Shewanella maritima
Average proteome isoelectric point is 6.07
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3709 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A411PJ71|A0A411PJ71_9GAMM Response regulator OS=Shewanella maritima OX=2520507 GN=EXU30_13670 PE=4 SV=1
MM1 pKa = 7.34 KK2 pKa = 10.17 RR3 pKa = 11.84 IYY5 pKa = 10.05 PIALAASLIALAGCSDD21 pKa = 4.59 DD22 pKa = 5.28 DD23 pKa = 4.45 NDD25 pKa = 5.07 VITIEE30 pKa = 3.9 QDD32 pKa = 3.14 EE33 pKa = 4.91 PQTMAYY39 pKa = 9.88 VRR41 pKa = 11.84 VIHH44 pKa = 6.72 AGSDD48 pKa = 3.47 APKK51 pKa = 10.91 VNVNVNGSQLLADD64 pKa = 3.83 VDD66 pKa = 4.27 YY67 pKa = 11.58 AMSSGLIQVDD77 pKa = 3.51 TATYY81 pKa = 10.26 DD82 pKa = 3.21 IDD84 pKa = 3.7 VDD86 pKa = 4.95 AILADD91 pKa = 3.72 GTTATVLEE99 pKa = 4.73 TNLSTEE105 pKa = 3.75 ADD107 pKa = 3.44 MEE109 pKa = 4.39 YY110 pKa = 9.68 TAVAVGSVAEE120 pKa = 4.12 EE121 pKa = 3.82 TLALKK126 pKa = 10.75 LIANPMAAIASGYY139 pKa = 10.88 ARR141 pKa = 11.84 VQVLHH146 pKa = 6.61 AAADD150 pKa = 3.59 VGLVDD155 pKa = 5.05 IYY157 pKa = 10.39 VTEE160 pKa = 4.54 PGADD164 pKa = 3.35 IDD166 pKa = 4.16 MMSPTLSANYY176 pKa = 8.94 MDD178 pKa = 5.07 NSEE181 pKa = 4.15 QLEE184 pKa = 4.59 LATGDD189 pKa = 3.47 YY190 pKa = 10.16 QIRR193 pKa = 11.84 ITASGMKK200 pKa = 10.11 DD201 pKa = 3.27 VVYY204 pKa = 10.79 DD205 pKa = 3.65 SGTITLGDD213 pKa = 3.46 MTDD216 pKa = 3.8 YY217 pKa = 10.89 LVSAITNTWSGDD229 pKa = 3.45 APVALHH235 pKa = 6.27 IALVEE240 pKa = 4.17 GQVIVNDD247 pKa = 4.13 ANSGADD253 pKa = 3.33 LRR255 pKa = 11.84 VIHH258 pKa = 6.69 AVADD262 pKa = 3.87 APEE265 pKa = 4.19 VDD267 pKa = 4.23 VFLDD271 pKa = 3.99 DD272 pKa = 3.7 ATSPAVDD279 pKa = 3.31 MLAFKK284 pKa = 11.04 AFTPYY289 pKa = 10.96 VNIPEE294 pKa = 4.38 GMHH297 pKa = 5.52 TVTVAADD304 pKa = 3.5 ADD306 pKa = 3.88 NSVVVIDD313 pKa = 4.44 KK314 pKa = 11.23 APIEE318 pKa = 4.03 PMIGMSYY325 pKa = 10.56 SAFAVGSLSDD335 pKa = 5.19 DD336 pKa = 3.63 MIEE339 pKa = 4.14 PLVLMEE345 pKa = 4.04 DD346 pKa = 3.56 TRR348 pKa = 11.84 RR349 pKa = 11.84 VATEE353 pKa = 3.39 AKK355 pKa = 10.59 LNVLHH360 pKa = 6.54 SAYY363 pKa = 10.32 SAPEE367 pKa = 3.49 VDD369 pKa = 5.26 IYY371 pKa = 11.01 LTDD374 pKa = 3.55 SADD377 pKa = 3.26 ISEE380 pKa = 4.73 ATPALTSVPFKK391 pKa = 9.91 MASGSLSIAPGNYY404 pKa = 8.03 TISVAVAGTKK414 pKa = 8.88 TVAIGPLDD422 pKa = 3.59 VTLEE426 pKa = 3.94 AGGIYY431 pKa = 10.22 GVAAVDD437 pKa = 3.66 NMGGGAPFGVILLDD451 pKa = 4.36 DD452 pKa = 5.23 FNAEE456 pKa = 3.99 MM457 pKa = 5.18
Molecular weight: 47.62 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.681
IPC2_protein 3.681
IPC_protein 3.719
Toseland 3.478
ProMoST 3.884
Dawson 3.719
Bjellqvist 3.872
Wikipedia 3.668
Rodwell 3.541
Grimsley 3.389
Solomon 3.706
Lehninger 3.668
Nozaki 3.821
DTASelect 4.101
Thurlkill 3.541
EMBOSS 3.668
Sillero 3.846
Patrickios 1.329
IPC_peptide 3.706
IPC2_peptide 3.821
IPC2.peptide.svr19 3.749
Protein with the highest isoelectric point:
>tr|A0A411PI14|A0A411PI14_9GAMM Der GTPase-activating protein YihI OS=Shewanella maritima OX=2520507 GN=yihI PE=3 SV=1
MM1 pKa = 7.69 SKK3 pKa = 9.01 RR4 pKa = 11.84 TFQPSNLKK12 pKa = 10.14 RR13 pKa = 11.84 KK14 pKa = 9.13 RR15 pKa = 11.84 SHH17 pKa = 6.17 GFRR20 pKa = 11.84 ARR22 pKa = 11.84 MATVGGRR29 pKa = 11.84 KK30 pKa = 9.01 VLARR34 pKa = 11.84 RR35 pKa = 11.84 RR36 pKa = 11.84 AKK38 pKa = 10.39 GRR40 pKa = 11.84 ARR42 pKa = 11.84 LSAA45 pKa = 3.91
Molecular weight: 5.15 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.523
IPC2_protein 11.242
IPC_protein 12.837
Toseland 13.013
ProMoST 13.495
Dawson 13.013
Bjellqvist 12.998
Wikipedia 13.481
Rodwell 12.676
Grimsley 13.042
Solomon 13.495
Lehninger 13.408
Nozaki 13.013
DTASelect 12.998
Thurlkill 13.013
EMBOSS 13.51
Sillero 13.013
Patrickios 12.398
IPC_peptide 13.51
IPC2_peptide 12.486
IPC2.peptide.svr19 9.177
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3709
0
3709
1257756
27
8132
339.1
37.52
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.186 ± 0.042
1.009 ± 0.016
5.797 ± 0.05
5.957 ± 0.043
3.994 ± 0.029
6.73 ± 0.038
2.228 ± 0.024
6.084 ± 0.031
5.256 ± 0.047
10.136 ± 0.056
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.583 ± 0.025
4.453 ± 0.048
3.784 ± 0.024
5.173 ± 0.044
4.124 ± 0.047
6.75 ± 0.04
5.47 ± 0.091
7.037 ± 0.037
1.171 ± 0.014
3.078 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here