Amino acid dipepetide frequency for Sporolactobacillus sp. THM7-7

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	8.013AlaAla: 8.013 ± 0.129	0.803AlaCys: 0.803 ± 0.037	4.477AlaAsp: 4.477 ± 0.079	5.444AlaGlu: 5.444 ± 0.1	3.78AlaPhe: 3.78 ± 0.078	6.508AlaGly: 6.508 ± 0.106	1.531AlaHis: 1.531 ± 0.043	5.684AlaIle: 5.684 ± 0.081	4.462AlaLys: 4.462 ± 0.079	8.244AlaLeu: 8.244 ± 0.111	2.159AlaMet: 2.159 ± 0.051	2.454AlaAsn: 2.454 ± 0.062	2.405AlaPro: 2.405 ± 0.057	2.345AlaGln: 2.345 ± 0.058	4.145AlaArg: 4.145 ± 0.073	4.681AlaSer: 4.681 ± 0.084	3.354AlaThr: 3.354 ± 0.069	6.446AlaVal: 6.446 ± 0.104	0.699AlaTrp: 0.699 ± 0.026	2.629AlaTyr: 2.629 ± 0.056	0.0AlaXaa: 0.0 ± 0.0
Cys	0.636CysAla: 0.636 ± 0.028	0.125CysCys: 0.125 ± 0.015	0.466CysAsp: 0.466 ± 0.028	0.48CysGlu: 0.48 ± 0.035	0.379CysPhe: 0.379 ± 0.019	0.844CysGly: 0.844 ± 0.033	0.23CysHis: 0.23 ± 0.017	0.543CysIle: 0.543 ± 0.026	0.31CysLys: 0.31 ± 0.02	0.931CysLeu: 0.931 ± 0.032	0.234CysMet: 0.234 ± 0.017	0.223CysAsn: 0.223 ± 0.016	0.486CysPro: 0.486 ± 0.028	0.274CysGln: 0.274 ± 0.023	0.595CysArg: 0.595 ± 0.028	0.562CysSer: 0.562 ± 0.026	0.458CysThr: 0.458 ± 0.024	0.485CysVal: 0.485 ± 0.024	0.082CysTrp: 0.082 ± 0.01	0.261CysTyr: 0.261 ± 0.019	0.0CysXaa: 0.0 ± 0.0
Asp	4.0AspAla: 4.0 ± 0.077	0.437AspCys: 0.437 ± 0.026	2.655AspAsp: 2.655 ± 0.064	3.953AspGlu: 3.953 ± 0.086	2.384AspPhe: 2.384 ± 0.054	3.84AspGly: 3.84 ± 0.074	1.336AspHis: 1.336 ± 0.046	3.792AspIle: 3.792 ± 0.067	2.943AspLys: 2.943 ± 0.059	5.199AspLeu: 5.199 ± 0.088	1.359AspMet: 1.359 ± 0.038	1.475AspAsn: 1.475 ± 0.046	2.573AspPro: 2.573 ± 0.056	2.184AspGln: 2.184 ± 0.054	3.61AspArg: 3.61 ± 0.068	2.517AspSer: 2.517 ± 0.053	2.493AspThr: 2.493 ± 0.057	3.98AspVal: 3.98 ± 0.077	0.68AspTrp: 0.68 ± 0.031	2.169AspTyr: 2.169 ± 0.053	0.0AspXaa: 0.0 ± 0.0
Glu	5.984GluAla: 5.984 ± 0.102	0.543GluCys: 0.543 ± 0.06	3.095GluAsp: 3.095 ± 0.066	5.146GluGlu: 5.146 ± 0.078	1.617GluPhe: 1.617 ± 0.042	3.866GluGly: 3.866 ± 0.066	1.43GluHis: 1.43 ± 0.048	4.098GluIle: 4.098 ± 0.077	6.299GluLys: 6.299 ± 0.103	5.556GluLeu: 5.556 ± 0.088	1.981GluMet: 1.981 ± 0.052	2.94GluAsn: 2.94 ± 0.07	2.09GluPro: 2.09 ± 0.053	2.666GluGln: 2.666 ± 0.073	4.263GluArg: 4.263 ± 0.077	3.521GluSer: 3.521 ± 0.085	3.925GluThr: 3.925 ± 0.069	3.729GluVal: 3.729 ± 0.068	0.785GluTrp: 0.785 ± 0.036	1.528GluTyr: 1.528 ± 0.048	0.0GluXaa: 0.0 ± 0.0
Phe	3.222PheAla: 3.222 ± 0.076	0.459PheCys: 0.459 ± 0.024	2.496PheAsp: 2.496 ± 0.061	2.137PheGlu: 2.137 ± 0.049	2.456PhePhe: 2.456 ± 0.072	3.45PheGly: 3.45 ± 0.076	1.112PheHis: 1.112 ± 0.037	3.204PheIle: 3.204 ± 0.072	2.062PheLys: 2.062 ± 0.055	4.343PheLeu: 4.343 ± 0.083	1.043PheMet: 1.043 ± 0.038	1.549PheAsn: 1.549 ± 0.044	1.793PhePro: 1.793 ± 0.049	1.548PheGln: 1.548 ± 0.047	2.095PheArg: 2.095 ± 0.056	3.259PheSer: 3.259 ± 0.069	2.309PheThr: 2.309 ± 0.058	2.945PheVal: 2.945 ± 0.058	0.431PheTrp: 0.431 ± 0.022	1.528PheTyr: 1.528 ± 0.046	0.0PheXaa: 0.0 ± 0.0
Gly	5.712GlyAla: 5.712 ± 0.101	0.734GlyCys: 0.734 ± 0.032	3.356GlyAsp: 3.356 ± 0.067	4.299GlyGlu: 4.299 ± 0.074	3.403GlyPhe: 3.403 ± 0.064	5.625GlyGly: 5.625 ± 0.101	1.684GlyHis: 1.684 ± 0.052	5.605GlyIle: 5.605 ± 0.099	5.292GlyLys: 5.292 ± 0.081	7.148GlyLeu: 7.148 ± 0.092	2.285GlyMet: 2.285 ± 0.051	2.315GlyAsn: 2.315 ± 0.06	2.073GlyPro: 2.073 ± 0.054	2.473GlyGln: 2.473 ± 0.056	4.211GlyArg: 4.211 ± 0.079	4.425GlySer: 4.425 ± 0.084	4.358GlyThr: 4.358 ± 0.098	5.205GlyVal: 5.205 ± 0.081	0.878GlyTrp: 0.878 ± 0.04	2.88GlyTyr: 2.88 ± 0.055	0.0GlyXaa: 0.0 ± 0.0
His	1.604HisAla: 1.604 ± 0.048	0.257HisCys: 0.257 ± 0.018	1.168HisAsp: 1.168 ± 0.038	1.362HisGlu: 1.362 ± 0.043	1.202HisPhe: 1.202 ± 0.043	1.581HisGly: 1.581 ± 0.051	0.706HisHis: 0.706 ± 0.032	1.337HisIle: 1.337 ± 0.042	0.918HisLys: 0.918 ± 0.039	2.466HisLeu: 2.466 ± 0.06	0.594HisMet: 0.594 ± 0.027	0.587HisAsn: 0.587 ± 0.028	1.49HisPro: 1.49 ± 0.046	0.845HisGln: 0.845 ± 0.036	1.31HisArg: 1.31 ± 0.038	1.217HisSer: 1.217 ± 0.046	1.004HisThr: 1.004 ± 0.036	1.705HisVal: 1.705 ± 0.043	0.268HisTrp: 0.268 ± 0.019	0.947HisTyr: 0.947 ± 0.039	0.0HisXaa: 0.0 ± 0.0
Ile	5.968IleAla: 5.968 ± 0.103	0.672IleCys: 0.672 ± 0.031	4.412IleAsp: 4.412 ± 0.084	4.387IleGlu: 4.387 ± 0.074	2.79IlePhe: 2.79 ± 0.076	6.192IleGly: 6.192 ± 0.105	1.765IleHis: 1.765 ± 0.046	4.548IleIle: 4.548 ± 0.089	3.809IleLys: 3.809 ± 0.084	6.363IleLeu: 6.363 ± 0.09	1.609IleMet: 1.609 ± 0.048	2.47IleAsn: 2.47 ± 0.063	3.131IlePro: 3.131 ± 0.068	2.496IleGln: 2.496 ± 0.058	4.216IleArg: 4.216 ± 0.072	4.096IleSer: 4.096 ± 0.072	3.513IleThr: 3.513 ± 0.07	5.241IleVal: 5.241 ± 0.094	0.613IleTrp: 0.613 ± 0.029	2.144IleTyr: 2.144 ± 0.061	0.0IleXaa: 0.0 ± 0.0
Lys	4.994LysAla: 4.994 ± 0.092	0.273LysCys: 0.273 ± 0.019	3.257LysAsp: 3.257 ± 0.063	5.234LysGlu: 5.234 ± 0.08	1.471LysPhe: 1.471 ± 0.04	4.213LysGly: 4.213 ± 0.076	1.196LysHis: 1.196 ± 0.039	4.393LysIle: 4.393 ± 0.079	6.133LysLys: 6.133 ± 0.089	4.952LysLeu: 4.952 ± 0.091	2.1LysMet: 2.1 ± 0.049	2.939LysAsn: 2.939 ± 0.057	2.359LysPro: 2.359 ± 0.066	2.534LysGln: 2.534 ± 0.061	3.884LysArg: 3.884 ± 0.066	3.193LysSer: 3.193 ± 0.068	3.695LysThr: 3.695 ± 0.067	4.307LysVal: 4.307 ± 0.083	0.738LysTrp: 0.738 ± 0.029	1.957LysTyr: 1.957 ± 0.051	0.0LysXaa: 0.0 ± 0.0
Leu	8.087LeuAla: 8.087 ± 0.116	0.8LeuCys: 0.8 ± 0.03	5.188LeuAsp: 5.188 ± 0.081	5.365LeuGlu: 5.365 ± 0.092	4.825LeuPhe: 4.825 ± 0.097	6.506LeuGly: 6.506 ± 0.099	1.897LeuHis: 1.897 ± 0.051	7.253LeuIle: 7.253 ± 0.109	6.436LeuLys: 6.436 ± 0.092	9.377LeuLeu: 9.377 ± 0.14	2.507LeuMet: 2.507 ± 0.056	4.044LeuAsn: 4.044 ± 0.068	4.257LeuPro: 4.257 ± 0.073	2.939LeuGln: 2.939 ± 0.063	4.615LeuArg: 4.615 ± 0.084	7.24LeuSer: 7.24 ± 0.111	5.629LeuThr: 5.629 ± 0.086	6.086LeuVal: 6.086 ± 0.097	0.797LeuTrp: 0.797 ± 0.034	2.867LeuTyr: 2.867 ± 0.061	0.0LeuXaa: 0.0 ± 0.0
Met	2.367MetAla: 2.367 ± 0.052	0.185MetCys: 0.185 ± 0.015	1.593MetAsp: 1.593 ± 0.048	1.633MetGlu: 1.633 ± 0.039	0.936MetPhe: 0.936 ± 0.039	1.825MetGly: 1.825 ± 0.058	0.525MetHis: 0.525 ± 0.024	2.275MetIle: 2.275 ± 0.048	2.109MetLys: 2.109 ± 0.052	2.345MetLeu: 2.345 ± 0.056	0.851MetMet: 0.851 ± 0.036	1.459MetAsn: 1.459 ± 0.048	1.141MetPro: 1.141 ± 0.037	0.795MetGln: 0.795 ± 0.031	1.419MetArg: 1.419 ± 0.039	1.58MetSer: 1.58 ± 0.039	1.836MetThr: 1.836 ± 0.054	1.692MetVal: 1.692 ± 0.046	0.149MetTrp: 0.149 ± 0.014	0.589MetTyr: 0.589 ± 0.026	0.0MetXaa: 0.0 ± 0.0
Asn	2.672AsnAla: 2.672 ± 0.062	0.258AsnCys: 0.258 ± 0.018	1.889AsnAsp: 1.889 ± 0.043	2.52AsnGlu: 2.52 ± 0.067	1.275AsnPhe: 1.275 ± 0.042	3.114AsnGly: 3.114 ± 0.073	1.047AsnHis: 1.047 ± 0.034	2.582AsnIle: 2.582 ± 0.064	2.122AsnLys: 2.122 ± 0.056	3.093AsnLeu: 3.093 ± 0.057	0.92AsnMet: 0.92 ± 0.034	1.281AsnAsn: 1.281 ± 0.044	1.895AsnPro: 1.895 ± 0.057	1.772AsnGln: 1.772 ± 0.052	2.539AsnArg: 2.539 ± 0.052	1.526AsnSer: 1.526 ± 0.042	1.774AsnThr: 1.774 ± 0.049	2.678AsnVal: 2.678 ± 0.062	0.424AsnTrp: 0.424 ± 0.024	1.222AsnTyr: 1.222 ± 0.041	0.0AsnXaa: 0.0 ± 0.0
Pro	3.007ProAla: 3.007 ± 0.067	0.303ProCys: 0.303 ± 0.018	2.73ProAsp: 2.73 ± 0.055	3.527ProGlu: 3.527 ± 0.078	2.057ProPhe: 2.057 ± 0.048	2.991ProGly: 2.991 ± 0.062	0.856ProHis: 0.856 ± 0.031	2.792ProIle: 2.792 ± 0.059	2.276ProLys: 2.276 ± 0.051	3.794ProLeu: 3.794 ± 0.072	0.89ProMet: 0.89 ± 0.036	1.542ProAsn: 1.542 ± 0.047	1.196ProPro: 1.196 ± 0.043	1.081ProGln: 1.081 ± 0.041	1.496ProArg: 1.496 ± 0.045	2.547ProSer: 2.547 ± 0.062	1.735ProThr: 1.735 ± 0.047	3.328ProVal: 3.328 ± 0.071	0.423ProTrp: 0.423 ± 0.024	1.468ProTyr: 1.468 ± 0.044	0.0ProXaa: 0.0 ± 0.0
Gln	3.178GlnAla: 3.178 ± 0.069	0.199GlnCys: 0.199 ± 0.016	1.51GlnAsp: 1.51 ± 0.046	2.142GlnGlu: 2.142 ± 0.055	1.403GlnPhe: 1.403 ± 0.04	1.955GlnGly: 1.955 ± 0.055	0.655GlnHis: 0.655 ± 0.03	2.415GlnIle: 2.415 ± 0.054	2.609GlnLys: 2.609 ± 0.064	3.636GlnLeu: 3.636 ± 0.078	1.217GlnMet: 1.217 ± 0.039	1.575GlnAsn: 1.575 ± 0.067	1.32GlnPro: 1.32 ± 0.045	1.293GlnGln: 1.293 ± 0.053	1.702GlnArg: 1.702 ± 0.045	2.263GlnSer: 2.263 ± 0.057	2.185GlnThr: 2.185 ± 0.055	2.299GlnVal: 2.299 ± 0.059	0.392GlnTrp: 0.392 ± 0.025	1.094GlnTyr: 1.094 ± 0.041	0.0GlnXaa: 0.0 ± 0.0
Arg	3.764ArgAla: 3.764 ± 0.066	0.418ArgCys: 0.418 ± 0.019	2.724ArgAsp: 2.724 ± 0.059	4.023ArgGlu: 4.023 ± 0.076	2.578ArgPhe: 2.578 ± 0.05	3.211ArgGly: 3.211 ± 0.062	1.426ArgHis: 1.426 ± 0.041	3.83ArgIle: 3.83 ± 0.078	3.957ArgLys: 3.957 ± 0.059	6.257ArgLeu: 6.257 ± 0.093	1.679ArgMet: 1.679 ± 0.048	1.889ArgAsn: 1.889 ± 0.05	2.184ArgPro: 2.184 ± 0.049	2.482ArgGln: 2.482 ± 0.062	3.797ArgArg: 3.797 ± 0.082	3.168ArgSer: 3.168 ± 0.057	2.998ArgThr: 2.998 ± 0.069	3.547ArgVal: 3.547 ± 0.061	0.577ArgTrp: 0.577 ± 0.024	1.981ArgTyr: 1.981 ± 0.05	0.0ArgXaa: 0.0 ± 0.0
Ser	4.521SerAla: 4.521 ± 0.081	0.529SerCys: 0.529 ± 0.028	3.387SerAsp: 3.387 ± 0.072	4.085SerGlu: 4.085 ± 0.075	2.864SerPhe: 2.864 ± 0.067	5.557SerGly: 5.557 ± 0.086	1.298SerHis: 1.298 ± 0.044	4.06SerIle: 4.06 ± 0.072	3.065SerLys: 3.065 ± 0.066	6.054SerLeu: 6.054 ± 0.096	1.596SerMet: 1.596 ± 0.045	1.81SerAsn: 1.81 ± 0.048	2.312SerPro: 2.312 ± 0.054	1.841SerGln: 1.841 ± 0.059	3.329SerArg: 3.329 ± 0.073	3.617SerSer: 3.617 ± 0.086	2.643SerThr: 2.643 ± 0.058	4.577SerVal: 4.577 ± 0.074	0.619SerTrp: 0.619 ± 0.027	1.836SerTyr: 1.836 ± 0.044	0.0SerXaa: 0.0 ± 0.0
Thr	4.54ThrAla: 4.54 ± 0.082	0.432ThrCys: 0.432 ± 0.022	3.035ThrAsp: 3.035 ± 0.059	3.147ThrGlu: 3.147 ± 0.063	2.595ThrPhe: 2.595 ± 0.052	4.642ThrGly: 4.642 ± 0.079	1.116ThrHis: 1.116 ± 0.037	3.8ThrIle: 3.8 ± 0.077	2.725ThrLys: 2.725 ± 0.06	5.391ThrLeu: 5.391 ± 0.097	1.315ThrMet: 1.315 ± 0.037	1.817ThrAsn: 1.817 ± 0.047	2.318ThrPro: 2.318 ± 0.054	1.35ThrGln: 1.35 ± 0.043	2.405ThrArg: 2.405 ± 0.047	2.881ThrSer: 2.881 ± 0.064	2.59ThrThr: 2.59 ± 0.064	4.557ThrVal: 4.557 ± 0.081	0.506ThrTrp: 0.506 ± 0.027	1.812ThrTyr: 1.812 ± 0.043	0.0ThrXaa: 0.0 ± 0.0
Val	5.074ValAla: 5.074 ± 0.087	0.779ValCys: 0.779 ± 0.029	3.748ValAsp: 3.748 ± 0.06	3.723ValGlu: 3.723 ± 0.086	3.249ValPhe: 3.249 ± 0.072	4.811ValGly: 4.811 ± 0.092	1.606ValHis: 1.606 ± 0.045	5.454ValIle: 5.454 ± 0.078	4.112ValLys: 4.112 ± 0.087	6.887ValLeu: 6.887 ± 0.098	1.813ValMet: 1.813 ± 0.045	2.653ValAsn: 2.653 ± 0.073	3.222ValPro: 3.222 ± 0.057	2.317ValGln: 2.317 ± 0.057	4.047ValArg: 4.047 ± 0.069	4.879ValSer: 4.879 ± 0.078	4.245ValThr: 4.245 ± 0.079	4.943ValVal: 4.943 ± 0.1	0.661ValTrp: 0.661 ± 0.029	2.296ValTyr: 2.296 ± 0.054	0.0ValXaa: 0.0 ± 0.0
Trp	0.678TrpAla: 0.678 ± 0.03	0.084TrpCys: 0.084 ± 0.01	0.507TrpAsp: 0.507 ± 0.024	0.524TrpGlu: 0.524 ± 0.026	0.512TrpPhe: 0.512 ± 0.029	0.631TrpGly: 0.631 ± 0.03	0.236TrpHis: 0.236 ± 0.017	0.805TrpIle: 0.805 ± 0.032	0.625TrpLys: 0.625 ± 0.033	1.209TrpLeu: 1.209 ± 0.045	0.36TrpMet: 0.36 ± 0.021	0.48TrpAsn: 0.48 ± 0.024	0.324TrpPro: 0.324 ± 0.021	0.407TrpGln: 0.407 ± 0.024	0.57TrpArg: 0.57 ± 0.029	0.605TrpSer: 0.605 ± 0.03	0.556TrpThr: 0.556 ± 0.028	0.607TrpVal: 0.607 ± 0.027	0.118TrpTrp: 0.118 ± 0.012	0.341TrpTyr: 0.341 ± 0.024	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.243TyrAla: 2.243 ± 0.049	0.315TyrCys: 0.315 ± 0.021	1.886TyrAsp: 1.886 ± 0.05	1.93TyrGlu: 1.93 ± 0.057	1.666TyrPhe: 1.666 ± 0.048	2.603TyrGly: 2.603 ± 0.056	0.917TyrHis: 0.917 ± 0.035	1.967TyrIle: 1.967 ± 0.057	1.559TyrLys: 1.559 ± 0.047	3.468TyrLeu: 3.468 ± 0.07	0.787TyrMet: 0.787 ± 0.031	1.101TyrAsn: 1.101 ± 0.041	1.471TyrPro: 1.471 ± 0.046	1.399TyrGln: 1.399 ± 0.041	2.242TyrArg: 2.242 ± 0.063	1.821TyrSer: 1.821 ± 0.054	1.678TyrThr: 1.678 ± 0.053	2.158TyrVal: 2.158 ± 0.047	0.337TyrTrp: 0.337 ± 0.018	1.249TyrTyr: 1.249 ± 0.043	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 2659 proteins (812802 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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