Tetranychus urticae (Two-spotted spider mite)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Protostomia; Ecdysozoa; Panarthropoda; Arthropoda; Chelicerata; Arachnida; Acari; Acariformes; Trombidiformes; Prostigmata; Eleutherengona; Raphignathae; Tetranychoidea; Tetranychidae; Tetranychus

Average proteome isoelectric point is 6.83

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 17526 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|T1K2X1|T1K2X1_TETUR VWFA domain-containing protein OS=Tetranychus urticae OX=32264 GN=107359743 PE=4 SV=1
MM1 pKa = 7.53WSSEE5 pKa = 4.08CGLTITKK12 pKa = 7.8EE13 pKa = 4.39TVDD16 pKa = 3.88GLSKK20 pKa = 10.84GSRR23 pKa = 11.84LLIDD27 pKa = 4.29LFDD30 pKa = 4.0YY31 pKa = 10.64VYY33 pKa = 11.06KK34 pKa = 10.96AIGCRR39 pKa = 11.84YY40 pKa = 9.61SPDD43 pKa = 3.42GGPEE47 pKa = 3.99FGVKK51 pKa = 9.91DD52 pKa = 3.92CSVLLVSLYY61 pKa = 10.53TGDD64 pKa = 5.34AIPDD68 pKa = 3.54AGADD72 pKa = 3.54LSIGVTVVVEE82 pKa = 4.07EE83 pKa = 4.7DD84 pKa = 3.75GEE86 pKa = 4.57GALLVDD92 pKa = 4.29VNVDD96 pKa = 3.02ADD98 pKa = 4.1GEE100 pKa = 4.52VALFGVTIQSTGILVYY116 pKa = 10.84

Molecular weight: 12.14 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|T1JQ83|T1JQ83_TETUR Uncharacterized protein OS=Tetranychus urticae OX=32264 PE=4 SV=1
MM1 pKa = 7.05MRR3 pKa = 11.84AKK5 pKa = 9.56TLRR8 pKa = 11.84SGRR11 pKa = 11.84QTISGHH17 pKa = 4.49VLLNRR22 pKa = 11.84RR23 pKa = 11.84KK24 pKa = 8.74QQAVFIGPRR33 pKa = 11.84RR34 pKa = 11.84SSVV37 pKa = 2.81

Molecular weight: 4.24 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

17526

0

17526

6781689

20

18253

387.0

43.68

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.247 ± 0.017

2.003 ± 0.015

5.568 ± 0.016

5.869 ± 0.026

4.564 ± 0.018

5.153 ± 0.022

2.398 ± 0.011

6.899 ± 0.018

6.64 ± 0.021

9.336 ± 0.027

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.118 ± 0.008

5.813 ± 0.023

4.766 ± 0.022

3.804 ± 0.017

4.628 ± 0.014

9.414 ± 0.039

5.661 ± 0.021

5.563 ± 0.013

1.077 ± 0.007

3.457 ± 0.014
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski