Georgenia soli
Average proteome isoelectric point is 6.11
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3768 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2A9ELZ2|A0A2A9ELZ2_9MICO Uracil-xanthine permease OS=Georgenia soli OX=638953 GN=ATJ97_1783 PE=3 SV=1
MM1 pKa = 7.33 HH2 pKa = 7.82 RR3 pKa = 11.84 RR4 pKa = 11.84 RR5 pKa = 11.84 LLASTAAAAAAVLALSACAPSDD27 pKa = 3.82 DD28 pKa = 4.96 APDD31 pKa = 4.47 PGADD35 pKa = 3.48 GGATSAAGSDD45 pKa = 3.51 EE46 pKa = 4.11 CAEE49 pKa = 4.08 PVALDD54 pKa = 3.21 VWSWRR59 pKa = 11.84 TEE61 pKa = 4.01 DD62 pKa = 3.37 KK63 pKa = 10.33 DD64 pKa = 3.65 TYY66 pKa = 9.8 EE67 pKa = 4.17 TIFDD71 pKa = 3.89 VFEE74 pKa = 4.18 EE75 pKa = 4.55 ANPCITVNFQAYY87 pKa = 9.78 KK88 pKa = 8.16 NTEE91 pKa = 3.98 YY92 pKa = 11.18 NQILQTGLTGSDD104 pKa = 3.85 GPDD107 pKa = 3.0 VAQVRR112 pKa = 11.84 SYY114 pKa = 11.78 GLLQPLVEE122 pKa = 4.97 GGNLAPLDD130 pKa = 4.07 DD131 pKa = 4.61 VVPALADD138 pKa = 3.64 FDD140 pKa = 4.41 EE141 pKa = 4.86 AALDD145 pKa = 3.82 GARR148 pKa = 11.84 GKK150 pKa = 10.59 EE151 pKa = 3.73 DD152 pKa = 2.93 GGIYY156 pKa = 10.09 GVPFAAQTIQVFYY169 pKa = 9.23 NTAIFEE175 pKa = 4.29 EE176 pKa = 4.92 HH177 pKa = 6.45 GLEE180 pKa = 4.96 EE181 pKa = 4.69 PEE183 pKa = 3.92 TWEE186 pKa = 5.36 DD187 pKa = 4.81 FIAANDD193 pKa = 3.92 TLKK196 pKa = 10.87 EE197 pKa = 4.09 AGVTPLAVGAKK208 pKa = 9.59 DD209 pKa = 2.93 AWFVPTVHH217 pKa = 7.4 DD218 pKa = 4.61 ALTAAQYY225 pKa = 11.1 GGAEE229 pKa = 4.22 FQQKK233 pKa = 10.02 LRR235 pKa = 11.84 DD236 pKa = 3.71 GEE238 pKa = 4.36 TDD240 pKa = 3.81 FNDD243 pKa = 3.47 PAYY246 pKa = 10.55 VKK248 pKa = 10.29 SIQSFADD255 pKa = 3.65 LEE257 pKa = 4.39 QYY259 pKa = 10.07 MPDD262 pKa = 3.58 DD263 pKa = 4.14 VVGVAYY269 pKa = 9.65 TDD271 pKa = 3.51 AQVLFTSGAAAMFAGGSYY289 pKa = 10.39 EE290 pKa = 4.06 LAFFQSTNPEE300 pKa = 3.94 LEE302 pKa = 4.13 LGVFQVPPPEE312 pKa = 5.08 GSALDD317 pKa = 4.07 HH318 pKa = 6.52 PVSPGYY324 pKa = 11.09 ADD326 pKa = 4.44 GNWGLSAASDD336 pKa = 3.71 NPEE339 pKa = 3.82 EE340 pKa = 5.83 AEE342 pKa = 4.48 TLLNWLASEE351 pKa = 4.69 EE352 pKa = 4.04 FGQMVANDD360 pKa = 4.74 LKK362 pKa = 10.97 QFSPVPGVTFDD373 pKa = 3.65 EE374 pKa = 4.79 PLMQEE379 pKa = 3.64 MWDD382 pKa = 4.21 LYY384 pKa = 10.58 QEE386 pKa = 4.29 NPAPYY391 pKa = 10.13 LLLVDD396 pKa = 3.99 YY397 pKa = 10.64 RR398 pKa = 11.84 YY399 pKa = 10.78 GDD401 pKa = 3.57 PVGTDD406 pKa = 3.06 LMGEE410 pKa = 4.77 GGQQLFLGEE419 pKa = 4.14 LDD421 pKa = 3.7 AAGVASKK428 pKa = 10.53 IQDD431 pKa = 3.74 GLSAWWTPGQQ441 pKa = 3.68
Molecular weight: 47.19 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.703
IPC2_protein 3.681
IPC_protein 3.694
Toseland 3.478
ProMoST 3.846
Dawson 3.681
Bjellqvist 3.834
Wikipedia 3.592
Rodwell 3.516
Grimsley 3.389
Solomon 3.668
Lehninger 3.63
Nozaki 3.783
DTASelect 4.012
Thurlkill 3.528
EMBOSS 3.605
Sillero 3.808
Patrickios 1.303
IPC_peptide 3.668
IPC2_peptide 3.795
IPC2.peptide.svr19 3.735
Protein with the highest isoelectric point:
>tr|A0A2A9EQ30|A0A2A9EQ30_9MICO Uncharacterized protein (DUF1697 family) OS=Georgenia soli OX=638953 GN=ATJ97_3620 PE=4 SV=1
MM1 pKa = 7.4 GSVIKK6 pKa = 10.38 KK7 pKa = 8.42 RR8 pKa = 11.84 RR9 pKa = 11.84 KK10 pKa = 9.06 RR11 pKa = 11.84 MSKK14 pKa = 9.76 KK15 pKa = 9.54 KK16 pKa = 9.79 HH17 pKa = 5.67 RR18 pKa = 11.84 KK19 pKa = 8.31 LLRR22 pKa = 11.84 KK23 pKa = 7.78 TRR25 pKa = 11.84 HH26 pKa = 3.65 QRR28 pKa = 11.84 RR29 pKa = 11.84 NKK31 pKa = 9.78 KK32 pKa = 9.85
Molecular weight: 4.1 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.493
IPC2_protein 11.14
IPC_protein 12.691
Toseland 12.866
ProMoST 13.349
Dawson 12.866
Bjellqvist 12.852
Wikipedia 13.334
Rodwell 12.735
Grimsley 12.91
Solomon 13.349
Lehninger 13.261
Nozaki 12.866
DTASelect 12.852
Thurlkill 12.866
EMBOSS 13.364
Sillero 12.866
Patrickios 12.457
IPC_peptide 13.349
IPC2_peptide 12.34
IPC2.peptide.svr19 9.071
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3768
0
3768
1259096
29
2069
334.2
35.55
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
14.005 ± 0.058
0.541 ± 0.009
6.103 ± 0.037
5.941 ± 0.042
2.547 ± 0.023
9.516 ± 0.037
2.133 ± 0.02
3.079 ± 0.031
1.569 ± 0.026
10.265 ± 0.049
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.789 ± 0.016
1.664 ± 0.02
5.934 ± 0.034
2.665 ± 0.021
7.918 ± 0.047
4.952 ± 0.028
6.22 ± 0.031
9.829 ± 0.04
1.457 ± 0.017
1.874 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here