Geobacillus sp. CAMR12739
Average proteome isoelectric point is 6.82
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1702 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A063YJL0|A0A063YJL0_9BACI Uncharacterized protein OS=Geobacillus sp. CAMR12739 OX=1482738 GN=DI43_18650 PE=4 SV=1
MM1 pKa = 7.28 EE2 pKa = 5.7 HH3 pKa = 7.27 GDD5 pKa = 3.4 RR6 pKa = 11.84 HH7 pKa = 5.08 ITVVDD12 pKa = 3.13 EE13 pKa = 4.8 HH14 pKa = 8.52 GNEE17 pKa = 4.01 QLCEE21 pKa = 3.68 ILFTFEE27 pKa = 4.68 SDD29 pKa = 4.23 DD30 pKa = 3.72 FGKK33 pKa = 10.65 SYY35 pKa = 11.12 VFYY38 pKa = 10.84 YY39 pKa = 10.0 PVGVEE44 pKa = 4.67 AEE46 pKa = 4.32 DD47 pKa = 3.86 EE48 pKa = 4.17 NGEE51 pKa = 4.45 TEE53 pKa = 4.22 VHH55 pKa = 5.84 VSAFIPGDD63 pKa = 3.56 EE64 pKa = 4.22 NKK66 pKa = 10.39 EE67 pKa = 4.09 GEE69 pKa = 4.44 LLPIEE74 pKa = 4.66 TEE76 pKa = 4.37 EE77 pKa = 3.97 EE78 pKa = 3.3 WDD80 pKa = 3.66 MIEE83 pKa = 4.42 EE84 pKa = 4.17 VWNTFCAEE92 pKa = 3.99 QEE94 pKa = 4.29 EE95 pKa = 4.64 EE96 pKa = 4.38 DD97 pKa = 5.22 DD98 pKa = 4.01 EE99 pKa = 4.62
Molecular weight: 11.52 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.765
IPC2_protein 3.732
IPC_protein 3.668
Toseland 3.503
ProMoST 3.77
Dawson 3.617
Bjellqvist 3.821
Wikipedia 3.49
Rodwell 3.503
Grimsley 3.414
Solomon 3.605
Lehninger 3.554
Nozaki 3.745
DTASelect 3.846
Thurlkill 3.528
EMBOSS 3.516
Sillero 3.783
Patrickios 0.896
IPC_peptide 3.605
IPC2_peptide 3.757
IPC2.peptide.svr19 3.706
Protein with the highest isoelectric point:
>tr|A0A063YLP0|A0A063YLP0_9BACI Competence protein ComF OS=Geobacillus sp. CAMR12739 OX=1482738 GN=DI43_17410 PE=4 SV=1
MM1 pKa = 7.35 KK2 pKa = 9.4 RR3 pKa = 11.84 TYY5 pKa = 10.21 QPNRR9 pKa = 11.84 RR10 pKa = 11.84 KK11 pKa = 9.75 RR12 pKa = 11.84 SKK14 pKa = 9.65 VHH16 pKa = 5.81 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MSTRR25 pKa = 11.84 NGRR28 pKa = 11.84 KK29 pKa = 8.3 VLARR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 RR36 pKa = 11.84 KK37 pKa = 9.05 GRR39 pKa = 11.84 KK40 pKa = 8.7 VLSAA44 pKa = 4.05
Molecular weight: 5.35 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.464
IPC2_protein 11.184
IPC_protein 12.588
Toseland 12.749
ProMoST 13.247
Dawson 12.749
Bjellqvist 12.749
Wikipedia 13.217
Rodwell 12.427
Grimsley 12.793
Solomon 13.247
Lehninger 13.144
Nozaki 12.749
DTASelect 12.749
Thurlkill 12.749
EMBOSS 13.247
Sillero 12.749
Patrickios 12.149
IPC_peptide 13.247
IPC2_peptide 12.237
IPC2.peptide.svr19 9.093
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1702
0
1702
327887
32
815
192.6
21.6
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.182 ± 0.087
0.956 ± 0.026
4.841 ± 0.046
7.361 ± 0.076
4.182 ± 0.052
7.133 ± 0.063
2.358 ± 0.039
6.326 ± 0.055
5.55 ± 0.056
9.712 ± 0.074
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.799 ± 0.031
3.187 ± 0.044
4.232 ± 0.045
3.653 ± 0.047
6.383 ± 0.069
4.989 ± 0.06
5.035 ± 0.052
7.545 ± 0.064
1.265 ± 0.028
3.309 ± 0.044
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here