Rose yellow vein virus
Average proteome isoelectric point is 7.02
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 8 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|I7CPB4|I7CPB4_9VIRU Uncharacterized protein OS=Rose yellow vein virus OX=1213588 PE=4 SV=1
MM1 pKa = 7.71 SIRR4 pKa = 11.84 RR5 pKa = 11.84 QEE7 pKa = 3.77 VTKK10 pKa = 10.58 AIQLATSNLTDD21 pKa = 3.2 KK22 pKa = 11.0 VLYY25 pKa = 10.12 LDD27 pKa = 4.06 QIGITEE33 pKa = 4.2 PSFNEE38 pKa = 3.69 QVNFLGNLSKK48 pKa = 9.94 TNQYY52 pKa = 10.0 VSGVILLKK60 pKa = 10.8 LEE62 pKa = 3.91 QLEE65 pKa = 4.22 EE66 pKa = 4.06 QIRR69 pKa = 11.84 AQTIDD74 pKa = 3.28 IQNLDD79 pKa = 3.52 KK80 pKa = 11.08 KK81 pKa = 10.69 LSKK84 pKa = 10.74 EE85 pKa = 3.9 KK86 pKa = 10.71 GIEE89 pKa = 4.14 SSDD92 pKa = 3.85 LDD94 pKa = 4.0 PLLEE98 pKa = 4.3 QIKK101 pKa = 9.74 EE102 pKa = 4.04 LNISKK107 pKa = 10.32 DD108 pKa = 3.59 VQQLSRR114 pKa = 11.84 DD115 pKa = 3.68 LEE117 pKa = 4.32 SLKK120 pKa = 11.04 GEE122 pKa = 4.32 TSIQPQFGWMTEE134 pKa = 3.86 GKK136 pKa = 10.39 ADD138 pKa = 3.55 LFSYY142 pKa = 10.47 RR143 pKa = 11.84 KK144 pKa = 9.58 VKK146 pKa = 10.62 PGDD149 pKa = 3.72 SSSLPLSLKK158 pKa = 10.7 DD159 pKa = 3.63 KK160 pKa = 10.73 EE161 pKa = 4.53 EE162 pKa = 4.59 SSSQQ166 pKa = 3.23
Molecular weight: 18.73 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.036
IPC2_protein 4.991
IPC_protein 4.863
Toseland 4.711
ProMoST 4.94
Dawson 4.787
Bjellqvist 4.94
Wikipedia 4.635
Rodwell 4.685
Grimsley 4.609
Solomon 4.787
Lehninger 4.736
Nozaki 4.889
DTASelect 5.029
Thurlkill 4.698
EMBOSS 4.66
Sillero 4.952
Patrickios 4.571
IPC_peptide 4.787
IPC2_peptide 4.952
IPC2.peptide.svr19 4.968
Protein with the highest isoelectric point:
>tr|I7E3F7|I7E3F7_9VIRU Uncharacterized protein OS=Rose yellow vein virus OX=1213588 PE=4 SV=1
MM1 pKa = 7.48 FANCLNFLRR10 pKa = 11.84 EE11 pKa = 4.05 IKK13 pKa = 10.21 QVLSLLLQLSKK24 pKa = 10.93 KK25 pKa = 10.34 ALFMSQLVFLTRR37 pKa = 11.84 LIVDD41 pKa = 5.29 LIYY44 pKa = 11.12 VSNNHH49 pKa = 6.17 FKK51 pKa = 10.75 PPLRR55 pKa = 11.84 ANPRR59 pKa = 11.84 VPTEE63 pKa = 3.12 IHH65 pKa = 5.53 QRR67 pKa = 11.84 NNSNEE72 pKa = 3.98 YY73 pKa = 9.5 PDD75 pKa = 5.75 SDD77 pKa = 4.3 IDD79 pKa = 3.3 WSTISLNN86 pKa = 3.56
Molecular weight: 10.04 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.398
IPC2_protein 8.17
IPC_protein 8.507
Toseland 8.873
ProMoST 8.624
Dawson 9.019
Bjellqvist 8.975
Wikipedia 9.092
Rodwell 9.092
Grimsley 8.712
Solomon 9.428
Lehninger 9.414
Nozaki 9.136
DTASelect 8.873
Thurlkill 8.96
EMBOSS 9.238
Sillero 9.18
Patrickios 5.041
IPC_peptide 9.414
IPC2_peptide 7.922
IPC2.peptide.svr19 7.665
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
8
0
8
2692
86
819
336.5
38.75
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
4.792 ± 0.372
1.077 ± 0.334
4.903 ± 0.363
8.247 ± 0.46
4.495 ± 0.248
3.752 ± 0.287
2.08 ± 0.43
7.764 ± 0.481
9.138 ± 0.73
9.77 ± 0.684
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.895 ± 0.236
5.684 ± 0.273
4.755 ± 0.5
5.312 ± 0.527
4.235 ± 0.206
8.544 ± 0.495
4.458 ± 0.33
4.643 ± 0.511
1.337 ± 0.233
3.12 ± 0.46
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here