Amino acid dipepetide frequency for Methanococcus aeolicus (strain ATCC BAA-1280 / DSM 17508 / OCM 812 / Nankai-3)

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
2.2AlaAla: 2.2 ± 0.118
0.836AlaCys: 0.836 ± 0.063
2.803AlaAsp: 2.803 ± 0.085
3.609AlaGlu: 3.609 ± 0.092
1.972AlaPhe: 1.972 ± 0.068
3.805AlaGly: 3.805 ± 0.122
0.995AlaHis: 0.995 ± 0.048
5.829AlaIle: 5.829 ± 0.15
5.057AlaLys: 5.057 ± 0.099
5.376AlaLeu: 5.376 ± 0.122
1.583AlaMet: 1.583 ± 0.052
2.687AlaAsn: 2.687 ± 0.076
2.464AlaPro: 2.464 ± 0.075
1.052AlaGln: 1.052 ± 0.053
1.674AlaArg: 1.674 ± 0.063
3.024AlaSer: 3.024 ± 0.092
2.862AlaThr: 2.862 ± 0.084
3.907AlaVal: 3.907 ± 0.125
0.312AlaTrp: 0.312 ± 0.028
1.951AlaTyr: 1.951 ± 0.077
0.0AlaXaa: 0.0 ± 0.0
Cys
0.811CysAla: 0.811 ± 0.051
0.246CysCys: 0.246 ± 0.029
0.924CysAsp: 0.924 ± 0.045
0.897CysGlu: 0.897 ± 0.046
0.38CysPhe: 0.38 ± 0.029
1.737CysGly: 1.737 ± 0.069
0.335CysHis: 0.335 ± 0.026
1.25CysIle: 1.25 ± 0.056
1.05CysLys: 1.05 ± 0.054
0.838CysLeu: 0.838 ± 0.049
0.387CysMet: 0.387 ± 0.03
0.827CysAsn: 0.827 ± 0.051
1.095CysPro: 1.095 ± 0.085
0.239CysGln: 0.239 ± 0.024
0.357CysArg: 0.357 ± 0.029
0.811CysSer: 0.811 ± 0.047
0.531CysThr: 0.531 ± 0.04
0.94CysVal: 0.94 ± 0.068
0.075CysTrp: 0.075 ± 0.013
0.501CysTyr: 0.501 ± 0.038
0.0CysXaa: 0.0 ± 0.0
Asp
3.488AspAla: 3.488 ± 0.099
0.722AspCys: 0.722 ± 0.045
2.892AspAsp: 2.892 ± 0.096
4.524AspGlu: 4.524 ± 0.12
2.32AspPhe: 2.32 ± 0.078
3.705AspGly: 3.705 ± 0.091
0.587AspHis: 0.587 ± 0.034
7.642AspIle: 7.642 ± 0.116
5.419AspLys: 5.419 ± 0.126
4.925AspLeu: 4.925 ± 0.106
1.337AspMet: 1.337 ± 0.061
3.873AspAsn: 3.873 ± 0.115
1.389AspPro: 1.389 ± 0.06
0.428AspGln: 0.428 ± 0.033
1.635AspArg: 1.635 ± 0.069
2.489AspSer: 2.489 ± 0.082
2.518AspThr: 2.518 ± 0.067
3.45AspVal: 3.45 ± 0.109
0.41AspTrp: 0.41 ± 0.03
2.598AspTyr: 2.598 ± 0.081
0.0AspXaa: 0.0 ± 0.0
Glu
3.361GluAla: 3.361 ± 0.097
0.858GluCys: 0.858 ± 0.055
3.944GluAsp: 3.944 ± 0.101
6.879GluGlu: 6.879 ± 0.176
2.962GluPhe: 2.962 ± 0.09
4.224GluGly: 4.224 ± 0.104
1.191GluHis: 1.191 ± 0.057
8.181GluIle: 8.181 ± 0.157
7.746GluLys: 7.746 ± 0.162
8.304GluLeu: 8.304 ± 0.156
1.954GluMet: 1.954 ± 0.075
5.752GluAsn: 5.752 ± 0.131
1.671GluPro: 1.671 ± 0.06
1.694GluGln: 1.694 ± 0.069
2.047GluArg: 2.047 ± 0.07
2.587GluSer: 2.587 ± 0.08
3.16GluThr: 3.16 ± 0.088
4.483GluVal: 4.483 ± 0.117
0.46GluTrp: 0.46 ± 0.037
3.409GluTyr: 3.409 ± 0.089
0.0GluXaa: 0.0 ± 0.0
Phe
2.042PheAla: 2.042 ± 0.069
0.542PheCys: 0.542 ± 0.036
2.313PheAsp: 2.313 ± 0.071
2.924PheGlu: 2.924 ± 0.084
1.2PhePhe: 1.2 ± 0.06
3.012PheGly: 3.012 ± 0.096
0.49PheHis: 0.49 ± 0.031
3.586PheIle: 3.586 ± 0.108
3.518PheLys: 3.518 ± 0.092
3.108PheLeu: 3.108 ± 0.084
0.99PheMet: 0.99 ± 0.052
2.345PheAsn: 2.345 ± 0.078
1.129PhePro: 1.129 ± 0.056
0.519PheGln: 0.519 ± 0.033
0.988PheArg: 0.988 ± 0.053
2.131PheSer: 2.131 ± 0.076
1.432PheThr: 1.432 ± 0.057
2.726PheVal: 2.726 ± 0.094
0.262PheTrp: 0.262 ± 0.028
1.389PheTyr: 1.389 ± 0.058
0.0PheXaa: 0.0 ± 0.0
Gly
5.123GlyAla: 5.123 ± 0.125
1.289GlyCys: 1.289 ± 0.064
3.798GlyAsp: 3.798 ± 0.085
4.272GlyGlu: 4.272 ± 0.089
2.764GlyPhe: 2.764 ± 0.097
4.905GlyGly: 4.905 ± 0.123
1.273GlyHis: 1.273 ± 0.057
7.102GlyIle: 7.102 ± 0.139
5.581GlyLys: 5.581 ± 0.125
5.426GlyLeu: 5.426 ± 0.133
1.794GlyMet: 1.794 ± 0.067
3.338GlyAsn: 3.338 ± 0.095
1.305GlyPro: 1.305 ± 0.061
1.25GlyGln: 1.25 ± 0.065
2.197GlyArg: 2.197 ± 0.075
3.431GlySer: 3.431 ± 0.095
3.725GlyThr: 3.725 ± 0.109
4.763GlyVal: 4.763 ± 0.117
0.49GlyTrp: 0.49 ± 0.035
2.956GlyTyr: 2.956 ± 0.099
0.0GlyXaa: 0.0 ± 0.0
His
0.934HisAla: 0.934 ± 0.047
0.348HisCys: 0.348 ± 0.03
0.77HisAsp: 0.77 ± 0.051
0.788HisGlu: 0.788 ± 0.045
0.54HisPhe: 0.54 ± 0.033
1.07HisGly: 1.07 ± 0.051
0.433HisHis: 0.433 ± 0.038
1.664HisIle: 1.664 ± 0.064
1.332HisLys: 1.332 ± 0.053
1.357HisLeu: 1.357 ± 0.069
0.353HisMet: 0.353 ± 0.034
1.225HisAsn: 1.225 ± 0.058
0.977HisPro: 0.977 ± 0.045
0.355HisGln: 0.355 ± 0.033
0.544HisArg: 0.544 ± 0.04
0.981HisSer: 0.981 ± 0.047
0.868HisThr: 0.868 ± 0.046
0.66HisVal: 0.66 ± 0.037
0.089HisTrp: 0.089 ± 0.015
0.69HisTyr: 0.69 ± 0.034
0.0HisXaa: 0.0 ± 0.0
Ile
5.547IleAla: 5.547 ± 0.119
1.446IleCys: 1.446 ± 0.056
6.535IleAsp: 6.535 ± 0.114
7.701IleGlu: 7.701 ± 0.146
3.837IlePhe: 3.837 ± 0.103
7.004IleGly: 7.004 ± 0.147
1.601IleHis: 1.601 ± 0.063
11.522IleIle: 11.522 ± 0.207
10.21IleLys: 10.21 ± 0.158
9.308IleLeu: 9.308 ± 0.175
2.589IleMet: 2.589 ± 0.083
7.601IleAsn: 7.601 ± 0.181
4.208IlePro: 4.208 ± 0.106
1.687IleGln: 1.687 ± 0.06
2.992IleArg: 2.992 ± 0.086
6.785IleSer: 6.785 ± 0.139
6.191IleThr: 6.191 ± 0.165
6.815IleVal: 6.815 ± 0.114
0.528IleTrp: 0.528 ± 0.04
4.14IleTyr: 4.14 ± 0.105
0.0IleXaa: 0.0 ± 0.0
Lys
3.86LysAla: 3.86 ± 0.113
1.195LysCys: 1.195 ± 0.066
5.504LysAsp: 5.504 ± 0.123
9.365LysGlu: 9.365 ± 0.174
2.974LysPhe: 2.974 ± 0.087
4.9LysGly: 4.9 ± 0.121
1.366LysHis: 1.366 ± 0.058
11.392LysIle: 11.392 ± 0.161
9.611LysLys: 9.611 ± 0.175
8.302LysLeu: 8.302 ± 0.144
2.757LysMet: 2.757 ± 0.08
8.227LysAsn: 8.227 ± 0.19
2.644LysPro: 2.644 ± 0.076
1.769LysGln: 1.769 ± 0.069
2.926LysArg: 2.926 ± 0.093
4.413LysSer: 4.413 ± 0.103
4.716LysThr: 4.716 ± 0.104
4.672LysVal: 4.672 ± 0.119
0.558LysTrp: 0.558 ± 0.035
4.891LysTyr: 4.891 ± 0.137
0.0LysXaa: 0.0 ± 0.0
Leu
4.6LeuAla: 4.6 ± 0.133
1.33LeuCys: 1.33 ± 0.067
4.741LeuAsp: 4.741 ± 0.096
5.56LeuGlu: 5.56 ± 0.113
3.486LeuPhe: 3.486 ± 0.106
5.51LeuGly: 5.51 ± 0.124
1.227LeuHis: 1.227 ± 0.055
8.448LeuIle: 8.448 ± 0.157
10.169LeuLys: 10.169 ± 0.165
7.371LeuLeu: 7.371 ± 0.168
2.541LeuMet: 2.541 ± 0.085
7.229LeuAsn: 7.229 ± 0.155
3.06LeuPro: 3.06 ± 0.083
1.441LeuGln: 1.441 ± 0.058
2.912LeuArg: 2.912 ± 0.098
5.697LeuSer: 5.697 ± 0.133
4.023LeuThr: 4.023 ± 0.099
4.037LeuVal: 4.037 ± 0.09
0.492LeuTrp: 0.492 ± 0.031
2.971LeuTyr: 2.971 ± 0.084
0.0LeuXaa: 0.0 ± 0.0
Met
2.056MetAla: 2.056 ± 0.071
0.276MetCys: 0.276 ± 0.026
2.122MetAsp: 2.122 ± 0.063
2.411MetGlu: 2.411 ± 0.08
0.804MetPhe: 0.804 ± 0.04
2.423MetGly: 2.423 ± 0.076
0.428MetHis: 0.428 ± 0.029
2.291MetIle: 2.291 ± 0.068
2.179MetLys: 2.179 ± 0.073
1.767MetLeu: 1.767 ± 0.066
0.71MetMet: 0.71 ± 0.043
1.478MetAsn: 1.478 ± 0.054
1.038MetPro: 1.038 ± 0.051
0.478MetGln: 0.478 ± 0.034
0.669MetArg: 0.669 ± 0.035
1.284MetSer: 1.284 ± 0.053
1.125MetThr: 1.125 ± 0.05
1.938MetVal: 1.938 ± 0.066
0.139MetTrp: 0.139 ± 0.02
0.66MetTyr: 0.66 ± 0.036
0.0MetXaa: 0.0 ± 0.0
Asn
3.117AsnAla: 3.117 ± 0.093
0.895AsnCys: 0.895 ± 0.049
3.441AsnAsp: 3.441 ± 0.097
5.021AsnGlu: 5.021 ± 0.131
2.095AsnPhe: 2.095 ± 0.072
3.832AsnGly: 3.832 ± 0.098
1.011AsnHis: 1.011 ± 0.049
9.267AsnIle: 9.267 ± 0.18
7.459AsnLys: 7.459 ± 0.172
5.754AsnLeu: 5.754 ± 0.117
1.662AsnMet: 1.662 ± 0.056
7.02AsnAsn: 7.02 ± 0.228
3.09AsnPro: 3.09 ± 0.087
1.157AsnGln: 1.157 ± 0.053
1.824AsnArg: 1.824 ± 0.066
3.702AsnSer: 3.702 ± 0.101
3.557AsnThr: 3.557 ± 0.112
2.999AsnVal: 2.999 ± 0.09
0.458AsnTrp: 0.458 ± 0.034
3.224AsnTyr: 3.224 ± 0.104
0.0AsnXaa: 0.0 ± 0.0
Pro
1.735ProAla: 1.735 ± 0.064
0.449ProCys: 0.449 ± 0.034
1.806ProAsp: 1.806 ± 0.07
2.81ProGlu: 2.81 ± 0.09
1.457ProPhe: 1.457 ± 0.055
1.521ProGly: 1.521 ± 0.063
0.715ProHis: 0.715 ± 0.039
3.746ProIle: 3.746 ± 0.091
3.231ProLys: 3.231 ± 0.09
3.006ProLeu: 3.006 ± 0.088
0.913ProMet: 0.913 ± 0.046
2.225ProAsn: 2.225 ± 0.087
1.027ProPro: 1.027 ± 0.045
0.765ProGln: 0.765 ± 0.044
1.004ProArg: 1.004 ± 0.047
2.083ProSer: 2.083 ± 0.065
2.052ProThr: 2.052 ± 0.067
2.43ProVal: 2.43 ± 0.063
0.207ProTrp: 0.207 ± 0.024
1.491ProTyr: 1.491 ± 0.056
0.0ProXaa: 0.0 ± 0.0
Gln
0.811GlnAla: 0.811 ± 0.047
0.298GlnCys: 0.298 ± 0.026
0.854GlnAsp: 0.854 ± 0.043
1.052GlnGlu: 1.052 ± 0.047
0.697GlnPhe: 0.697 ± 0.038
0.988GlnGly: 0.988 ± 0.049
0.312GlnHis: 0.312 ± 0.029
1.778GlnIle: 1.778 ± 0.065
1.892GlnLys: 1.892 ± 0.057
1.628GlnLeu: 1.628 ± 0.06
0.617GlnMet: 0.617 ± 0.035
1.35GlnAsn: 1.35 ± 0.062
0.501GlnPro: 0.501 ± 0.03
0.51GlnGln: 0.51 ± 0.041
0.74GlnArg: 0.74 ± 0.049
0.929GlnSer: 0.929 ± 0.05
0.838GlnThr: 0.838 ± 0.045
0.89GlnVal: 0.89 ± 0.044
0.15GlnTrp: 0.15 ± 0.019
0.936GlnTyr: 0.936 ± 0.046
0.0GlnXaa: 0.0 ± 0.0
Arg
1.817ArgAla: 1.817 ± 0.075
0.496ArgCys: 0.496 ± 0.036
1.785ArgAsp: 1.785 ± 0.066
2.263ArgGlu: 2.263 ± 0.076
1.139ArgPhe: 1.139 ± 0.053
2.072ArgGly: 2.072 ± 0.075
0.558ArgHis: 0.558 ± 0.037
2.896ArgIle: 2.896 ± 0.085
2.794ArgLys: 2.794 ± 0.09
2.393ArgLeu: 2.393 ± 0.081
0.874ArgMet: 0.874 ± 0.055
1.671ArgAsn: 1.671 ± 0.062
1.084ArgPro: 1.084 ± 0.053
0.608ArgGln: 0.608 ± 0.039
1.202ArgArg: 1.202 ± 0.069
1.25ArgSer: 1.25 ± 0.059
1.519ArgThr: 1.519 ± 0.065
1.778ArgVal: 1.778 ± 0.061
0.225ArgTrp: 0.225 ± 0.024
1.223ArgTyr: 1.223 ± 0.051
0.0ArgXaa: 0.0 ± 0.0
Ser
3.083SerAla: 3.083 ± 0.098
0.685SerCys: 0.685 ± 0.036
2.748SerAsp: 2.748 ± 0.075
3.641SerGlu: 3.641 ± 0.094
2.277SerPhe: 2.277 ± 0.073
3.882SerGly: 3.882 ± 0.103
0.947SerHis: 0.947 ± 0.041
5.544SerIle: 5.544 ± 0.138
5.506SerLys: 5.506 ± 0.125
4.766SerLeu: 4.766 ± 0.114
1.359SerMet: 1.359 ± 0.059
3.452SerAsn: 3.452 ± 0.121
1.863SerPro: 1.863 ± 0.074
1.104SerGln: 1.104 ± 0.049
1.585SerArg: 1.585 ± 0.055
3.545SerSer: 3.545 ± 0.104
2.605SerThr: 2.605 ± 0.086
3.229SerVal: 3.229 ± 0.089
0.323SerTrp: 0.323 ± 0.03
2.131SerTyr: 2.131 ± 0.08
0.0SerXaa: 0.0 ± 0.0
Thr
2.808ThrAla: 2.808 ± 0.091
0.551ThrCys: 0.551 ± 0.037
2.43ThrAsp: 2.43 ± 0.069
3.069ThrGlu: 3.069 ± 0.089
1.475ThrPhe: 1.475 ± 0.067
3.566ThrGly: 3.566 ± 0.116
0.947ThrHis: 0.947 ± 0.048
5.8ThrIle: 5.8 ± 0.154
3.903ThrLys: 3.903 ± 0.099
3.755ThrLeu: 3.755 ± 0.098
1.164ThrMet: 1.164 ± 0.05
2.944ThrAsn: 2.944 ± 0.096
2.286ThrPro: 2.286 ± 0.079
0.847ThrGln: 0.847 ± 0.047
1.193ThrArg: 1.193 ± 0.06
2.418ThrSer: 2.418 ± 0.078
3.384ThrThr: 3.384 ± 0.137
3.595ThrVal: 3.595 ± 0.097
0.253ThrTrp: 0.253 ± 0.027
1.929ThrTyr: 1.929 ± 0.067
0.0ThrXaa: 0.0 ± 0.0
Val
3.967ValAla: 3.967 ± 0.114
1.047ValCys: 1.047 ± 0.061
3.773ValAsp: 3.773 ± 0.101
4.923ValGlu: 4.923 ± 0.114
2.379ValPhe: 2.379 ± 0.077
4.95ValGly: 4.95 ± 0.104
0.877ValHis: 0.877 ± 0.042
5.652ValIle: 5.652 ± 0.1
5.465ValLys: 5.465 ± 0.125
5.189ValLeu: 5.189 ± 0.114
1.546ValMet: 1.546 ± 0.062
3.443ValAsn: 3.443 ± 0.085
2.175ValPro: 2.175 ± 0.078
1.102ValGln: 1.102 ± 0.053
1.806ValArg: 1.806 ± 0.069
3.896ValSer: 3.896 ± 0.096
1.036ValThr: 1.036 ± 0.051
5.137ValVal: 5.137 ± 0.134
0.36ValTrp: 0.36 ± 0.028
2.131ValTyr: 2.131 ± 0.073
0.0ValXaa: 0.0 ± 0.0
Trp
0.362TrpAla: 0.362 ± 0.036
0.089TrpCys: 0.089 ± 0.015
0.451TrpAsp: 0.451 ± 0.028
0.449TrpGlu: 0.449 ± 0.033
0.228TrpPhe: 0.228 ± 0.024
0.435TrpGly: 0.435 ± 0.031
0.118TrpHis: 0.118 ± 0.017
0.587TrpIle: 0.587 ± 0.04
0.606TrpLys: 0.606 ± 0.047
0.398TrpLeu: 0.398 ± 0.03
0.166TrpMet: 0.166 ± 0.02
0.43TrpAsn: 0.43 ± 0.033
0.077TrpPro: 0.077 ± 0.012
0.123TrpGln: 0.123 ± 0.017
0.232TrpArg: 0.232 ± 0.02
0.351TrpSer: 0.351 ± 0.027
0.317TrpThr: 0.317 ± 0.028
0.403TrpVal: 0.403 ± 0.031
0.077TrpTrp: 0.077 ± 0.012
0.255TrpTyr: 0.255 ± 0.025
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.277TyrAla: 2.277 ± 0.071
0.633TyrCys: 0.633 ± 0.042
2.719TyrAsp: 2.719 ± 0.085
2.71TyrGlu: 2.71 ± 0.089
1.662TyrPhe: 1.662 ± 0.062
3.24TyrGly: 3.24 ± 0.076
0.567TyrHis: 0.567 ± 0.036
4.071TyrIle: 4.071 ± 0.104
3.313TyrLys: 3.313 ± 0.091
3.875TyrLeu: 3.875 ± 0.081
0.977TyrMet: 0.977 ± 0.049
3.468TyrAsn: 3.468 ± 0.101
1.589TyrPro: 1.589 ± 0.07
0.642TyrGln: 0.642 ± 0.042
1.12TyrArg: 1.12 ± 0.049
2.468TyrSer: 2.468 ± 0.069
1.858TyrThr: 1.858 ± 0.07
1.999TyrVal: 1.999 ± 0.061
0.287TyrTrp: 0.287 ± 0.023
1.979TyrTyr: 1.979 ± 0.076
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 1490 proteins (439175 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski