Caloramator quimbayensis 
Average proteome isoelectric point is 6.67 
Get precalculated fractions of proteins 
 
  
    Acidic  
 
  
    pI < 6.8  
 
  
    6.8-7.4  
 
  
    pI > 7.4  
 
  
    Basic  
    
 
  
    All  
 
Note: above files contain also dissociation constants (pKa) 
Virtual 2D-PAGE plot for 2996 proteins (isoelectric point calculated using IPC2_protein) 
 
Get csv file with sequences according to given criteria: 
* You can choose from 21 different methods for calculating isoelectric point 
 Summary statistics related to proteome-wise predictions 
Protein with the lowest isoelectric point: 
>tr|A0A1T4XLY1|A0A1T4XLY1_9CLOT 3'-5' exoribonuclease OS=Caloramator quimbayensis OX=1147123 GN=SAMN05443428_11090 PE=4 SV=1MM1 pKa = 7.62  IKK3 pKa = 10.2  HH4 pKa = 6.09  KK5 pKa = 10.55  EE6 pKa = 3.69  ILNFLEE12 pKa = 4.42  NEE14 pKa = 4.04  GLEE17 pKa = 4.39  EE18 pKa = 4.32  IDD20 pKa = 3.68  EE21 pKa = 4.21  IEE23 pKa = 4.14  YY24 pKa = 11.19  NKK26 pKa = 10.88  DD27 pKa = 2.5  IFVYY31 pKa = 10.61  NFFYY35 pKa = 10.62  TFDD38 pKa = 3.59  EE39 pKa = 4.71  AEE41 pKa = 3.8  IEE43 pKa = 4.17  AAKK46 pKa = 10.39  EE47 pKa = 3.8  YY48 pKa = 11.33  ANEE51 pKa = 4.06  NCDD54 pKa = 3.69  AEE56 pKa = 4.48  STEE59 pKa = 4.86  DD60 pKa = 3.06  EE61 pKa = 4.46  WNEE64 pKa = 3.94  EE65 pKa = 4.01  FYY67 pKa = 11.2  LPYY70 pKa = 9.91  LTDD73 pKa = 3.27  IAADD77 pKa = 3.63  NVRR80 pKa = 11.84  DD81 pKa = 3.88  IVDD84 pKa = 4.97  DD85 pKa = 3.43  ICEE88 pKa = 4.01  EE89 pKa = 3.99  FGLFGEE95 pKa = 5.08  FVAYY99 pKa = 9.79  EE100 pKa = 4.0  MDD102 pKa = 3.28  MGSSEE107 pKa = 4.13  RR108 pKa = 11.84  CEE110 pKa = 3.97  FTVVFAKK117 pKa = 10.56  EE118 pKa = 4.26  GIEE121 pKa = 3.92  FDD123 pKa = 3.34  IDD125 pKa = 3.82  EE126 pKa = 4.43  IMEE129 pKa = 4.27  KK130 pKa = 11.03  LEE132 pKa = 3.99  LL133 pKa = 4.55  
 15.74 kDa
Isoelectric point according different methods: 
IPC2.protein.svr19  3.754 
IPC2_protein 3.706 
IPC_protein 3.656 
Toseland    3.478 
ProMoST     3.783 
Dawson      3.617 
Bjellqvist  3.77 
Wikipedia   3.503 
Rodwell     3.49 
Grimsley    3.389 
Solomon     3.605 
Lehninger   3.554 
Nozaki      3.732 
DTASelect   3.859 
Thurlkill   3.503 
EMBOSS      3.516 
Sillero     3.77 
Patrickios  0.985 
IPC_peptide 3.605 
IPC2_peptide  3.757 
IPC2.peptide.svr19  3.712 
 Protein with the highest isoelectric point: 
>tr|A0A1T4Y8E7|A0A1T4Y8E7_9CLOT LysR substrate binding domain-containing protein OS=Caloramator quimbayensis OX=1147123 GN=SAMN05443428_1274 PE=4 SV=1MM1 pKa = 7.45  LRR3 pKa = 11.84  TYY5 pKa = 9.8  QPKK8 pKa = 9.46  KK9 pKa = 8.28  RR10 pKa = 11.84  QRR12 pKa = 11.84  KK13 pKa = 8.39  KK14 pKa = 8.49  EE15 pKa = 3.5  HH16 pKa = 6.11  GFRR19 pKa = 11.84  KK20 pKa = 9.7  RR21 pKa = 11.84  MKK23 pKa = 8.35  TRR25 pKa = 11.84  SGRR28 pKa = 11.84  LVLQRR33 pKa = 11.84  RR34 pKa = 11.84  RR35 pKa = 11.84  RR36 pKa = 11.84  KK37 pKa = 9.44  GRR39 pKa = 11.84  KK40 pKa = 9.05  KK41 pKa = 9.62  LTAA44 pKa = 4.2  
 5.55 kDa
Isoelectric point according different methods: 
IPC2.protein.svr19  9.359 
IPC2_protein 10.921 
IPC_protein 12.325 
Toseland    12.486 
ProMoST     12.983 
Dawson      12.501 
Bjellqvist  12.486 
Wikipedia   12.969 
Rodwell     12.281 
Grimsley    12.53 
Solomon     12.983 
Lehninger   12.881 
Nozaki      12.486 
DTASelect   12.486 
Thurlkill   12.486 
EMBOSS      12.983 
Sillero     12.486 
Patrickios  12.003 
IPC_peptide 12.983 
IPC2_peptide  11.974 
IPC2.peptide.svr19  9.043 
 Peptides (in silico  digests for buttom-up proteomics) 
Below you can find 
in silico  digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
 
  
    Try  
 
  
    ChTry  
 
  
    ArgC  
 
  
    LysN  
 
  
    TryLysC  
 
  
    Try  
 
  
    ChTry  
 
  
    ArgC  
 
  
    LysN  
 
  
    TryLysC  
 
  
    Try  
 
  
    ChTry  
 
  
    ArgC  
 
  
    LysN  
 
  
    TryLysC  
 
  
    Try  
 
  
    ChTry  
 
  
    ArgC  
 
  
    LysN  
 
  
    TryLysC  
 
  
    Try  
 
  
    ChTry  
 
  
    ArgC  
 
  
    LysN  
 
  
    TryLysC  
   
  
General Statistics 
    
      
        Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
 
     
    
      
        2996 
0
2996 
921638
25
2731
307.6
34.78
          
     
  
 
    Amino acid frequency 
  
  
    
      
        Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
 
     
    
                 
        5.854 ± 0.056
1.268 ± 0.021
5.669 ± 0.034
7.181 ± 0.051
4.497 ± 0.032
6.369 ± 0.049
1.281 ± 0.016
10.42 ± 0.048
9.202 ± 0.05
8.835 ± 0.04
          
     
  
  
  
      
          
        Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
 
     
    
                 
        2.581 ± 0.021
5.956 ± 0.045
2.986 ± 0.024
2.272 ± 0.024
3.576 ± 0.035
6.204 ± 0.045
4.539 ± 0.034
6.295 ± 0.04
0.655 ± 0.014
4.359 ± 0.037
          
     
  
  
  
Note:  For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein levelMost of the basic statistics you can see at this page can be downloaded from this CSV file  
For dipeptide frequency statistics click here