Melipona quadrifasciata

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Protostomia; Ecdysozoa; Panarthropoda; Arthropoda; Mandibulata; Pancrustacea; Hexapoda; Insecta; Dicondylia; Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita; Aculeata; Apoidea; Apidae; Apinae;

Average proteome isoelectric point is 7.26

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 14253 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0N0BJL0|A0A0N0BJL0_9HYME Uncharacterized protein OS=Melipona quadrifasciata OX=166423 GN=WN51_07174 PE=4 SV=1
MM1 pKa = 7.58LCNSEE6 pKa = 4.1TDD8 pKa = 3.43RR9 pKa = 11.84DD10 pKa = 3.96SKK12 pKa = 10.06TFQILYY18 pKa = 8.38LTLFLFCTLIHH29 pKa = 6.53LSIASFVSDD38 pKa = 3.25TNYY41 pKa = 8.75NTTLTNDD48 pKa = 3.25TSNNSTGDD56 pKa = 3.34DD57 pKa = 3.88LYY59 pKa = 10.63IIKK62 pKa = 10.8AMVYY66 pKa = 9.89EE67 pKa = 4.31IGILTDD73 pKa = 4.2ADD75 pKa = 3.61NATNDD80 pKa = 3.21TTEE83 pKa = 3.95RR84 pKa = 11.84QEE86 pKa = 4.25VKK88 pKa = 10.57LSFYY92 pKa = 11.06NPPTEE97 pKa = 5.22DD98 pKa = 3.14NGSS101 pKa = 3.27

Molecular weight: 11.35 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0N0BJD6|A0A0N0BJD6_9HYME Solute carrier family 35 member F5 OS=Melipona quadrifasciata OX=166423 GN=WN51_08607 PE=4 SV=1
QQ1 pKa = 7.1SAHH4 pKa = 5.21KK5 pKa = 9.46TFIIKK10 pKa = 10.29RR11 pKa = 11.84KK12 pKa = 8.65LAKK15 pKa = 9.89KK16 pKa = 10.19LKK18 pKa = 8.64QNRR21 pKa = 11.84PIPQWVRR28 pKa = 11.84MRR30 pKa = 11.84TGNTIRR36 pKa = 11.84YY37 pKa = 5.79NAKK40 pKa = 8.47RR41 pKa = 11.84RR42 pKa = 11.84HH43 pKa = 4.1WRR45 pKa = 11.84RR46 pKa = 11.84TKK48 pKa = 10.89LKK50 pKa = 10.55LL51 pKa = 3.39

Molecular weight: 6.35 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

14253

0

14253

6552381

26

21286

459.7

51.91

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.846 ± 0.02

2.056 ± 0.039

5.155 ± 0.016

6.892 ± 0.039

3.673 ± 0.015

5.336 ± 0.025

2.444 ± 0.011

5.934 ± 0.019

6.643 ± 0.03

8.954 ± 0.028

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.177 ± 0.01

5.258 ± 0.023

4.9 ± 0.027

4.209 ± 0.021

5.926 ± 0.021

8.252 ± 0.033

6.042 ± 0.019

6.008 ± 0.018

1.081 ± 0.007

3.116 ± 0.014
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski