Beauveria bassiana D1-5
Average proteome isoelectric point is 6.53
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 11860 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0A2W2N7|A0A0A2W2N7_BEABA Serine/threonine-protein kinase nrc-2 OS=Beauveria bassiana D1-5 OX=1245745 GN=BBAD15_g1580 PE=4 SV=1
MM1 pKa = 6.93 KK2 pKa = 10.16 TFVALSALAAIATAQNVSSLAPCGQTCANNMLAASKK38 pKa = 10.37 AQEE41 pKa = 4.42 LDD43 pKa = 3.41 CSANDD48 pKa = 4.98 LKK50 pKa = 11.11 CLCDD54 pKa = 3.45 NRR56 pKa = 11.84 GFSLGLRR63 pKa = 11.84 DD64 pKa = 5.09 CSAATCSSDD73 pKa = 4.55 DD74 pKa = 3.42 AAQVVDD80 pKa = 5.77 FGVDD84 pKa = 3.46 LCRR87 pKa = 11.84 SASATGGGGGNGGNGASSTGGDD109 pKa = 3.55 GGSGGNGASSTGGDD123 pKa = 3.51 GGNGASSTDD132 pKa = 3.38 STSGSEE138 pKa = 4.22 ATSTSGPGNGVGGVGGAGGSGSSTDD163 pKa = 3.29 SGSGSEE169 pKa = 4.34 STGSDD174 pKa = 2.93 GGNGATSTGSNEE186 pKa = 3.92 ATSTGTGAGSEE197 pKa = 4.41 STSGSGSEE205 pKa = 4.29 TTGSEE210 pKa = 4.14 SGNGAGASSTQGSNGSEE227 pKa = 3.99 TTQAPTSSEE236 pKa = 3.89 GSSGNGGSSSTEE248 pKa = 3.69 GGAGASSTTGSQNYY262 pKa = 9.25 APQATAQVGLVAAAAGIAALMLL284 pKa = 4.5
Molecular weight: 25.6 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.772
IPC2_protein 3.656
IPC_protein 3.63
Toseland 3.427
ProMoST 3.808
Dawson 3.63
Bjellqvist 3.783
Wikipedia 3.567
Rodwell 3.465
Grimsley 3.338
Solomon 3.605
Lehninger 3.567
Nozaki 3.757
DTASelect 3.961
Thurlkill 3.49
EMBOSS 3.579
Sillero 3.757
Patrickios 0.54
IPC_peptide 3.605
IPC2_peptide 3.732
IPC2.peptide.svr19 3.695
Protein with the highest isoelectric point:
>tr|A0A0A2W488|A0A0A2W488_BEABA Putative serine/threonine-protein kinase OS=Beauveria bassiana D1-5 OX=1245745 GN=BBAD15_g6910 PE=4 SV=1
MM1 pKa = 7.64 RR2 pKa = 11.84 RR3 pKa = 11.84 QRR5 pKa = 11.84 RR6 pKa = 11.84 LLLPRR11 pKa = 11.84 LEE13 pKa = 4.5 VGALDD18 pKa = 3.93 PLAVLTAPPNPRR30 pKa = 11.84 DD31 pKa = 3.34 RR32 pKa = 11.84 RR33 pKa = 11.84 RR34 pKa = 11.84 ARR36 pKa = 11.84 LRR38 pKa = 11.84 RR39 pKa = 11.84 RR40 pKa = 11.84 PPARR44 pKa = 11.84 ARR46 pKa = 11.84 PPPAPRR52 pKa = 11.84 LRR54 pKa = 11.84 LLRR57 pKa = 11.84 GARR60 pKa = 11.84 GCAAMGRR67 pKa = 11.84 APRR70 pKa = 11.84 PEE72 pKa = 3.92 PAAPRR77 pKa = 11.84 ARR79 pKa = 11.84 LGRR82 pKa = 11.84 GARR85 pKa = 11.84 RR86 pKa = 11.84 GRR88 pKa = 11.84 ARR90 pKa = 11.84 RR91 pKa = 11.84 RR92 pKa = 11.84 AALASRR98 pKa = 11.84 PRR100 pKa = 11.84 RR101 pKa = 11.84 RR102 pKa = 11.84 RR103 pKa = 11.84 LRR105 pKa = 11.84 GPRR108 pKa = 11.84 TLGRR112 pKa = 11.84 LLPAALHH119 pKa = 5.89 VSLL122 pKa = 5.41
Molecular weight: 13.76 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.438
IPC2_protein 10.95
IPC_protein 12.574
Toseland 12.72
ProMoST 13.232
Dawson 12.72
Bjellqvist 12.72
Wikipedia 13.203
Rodwell 12.223
Grimsley 12.764
Solomon 13.232
Lehninger 13.13
Nozaki 12.72
DTASelect 12.72
Thurlkill 12.72
EMBOSS 13.232
Sillero 12.72
Patrickios 11.945
IPC_peptide 13.232
IPC2_peptide 12.223
IPC2.peptide.svr19 9.162
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
11840
20
11860
5904528
39
6489
497.9
54.84
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.761 ± 0.02
1.208 ± 0.009
5.785 ± 0.016
5.841 ± 0.022
3.627 ± 0.015
7.076 ± 0.024
2.424 ± 0.01
4.751 ± 0.016
4.58 ± 0.018
9.18 ± 0.024
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.27 ± 0.01
3.536 ± 0.013
5.683 ± 0.022
4.192 ± 0.018
6.221 ± 0.021
7.684 ± 0.024
5.787 ± 0.019
6.33 ± 0.016
1.429 ± 0.008
2.637 ± 0.012
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here