Aegilops tauschii subsp. strangulata (Goatgrass)

Taxonomy: cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliopsida; Mesangiospermae; Liliopsida; Petrosaviidae; commelinids; Poales; Poaceae; BOP clade; Pooideae; Triticodae; Triticeae; Triticinae; Aegilops; Aegilops tauschii

Average proteome isoelectric point is 6.86

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 214162 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A453A9N1|A0A453A9N1_AEGTS Protein kinase domain-containing protein OS=Aegilops tauschii subsp. strangulata OX=200361 PE=4 SV=1
MM1 pKa = 7.17GKK3 pKa = 10.26AYY5 pKa = 10.28RR6 pKa = 11.84KK7 pKa = 9.97KK8 pKa = 10.87LGVNKK13 pKa = 10.13AATPAGGKK21 pKa = 10.19GKK23 pKa = 10.49GAGGGNAKK31 pKa = 10.23RR32 pKa = 11.84SGGAAGAGGAGPGGGAKK49 pKa = 10.22GAGGAGWGGASTFSATYY66 pKa = 10.04HH67 pKa = 5.68STFQTAVNVTNEE79 pKa = 4.49YY80 pKa = 9.74YY81 pKa = 10.53NQWGNDD87 pKa = 3.44CSSDD91 pKa = 4.14DD92 pKa = 4.92DD93 pKa = 4.9GPPSAPADD101 pKa = 3.64DD102 pKa = 4.42STFEE106 pKa = 4.09TAVNDD111 pKa = 3.7TNEE114 pKa = 4.12YY115 pKa = 8.71YY116 pKa = 10.09QEE118 pKa = 4.49EE119 pKa = 4.32EE120 pKa = 4.83DD121 pKa = 4.48NNCSSPFAPADD132 pKa = 3.21HH133 pKa = 6.51STFEE137 pKa = 4.2TAVKK141 pKa = 10.2IMNEE145 pKa = 4.09HH146 pKa = 6.47YY147 pKa = 10.99DD148 pKa = 3.57DD149 pKa = 5.79DD150 pKa = 4.87EE151 pKa = 5.0NLGLSMDD158 pKa = 3.95SEE160 pKa = 4.72EE161 pKa = 5.36EE162 pKa = 4.38GVQDD166 pKa = 4.93AEE168 pKa = 4.44PVSDD172 pKa = 5.09ADD174 pKa = 5.29GEE176 pKa = 4.4GDD178 pKa = 4.18DD179 pKa = 5.42DD180 pKa = 5.36AGSGDD185 pKa = 4.1DD186 pKa = 5.13HH187 pKa = 8.31FGDD190 pKa = 4.96DD191 pKa = 5.5DD192 pKa = 5.48YY193 pKa = 11.97YY194 pKa = 11.61DD195 pKa = 5.53DD196 pKa = 6.76DD197 pKa = 4.42IAGCFDD203 pKa = 3.62AFYY206 pKa = 10.92DD207 pKa = 4.59PGDD210 pKa = 4.28AGDD213 pKa = 5.56GADD216 pKa = 4.3DD217 pKa = 3.21WWW219 pKa = 5.75

Molecular weight: 22.5 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A453SWJ1|A0A453SWJ1_AEGTS Isoform of A0A453SWM0 Uncharacterized protein OS=Aegilops tauschii subsp. strangulata OX=200361 PE=4 SV=1
LL1 pKa = 7.33PGIPAQVPRR10 pKa = 11.84LPPGLRR16 pKa = 11.84RR17 pKa = 11.84RR18 pKa = 11.84LRR20 pKa = 11.84RR21 pKa = 11.84ALRR24 pKa = 11.84RR25 pKa = 11.84LHH27 pKa = 6.38PLPLGAAPRR36 pKa = 11.84AALRR40 pKa = 11.84PPPPRR45 pKa = 11.84PLL47 pKa = 4.47

Molecular weight: 5.21 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

38981

175181

214162

72665765

21

5005

339.3

37.58

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.232 ± 0.007

1.959 ± 0.003

5.404 ± 0.004

6.066 ± 0.006

3.8 ± 0.004

6.904 ± 0.006

2.557 ± 0.002

4.742 ± 0.004

5.25 ± 0.005

9.774 ± 0.007

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.44 ± 0.003

3.698 ± 0.003

5.391 ± 0.006

3.685 ± 0.005

6.046 ± 0.005

8.437 ± 0.007

4.908 ± 0.003

6.703 ± 0.004

1.292 ± 0.002

2.705 ± 0.003
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski