: 0.457 ± 0.017	2.725ThrAsp: 2.725 ± 0.043	3.558ThrGlu: 3.558 ± 0.047	2.178ThrPhe: 2.178 ± 0.041	4.326ThrGly: 4.326 ± 0.062	1.017ThrHis: 1.017 ± 0.026	3.92ThrIle: 3.92 ± 0.055	2.609ThrLys: 2.609 ± 0.04	6.247ThrLeu: 6.247 ± 0.068	0.771ThrMet: 0.771 ± 0.024	2.576ThrAsn: 2.576 ± 0.046	3.425ThrPro: 3.425 ± 0.055	2.921ThrGln: 2.921 ± 0.047	2.303ThrArg: 2.303 ± 0.037	3.601ThrSer: 3.601 ± 0.059	3.592ThrThr: 3.592 ± 0.055	4.086ThrVal: 4.086 ± 0.061	0.714ThrTrp: 0.714 ± 0.022	1.67ThrTyr: 1.67 ± 0.032	0.001ThrXaa: 0.001 ± 0.001
Val	5.394ValAla: 5.394 ± 0.069	0.645ValCys: 0.645 ± 0.019	3.346ValAsp: 3.346 ± 0.051	4.448ValGlu: 4.448 ± 0.05	2.587ValPhe: 2.587 ± 0.044	4.401ValGly: 4.401 ± 0.061	0.973ValHis: 0.973 ± 0.024	4.785ValIle: 4.785 ± 0.056	3.602ValLys: 3.602 ± 0.049	6.368ValLeu: 6.368 ± 0.069	1.368ValMet: 1.368 ± 0.029	3.12ValAsn: 3.12 ± 0.048	2.731ValPro: 2.731 ± 0.044	2.82ValGln: 2.82 ± 0.047	2.96ValArg: 2.96 ± 0.053	4.223ValSer: 4.223 ± 0.058	3.733ValThr: 3.733 ± 0.058	4.645ValVal: 4.645 ± 0.059	0.829ValTrp: 0.829 ± 0.026	1.889ValTyr: 1.889 ± 0.034	0.0ValXaa: 0.0 ± 0.0
Trp	0.769TrpAla: 0.769 ± 0.024	0.148TrpCys: 0.148 ± 0.01	0.639TrpAsp: 0.639 ± 0.024	0.992TrpGlu: 0.992 ± 0.025	0.614TrpPhe: 0.614 ± 0.022	0.965TrpGly: 0.965 ± 0.024	0.311TrpHis: 0.311 ± 0.014	0.889TrpIle: 0.889 ± 0.025	0.691TrpLys: 0.691 ± 0.023	1.89TrpLeu: 1.89 ± 0.038	0.308TrpMet: 0.308 ± 0.017	0.658TrpAsn: 0.658 ± 0.023	0.279TrpPro: 0.279 ± 0.012	1.194TrpGln: 1.194 ± 0.028	0.783TrpArg: 0.783 ± 0.022	0.773TrpSer: 0.773 ± 0.024	0.612TrpThr: 0.612 ± 0.024	0.91TrpVal: 0.91 ± 0.024	0.24TrpTrp: 0.24 ± 0.013	0.414TrpTyr: 0.414 ± 0.017	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.068TyrAla: 2.068 ± 0.037	0.4TyrCys: 0.4 ± 0.018	1.458TyrAsp: 1.458 ± 0.032	1.738TyrGlu: 1.738 ± 0.034	1.355TyrPhe: 1.355 ± 0.029	1.993TyrGly: 1.993 ± 0.035	0.701TyrHis: 0.701 ± 0.022	1.97TyrIle: 1.97 ± 0.038	1.427TyrLys: 1.427 ± 0.035	3.74TyrLeu: 3.74 ± 0.058	0.445TyrMet: 0.445 ± 0.016	1.347TyrAsn: 1.347 ± 0.033	1.615TyrPro: 1.615 ± 0.032	2.117TyrGln: 2.117 ± 0.041	1.857TyrArg: 1.857 ± 0.03	2.012TyrSer: 2.012 ± 0.039	1.567TyrThr: 1.567 ± 0.031	1.603TyrVal: 1.603 ± 0.035	0.529TyrTrp: 0.529 ± 0.017	1.147TyrTyr: 1.147 ± 0.029	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.001XaaAla: 0.001 ± 0.001	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.001XaaGlu: 0.001 ± 0.0	0.001XaaPhe: 0.001 ± 0.001	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.001XaaIle: 0.001 ± 0.001	0.0XaaLys: 0.0 ± 0.0	0.001XaaLeu: 0.001 ± 0.001	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.001XaaPro: 0.001 ± 0.001	0.001XaaGln: 0.001 ± 0.001	0.001XaaArg: 0.001 ± 0.001	0.001XaaSer: 0.001 ± 0.001	0.001XaaThr: 0.001 ± 0.001	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.001XaaTyr: 0.001 ± 0.001	0.075XaaXaa: 0.075 ± 0.039

Statistics based on 5168 proteins (1610029 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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