Amino acid dipepetide frequency for Gordonia phage CaptainKirk2

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
17.986AlaAla: 17.986 ± 1.456
0.645AlaCys: 0.645 ± 0.249
7.8AlaAsp: 7.8 ± 0.821
6.64AlaGlu: 6.64 ± 0.662
3.804AlaPhe: 3.804 ± 0.57
9.412AlaGly: 9.412 ± 1.015
1.998AlaHis: 1.998 ± 0.385
5.609AlaIle: 5.609 ± 0.495
3.739AlaLys: 3.739 ± 0.673
10.959AlaLeu: 10.959 ± 1.161
2.965AlaMet: 2.965 ± 0.582
3.546AlaAsn: 3.546 ± 0.662
4.835AlaPro: 4.835 ± 0.502
4.319AlaGln: 4.319 ± 0.661
8.832AlaArg: 8.832 ± 0.852
5.802AlaSer: 5.802 ± 0.761
7.478AlaThr: 7.478 ± 0.787
7.8AlaVal: 7.8 ± 1.107
2.127AlaTrp: 2.127 ± 0.322
1.998AlaTyr: 1.998 ± 0.327
0.0AlaXaa: 0.0 ± 0.0
Cys
0.451CysAla: 0.451 ± 0.176
0.129CysCys: 0.129 ± 0.121
1.096CysAsp: 1.096 ± 0.342
0.258CysGlu: 0.258 ± 0.143
0.0CysPhe: 0.0 ± 0.0
0.645CysGly: 0.645 ± 0.219
0.516CysHis: 0.516 ± 0.193
0.064CysIle: 0.064 ± 0.055
0.129CysLys: 0.129 ± 0.101
0.774CysLeu: 0.774 ± 0.254
0.258CysMet: 0.258 ± 0.153
0.258CysAsn: 0.258 ± 0.143
0.838CysPro: 0.838 ± 0.251
0.387CysGln: 0.387 ± 0.166
0.58CysArg: 0.58 ± 0.23
0.451CysSer: 0.451 ± 0.189
0.709CysThr: 0.709 ± 0.258
0.322CysVal: 0.322 ± 0.152
0.258CysTrp: 0.258 ± 0.148
0.0CysTyr: 0.0 ± 0.0
0.0CysXaa: 0.0 ± 0.0
Asp
7.22AspAla: 7.22 ± 0.764
0.322AspCys: 0.322 ± 0.154
5.222AspAsp: 5.222 ± 0.639
4.771AspGlu: 4.771 ± 0.67
1.741AspPhe: 1.741 ± 0.345
7.027AspGly: 7.027 ± 0.869
1.934AspHis: 1.934 ± 0.36
2.965AspIle: 2.965 ± 0.469
1.676AspLys: 1.676 ± 0.355
5.995AspLeu: 5.995 ± 0.803
1.289AspMet: 1.289 ± 0.284
1.998AspAsn: 1.998 ± 0.447
4.319AspPro: 4.319 ± 0.601
2.643AspGln: 2.643 ± 0.335
4.706AspArg: 4.706 ± 0.65
3.481AspSer: 3.481 ± 0.377
3.997AspThr: 3.997 ± 0.508
5.351AspVal: 5.351 ± 0.7
0.967AspTrp: 0.967 ± 0.243
1.741AspTyr: 1.741 ± 0.377
0.0AspXaa: 0.0 ± 0.0
Glu
5.351GluAla: 5.351 ± 0.613
0.451GluCys: 0.451 ± 0.184
3.03GluAsp: 3.03 ± 0.393
2.192GluGlu: 2.192 ± 0.469
1.934GluPhe: 1.934 ± 0.372
4.061GluGly: 4.061 ± 0.497
1.289GluHis: 1.289 ± 0.252
2.514GluIle: 2.514 ± 0.359
1.998GluLys: 1.998 ± 0.311
5.028GluLeu: 5.028 ± 0.749
1.676GluMet: 1.676 ± 0.33
1.418GluAsn: 1.418 ± 0.293
3.997GluPro: 3.997 ± 0.664
3.288GluGln: 3.288 ± 0.612
3.932GluArg: 3.932 ± 0.529
2.643GluSer: 2.643 ± 0.336
2.45GluThr: 2.45 ± 0.336
5.673GluVal: 5.673 ± 0.728
1.16GluTrp: 1.16 ± 0.368
1.547GluTyr: 1.547 ± 0.411
0.0GluXaa: 0.0 ± 0.0
Phe
2.772PheAla: 2.772 ± 0.383
0.258PheCys: 0.258 ± 0.146
2.321PheAsp: 2.321 ± 0.286
1.741PheGlu: 1.741 ± 0.306
0.774PhePhe: 0.774 ± 0.218
2.321PheGly: 2.321 ± 0.452
0.387PheHis: 0.387 ± 0.204
0.774PheIle: 0.774 ± 0.265
1.031PheLys: 1.031 ± 0.307
1.483PheLeu: 1.483 ± 0.405
0.451PheMet: 0.451 ± 0.168
0.58PheAsn: 0.58 ± 0.213
1.612PhePro: 1.612 ± 0.26
0.709PheGln: 0.709 ± 0.164
1.805PheArg: 1.805 ± 0.311
1.676PheSer: 1.676 ± 0.398
2.256PheThr: 2.256 ± 0.372
2.965PheVal: 2.965 ± 0.388
0.193PheTrp: 0.193 ± 0.108
0.645PheTyr: 0.645 ± 0.186
0.0PheXaa: 0.0 ± 0.0
Gly
8.896GlyAla: 8.896 ± 1.187
0.451GlyCys: 0.451 ± 0.188
5.609GlyAsp: 5.609 ± 0.55
5.028GlyGlu: 5.028 ± 0.715
2.708GlyPhe: 2.708 ± 0.504
6.962GlyGly: 6.962 ± 0.921
1.676GlyHis: 1.676 ± 0.305
3.997GlyIle: 3.997 ± 0.715
3.417GlyLys: 3.417 ± 0.472
8.058GlyLeu: 8.058 ± 1.292
1.418GlyMet: 1.418 ± 0.259
2.256GlyAsn: 2.256 ± 0.331
3.932GlyPro: 3.932 ± 0.539
2.965GlyGln: 2.965 ± 0.371
6.576GlyArg: 6.576 ± 0.561
5.028GlySer: 5.028 ± 0.72
4.706GlyThr: 4.706 ± 0.551
5.931GlyVal: 5.931 ± 0.695
2.45GlyTrp: 2.45 ± 0.412
2.256GlyTyr: 2.256 ± 0.385
0.0GlyXaa: 0.0 ± 0.0
His
2.256HisAla: 2.256 ± 0.355
0.322HisCys: 0.322 ± 0.144
1.483HisAsp: 1.483 ± 0.323
0.903HisGlu: 0.903 ± 0.21
0.387HisPhe: 0.387 ± 0.187
1.612HisGly: 1.612 ± 0.35
0.645HisHis: 0.645 ± 0.197
0.903HisIle: 0.903 ± 0.265
0.58HisLys: 0.58 ± 0.225
1.998HisLeu: 1.998 ± 0.391
0.322HisMet: 0.322 ± 0.127
0.387HisAsn: 0.387 ± 0.179
1.87HisPro: 1.87 ± 0.335
0.709HisGln: 0.709 ± 0.195
1.547HisArg: 1.547 ± 0.304
1.16HisSer: 1.16 ± 0.289
1.16HisThr: 1.16 ± 0.301
1.805HisVal: 1.805 ± 0.4
0.516HisTrp: 0.516 ± 0.153
0.516HisTyr: 0.516 ± 0.18
0.0HisXaa: 0.0 ± 0.0
Ile
6.382IleAla: 6.382 ± 0.499
0.193IleCys: 0.193 ± 0.094
4.19IleAsp: 4.19 ± 0.641
2.256IleGlu: 2.256 ± 0.294
0.838IlePhe: 0.838 ± 0.234
4.577IleGly: 4.577 ± 0.753
0.903IleHis: 0.903 ± 0.222
1.483IleIle: 1.483 ± 0.386
1.676IleLys: 1.676 ± 0.434
2.514IleLeu: 2.514 ± 0.383
0.129IleMet: 0.129 ± 0.083
1.096IleAsn: 1.096 ± 0.221
3.094IlePro: 3.094 ± 0.389
0.774IleGln: 0.774 ± 0.236
4.061IleArg: 4.061 ± 0.492
2.063IleSer: 2.063 ± 0.379
3.739IleThr: 3.739 ± 0.438
4.448IleVal: 4.448 ± 0.53
0.258IleTrp: 0.258 ± 0.132
0.838IleTyr: 0.838 ± 0.196
0.0IleXaa: 0.0 ± 0.0
Lys
3.546LysAla: 3.546 ± 0.515
0.129LysCys: 0.129 ± 0.094
1.87LysAsp: 1.87 ± 0.57
1.289LysGlu: 1.289 ± 0.262
1.16LysPhe: 1.16 ± 0.289
2.385LysGly: 2.385 ± 0.432
0.451LysHis: 0.451 ± 0.201
1.612LysIle: 1.612 ± 0.395
2.063LysLys: 2.063 ± 0.313
3.223LysLeu: 3.223 ± 0.491
0.774LysMet: 0.774 ± 0.356
0.967LysAsn: 0.967 ± 0.321
2.837LysPro: 2.837 ± 0.335
1.225LysGln: 1.225 ± 0.338
2.063LysArg: 2.063 ± 0.393
1.934LysSer: 1.934 ± 0.342
2.514LysThr: 2.514 ± 0.473
2.579LysVal: 2.579 ± 0.359
0.709LysTrp: 0.709 ± 0.191
0.774LysTyr: 0.774 ± 0.215
0.0LysXaa: 0.0 ± 0.0
Leu
11.346LeuAla: 11.346 ± 0.931
0.838LeuCys: 0.838 ± 0.288
6.189LeuAsp: 6.189 ± 0.746
3.481LeuGlu: 3.481 ± 0.566
1.612LeuPhe: 1.612 ± 0.258
7.349LeuGly: 7.349 ± 0.76
1.031LeuHis: 1.031 ± 0.249
3.352LeuIle: 3.352 ± 0.531
1.805LeuLys: 1.805 ± 0.381
4.126LeuLeu: 4.126 ± 0.534
1.87LeuMet: 1.87 ± 0.419
2.192LeuAsn: 2.192 ± 0.385
4.319LeuPro: 4.319 ± 0.479
1.676LeuGln: 1.676 ± 0.31
5.415LeuArg: 5.415 ± 0.553
5.157LeuSer: 5.157 ± 0.695
5.544LeuThr: 5.544 ± 0.711
6.447LeuVal: 6.447 ± 0.712
2.063LeuTrp: 2.063 ± 0.372
1.547LeuTyr: 1.547 ± 0.263
0.0LeuXaa: 0.0 ± 0.0
Met
3.804MetAla: 3.804 ± 0.622
0.193MetCys: 0.193 ± 0.118
0.709MetAsp: 0.709 ± 0.226
0.645MetGlu: 0.645 ± 0.19
0.58MetPhe: 0.58 ± 0.178
1.676MetGly: 1.676 ± 0.35
0.451MetHis: 0.451 ± 0.187
1.096MetIle: 1.096 ± 0.27
0.58MetLys: 0.58 ± 0.22
1.612MetLeu: 1.612 ± 0.32
0.129MetMet: 0.129 ± 0.114
0.322MetAsn: 0.322 ± 0.12
1.934MetPro: 1.934 ± 0.343
0.709MetGln: 0.709 ± 0.271
1.934MetArg: 1.934 ± 0.581
1.934MetSer: 1.934 ± 0.411
2.579MetThr: 2.579 ± 0.387
0.516MetVal: 0.516 ± 0.237
0.645MetTrp: 0.645 ± 0.218
0.322MetTyr: 0.322 ± 0.138
0.0MetXaa: 0.0 ± 0.0
Asn
2.45AsnAla: 2.45 ± 0.492
0.258AsnCys: 0.258 ± 0.117
1.805AsnAsp: 1.805 ± 0.311
1.354AsnGlu: 1.354 ± 0.256
0.451AsnPhe: 0.451 ± 0.16
3.352AsnGly: 3.352 ± 0.475
0.645AsnHis: 0.645 ± 0.184
0.967AsnIle: 0.967 ± 0.263
0.838AsnLys: 0.838 ± 0.275
2.127AsnLeu: 2.127 ± 0.358
0.58AsnMet: 0.58 ± 0.174
0.516AsnAsn: 0.516 ± 0.191
3.094AsnPro: 3.094 ± 0.406
0.967AsnGln: 0.967 ± 0.212
2.063AsnArg: 2.063 ± 0.457
1.676AsnSer: 1.676 ± 0.333
2.385AsnThr: 2.385 ± 0.453
1.805AsnVal: 1.805 ± 0.375
0.451AsnTrp: 0.451 ± 0.139
0.903AsnTyr: 0.903 ± 0.239
0.0AsnXaa: 0.0 ± 0.0
Pro
6.833ProAla: 6.833 ± 0.9
0.838ProCys: 0.838 ± 0.298
4.126ProAsp: 4.126 ± 0.507
4.319ProGlu: 4.319 ± 0.77
1.612ProPhe: 1.612 ± 0.329
4.964ProGly: 4.964 ± 0.605
1.16ProHis: 1.16 ± 0.283
3.288ProIle: 3.288 ± 0.522
2.772ProLys: 2.772 ± 0.332
2.965ProLeu: 2.965 ± 0.482
1.612ProMet: 1.612 ± 0.476
2.256ProAsn: 2.256 ± 0.377
3.223ProPro: 3.223 ± 0.613
1.805ProGln: 1.805 ± 0.296
3.481ProArg: 3.481 ± 0.557
3.352ProSer: 3.352 ± 0.366
3.739ProThr: 3.739 ± 0.619
4.319ProVal: 4.319 ± 0.518
1.16ProTrp: 1.16 ± 0.236
1.16ProTyr: 1.16 ± 0.344
0.0ProXaa: 0.0 ± 0.0
Gln
3.546GlnAla: 3.546 ± 0.533
0.193GlnCys: 0.193 ± 0.117
1.096GlnAsp: 1.096 ± 0.248
1.418GlnGlu: 1.418 ± 0.277
0.903GlnPhe: 0.903 ± 0.251
2.256GlnGly: 2.256 ± 0.416
1.031GlnHis: 1.031 ± 0.27
1.483GlnIle: 1.483 ± 0.303
0.838GlnLys: 0.838 ± 0.197
3.352GlnLeu: 3.352 ± 0.409
0.774GlnMet: 0.774 ± 0.238
1.096GlnAsn: 1.096 ± 0.27
2.127GlnPro: 2.127 ± 0.533
1.547GlnGln: 1.547 ± 0.448
2.965GlnArg: 2.965 ± 0.464
1.741GlnSer: 1.741 ± 0.392
2.192GlnThr: 2.192 ± 0.406
2.708GlnVal: 2.708 ± 0.415
0.838GlnTrp: 0.838 ± 0.228
1.031GlnTyr: 1.031 ± 0.211
0.0GlnXaa: 0.0 ± 0.0
Arg
8.187ArgAla: 8.187 ± 0.863
0.903ArgCys: 0.903 ± 0.281
5.995ArgAsp: 5.995 ± 0.605
4.513ArgGlu: 4.513 ± 0.614
1.934ArgPhe: 1.934 ± 0.345
5.995ArgGly: 5.995 ± 0.619
1.998ArgHis: 1.998 ± 0.387
3.868ArgIle: 3.868 ± 0.603
2.256ArgLys: 2.256 ± 0.299
5.415ArgLeu: 5.415 ± 0.571
2.385ArgMet: 2.385 ± 0.4
2.321ArgAsn: 2.321 ± 0.408
3.546ArgPro: 3.546 ± 0.552
2.579ArgGln: 2.579 ± 0.587
6.833ArgArg: 6.833 ± 1.02
3.61ArgSer: 3.61 ± 0.412
4.19ArgThr: 4.19 ± 0.49
5.351ArgVal: 5.351 ± 0.701
1.547ArgTrp: 1.547 ± 0.327
1.225ArgTyr: 1.225 ± 0.303
0.0ArgXaa: 0.0 ± 0.0
Ser
6.511SerAla: 6.511 ± 0.785
0.193SerCys: 0.193 ± 0.133
3.352SerAsp: 3.352 ± 0.414
3.546SerGlu: 3.546 ± 0.679
1.354SerPhe: 1.354 ± 0.298
5.222SerGly: 5.222 ± 0.651
0.645SerHis: 0.645 ± 0.182
2.965SerIle: 2.965 ± 0.524
1.676SerLys: 1.676 ± 0.288
3.481SerLeu: 3.481 ± 0.464
1.354SerMet: 1.354 ± 0.331
2.192SerAsn: 2.192 ± 0.514
2.965SerPro: 2.965 ± 0.456
1.354SerGln: 1.354 ± 0.343
3.61SerArg: 3.61 ± 0.559
1.87SerSer: 1.87 ± 0.366
4.255SerThr: 4.255 ± 0.586
4.319SerVal: 4.319 ± 0.59
1.483SerTrp: 1.483 ± 0.284
0.838SerTyr: 0.838 ± 0.236
0.0SerXaa: 0.0 ± 0.0
Thr
7.607ThrAla: 7.607 ± 0.717
0.387ThrCys: 0.387 ± 0.146
5.028ThrAsp: 5.028 ± 0.604
4.061ThrGlu: 4.061 ± 0.54
2.321ThrPhe: 2.321 ± 0.538
5.802ThrGly: 5.802 ± 0.871
1.418ThrHis: 1.418 ± 0.278
3.03ThrIle: 3.03 ± 0.517
2.643ThrLys: 2.643 ± 0.369
5.609ThrLeu: 5.609 ± 0.665
1.418ThrMet: 1.418 ± 0.241
1.934ThrAsn: 1.934 ± 0.455
3.932ThrPro: 3.932 ± 0.548
1.87ThrGln: 1.87 ± 0.349
4.19ThrArg: 4.19 ± 0.497
3.481ThrSer: 3.481 ± 0.509
4.255ThrThr: 4.255 ± 0.559
5.866ThrVal: 5.866 ± 0.646
1.354ThrTrp: 1.354 ± 0.303
1.096ThrTyr: 1.096 ± 0.289
0.0ThrXaa: 0.0 ± 0.0
Val
9.025ValAla: 9.025 ± 0.862
1.096ValCys: 1.096 ± 0.351
6.06ValAsp: 6.06 ± 0.745
4.964ValGlu: 4.964 ± 0.502
1.805ValPhe: 1.805 ± 0.381
5.737ValGly: 5.737 ± 0.709
1.547ValHis: 1.547 ± 0.309
4.061ValIle: 4.061 ± 0.509
2.708ValLys: 2.708 ± 0.439
5.222ValLeu: 5.222 ± 0.54
1.805ValMet: 1.805 ± 0.467
1.741ValAsn: 1.741 ± 0.319
3.997ValPro: 3.997 ± 0.51
2.45ValGln: 2.45 ± 0.38
5.931ValArg: 5.931 ± 0.745
3.546ValSer: 3.546 ± 0.529
5.995ValThr: 5.995 ± 0.69
7.091ValVal: 7.091 ± 0.682
1.612ValTrp: 1.612 ± 0.413
2.256ValTyr: 2.256 ± 0.319
0.0ValXaa: 0.0 ± 0.0
Trp
1.87TrpAla: 1.87 ± 0.357
0.258TrpCys: 0.258 ± 0.121
1.289TrpAsp: 1.289 ± 0.43
0.774TrpGlu: 0.774 ± 0.193
0.516TrpPhe: 0.516 ± 0.193
0.838TrpGly: 0.838 ± 0.249
0.58TrpHis: 0.58 ± 0.186
0.774TrpIle: 0.774 ± 0.218
0.774TrpLys: 0.774 ± 0.196
1.998TrpLeu: 1.998 ± 0.406
0.516TrpMet: 0.516 ± 0.19
0.903TrpAsn: 0.903 ± 0.388
1.354TrpPro: 1.354 ± 0.278
0.516TrpGln: 0.516 ± 0.151
1.741TrpArg: 1.741 ± 0.351
1.354TrpSer: 1.354 ± 0.268
1.483TrpThr: 1.483 ± 0.29
1.934TrpVal: 1.934 ± 0.334
0.451TrpTrp: 0.451 ± 0.137
0.645TrpTyr: 0.645 ± 0.215
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.256TyrAla: 2.256 ± 0.329
0.064TyrCys: 0.064 ± 0.075
1.096TyrAsp: 1.096 ± 0.244
1.483TyrGlu: 1.483 ± 0.351
0.322TyrPhe: 0.322 ± 0.177
2.127TyrGly: 2.127 ± 0.372
0.774TyrHis: 0.774 ± 0.215
0.709TyrIle: 0.709 ± 0.262
0.903TyrLys: 0.903 ± 0.237
1.418TyrLeu: 1.418 ± 0.307
0.58TyrMet: 0.58 ± 0.171
0.709TyrAsn: 0.709 ± 0.198
1.16TyrPro: 1.16 ± 0.263
0.645TyrGln: 0.645 ± 0.25
2.385TyrArg: 2.385 ± 0.509
1.289TyrSer: 1.289 ± 0.376
1.676TyrThr: 1.676 ± 0.286
1.418TyrVal: 1.418 ± 0.348
0.322TyrTrp: 0.322 ± 0.146
0.58TyrTyr: 0.58 ± 0.223
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 79 proteins (15513 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski