Neisseria bacilliformis ATCC BAA-1200
Average proteome isoelectric point is 7.39
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2966 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|F2BDH6|F2BDH6_9NEIS Uncharacterized protein OS=Neisseria bacilliformis ATCC BAA-1200 OX=888742 GN=HMPREF9123_1782 PE=4 SV=1
MM1 pKa = 6.99 NTLPYY6 pKa = 10.02 QPEE9 pKa = 3.54 IAARR13 pKa = 11.84 MLAGGEE19 pKa = 4.09 ANDD22 pKa = 4.76 TIRR25 pKa = 11.84 PDD27 pKa = 3.03 ARR29 pKa = 11.84 FIQTANGHH37 pKa = 6.1 WIAWHH42 pKa = 7.33 DD43 pKa = 4.16 PDD45 pKa = 4.54 TPAAVLPPDD54 pKa = 3.94 TPAGEE59 pKa = 3.9 PCFWVEE65 pKa = 3.93 GAEE68 pKa = 4.14 DD69 pKa = 3.7 LAEE72 pKa = 4.08 LVHH75 pKa = 5.91 MAEE78 pKa = 4.94 NGDD81 pKa = 3.93 FDD83 pKa = 5.36 HH84 pKa = 7.55 LDD86 pKa = 3.87 DD87 pKa = 6.04 ADD89 pKa = 5.55 DD90 pKa = 4.09 GTEE93 pKa = 4.37 PPPHH97 pKa = 6.68 TCTCGCAHH105 pKa = 7.53 DD106 pKa = 4.02
Molecular weight: 11.45 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.764
IPC2_protein 3.973
IPC_protein 3.935
Toseland 3.732
ProMoST 4.037
Dawson 3.935
Bjellqvist 4.126
Wikipedia 3.872
Rodwell 3.77
Grimsley 3.643
Solomon 3.923
Lehninger 3.872
Nozaki 4.05
DTASelect 4.279
Thurlkill 3.783
EMBOSS 3.872
Sillero 4.062
Patrickios 0.998
IPC_peptide 3.91
IPC2_peptide 4.037
IPC2.peptide.svr19 3.974
Protein with the highest isoelectric point:
>tr|F2BGB8|F2BGB8_9NEIS Uncharacterized protein OS=Neisseria bacilliformis ATCC BAA-1200 OX=888742 GN=HMPREF9123_2775 PE=4 SV=1
MM1 pKa = 7.19 FPAARR6 pKa = 11.84 QIRR9 pKa = 11.84 AFSQNPNRR17 pKa = 11.84 VRR19 pKa = 11.84 GRR21 pKa = 11.84 ATHH24 pKa = 6.2 SAQTTRR30 pKa = 11.84 GRR32 pKa = 11.84 LKK34 pKa = 9.43 TRR36 pKa = 11.84 KK37 pKa = 7.94 TGFQTASS44 pKa = 3.01
Molecular weight: 4.96 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.491
IPC2_protein 11.082
IPC_protein 12.691
Toseland 12.852
ProMoST 13.349
Dawson 12.852
Bjellqvist 12.852
Wikipedia 13.32
Rodwell 12.457
Grimsley 12.896
Solomon 13.349
Lehninger 13.247
Nozaki 12.852
DTASelect 12.852
Thurlkill 12.852
EMBOSS 13.349
Sillero 12.852
Patrickios 12.193
IPC_peptide 13.349
IPC2_peptide 12.34
IPC2.peptide.svr19 9.136
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2966
0
2966
711328
39
2626
239.8
26.35
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
12.226 ± 0.083
1.204 ± 0.02
5.046 ± 0.037
5.61 ± 0.054
4.066 ± 0.035
7.713 ± 0.055
2.211 ± 0.026
4.865 ± 0.039
4.764 ± 0.042
10.034 ± 0.068
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.354 ± 0.026
3.61 ± 0.038
4.905 ± 0.051
4.182 ± 0.037
6.525 ± 0.059
5.146 ± 0.044
5.168 ± 0.039
6.273 ± 0.048
1.282 ± 0.022
2.818 ± 0.028
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here