Methanococcus aeolicus (strain ATCC BAA-1280 / DSM 17508 / OCM 812 / Nankai-3)

Taxonomy: cellular organisms; Archaea; Euryarchaeota; Methanomada group; Methanococci; Methanococcales; Methanococcaceae; Methanococcus; Methanococcus aeolicus

Average proteome isoelectric point is 6.49

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1490 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A6UX03|A6UX03_META3 DNA topoisomerase type IA zn finger domain protein OS=Methanococcus aeolicus (strain ATCC BAA-1280 / DSM 17508 / OCM 812 / Nankai-3) OX=419665 GN=Maeo_1449 PE=4 SV=1
MM1 pKa = 7.66VVEE4 pKa = 4.93IDD6 pKa = 3.73YY7 pKa = 10.94NNCDD11 pKa = 4.14GIDD14 pKa = 3.59CSGCKK19 pKa = 9.47DD20 pKa = 3.38ACPTDD25 pKa = 3.44VFGVEE30 pKa = 3.97GSKK33 pKa = 10.29IVVSNADD40 pKa = 3.12EE41 pKa = 4.5CTFCMMCEE49 pKa = 4.14DD50 pKa = 5.15LCPADD55 pKa = 3.52AVKK58 pKa = 10.84VKK60 pKa = 10.72NN61 pKa = 3.78

Molecular weight: 6.52 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A6UVS6|A6UVS6_META3 Hydrogenase accessory protein HypB OS=Methanococcus aeolicus (strain ATCC BAA-1280 / DSM 17508 / OCM 812 / Nankai-3) OX=419665 GN=Maeo_1020 PE=4 SV=1
MM1 pKa = 7.73SSIKK5 pKa = 10.03PAAKK9 pKa = 10.2KK10 pKa = 10.33LRR12 pKa = 11.84LAKK15 pKa = 10.27AGKK18 pKa = 7.7QNRR21 pKa = 11.84RR22 pKa = 11.84VPMFVIAKK30 pKa = 8.46TGGKK34 pKa = 9.49VRR36 pKa = 11.84THH38 pKa = 4.24TKK40 pKa = 7.39MRR42 pKa = 11.84NWRR45 pKa = 11.84RR46 pKa = 11.84NTLKK50 pKa = 10.77KK51 pKa = 10.76

Molecular weight: 5.89 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1490

0

1490

439175

30

2064

294.7

33.2

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.4 ± 0.062

1.423 ± 0.032

5.635 ± 0.047

7.487 ± 0.065

3.665 ± 0.046

6.678 ± 0.051

1.598 ± 0.023

10.825 ± 0.068

9.604 ± 0.063

8.685 ± 0.065

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.628 ± 0.031

7.058 ± 0.095

3.467 ± 0.035

1.777 ± 0.026

2.965 ± 0.04

5.428 ± 0.051

4.832 ± 0.046

6.085 ± 0.052

0.615 ± 0.02

4.146 ± 0.054
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski