Escherichia phage T5 (Enterobacteria phage T5)
Average proteome isoelectric point is 6.59
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 163 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|Q6QGI8|Q6QGI8_BPT5 Putative anaerobic ribonucleoside-triphosphate reductase OS=Escherichia phage T5 OX=10726 GN=nrdD PE=4 SV=1
MM1 pKa = 7.21 TLKK4 pKa = 10.48 PYY6 pKa = 10.08 IVEE9 pKa = 4.3 STCGGFHH16 pKa = 7.37 LGVDD20 pKa = 4.97 DD21 pKa = 3.69 IAEE24 pKa = 4.16 AVFAATGDD32 pKa = 3.67 YY33 pKa = 11.11 SHH35 pKa = 7.72 IEE37 pKa = 3.84 GAGYY41 pKa = 7.39 PTVIGDD47 pKa = 3.74 WVEE50 pKa = 4.19 TNLDD54 pKa = 3.42 VSPYY58 pKa = 10.8 NPYY61 pKa = 10.86 CLFDD65 pKa = 3.75 EE66 pKa = 5.45 DD67 pKa = 4.97 RR68 pKa = 11.84 DD69 pKa = 3.96 EE70 pKa = 4.54 VKK72 pKa = 11.0 CNLEE76 pKa = 3.75 FVYY79 pKa = 10.95 NRR81 pKa = 11.84 VLKK84 pKa = 10.38 VYY86 pKa = 9.99 QRR88 pKa = 11.84 SPDD91 pKa = 3.37 GYY93 pKa = 11.2 EE94 pKa = 5.91 DD95 pKa = 3.29 ITCSIKK101 pKa = 10.57 QYY103 pKa = 11.06 LKK105 pKa = 10.67 DD106 pKa = 3.72 VYY108 pKa = 11.26 SSFSDD113 pKa = 3.38 EE114 pKa = 4.49 DD115 pKa = 4.21 LEE117 pKa = 4.65 TVAVEE122 pKa = 4.54 FNNDD126 pKa = 2.7
Molecular weight: 14.26 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.866
IPC2_protein 3.948
IPC_protein 3.923
Toseland 3.719
ProMoST 4.024
Dawson 3.897
Bjellqvist 4.088
Wikipedia 3.821
Rodwell 3.745
Grimsley 3.63
Solomon 3.897
Lehninger 3.846
Nozaki 4.012
DTASelect 4.228
Thurlkill 3.757
EMBOSS 3.834
Sillero 4.037
Patrickios 0.337
IPC_peptide 3.884
IPC2_peptide 4.012
IPC2.peptide.svr19 3.953
Protein with the highest isoelectric point:
>tr|Q6QGG6|Q6QGG6_BPT5 Putative DNA replication primase OS=Escherichia phage T5 OX=10726 GN=pri PE=4 SV=1
MM1 pKa = 7.28 IASRR5 pKa = 11.84 DD6 pKa = 3.88 CFVDD10 pKa = 3.87 RR11 pKa = 11.84 VFSSIFICRR20 pKa = 11.84 AVFRR24 pKa = 11.84 NNRR27 pKa = 11.84 MFIPFVIADD36 pKa = 3.49 EE37 pKa = 4.89 TIFEE41 pKa = 4.58 RR42 pKa = 11.84 IKK44 pKa = 10.77 PKK46 pKa = 10.07 TFHH49 pKa = 7.38 DD50 pKa = 4.71 LNCTLHH56 pKa = 6.13 GQTAVFANIIHH67 pKa = 6.2 VSLLLPRR74 pKa = 11.84 PKK76 pKa = 10.36 PNLLRR81 pKa = 11.84 NSFGRR86 pKa = 11.84 FGFRR90 pKa = 11.84 IAHH93 pKa = 6.73 GSNIVLQSSIGGVNCTIYY111 pKa = 10.61 GVNLLNPTRR120 pKa = 11.84 SPAA123 pKa = 3.19
Molecular weight: 13.85 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.348
IPC2_protein 9.385
IPC_protein 10.101
Toseland 10.57
ProMoST 10.277
Dawson 10.657
Bjellqvist 10.409
Wikipedia 10.862
Rodwell 10.76
Grimsley 10.687
Solomon 10.804
Lehninger 10.789
Nozaki 10.643
DTASelect 10.365
Thurlkill 10.57
EMBOSS 10.979
Sillero 10.613
Patrickios 10.599
IPC_peptide 10.818
IPC2_peptide 9.897
IPC2.peptide.svr19 8.506
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
162
1
163
32793
27
1396
201.2
22.64
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.352 ± 0.373
1.189 ± 0.096
5.952 ± 0.154
6.703 ± 0.224
4.086 ± 0.157
6.459 ± 0.261
1.863 ± 0.13
6.617 ± 0.177
7.319 ± 0.235
8.499 ± 0.245
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.641 ± 0.132
5.492 ± 0.188
3.525 ± 0.132
3.723 ± 0.219
4.589 ± 0.132
6.617 ± 0.284
5.855 ± 0.189
6.382 ± 0.147
1.208 ± 0.09
3.928 ± 0.162
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here