Sporolactobacillus sp. THM7-7

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Sporolactobacillaceae; Sporolactobacillus; unclassified Sporolactobacillus

Average proteome isoelectric point is 7.03

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2659 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4Q4J666|A0A4Q4J666_9BACL PTS beta-glucoside transporter subunit EIIBCA OS=Sporolactobacillus sp. THM7-7 OX=2511172 GN=EWH99_12095 PE=4 SV=1
MM1 pKa = 7.25NLKK4 pKa = 10.58DD5 pKa = 3.72YY6 pKa = 11.33VIVDD10 pKa = 3.75KK11 pKa = 8.19EE12 pKa = 4.35TCIACGACGCSAPDD26 pKa = 3.24VFAYY30 pKa = 10.53DD31 pKa = 3.31EE32 pKa = 5.25DD33 pKa = 4.06GTSRR37 pKa = 11.84VTLDD41 pKa = 4.16DD42 pKa = 3.86NQGTAAIPDD51 pKa = 4.23DD52 pKa = 4.8LLDD55 pKa = 4.37DD56 pKa = 5.26VIDD59 pKa = 4.74AYY61 pKa = 10.23EE62 pKa = 4.41GCPTEE67 pKa = 4.36SIKK70 pKa = 10.24MSKK73 pKa = 8.83TPFNGNPAKK82 pKa = 10.45EE83 pKa = 4.01EE84 pKa = 4.34SEE86 pKa = 4.23ASS88 pKa = 3.35

Molecular weight: 9.36 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4Q4JEB3|A0A4Q4JEB3_9BACL Dihydropteroate pyrophosphorylase OS=Sporolactobacillus sp. THM7-7 OX=2511172 GN=folP PE=3 SV=1
MM1 pKa = 7.44KK2 pKa = 9.56RR3 pKa = 11.84TFQPNTRR10 pKa = 11.84KK11 pKa = 9.78RR12 pKa = 11.84KK13 pKa = 8.49KK14 pKa = 8.81VHH16 pKa = 5.47GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.37NGRR28 pKa = 11.84KK29 pKa = 9.17VLANRR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84KK37 pKa = 8.69GRR39 pKa = 11.84KK40 pKa = 8.69VLSAA44 pKa = 4.05

Molecular weight: 5.24 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2659

0

2659

812802

18

1518

305.7

34.16

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.026 ± 0.051

0.86 ± 0.017

5.358 ± 0.042

6.506 ± 0.054

4.344 ± 0.042

7.396 ± 0.048

2.257 ± 0.022

7.044 ± 0.039

6.382 ± 0.049

9.672 ± 0.048

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.73 ± 0.021

3.66 ± 0.03

4.004 ± 0.029

3.504 ± 0.028

5.649 ± 0.043

5.96 ± 0.038

5.297 ± 0.035

7.052 ± 0.036

1.018 ± 0.02

3.28 ± 0.028
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski