Caloramator quimbayensis
Average proteome isoelectric point is 6.67
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2996 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1T4XLY1|A0A1T4XLY1_9CLOT 3'-5' exoribonuclease OS=Caloramator quimbayensis OX=1147123 GN=SAMN05443428_11090 PE=4 SV=1
MM1 pKa = 7.62 IKK3 pKa = 10.2 HH4 pKa = 6.09 KK5 pKa = 10.55 EE6 pKa = 3.69 ILNFLEE12 pKa = 4.42 NEE14 pKa = 4.04 GLEE17 pKa = 4.39 EE18 pKa = 4.32 IDD20 pKa = 3.68 EE21 pKa = 4.21 IEE23 pKa = 4.14 YY24 pKa = 11.19 NKK26 pKa = 10.88 DD27 pKa = 2.5 IFVYY31 pKa = 10.61 NFFYY35 pKa = 10.62 TFDD38 pKa = 3.59 EE39 pKa = 4.71 AEE41 pKa = 3.8 IEE43 pKa = 4.17 AAKK46 pKa = 10.39 EE47 pKa = 3.8 YY48 pKa = 11.33 ANEE51 pKa = 4.06 NCDD54 pKa = 3.69 AEE56 pKa = 4.48 STEE59 pKa = 4.86 DD60 pKa = 3.06 EE61 pKa = 4.46 WNEE64 pKa = 3.94 EE65 pKa = 4.01 FYY67 pKa = 11.2 LPYY70 pKa = 9.91 LTDD73 pKa = 3.27 IAADD77 pKa = 3.63 NVRR80 pKa = 11.84 DD81 pKa = 3.88 IVDD84 pKa = 4.97 DD85 pKa = 3.43 ICEE88 pKa = 4.01 EE89 pKa = 3.99 FGLFGEE95 pKa = 5.08 FVAYY99 pKa = 9.79 EE100 pKa = 4.0 MDD102 pKa = 3.28 MGSSEE107 pKa = 4.13 RR108 pKa = 11.84 CEE110 pKa = 3.97 FTVVFAKK117 pKa = 10.56 EE118 pKa = 4.26 GIEE121 pKa = 3.92 FDD123 pKa = 3.34 IDD125 pKa = 3.82 EE126 pKa = 4.43 IMEE129 pKa = 4.27 KK130 pKa = 11.03 LEE132 pKa = 3.99 LL133 pKa = 4.55
Molecular weight: 15.74 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.754
IPC2_protein 3.706
IPC_protein 3.656
Toseland 3.478
ProMoST 3.783
Dawson 3.617
Bjellqvist 3.77
Wikipedia 3.503
Rodwell 3.49
Grimsley 3.389
Solomon 3.605
Lehninger 3.554
Nozaki 3.732
DTASelect 3.859
Thurlkill 3.503
EMBOSS 3.516
Sillero 3.77
Patrickios 0.985
IPC_peptide 3.605
IPC2_peptide 3.757
IPC2.peptide.svr19 3.712
Protein with the highest isoelectric point:
>tr|A0A1T4Y8E7|A0A1T4Y8E7_9CLOT LysR substrate binding domain-containing protein OS=Caloramator quimbayensis OX=1147123 GN=SAMN05443428_1274 PE=4 SV=1
MM1 pKa = 7.45 LRR3 pKa = 11.84 TYY5 pKa = 9.8 QPKK8 pKa = 9.46 KK9 pKa = 8.28 RR10 pKa = 11.84 QRR12 pKa = 11.84 KK13 pKa = 8.39 KK14 pKa = 8.49 EE15 pKa = 3.5 HH16 pKa = 6.11 GFRR19 pKa = 11.84 KK20 pKa = 9.7 RR21 pKa = 11.84 MKK23 pKa = 8.35 TRR25 pKa = 11.84 SGRR28 pKa = 11.84 LVLQRR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 RR36 pKa = 11.84 KK37 pKa = 9.44 GRR39 pKa = 11.84 KK40 pKa = 9.05 KK41 pKa = 9.62 LTAA44 pKa = 4.2
Molecular weight: 5.55 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.359
IPC2_protein 10.921
IPC_protein 12.325
Toseland 12.486
ProMoST 12.983
Dawson 12.501
Bjellqvist 12.486
Wikipedia 12.969
Rodwell 12.281
Grimsley 12.53
Solomon 12.983
Lehninger 12.881
Nozaki 12.486
DTASelect 12.486
Thurlkill 12.486
EMBOSS 12.983
Sillero 12.486
Patrickios 12.003
IPC_peptide 12.983
IPC2_peptide 11.974
IPC2.peptide.svr19 9.043
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2996
0
2996
921638
25
2731
307.6
34.78
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.854 ± 0.056
1.268 ± 0.021
5.669 ± 0.034
7.181 ± 0.051
4.497 ± 0.032
6.369 ± 0.049
1.281 ± 0.016
10.42 ± 0.048
9.202 ± 0.05
8.835 ± 0.04
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.581 ± 0.021
5.956 ± 0.045
2.986 ± 0.024
2.272 ± 0.024
3.576 ± 0.035
6.204 ± 0.045
4.539 ± 0.034
6.295 ± 0.04
0.655 ± 0.014
4.359 ± 0.037
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here