Enterococcus avium ATCC 14025

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae; Enterococcus; Enterococcus avium

Average proteome isoelectric point is 6.18

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4469 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|S0RFK7|S0RFK7_ENTAV Uncharacterized protein OS=Enterococcus avium ATCC 14025 OX=1140002 GN=OMU_04574 PE=4 SV=1
MM1 pKa = 7.46GGAFIYY7 pKa = 10.55LSNTEE12 pKa = 4.07NSPANSQTTDD22 pKa = 2.59SSTAEE27 pKa = 3.8ILMADD32 pKa = 3.9TDD34 pKa = 4.14LSTNQLLDD42 pKa = 3.46VPLEE46 pKa = 4.02DD47 pKa = 3.55QYY49 pKa = 11.99GGEE52 pKa = 4.03VAMEE56 pKa = 4.18NGCEE60 pKa = 3.73ITALSMLLNYY70 pKa = 10.0YY71 pKa = 10.71GYY73 pKa = 8.86GTDD76 pKa = 3.56KK77 pKa = 11.61NEE79 pKa = 4.85LVDD82 pKa = 5.39LLDD85 pKa = 4.22FVPLYY90 pKa = 10.79EE91 pKa = 4.55DD92 pKa = 3.42AEE94 pKa = 4.34KK95 pKa = 10.49RR96 pKa = 11.84IHH98 pKa = 6.75GNPHH102 pKa = 6.72DD103 pKa = 4.2GFVGNIYY110 pKa = 10.38EE111 pKa = 4.67GYY113 pKa = 9.18EE114 pKa = 3.9AMGVAVEE121 pKa = 5.21PIAEE125 pKa = 4.24VANEE129 pKa = 3.98VVQDD133 pKa = 3.87NQKK136 pKa = 10.01VVANNDD142 pKa = 2.95TSFDD146 pKa = 3.7DD147 pKa = 4.01LVRR150 pKa = 11.84VIQTGTPVWVITTVDD165 pKa = 4.02FQVPTEE171 pKa = 4.12DD172 pKa = 6.16DD173 pKa = 3.37FRR175 pKa = 11.84TWQTTSGEE183 pKa = 4.24IEE185 pKa = 4.31VSPLCHH191 pKa = 6.15AVIITGVDD199 pKa = 3.37EE200 pKa = 4.48EE201 pKa = 4.4NVYY204 pKa = 11.18VNDD207 pKa = 3.87PYY209 pKa = 11.19GYY211 pKa = 10.2KK212 pKa = 10.36NRR214 pKa = 11.84VVDD217 pKa = 3.54RR218 pKa = 11.84TDD220 pKa = 3.38FEE222 pKa = 4.95EE223 pKa = 5.3IYY225 pKa = 10.88QLMGSEE231 pKa = 4.26SLYY234 pKa = 11.31LEE236 pKa = 5.13DD237 pKa = 5.14KK238 pKa = 11.1

Molecular weight: 26.46 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|S0S7Q4|S0S7Q4_ENTAV OmpR/PhoB-type domain-containing protein OS=Enterococcus avium ATCC 14025 OX=1140002 GN=OMU_00736 PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 9.42RR3 pKa = 11.84TYY5 pKa = 10.34QPNKK9 pKa = 9.16RR10 pKa = 11.84KK11 pKa = 9.12RR12 pKa = 11.84QKK14 pKa = 9.38VHH16 pKa = 5.8GFRR19 pKa = 11.84KK20 pKa = 10.04RR21 pKa = 11.84MSTKK25 pKa = 9.46NGRR28 pKa = 11.84RR29 pKa = 11.84VLASRR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84KK37 pKa = 8.8GRR39 pKa = 11.84KK40 pKa = 8.76VLAAA44 pKa = 4.31

Molecular weight: 5.34 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4469

0

4469

1305096

20

2170

292.0

32.82

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.099 ± 0.038

0.752 ± 0.013

5.301 ± 0.033

7.434 ± 0.042

4.673 ± 0.03

6.553 ± 0.038

1.769 ± 0.016

7.764 ± 0.033

7.031 ± 0.042

9.925 ± 0.042

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.732 ± 0.02

4.616 ± 0.028

3.42 ± 0.021

4.082 ± 0.035

3.872 ± 0.026

6.032 ± 0.043

5.641 ± 0.027

6.769 ± 0.032

0.89 ± 0.013

3.646 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski