Aeromonas phage 4_L372XY

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; Plateaulakevirus; Aeromonas virus 4L372XY

Average proteome isoelectric point is 6.16

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 221 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5B9N5N6|A0A5B9N5N6_9CAUD Baseplate_J domain-containing protein OS=Aeromonas phage 4_L372XY OX=2588520 GN=4L372XY_084 PE=4 SV=1
MM1 pKa = 7.36KK2 pKa = 9.81VSKK5 pKa = 9.74MIKK8 pKa = 9.87LLQQMPQDD16 pKa = 3.84LEE18 pKa = 4.46VYY20 pKa = 9.63SYY22 pKa = 10.8CDD24 pKa = 2.93HH25 pKa = 6.4GQTPEE30 pKa = 4.17SSCAPQVVVVVKK42 pKa = 10.46SEE44 pKa = 3.98YY45 pKa = 10.6DD46 pKa = 2.91RR47 pKa = 11.84GSIDD51 pKa = 4.13GYY53 pKa = 8.25MEE55 pKa = 4.83KK56 pKa = 10.61DD57 pKa = 3.35DD58 pKa = 6.1AIDD61 pKa = 3.31SGYY64 pKa = 10.88EE65 pKa = 3.8EE66 pKa = 4.8EE67 pKa = 4.9DD68 pKa = 3.28LVEE71 pKa = 4.74IVLLL75 pKa = 4.18

Molecular weight: 8.49 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5B9N9M1|A0A5B9N9M1_9CAUD Uncharacterized protein OS=Aeromonas phage 4_L372XY OX=2588520 GN=4L372XY_005 PE=4 SV=1
MM1 pKa = 7.87RR2 pKa = 11.84YY3 pKa = 10.67DD4 pKa = 3.43NTFLKK9 pKa = 10.7LLWLLPLFLQPRR21 pKa = 11.84FIRR24 pKa = 11.84VMYY27 pKa = 10.05AKK29 pKa = 10.44YY30 pKa = 9.14WFDD33 pKa = 3.04WFIVYY38 pKa = 10.0QNNFEE43 pKa = 4.24RR44 pKa = 11.84RR45 pKa = 11.84YY46 pKa = 10.13KK47 pKa = 10.1YY48 pKa = 10.4IKK50 pKa = 10.71ALVEE54 pKa = 3.77WEE56 pKa = 3.88EE57 pKa = 3.7FLNIKK62 pKa = 9.97RR63 pKa = 11.84RR64 pKa = 11.84SKK66 pKa = 11.05

Molecular weight: 8.65 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

221

0

221

36605

30

757

165.6

19.01

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.942 ± 0.239

1.768 ± 0.128

6.289 ± 0.151

7.589 ± 0.185

4.592 ± 0.133

5.991 ± 0.184

1.86 ± 0.082

6.685 ± 0.138

8.747 ± 0.233

7.942 ± 0.139

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.541 ± 0.104

5.707 ± 0.141

2.888 ± 0.103

3.991 ± 0.107

3.844 ± 0.129

6.384 ± 0.155

5.349 ± 0.148

6.69 ± 0.154

1.472 ± 0.081

4.726 ± 0.133
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski