Blochmannia endosymbiont of Camponotus (Colobopsis) obliquus

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Enterobacteriaceae incertae sedis; ant, tsetse, mealybug, aphid, etc. endosymbionts; ant endosymbionts; Candidatus Blochmannia; unclassified Candidatus Blochmannia endosymbionts

Average proteome isoelectric point is 7.86

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 584 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0E3Y7R3|A0A0E3Y7R3_9ENTR Lipopolysaccharide export system permease protein LptF OS=Blochmannia endosymbiont of Camponotus (Colobopsis) obliquus OX=1505597 GN=lptF PE=3 SV=1
MM1 pKa = 7.66EE2 pKa = 5.63GLNIDD7 pKa = 4.7LLYY10 pKa = 10.53ISAAIMMGLAAIGAAIGIGILGGKK34 pKa = 9.02FLEE37 pKa = 5.19CAARR41 pKa = 11.84QPDD44 pKa = 4.86LIDD47 pKa = 3.73LLRR50 pKa = 11.84TQFFIVMGLVDD61 pKa = 6.96AIPMITVGLGLYY73 pKa = 10.79VMFAITT79 pKa = 4.2

Molecular weight: 8.33 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0E3Y748|A0A0E3Y748_9ENTR Carbamoyl-phosphate synthase small chain OS=Blochmannia endosymbiont of Camponotus (Colobopsis) obliquus OX=1505597 GN=carA PE=3 SV=1
MM1 pKa = 7.45KK2 pKa = 9.56RR3 pKa = 11.84TFQPSRR9 pKa = 11.84LKK11 pKa = 10.71RR12 pKa = 11.84NRR14 pKa = 11.84THH16 pKa = 7.05GFRR19 pKa = 11.84IRR21 pKa = 11.84MATKK25 pKa = 9.95NGRR28 pKa = 11.84QILLRR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.2GRR39 pKa = 11.84SRR41 pKa = 11.84ITVCCC46 pKa = 3.87

Molecular weight: 5.56 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

584

0

584

190803

38

1413

326.7

37.09

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.268 ± 0.089

1.632 ± 0.038

4.51 ± 0.064

4.648 ± 0.074

4.544 ± 0.072

6.019 ± 0.096

2.473 ± 0.045

10.888 ± 0.114

7.142 ± 0.099

10.428 ± 0.118

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.536 ± 0.04

6.521 ± 0.11

3.174 ± 0.041

3.764 ± 0.053

4.443 ± 0.083

6.403 ± 0.07

4.734 ± 0.068

6.069 ± 0.104

1.028 ± 0.031

3.777 ± 0.067
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski