Pantoea wallisii
Average proteome isoelectric point is 6.59
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3765 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1X1DC84|A0A1X1DC84_9GAMM AAA_13 domain-containing protein OS=Pantoea wallisii OX=1076551 GN=HA48_04385 PE=4 SV=1
MM1 pKa = 7.52 QYY3 pKa = 10.37 EE4 pKa = 4.04 DD5 pKa = 3.93 LYY7 pKa = 11.42 RR8 pKa = 11.84 RR9 pKa = 11.84 IAQIIFSCGPDD20 pKa = 3.64 GARR23 pKa = 11.84 MLIVQAEE30 pKa = 4.38 LFADD34 pKa = 3.82 EE35 pKa = 6.41 DD36 pKa = 3.81 GGQYY40 pKa = 10.05 QFDD43 pKa = 4.44 YY44 pKa = 10.43 IDD46 pKa = 3.95 EE47 pKa = 4.56 KK48 pKa = 11.67 GEE50 pKa = 4.2 PDD52 pKa = 3.51 WFEE55 pKa = 4.04 PDD57 pKa = 2.79 ARR59 pKa = 11.84 AIGDD63 pKa = 3.58 LTVALKK69 pKa = 10.74 AFQQYY74 pKa = 8.96 FVDD77 pKa = 4.18 NDD79 pKa = 3.54 MTQGQPVWTHH89 pKa = 4.77 CTITVDD95 pKa = 3.72 VEE97 pKa = 4.1 QEE99 pKa = 4.09 AISIDD104 pKa = 3.77 VQQ106 pKa = 3.42
Molecular weight: 12.15 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.736
IPC2_protein 3.783
IPC_protein 3.757
Toseland 3.541
ProMoST 3.935
Dawson 3.757
Bjellqvist 3.91
Wikipedia 3.706
Rodwell 3.592
Grimsley 3.452
Solomon 3.745
Lehninger 3.694
Nozaki 3.884
DTASelect 4.101
Thurlkill 3.605
EMBOSS 3.706
Sillero 3.884
Patrickios 1.875
IPC_peptide 3.732
IPC2_peptide 3.859
IPC2.peptide.svr19 3.81
Protein with the highest isoelectric point:
>tr|A0A1X1D4B3|A0A1X1D4B3_9GAMM Site-specific DNA-methyltransferase (adenine-specific) OS=Pantoea wallisii OX=1076551 GN=HA48_15585 PE=3 SV=1
MM1 pKa = 7.16 VARR4 pKa = 11.84 AGQPSGWPVSFVAGFSPPSGLPPKK28 pKa = 10.34 RR29 pKa = 11.84 EE30 pKa = 4.03 NFCGSSFQLTKK41 pKa = 10.58 EE42 pKa = 4.08 LFIMAVIFCTSRR54 pKa = 11.84 SEE56 pKa = 4.0 FTFIFAAIRR65 pKa = 11.84 RR66 pKa = 11.84 TDD68 pKa = 3.24 AAARR72 pKa = 11.84 PCMLRR77 pKa = 11.84 TVAGDD82 pKa = 3.47 EE83 pKa = 4.03 RR84 pKa = 11.84 SARR87 pKa = 11.84 ASLARR92 pKa = 11.84 DD93 pKa = 3.49 YY94 pKa = 11.33 VLSFAGRR101 pKa = 11.84 LPVKK105 pKa = 10.43 AVAAA109 pKa = 4.31
Molecular weight: 11.76 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.348
IPC2_protein 9.37
IPC_protein 10.101
Toseland 10.57
ProMoST 10.262
Dawson 10.657
Bjellqvist 10.394
Wikipedia 10.862
Rodwell 10.76
Grimsley 10.687
Solomon 10.804
Lehninger 10.774
Nozaki 10.613
DTASelect 10.365
Thurlkill 10.57
EMBOSS 10.979
Sillero 10.599
Patrickios 10.599
IPC_peptide 10.818
IPC2_peptide 9.794
IPC2.peptide.svr19 8.5
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3765
0
3765
1157890
29
2019
307.5
33.91
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
10.592 ± 0.05
0.981 ± 0.015
5.158 ± 0.033
5.347 ± 0.04
3.779 ± 0.028
7.452 ± 0.044
2.376 ± 0.02
5.497 ± 0.032
3.73 ± 0.036
11.212 ± 0.056
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.696 ± 0.019
3.485 ± 0.031
4.498 ± 0.029
4.985 ± 0.037
5.958 ± 0.034
5.896 ± 0.033
5.191 ± 0.024
6.998 ± 0.03
1.541 ± 0.019
2.63 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here