Gordonia phage CaptainKirk2

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Fairfaxidumvirus; unclassified Fairfaxidumvirus

Average proteome isoelectric point is 6.15

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 79 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1B3AZ02|A0A1B3AZ02_9CAUD Uncharacterized protein OS=Gordonia phage CaptainKirk2 OX=1887643 GN=56 PE=4 SV=1
MM1 pKa = 7.46ANDD4 pKa = 4.61TIFEE8 pKa = 4.28LPEE11 pKa = 4.65IPGVTFTASYY21 pKa = 10.84GSGGEE26 pKa = 4.12TGLPSNWIRR35 pKa = 11.84IVGTVEE41 pKa = 3.86NPWYY45 pKa = 10.31DD46 pKa = 3.26PTYY49 pKa = 11.17NYY51 pKa = 11.17GLDD54 pKa = 3.95PNGHH58 pKa = 5.7TEE60 pKa = 4.3VTDD63 pKa = 3.08PWKK66 pKa = 10.68RR67 pKa = 11.84HH68 pKa = 3.86TQFPEE73 pKa = 3.62VLGPMGFEE81 pKa = 4.55GPSIGLPVDD90 pKa = 3.94PPPPPVEE97 pKa = 4.39PEE99 pKa = 3.83PTPDD103 pKa = 3.7IIEE106 pKa = 4.12EE107 pKa = 4.27HH108 pKa = 6.37TDD110 pKa = 3.11GG111 pKa = 5.26

Molecular weight: 12.1 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1B3AZ19|A0A1B3AZ19_9CAUD RuvC-like resolvase OS=Gordonia phage CaptainKirk2 OX=1887643 GN=75 PE=4 SV=1
MM1 pKa = 7.09TSQVIEE7 pKa = 4.19DD8 pKa = 5.1WITHH12 pKa = 6.57LYY14 pKa = 10.98AEE16 pKa = 4.96GKK18 pKa = 9.64SNRR21 pKa = 11.84TVKK24 pKa = 10.42DD25 pKa = 3.07RR26 pKa = 11.84RR27 pKa = 11.84IVLRR31 pKa = 11.84RR32 pKa = 11.84FEE34 pKa = 4.58RR35 pKa = 11.84DD36 pKa = 2.39IGTPILAANTGQIAAWLGRR55 pKa = 11.84DD56 pKa = 3.57DD57 pKa = 4.33LASVTRR63 pKa = 11.84SVYY66 pKa = 10.59HH67 pKa = 6.65SILTAFYY74 pKa = 9.21RR75 pKa = 11.84WAIAVKK81 pKa = 10.41LRR83 pKa = 11.84DD84 pKa = 4.56DD85 pKa = 4.22NPVAPIKK92 pKa = 10.04AAKK95 pKa = 9.6RR96 pKa = 11.84PRR98 pKa = 11.84RR99 pKa = 11.84QPRR102 pKa = 11.84PVTPEE107 pKa = 3.31QYY109 pKa = 10.32RR110 pKa = 11.84RR111 pKa = 11.84LLEE114 pKa = 4.34GASDD118 pKa = 4.17DD119 pKa = 4.15PVTTAMLLLAGMQGLRR135 pKa = 11.84VHH137 pKa = 6.47EE138 pKa = 4.26VARR141 pKa = 11.84FHH143 pKa = 7.23SRR145 pKa = 11.84HH146 pKa = 6.22LDD148 pKa = 3.29VEE150 pKa = 4.06ARR152 pKa = 11.84TIEE155 pKa = 4.04VTGKK159 pKa = 10.41GGSSYY164 pKa = 11.01VLPAAQPVIDD174 pKa = 3.98HH175 pKa = 7.29AKK177 pKa = 10.27RR178 pKa = 11.84MPQAGFWFPSQRR190 pKa = 11.84AKK192 pKa = 10.86HH193 pKa = 5.51LGGRR197 pKa = 11.84TVSEE201 pKa = 4.67RR202 pKa = 11.84IHH204 pKa = 7.67LHH206 pKa = 5.49MIRR209 pKa = 11.84HH210 pKa = 5.78RR211 pKa = 11.84VPGTPHH217 pKa = 7.29CLRR220 pKa = 11.84HH221 pKa = 5.95HH222 pKa = 7.24FGTQLVSNGADD233 pKa = 2.95LRR235 pKa = 11.84VAQEE239 pKa = 4.07LLRR242 pKa = 11.84HH243 pKa = 5.97SSLQTTAIYY252 pKa = 10.64VATSDD257 pKa = 3.5DD258 pKa = 3.86RR259 pKa = 11.84KK260 pKa = 10.58RR261 pKa = 11.84EE262 pKa = 4.36AIDD265 pKa = 3.28RR266 pKa = 11.84LAAA269 pKa = 4.24

Molecular weight: 30.32 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

79

0

79

15513

47

1847

196.4

21.38

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.829 ± 0.463

0.812 ± 0.145

6.936 ± 0.27

5.563 ± 0.294

2.675 ± 0.16

8.432 ± 0.362

2.108 ± 0.191

4.551 ± 0.155

3.41 ± 0.235

7.735 ± 0.272

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.237 ± 0.178

2.869 ± 0.172

5.898 ± 0.246

3.313 ± 0.152

7.465 ± 0.418

5.325 ± 0.166

6.73 ± 0.224

7.864 ± 0.234

2.056 ± 0.118

2.192 ± 0.177
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski