Amino acid dipepetide frequency for Melipona quadrifasciata

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	4.826AlaAla: 4.826 ± 0.056	1.164AlaCys: 1.164 ± 0.028	2.7AlaAsp: 2.7 ± 0.022	3.72AlaGlu: 3.72 ± 0.037	2.045AlaPhe: 2.045 ± 0.023	3.334AlaGly: 3.334 ± 0.039	1.254AlaHis: 1.254 ± 0.016	3.449AlaIle: 3.449 ± 0.025	3.594AlaLys: 3.594 ± 0.043	5.344AlaLeu: 5.344 ± 0.041	1.368AlaMet: 1.368 ± 0.015	2.72AlaAsn: 2.72 ± 0.024	2.54AlaPro: 2.54 ± 0.028	2.254AlaGln: 2.254 ± 0.023	3.605AlaArg: 3.605 ± 0.038	4.762AlaSer: 4.762 ± 0.032	3.895AlaThr: 3.895 ± 0.027	3.775AlaVal: 3.775 ± 0.027	0.606AlaTrp: 0.606 ± 0.011	1.508AlaTyr: 1.508 ± 0.017	0.002AlaXaa: 0.002 ± 0.001
Cys	1.099CysAla: 1.099 ± 0.019	0.51CysCys: 0.51 ± 0.011	1.088CysAsp: 1.088 ± 0.021	1.177CysGlu: 1.177 ± 0.024	0.813CysPhe: 0.813 ± 0.014	1.316CysGly: 1.316 ± 0.038	0.513CysHis: 0.513 ± 0.011	1.232CysIle: 1.232 ± 0.028	1.254CysLys: 1.254 ± 0.018	1.853CysLeu: 1.853 ± 0.023	0.485CysMet: 0.485 ± 0.017	1.094CysAsn: 1.094 ± 0.018	1.038CysPro: 1.038 ± 0.036	0.804CysGln: 0.804 ± 0.02	1.196CysArg: 1.196 ± 0.037	1.724CysSer: 1.724 ± 0.036	1.215CysThr: 1.215 ± 0.029	1.28CysVal: 1.28 ± 0.033	0.24CysTrp: 0.24 ± 0.006	0.627CysTyr: 0.627 ± 0.011	0.0CysXaa: 0.0 ± 0.0
Asp	2.844AspAla: 2.844 ± 0.027	1.004AspCys: 1.004 ± 0.025	3.347AspAsp: 3.347 ± 0.034	4.039AspGlu: 4.039 ± 0.037	2.016AspPhe: 2.016 ± 0.019	2.848AspGly: 2.848 ± 0.03	1.072AspHis: 1.072 ± 0.015	3.413AspIle: 3.413 ± 0.033	3.176AspLys: 3.176 ± 0.036	4.519AspLeu: 4.519 ± 0.034	1.097AspMet: 1.097 ± 0.015	2.562AspAsn: 2.562 ± 0.025	2.273AspPro: 2.273 ± 0.034	1.709AspGln: 1.709 ± 0.018	2.805AspArg: 2.805 ± 0.032	4.186AspSer: 4.186 ± 0.038	2.939AspThr: 2.939 ± 0.022	3.44AspVal: 3.44 ± 0.029	0.589AspTrp: 0.589 ± 0.011	1.672AspTyr: 1.672 ± 0.02	0.002AspXaa: 0.002 ± 0.001
Glu	3.794GluAla: 3.794 ± 0.042	1.247GluCys: 1.247 ± 0.039	3.967GluAsp: 3.967 ± 0.04	7.086GluGlu: 7.086 ± 0.126	2.177GluPhe: 2.177 ± 0.021	3.001GluGly: 3.001 ± 0.033	1.438GluHis: 1.438 ± 0.015	4.366GluIle: 4.366 ± 0.04	5.635GluLys: 5.635 ± 0.065	5.694GluLeu: 5.694 ± 0.047	1.558GluMet: 1.558 ± 0.015	4.191GluAsn: 4.191 ± 0.037	2.482GluPro: 2.482 ± 0.036	2.807GluGln: 2.807 ± 0.033	4.279GluArg: 4.279 ± 0.041	4.849GluSer: 4.849 ± 0.044	4.106GluThr: 4.106 ± 0.039	3.575GluVal: 3.575 ± 0.048	0.674GluTrp: 0.674 ± 0.011	1.988GluTyr: 1.988 ± 0.018	0.002GluXaa: 0.002 ± 0.001
Phe	2.094PheAla: 2.094 ± 0.021	0.851PheCys: 0.851 ± 0.013	1.992PheAsp: 1.992 ± 0.02	2.276PheGlu: 2.276 ± 0.024	1.513PhePhe: 1.513 ± 0.018	2.156PheGly: 2.156 ± 0.024	1.034PheHis: 1.034 ± 0.014	2.098PheIle: 2.098 ± 0.022	2.14PheLys: 2.14 ± 0.018	3.64PheLeu: 3.64 ± 0.031	0.764PheMet: 0.764 ± 0.012	1.751PheAsn: 1.751 ± 0.017	1.693PhePro: 1.693 ± 0.017	1.456PheGln: 1.456 ± 0.017	1.999PheArg: 1.999 ± 0.021	2.989PheSer: 2.989 ± 0.023	2.119PheThr: 2.119 ± 0.022	2.412PheVal: 2.412 ± 0.023	0.429PheTrp: 0.429 ± 0.009	1.324PheTyr: 1.324 ± 0.018	0.002PheXaa: 0.002 ± 0.0
Gly	2.971GlyAla: 2.971 ± 0.036	1.058GlyCys: 1.058 ± 0.019	2.616GlyAsp: 2.616 ± 0.029	3.209GlyGlu: 3.209 ± 0.033	2.048GlyPhe: 2.048 ± 0.025	4.184GlyGly: 4.184 ± 0.063	1.309GlyHis: 1.309 ± 0.016	3.173GlyIle: 3.173 ± 0.028	3.516GlyLys: 3.516 ± 0.032	4.273GlyLeu: 4.273 ± 0.041	1.109GlyMet: 1.109 ± 0.013	2.811GlyAsn: 2.811 ± 0.03	2.345GlyPro: 2.345 ± 0.046	2.015GlyGln: 2.015 ± 0.025	3.252GlyArg: 3.252 ± 0.03	4.563GlySer: 4.563 ± 0.042	3.314GlyThr: 3.314 ± 0.039	3.123GlyVal: 3.123 ± 0.039	0.661GlyTrp: 0.661 ± 0.013	1.806GlyTyr: 1.806 ± 0.03	0.002GlyXaa: 0.002 ± 0.001
His	1.358HisAla: 1.358 ± 0.015	0.603HisCys: 0.603 ± 0.011	1.096HisAsp: 1.096 ± 0.013	1.384HisGlu: 1.384 ± 0.017	0.959HisPhe: 0.959 ± 0.011	1.382HisGly: 1.382 ± 0.018	0.983HisHis: 0.983 ± 0.023	1.37HisIle: 1.37 ± 0.015	1.322HisLys: 1.322 ± 0.017	2.317HisLeu: 2.317 ± 0.02	0.54HisMet: 0.54 ± 0.01	1.102HisAsn: 1.102 ± 0.016	1.32HisPro: 1.32 ± 0.016	1.097HisGln: 1.097 ± 0.018	1.557HisArg: 1.557 ± 0.018	1.962HisSer: 1.962 ± 0.02	1.418HisThr: 1.418 ± 0.021	1.556HisVal: 1.556 ± 0.015	0.29HisTrp: 0.29 ± 0.007	0.825HisTyr: 0.825 ± 0.013	0.002HisXaa: 0.002 ± 0.001
Ile	3.58IleAla: 3.58 ± 0.024	1.317IleCys: 1.317 ± 0.026	3.194IleAsp: 3.194 ± 0.027	3.981IleGlu: 3.981 ± 0.039	2.46IlePhe: 2.46 ± 0.029	2.978IleGly: 2.978 ± 0.028	1.509IleHis: 1.509 ± 0.021	3.6IleIle: 3.6 ± 0.038	3.8IleLys: 3.8 ± 0.037	5.649IleLeu: 5.649 ± 0.044	1.21IleMet: 1.21 ± 0.015	3.128IleAsn: 3.128 ± 0.033	2.979IlePro: 2.979 ± 0.025	2.35IleGln: 2.35 ± 0.024	3.067IleArg: 3.067 ± 0.025	4.878IleSer: 4.878 ± 0.034	3.455IleThr: 3.455 ± 0.029	3.734IleVal: 3.734 ± 0.026	0.582IleTrp: 0.582 ± 0.012	1.885IleTyr: 1.885 ± 0.02	0.003IleXaa: 0.003 ± 0.001
Lys	3.207LysAla: 3.207 ± 0.033	1.304LysCys: 1.304 ± 0.022	3.441LysAsp: 3.441 ± 0.039	5.297LysGlu: 5.297 ± 0.06	2.334LysPhe: 2.334 ± 0.023	2.708LysGly: 2.708 ± 0.032	1.607LysHis: 1.607 ± 0.019	4.296LysIle: 4.296 ± 0.037	5.851LysLys: 5.851 ± 0.066	6.054LysLeu: 6.054 ± 0.041	1.541LysMet: 1.541 ± 0.017	3.685LysAsn: 3.685 ± 0.035	2.825LysPro: 2.825 ± 0.062	2.866LysGln: 2.866 ± 0.026	4.274LysArg: 4.274 ± 0.034	4.905LysSer: 4.905 ± 0.05	3.707LysThr: 3.707 ± 0.032	3.607LysVal: 3.607 ± 0.034	0.677LysTrp: 0.677 ± 0.01	2.237LysTyr: 2.237 ± 0.022	0.003LysXaa: 0.003 ± 0.001
Leu	5.598LeuAla: 5.598 ± 0.038	1.861LeuCys: 1.861 ± 0.023	4.527LeuAsp: 4.527 ± 0.031	6.12LeuGlu: 6.12 ± 0.048	3.218LeuPhe: 3.218 ± 0.03	4.337LeuGly: 4.337 ± 0.035	2.399LeuHis: 2.399 ± 0.022	4.844LeuIle: 4.844 ± 0.039	6.141LeuLys: 6.141 ± 0.043	8.693LeuLeu: 8.693 ± 0.072	1.925LeuMet: 1.925 ± 0.019	4.452LeuAsn: 4.452 ± 0.036	4.688LeuPro: 4.688 ± 0.039	4.361LeuGln: 4.361 ± 0.03	5.498LeuArg: 5.498 ± 0.041	7.171LeuSer: 7.171 ± 0.045	5.083LeuThr: 5.083 ± 0.035	4.932LeuVal: 4.932 ± 0.037	0.942LeuTrp: 0.942 ± 0.015	2.746LeuTyr: 2.746 ± 0.025	0.004LeuXaa: 0.004 ± 0.001
Met	1.436MetAla: 1.436 ± 0.015	0.433MetCys: 0.433 ± 0.01	1.206MetAsp: 1.206 ± 0.014	1.615MetGlu: 1.615 ± 0.017	0.824MetPhe: 0.824 ± 0.011	1.036MetGly: 1.036 ± 0.014	0.527MetHis: 0.527 ± 0.01	1.245MetIle: 1.245 ± 0.016	1.589MetLys: 1.589 ± 0.019	1.931MetLeu: 1.931 ± 0.02	0.547MetMet: 0.547 ± 0.011	1.116MetAsn: 1.116 ± 0.016	0.997MetPro: 0.997 ± 0.013	0.996MetGln: 0.996 ± 0.014	1.169MetArg: 1.169 ± 0.015	1.691MetSer: 1.691 ± 0.017	1.209MetThr: 1.209 ± 0.015	1.178MetVal: 1.178 ± 0.016	0.222MetTrp: 0.222 ± 0.005	0.804MetTyr: 0.804 ± 0.017	0.001MetXaa: 0.001 ± 0.0
Asn	2.914AsnAla: 2.914 ± 0.03	1.018AsnCys: 1.018 ± 0.015	2.764AsnAsp: 2.764 ± 0.023	3.611AsnGlu: 3.611 ± 0.036	2.063AsnPhe: 2.063 ± 0.021	2.854AsnGly: 2.854 ± 0.032	1.157AsnHis: 1.157 ± 0.017	3.609AsnIle: 3.609 ± 0.036	3.378AsnLys: 3.378 ± 0.028	4.634AsnLeu: 4.634 ± 0.036	1.133AsnMet: 1.133 ± 0.015	3.326AsnAsn: 3.326 ± 0.039	2.253AsnPro: 2.253 ± 0.031	1.969AsnGln: 1.969 ± 0.031	2.618AsnArg: 2.618 ± 0.023	4.339AsnSer: 4.339 ± 0.042	3.023AsnThr: 3.023 ± 0.03	3.639AsnVal: 3.639 ± 0.029	0.551AsnTrp: 0.551 ± 0.011	1.723AsnTyr: 1.723 ± 0.02	0.003AsnXaa: 0.003 ± 0.001
Pro	2.946ProAla: 2.946 ± 0.039	0.881ProCys: 0.881 ± 0.05	2.32ProAsp: 2.32 ± 0.024	3.145ProGlu: 3.145 ± 0.046	1.61ProPhe: 1.61 ± 0.02	2.917ProGly: 2.917 ± 0.102	1.148ProHis: 1.148 ± 0.017	2.623ProIle: 2.623 ± 0.022	2.679ProLys: 2.679 ± 0.032	4.089ProLeu: 4.089 ± 0.027	0.955ProMet: 0.955 ± 0.015	2.217ProAsn: 2.217 ± 0.028	4.143ProPro: 4.143 ± 0.072	1.997ProGln: 1.997 ± 0.027	2.875ProArg: 2.875 ± 0.027	4.329ProSer: 4.329 ± 0.047	3.108ProThr: 3.108 ± 0.032	3.094ProVal: 3.094 ± 0.036	0.502ProTrp: 0.502 ± 0.01	1.424ProTyr: 1.424 ± 0.021	0.003ProXaa: 0.003 ± 0.001
Gln	2.289GlnAla: 2.289 ± 0.024	0.826GlnCys: 0.826 ± 0.022	1.858GlnAsp: 1.858 ± 0.019	2.9GlnGlu: 2.9 ± 0.038	1.435GlnPhe: 1.435 ± 0.019	1.823GlnGly: 1.823 ± 0.024	1.168GlnHis: 1.168 ± 0.018	2.419GlnIle: 2.419 ± 0.022	2.722GlnLys: 2.722 ± 0.029	3.893GlnLeu: 3.893 ± 0.028	0.949GlnMet: 0.949 ± 0.015	2.348GlnAsn: 2.348 ± 0.027	2.029GlnPro: 2.029 ± 0.038	3.152GlnGln: 3.152 ± 0.074	2.7GlnArg: 2.7 ± 0.021	3.176GlnSer: 3.176 ± 0.029	2.468GlnThr: 2.468 ± 0.024	2.216GlnVal: 2.216 ± 0.024	0.458GlnTrp: 0.458 ± 0.008	1.257GlnTyr: 1.257 ± 0.016	0.003GlnXaa: 0.003 ± 0.001
Arg	3.224ArgAla: 3.224 ± 0.028	1.212ArgCys: 1.212 ± 0.021	3.063ArgAsp: 3.063 ± 0.03	3.952ArgGlu: 3.952 ± 0.031	2.088ArgPhe: 2.088 ± 0.022	3.228ArgGly: 3.228 ± 0.037	1.577ArgHis: 1.577 ± 0.018	3.302ArgIle: 3.302 ± 0.029	4.423ArgLys: 4.423 ± 0.033	5.13ArgLeu: 5.13 ± 0.04	1.273ArgMet: 1.273 ± 0.015	3.252ArgAsn: 3.252 ± 0.034	2.616ArgPro: 2.616 ± 0.043	2.478ArgGln: 2.478 ± 0.025	4.749ArgArg: 4.749 ± 0.048	4.724ArgSer: 4.724 ± 0.046	3.178ArgThr: 3.178 ± 0.027	3.248ArgVal: 3.248 ± 0.024	0.664ArgTrp: 0.664 ± 0.012	1.875ArgTyr: 1.875 ± 0.018	0.002ArgXaa: 0.002 ± 0.0
Ser	4.452SerAla: 4.452 ± 0.033	1.601SerCys: 1.601 ± 0.036	4.139SerAsp: 4.139 ± 0.035	4.945SerGlu: 4.945 ± 0.043	2.898SerPhe: 2.898 ± 0.023	4.681SerGly: 4.681 ± 0.034	1.878SerHis: 1.878 ± 0.018	4.541SerIle: 4.541 ± 0.034	4.971SerLys: 4.971 ± 0.044	7.169SerLeu: 7.169 ± 0.045	1.73SerMet: 1.73 ± 0.017	4.531SerAsn: 4.531 ± 0.04	4.478SerPro: 4.478 ± 0.052	3.31SerGln: 3.31 ± 0.036	4.762SerArg: 4.762 ± 0.044	8.803SerSer: 8.803 ± 0.077	5.488SerThr: 5.488 ± 0.041	4.945SerVal: 4.945 ± 0.035	0.863SerTrp: 0.863 ± 0.012	2.328SerTyr: 2.328 ± 0.022	0.003SerXaa: 0.003 ± 0.001
Thr	3.646ThrAla: 3.646 ± 0.027	1.265ThrCys: 1.265 ± 0.031	2.933ThrAsp: 2.933 ± 0.028	3.922ThrGlu: 3.922 ± 0.046	2.252ThrPhe: 2.252 ± 0.019	3.265ThrGly: 3.265 ± 0.03	1.266ThrHis: 1.266 ± 0.014	3.658ThrIle: 3.658 ± 0.028	3.698ThrLys: 3.698 ± 0.03	5.371ThrLeu: 5.371 ± 0.028	1.358ThrMet: 1.358 ± 0.014	3.098ThrAsn: 3.098 ± 0.027	3.282ThrPro: 3.282 ± 0.038	2.154ThrGln: 2.154 ± 0.021	3.129ThrArg: 3.129 ± 0.027	5.4ThrSer: 5.4 ± 0.05	4.373ThrThr: 4.373 ± 0.05	3.943ThrVal: 3.943 ± 0.034	0.655ThrTrp: 0.655 ± 0.015	1.751ThrTyr: 1.751 ± 0.023	0.003ThrXaa: 0.003 ± 0.001
Val	3.972ValAla: 3.972 ± 0.033	1.445ValCys: 1.445 ± 0.028	3.135ValAsp: 3.135 ± 0.021	3.989ValGlu: 3.989 ± 0.049	2.184ValPhe: 2.184 ± 0.023	3.079ValGly: 3.079 ± 0.038	1.47ValHis: 1.47 ± 0.016	3.483ValIle: 3.483 ± 0.029	3.782ValLys: 3.782 ± 0.036	5.349ValLeu: 5.349 ± 0.037	1.27ValMet: 1.27 ± 0.016	2.875ValAsn: 2.875 ± 0.026	3.171ValPro: 3.171 ± 0.035	2.591ValGln: 2.591 ± 0.027	3.231ValArg: 3.231 ± 0.025	4.82ValSer: 4.82 ± 0.03	3.916ValThr: 3.916 ± 0.047	3.852ValVal: 3.852 ± 0.035	0.668ValTrp: 0.668 ± 0.012	1.795ValTyr: 1.795 ± 0.021	0.003ValXaa: 0.003 ± 0.001
Trp	0.526TrpAla: 0.526 ± 0.01	0.221TrpCys: 0.221 ± 0.006	0.547TrpAsp: 0.547 ± 0.012	0.624TrpGlu: 0.624 ± 0.011	0.445TrpPhe: 0.445 ± 0.01	0.522TrpGly: 0.522 ± 0.011	0.279TrpHis: 0.279 ± 0.007	0.688TrpIle: 0.688 ± 0.013	0.829TrpLys: 0.829 ± 0.013	1.085TrpLeu: 1.085 ± 0.014	0.273TrpMet: 0.273 ± 0.007	0.644TrpAsn: 0.644 ± 0.011	0.431TrpPro: 0.431 ± 0.009	0.458TrpGln: 0.458 ± 0.008	0.697TrpArg: 0.697 ± 0.01	0.846TrpSer: 0.846 ± 0.018	0.607TrpThr: 0.607 ± 0.011	0.537TrpVal: 0.537 ± 0.012	0.177TrpTrp: 0.177 ± 0.006	0.372TrpTyr: 0.372 ± 0.009	0.0TrpXaa: 0.0 ± 0.0
Tyr	1.686TyrAla: 1.686 ± 0.016	0.734TyrCys: 0.734 ± 0.013	1.62TyrAsp: 1.62 ± 0.02	1.924TyrGlu: 1.924 ± 0.02	1.348TyrPhe: 1.348 ± 0.016	1.709TyrGly: 1.709 ± 0.022	0.854TyrHis: 0.854 ± 0.012	1.926TyrIle: 1.926 ± 0.027	1.926TyrLys: 1.926 ± 0.022	2.85TyrLeu: 2.85 ± 0.023	0.682TyrMet: 0.682 ± 0.01	1.674TyrAsn: 1.674 ± 0.019	1.418TyrPro: 1.418 ± 0.024	1.265TyrGln: 1.265 ± 0.017	1.791TyrArg: 1.791 ± 0.018	2.393TyrSer: 2.393 ± 0.022	1.8TyrThr: 1.8 ± 0.021	1.99TyrVal: 1.99 ± 0.027	0.358TyrTrp: 0.358 ± 0.008	1.211TyrTyr: 1.211 ± 0.016	0.002TyrXaa: 0.002 ± 0.0
Xaa	0.002XaaAla: 0.002 ± 0.001	0.002XaaCys: 0.002 ± 0.0	0.001XaaAsp: 0.001 ± 0.0	0.003XaaGlu: 0.003 ± 0.001	0.002XaaPhe: 0.002 ± 0.001	0.002XaaGly: 0.002 ± 0.001	0.001XaaHis: 0.001 ± 0.0	0.004XaaIle: 0.004 ± 0.001	0.004XaaLys: 0.004 ± 0.001	0.004XaaLeu: 0.004 ± 0.001	0.002XaaMet: 0.002 ± 0.001	0.003XaaAsn: 0.003 ± 0.001	0.002XaaPro: 0.002 ± 0.001	0.001XaaGln: 0.001 ± 0.0	0.002XaaArg: 0.002 ± 0.001	0.003XaaSer: 0.003 ± 0.001	0.003XaaThr: 0.003 ± 0.001	0.002XaaVal: 0.002 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.001XaaTyr: 0.001 ± 0.0	0.939XaaXaa: 0.939 ± 0.13

Statistics based on 14253 proteins (6552381 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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