Georgenia soli 
Average proteome isoelectric point is 6.11 
Get precalculated fractions of proteins 
 
  
    Acidic  
     
   
 
  
    pI < 6.8  
     
   
 
  
    6.8-7.4  
     
   
 
  
    pI > 7.4  
     
   
 
  
    Basic  
     
   
    
 
  
    All  
     
   
 
 
Note: above files contain also dissociation constants (pKa) 
 
Virtual 2D-PAGE plot for 3768 proteins (isoelectric point calculated using IPC2_protein) 
 
Get csv file with sequences according to given criteria: 
* You can choose from 21 different methods for calculating isoelectric point 
 
 Summary statistics related to proteome-wise predictions 
 
Protein with the lowest isoelectric point: 
>tr|A0A2A9ELZ2|A0A2A9ELZ2_9MICO Uracil-xanthine permease OS=Georgenia soli OX=638953 GN=ATJ97_1783 PE=3 SV=1 
MM1 pKa = 7.33  HH2 pKa = 7.82  RR3 pKa = 11.84  RR4 pKa = 11.84  RR5 pKa = 11.84  LLASTAAAAAAVLALSACAPSDD27 pKa = 3.82  DD28 pKa = 4.96  APDD31 pKa = 4.47  PGADD35 pKa = 3.48  GGATSAAGSDD45 pKa = 3.51  EE46 pKa = 4.11  CAEE49 pKa = 4.08  PVALDD54 pKa = 3.21  VWSWRR59 pKa = 11.84  TEE61 pKa = 4.01  DD62 pKa = 3.37  KK63 pKa = 10.33  DD64 pKa = 3.65  TYY66 pKa = 9.8  EE67 pKa = 4.17  TIFDD71 pKa = 3.89  VFEE74 pKa = 4.18  EE75 pKa = 4.55  ANPCITVNFQAYY87 pKa = 9.78  KK88 pKa = 8.16  NTEE91 pKa = 3.98  YY92 pKa = 11.18  NQILQTGLTGSDD104 pKa = 3.85  GPDD107 pKa = 3.0  VAQVRR112 pKa = 11.84  SYY114 pKa = 11.78  GLLQPLVEE122 pKa = 4.97  GGNLAPLDD130 pKa = 4.07  DD131 pKa = 4.61  VVPALADD138 pKa = 3.64  FDD140 pKa = 4.41  EE141 pKa = 4.86  AALDD145 pKa = 3.82  GARR148 pKa = 11.84  GKK150 pKa = 10.59  EE151 pKa = 3.73  DD152 pKa = 2.93  GGIYY156 pKa = 10.09  GVPFAAQTIQVFYY169 pKa = 9.23  NTAIFEE175 pKa = 4.29  EE176 pKa = 4.92  HH177 pKa = 6.45  GLEE180 pKa = 4.96  EE181 pKa = 4.69  PEE183 pKa = 3.92  TWEE186 pKa = 5.36  DD187 pKa = 4.81  FIAANDD193 pKa = 3.92  TLKK196 pKa = 10.87  EE197 pKa = 4.09  AGVTPLAVGAKK208 pKa = 9.59  DD209 pKa = 2.93  AWFVPTVHH217 pKa = 7.4  DD218 pKa = 4.61  ALTAAQYY225 pKa = 11.1  GGAEE229 pKa = 4.22  FQQKK233 pKa = 10.02  LRR235 pKa = 11.84  DD236 pKa = 3.71  GEE238 pKa = 4.36  TDD240 pKa = 3.81  FNDD243 pKa = 3.47  PAYY246 pKa = 10.55  VKK248 pKa = 10.29  SIQSFADD255 pKa = 3.65  LEE257 pKa = 4.39  QYY259 pKa = 10.07  MPDD262 pKa = 3.58  DD263 pKa = 4.14  VVGVAYY269 pKa = 9.65  TDD271 pKa = 3.51  AQVLFTSGAAAMFAGGSYY289 pKa = 10.39  EE290 pKa = 4.06  LAFFQSTNPEE300 pKa = 3.94  LEE302 pKa = 4.13  LGVFQVPPPEE312 pKa = 5.08  GSALDD317 pKa = 4.07  HH318 pKa = 6.52  PVSPGYY324 pKa = 11.09  ADD326 pKa = 4.44  GNWGLSAASDD336 pKa = 3.71  NPEE339 pKa = 3.82  EE340 pKa = 5.83  AEE342 pKa = 4.48  TLLNWLASEE351 pKa = 4.69  EE352 pKa = 4.04  FGQMVANDD360 pKa = 4.74  LKK362 pKa = 10.97  QFSPVPGVTFDD373 pKa = 3.65  EE374 pKa = 4.79  PLMQEE379 pKa = 3.64  MWDD382 pKa = 4.21  LYY384 pKa = 10.58  QEE386 pKa = 4.29  NPAPYY391 pKa = 10.13  LLLVDD396 pKa = 3.99  YY397 pKa = 10.64  RR398 pKa = 11.84  YY399 pKa = 10.78  GDD401 pKa = 3.57  PVGTDD406 pKa = 3.06  LMGEE410 pKa = 4.77  GGQQLFLGEE419 pKa = 4.14  LDD421 pKa = 3.7  AAGVASKK428 pKa = 10.53  IQDD431 pKa = 3.74  GLSAWWTPGQQ441 pKa = 3.68   
 Molecular weight: 47.19 kDa
Isoelectric point according different methods: 
IPC2.protein.svr19  3.703 
IPC2_protein 3.681 
IPC_protein 3.694 
Toseland    3.478 
ProMoST     3.846 
Dawson      3.681 
Bjellqvist  3.834 
Wikipedia   3.592 
Rodwell     3.516 
Grimsley    3.389 
Solomon     3.668 
Lehninger   3.63 
Nozaki      3.783 
DTASelect   4.012 
Thurlkill   3.528 
EMBOSS      3.605 
Sillero     3.808 
Patrickios  1.303 
IPC_peptide 3.668 
IPC2_peptide  3.795 
IPC2.peptide.svr19  3.735 
 Protein with the highest isoelectric point: 
>tr|A0A2A9EQ30|A0A2A9EQ30_9MICO Uncharacterized protein (DUF1697 family) OS=Georgenia soli OX=638953 GN=ATJ97_3620 PE=4 SV=1 
MM1 pKa = 7.4  GSVIKK6 pKa = 10.38  KK7 pKa = 8.42  RR8 pKa = 11.84  RR9 pKa = 11.84  KK10 pKa = 9.06  RR11 pKa = 11.84  MSKK14 pKa = 9.76  KK15 pKa = 9.54  KK16 pKa = 9.79  HH17 pKa = 5.67  RR18 pKa = 11.84  KK19 pKa = 8.31  LLRR22 pKa = 11.84  KK23 pKa = 7.78  TRR25 pKa = 11.84  HH26 pKa = 3.65  QRR28 pKa = 11.84  RR29 pKa = 11.84  NKK31 pKa = 9.78  KK32 pKa = 9.85   
 Molecular weight: 4.1 kDa
Isoelectric point according different methods: 
IPC2.protein.svr19  9.493 
IPC2_protein 11.14 
IPC_protein 12.691 
Toseland    12.866 
ProMoST     13.349 
Dawson      12.866 
Bjellqvist  12.852 
Wikipedia   13.334 
Rodwell     12.735 
Grimsley    12.91 
Solomon     13.349 
Lehninger   13.261 
Nozaki      12.866 
DTASelect   12.852 
Thurlkill   12.866 
EMBOSS      13.364 
Sillero     12.866 
Patrickios  12.457 
IPC_peptide 13.349 
IPC2_peptide  12.34 
IPC2.peptide.svr19  9.071 
  
 
Peptides (in silico  digests for buttom-up proteomics) 
Below you can find 
in silico  digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
 
  
    Try ESI  
     
   
 
  
    ChTry ESI  
     
   
 
  
    ArgC ESI  
     
   
 
  
    LysN ESI  
     
   
 
  
    TryLysC ESI  
     
   
 
  
    Try MALDI  
     
   
 
  
    ChTry MALDI  
     
   
 
  
    ArgC MALDI  
     
   
 
  
    LysN MALDI  
     
   
 
  
    TryLysC MALDI  
     
   
 
  
    Try LTQ  
     
   
 
  
    ChTry LTQ  
     
   
 
  
    ArgC LTQ  
     
   
 
  
    LysN LTQ  
     
   
 
  
    TryLysC LTQ  
     
   
 
  
    Try MSlow  
     
   
 
  
    ChTry MSlow  
     
   
 
  
    ArgC MSlow  
     
   
 
  
    LysN MSlow  
     
   
 
  
    TryLysC MSlow  
     
   
 
  
    Try MShigh  
     
   
 
  
    ChTry MShigh  
     
   
 
  
    ArgC MShigh  
     
   
 
  
    LysN MShigh  
     
   
 
  
    TryLysC MShigh  
     
   
   
  
                       
General Statistics 
    
      
        Number of major isoforms
 
        Number of additional isoforms
 
        Number of all proteins
         
        Number of amino acids
 
        Min. Seq. Length
 
        Max. Seq. Length
 
        Avg. Seq. Length
 
        Avg. Mol. Weight
 
       
     
    
      
        3768 
 
        
        0
 
        
        3768 
         
        1259096
 
        29
 
        2069
 
        334.2
 
        35.55
 
                
     
  
 
    Amino acid frequency 
  
  
    
      
        Ala
 
        Cys
 
        Asp
 
        Glu
 
        Phe
 
        Gly
 
        His
 
        Ile
 
        Lys
 
        Leu
 
       
     
    
                 
        14.005 ± 0.058
0.541 ± 0.009
 
        6.103 ± 0.037
5.941 ± 0.042
 
        2.547 ± 0.023
9.516 ± 0.037
 
        2.133 ± 0.02
3.079 ± 0.031
       
        1.569 ± 0.026
10.265 ± 0.049
         
                
     
  
  
  
      
          
        Met
         
        Asn
 
        Gln
 
        Pro
         
        Arg
 
        Ser
 
        Thr
 
        Val
 
        Trp
 
        Tyr
  
       
     
    
                 
        1.789 ± 0.016
1.664 ± 0.02
 
        5.934 ± 0.034
2.665 ± 0.021
 
        7.918 ± 0.047
4.952 ± 0.028
 
        6.22 ± 0.031
9.829 ± 0.04
       
        1.457 ± 0.017
1.874 ± 0.018
         
                
     
  
  
  
Note:  For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level 
Most of the basic statistics you can see at this page can be downloaded from this CSV file  
For dipeptide frequency statistics click here