Stagnimonas aquatica

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Nevskiales; Sinobacteraceae; Stagnimonas

Average proteome isoelectric point is 6.83

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3293 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3N0VBQ8|A0A3N0VBQ8_9GAMM Efflux RND transporter periplasmic adaptor subunit OS=Stagnimonas aquatica OX=2689987 GN=ED208_11285 PE=3 SV=1
MM1 pKa = 7.52ALAVSLFLLILVTLIGLAAIRR22 pKa = 11.84GTTIQQRR29 pKa = 11.84MTANFYY35 pKa = 10.86DD36 pKa = 4.29RR37 pKa = 11.84EE38 pKa = 4.14IAFQSAEE45 pKa = 3.73AGLRR49 pKa = 11.84AGEE52 pKa = 4.94AYY54 pKa = 10.62LSDD57 pKa = 4.36PGSPLVDD64 pKa = 3.06GVDD67 pKa = 4.34FRR69 pKa = 11.84NCQDD73 pKa = 4.24GDD75 pKa = 3.8TCLANPFADD84 pKa = 3.7SGLGTGFIKK93 pKa = 9.97TVDD96 pKa = 3.33TDD98 pKa = 4.32DD99 pKa = 4.83YY100 pKa = 11.85DD101 pKa = 4.92VSDD104 pKa = 4.3TNAPGQPQFVIEE116 pKa = 5.13LICLNNACSAGAGSTGNCQSANCQSYY142 pKa = 8.27GTEE145 pKa = 3.64NTAAADD151 pKa = 3.56FAYY154 pKa = 10.74YY155 pKa = 10.45RR156 pKa = 11.84ITARR160 pKa = 11.84SGDD163 pKa = 3.91PGSEE167 pKa = 3.68AGTNRR172 pKa = 11.84AVVILQAVFQVPLSS186 pKa = 3.67

Molecular weight: 19.45 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3N0VLW3|A0A3N0VLW3_9GAMM Diaminopimelate decarboxylase OS=Stagnimonas aquatica OX=2689987 GN=lysA PE=3 SV=1
MM1 pKa = 7.74SKK3 pKa = 8.96RR4 pKa = 11.84TYY6 pKa = 9.73QPHH9 pKa = 5.47AVRR12 pKa = 11.84RR13 pKa = 11.84KK14 pKa = 7.53RR15 pKa = 11.84THH17 pKa = 5.8GFRR20 pKa = 11.84ARR22 pKa = 11.84MATAGGRR29 pKa = 11.84LVLSRR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84AKK38 pKa = 9.98GRR40 pKa = 11.84KK41 pKa = 8.89RR42 pKa = 11.84LIPP45 pKa = 4.02

Molecular weight: 5.29 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3293

0

3293

1144562

32

2763

347.6

37.69

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.978 ± 0.069

0.891 ± 0.013

5.078 ± 0.031

5.679 ± 0.034

3.304 ± 0.026

8.609 ± 0.042

2.007 ± 0.02

3.897 ± 0.029

3.097 ± 0.039

11.898 ± 0.068

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.863 ± 0.019

2.542 ± 0.033

5.638 ± 0.042

3.958 ± 0.025

7.333 ± 0.043

5.693 ± 0.039

4.599 ± 0.04

6.915 ± 0.034

1.455 ± 0.021

2.564 ± 0.025
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski