Amino acid dipepetide frequency for Escherichia phage T5 (Enterobacteria phage T5)

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
7.044AlaAla: 7.044 ± 0.92
0.762AlaCys: 0.762 ± 0.15
3.659AlaAsp: 3.659 ± 0.338
5.062AlaGlu: 5.062 ± 0.42
2.989AlaPhe: 2.989 ± 0.248
4.879AlaGly: 4.879 ± 0.461
1.342AlaHis: 1.342 ± 0.224
4.727AlaIle: 4.727 ± 0.37
6.587AlaLys: 6.587 ± 0.583
5.886AlaLeu: 5.886 ± 0.639
2.196AlaMet: 2.196 ± 0.298
3.324AlaAsn: 3.324 ± 0.356
2.135AlaPro: 2.135 ± 0.269
3.293AlaGln: 3.293 ± 0.356
3.08AlaArg: 3.08 ± 0.305
4.818AlaSer: 4.818 ± 0.555
3.873AlaThr: 3.873 ± 0.49
4.483AlaVal: 4.483 ± 0.463
0.915AlaTrp: 0.915 ± 0.201
2.47AlaTyr: 2.47 ± 0.312
0.0AlaXaa: 0.0 ± 0.0
Cys
0.671CysAla: 0.671 ± 0.146
0.335CysCys: 0.335 ± 0.147
0.488CysAsp: 0.488 ± 0.099
0.854CysGlu: 0.854 ± 0.157
0.549CysPhe: 0.549 ± 0.142
0.976CysGly: 0.976 ± 0.189
0.366CysHis: 0.366 ± 0.11
0.671CysIle: 0.671 ± 0.14
0.793CysLys: 0.793 ± 0.165
0.854CysLeu: 0.854 ± 0.145
0.183CysMet: 0.183 ± 0.081
0.701CysAsn: 0.701 ± 0.145
0.579CysPro: 0.579 ± 0.165
0.396CysGln: 0.396 ± 0.114
0.549CysArg: 0.549 ± 0.131
1.067CysSer: 1.067 ± 0.154
0.64CysThr: 0.64 ± 0.137
0.518CysVal: 0.518 ± 0.134
0.152CysTrp: 0.152 ± 0.072
0.549CysTyr: 0.549 ± 0.139
0.0CysXaa: 0.0 ± 0.0
Asp
4.147AspAla: 4.147 ± 0.365
0.671AspCys: 0.671 ± 0.153
2.562AspAsp: 2.562 ± 0.302
4.086AspGlu: 4.086 ± 0.444
2.623AspPhe: 2.623 ± 0.296
4.147AspGly: 4.147 ± 0.299
1.433AspHis: 1.433 ± 0.19
4.178AspIle: 4.178 ± 0.405
4.3AspLys: 4.3 ± 0.351
5.611AspLeu: 5.611 ± 0.4
1.769AspMet: 1.769 ± 0.219
2.867AspAsn: 2.867 ± 0.315
2.623AspPro: 2.623 ± 0.234
1.555AspGln: 1.555 ± 0.203
2.592AspArg: 2.592 ± 0.241
3.934AspSer: 3.934 ± 0.33
3.69AspThr: 3.69 ± 0.345
3.507AspVal: 3.507 ± 0.304
0.732AspTrp: 0.732 ± 0.133
2.501AspTyr: 2.501 ± 0.309
0.0AspXaa: 0.0 ± 0.0
Glu
5.215GluAla: 5.215 ± 0.417
0.64GluCys: 0.64 ± 0.153
3.293GluAsp: 3.293 ± 0.324
4.727GluGlu: 4.727 ± 0.514
2.287GluPhe: 2.287 ± 0.257
4.147GluGly: 4.147 ± 0.391
1.525GluHis: 1.525 ± 0.218
4.696GluIle: 4.696 ± 0.382
4.696GluLys: 4.696 ± 0.402
7.319GluLeu: 7.319 ± 0.49
2.257GluMet: 2.257 ± 0.283
2.928GluAsn: 2.928 ± 0.301
1.372GluPro: 1.372 ± 0.212
2.989GluGln: 2.989 ± 0.416
3.111GluArg: 3.111 ± 0.341
3.476GluSer: 3.476 ± 0.291
3.873GluThr: 3.873 ± 0.332
4.239GluVal: 4.239 ± 0.374
1.006GluTrp: 1.006 ± 0.182
3.232GluTyr: 3.232 ± 0.35
0.0GluXaa: 0.0 ± 0.0
Phe
2.623PheAla: 2.623 ± 0.289
0.274PheCys: 0.274 ± 0.111
2.867PheAsp: 2.867 ± 0.327
2.47PheGlu: 2.47 ± 0.293
1.281PhePhe: 1.281 ± 0.187
2.989PheGly: 2.989 ± 0.365
1.067PheHis: 1.067 ± 0.183
2.958PheIle: 2.958 ± 0.342
2.958PheLys: 2.958 ± 0.322
3.293PheLeu: 3.293 ± 0.298
0.854PheMet: 0.854 ± 0.13
2.775PheAsn: 2.775 ± 0.299
1.464PhePro: 1.464 ± 0.242
0.884PheGln: 0.884 ± 0.148
1.83PheArg: 1.83 ± 0.263
3.172PheSer: 3.172 ± 0.34
2.348PheThr: 2.348 ± 0.237
2.714PheVal: 2.714 ± 0.307
0.518PheTrp: 0.518 ± 0.127
1.525PheTyr: 1.525 ± 0.236
0.0PheXaa: 0.0 ± 0.0
Gly
4.757GlyAla: 4.757 ± 0.61
1.006GlyCys: 1.006 ± 0.214
4.208GlyAsp: 4.208 ± 0.434
4.147GlyGlu: 4.147 ± 0.352
3.202GlyPhe: 3.202 ± 0.307
3.537GlyGly: 3.537 ± 0.507
1.159GlyHis: 1.159 ± 0.236
4.757GlyIle: 4.757 ± 0.403
5.062GlyLys: 5.062 ± 0.415
4.94GlyLeu: 4.94 ± 0.39
1.891GlyMet: 1.891 ± 0.309
3.202GlyAsn: 3.202 ± 0.31
1.22GlyPro: 1.22 ± 0.206
2.135GlyGln: 2.135 ± 0.284
2.897GlyArg: 2.897 ± 0.278
4.635GlySer: 4.635 ± 0.481
3.598GlyThr: 3.598 ± 0.386
4.391GlyVal: 4.391 ± 0.293
0.915GlyTrp: 0.915 ± 0.17
2.928GlyTyr: 2.928 ± 0.346
0.0GlyXaa: 0.0 ± 0.0
His
1.067HisAla: 1.067 ± 0.195
0.366HisCys: 0.366 ± 0.142
1.372HisAsp: 1.372 ± 0.229
1.037HisGlu: 1.037 ± 0.184
0.732HisPhe: 0.732 ± 0.169
1.281HisGly: 1.281 ± 0.192
0.549HisHis: 0.549 ± 0.179
1.372HisIle: 1.372 ± 0.197
1.372HisLys: 1.372 ± 0.233
1.891HisLeu: 1.891 ± 0.235
0.396HisMet: 0.396 ± 0.121
0.884HisAsn: 0.884 ± 0.201
0.884HisPro: 0.884 ± 0.17
0.549HisGln: 0.549 ± 0.147
1.281HisArg: 1.281 ± 0.209
1.067HisSer: 1.067 ± 0.184
0.884HisThr: 0.884 ± 0.172
0.884HisVal: 0.884 ± 0.151
0.091HisTrp: 0.091 ± 0.057
0.671HisTyr: 0.671 ± 0.128
0.0HisXaa: 0.0 ± 0.0
Ile
4.91IleAla: 4.91 ± 0.42
0.945IleCys: 0.945 ± 0.151
4.269IleAsp: 4.269 ± 0.417
4.452IleGlu: 4.452 ± 0.374
2.44IlePhe: 2.44 ± 0.307
4.086IleGly: 4.086 ± 0.346
1.098IleHis: 1.098 ± 0.169
4.147IleIle: 4.147 ± 0.292
4.483IleLys: 4.483 ± 0.38
5.428IleLeu: 5.428 ± 0.39
1.647IleMet: 1.647 ± 0.278
4.239IleAsn: 4.239 ± 0.392
2.836IlePro: 2.836 ± 0.336
2.165IleGln: 2.165 ± 0.241
2.836IleArg: 2.836 ± 0.256
4.33IleSer: 4.33 ± 0.371
4.605IleThr: 4.605 ± 0.389
3.964IleVal: 3.964 ± 0.369
0.823IleTrp: 0.823 ± 0.165
2.44IleTyr: 2.44 ± 0.28
0.0IleXaa: 0.0 ± 0.0
Lys
6.434LysAla: 6.434 ± 0.48
0.793LysCys: 0.793 ± 0.228
5.001LysAsp: 5.001 ± 0.383
4.757LysGlu: 4.757 ± 0.382
3.08LysPhe: 3.08 ± 0.355
3.293LysGly: 3.293 ± 0.321
1.22LysHis: 1.22 ± 0.227
3.812LysIle: 3.812 ± 0.339
4.879LysLys: 4.879 ± 0.518
6.77LysLeu: 6.77 ± 0.552
2.318LysMet: 2.318 ± 0.317
3.69LysAsn: 3.69 ± 0.412
2.775LysPro: 2.775 ± 0.333
3.324LysGln: 3.324 ± 0.333
3.476LysArg: 3.476 ± 0.333
4.666LysSer: 4.666 ± 0.42
4.33LysThr: 4.33 ± 0.418
4.727LysVal: 4.727 ± 0.425
0.762LysTrp: 0.762 ± 0.173
3.08LysTyr: 3.08 ± 0.326
0.0LysXaa: 0.0 ± 0.0
Leu
6.648LeuAla: 6.648 ± 0.476
0.793LeuCys: 0.793 ± 0.155
5.947LeuAsp: 5.947 ± 0.435
7.014LeuGlu: 7.014 ± 0.558
3.08LeuPhe: 3.08 ± 0.389
5.215LeuGly: 5.215 ± 0.45
1.86LeuHis: 1.86 ± 0.242
5.276LeuIle: 5.276 ± 0.433
6.099LeuLys: 6.099 ± 0.457
6.556LeuLeu: 6.556 ± 0.46
2.257LeuMet: 2.257 ± 0.315
5.184LeuAsn: 5.184 ± 0.426
3.507LeuPro: 3.507 ± 0.302
2.989LeuGln: 2.989 ± 0.37
4.239LeuArg: 4.239 ± 0.311
5.123LeuSer: 5.123 ± 0.45
4.513LeuThr: 4.513 ± 0.41
5.215LeuVal: 5.215 ± 0.371
0.671LeuTrp: 0.671 ± 0.131
2.806LeuTyr: 2.806 ± 0.313
0.0LeuXaa: 0.0 ± 0.0
Met
1.921MetAla: 1.921 ± 0.266
0.305MetCys: 0.305 ± 0.098
1.311MetAsp: 1.311 ± 0.207
2.013MetGlu: 2.013 ± 0.266
0.884MetPhe: 0.884 ± 0.174
1.494MetGly: 1.494 ± 0.235
0.427MetHis: 0.427 ± 0.108
1.921MetIle: 1.921 ± 0.272
2.226MetLys: 2.226 ± 0.321
2.257MetLeu: 2.257 ± 0.279
0.518MetMet: 0.518 ± 0.128
1.067MetAsn: 1.067 ± 0.18
0.854MetPro: 0.854 ± 0.146
1.22MetGln: 1.22 ± 0.259
1.189MetArg: 1.189 ± 0.165
2.257MetSer: 2.257 ± 0.298
1.952MetThr: 1.952 ± 0.238
1.525MetVal: 1.525 ± 0.236
0.152MetTrp: 0.152 ± 0.063
0.915MetTyr: 0.915 ± 0.148
0.0MetXaa: 0.0 ± 0.0
Asn
3.324AsnAla: 3.324 ± 0.486
0.579AsnCys: 0.579 ± 0.143
2.867AsnAsp: 2.867 ± 0.27
2.714AsnGlu: 2.714 ± 0.318
2.409AsnPhe: 2.409 ± 0.276
4.635AsnGly: 4.635 ± 0.429
0.732AsnHis: 0.732 ± 0.183
4.025AsnIle: 4.025 ± 0.361
3.995AsnLys: 3.995 ± 0.39
4.574AsnLeu: 4.574 ± 0.385
0.976AsnMet: 0.976 ± 0.18
3.781AsnAsn: 3.781 ± 0.382
2.836AsnPro: 2.836 ± 0.38
1.738AsnGln: 1.738 ± 0.269
2.684AsnArg: 2.684 ± 0.324
3.903AsnSer: 3.903 ± 0.337
3.202AsnThr: 3.202 ± 0.293
3.568AsnVal: 3.568 ± 0.294
0.457AsnTrp: 0.457 ± 0.142
1.921AsnTyr: 1.921 ± 0.235
0.0AsnXaa: 0.0 ± 0.0
Pro
2.897ProAla: 2.897 ± 0.341
0.427ProCys: 0.427 ± 0.114
2.47ProAsp: 2.47 ± 0.282
3.232ProGlu: 3.232 ± 0.391
1.525ProPhe: 1.525 ± 0.207
2.043ProGly: 2.043 ± 0.213
0.701ProHis: 0.701 ± 0.165
2.348ProIle: 2.348 ± 0.283
2.074ProLys: 2.074 ± 0.228
2.165ProLeu: 2.165 ± 0.287
0.518ProMet: 0.518 ± 0.133
2.074ProAsn: 2.074 ± 0.264
1.403ProPro: 1.403 ± 0.27
0.945ProGln: 0.945 ± 0.189
1.494ProArg: 1.494 ± 0.206
2.196ProSer: 2.196 ± 0.261
2.074ProThr: 2.074 ± 0.24
2.531ProVal: 2.531 ± 0.256
0.427ProTrp: 0.427 ± 0.103
1.708ProTyr: 1.708 ± 0.215
0.0ProXaa: 0.0 ± 0.0
Gln
2.501GlnAla: 2.501 ± 0.417
0.457GlnCys: 0.457 ± 0.129
2.043GlnAsp: 2.043 ± 0.254
2.714GlnGlu: 2.714 ± 0.341
1.433GlnPhe: 1.433 ± 0.237
1.799GlnGly: 1.799 ± 0.184
0.64GlnHis: 0.64 ± 0.163
2.501GlnIle: 2.501 ± 0.32
2.287GlnLys: 2.287 ± 0.311
3.995GlnLeu: 3.995 ± 0.441
1.159GlnMet: 1.159 ± 0.283
1.708GlnAsn: 1.708 ± 0.197
0.518GlnPro: 0.518 ± 0.143
2.531GlnGln: 2.531 ± 0.621
2.043GlnArg: 2.043 ± 0.3
2.257GlnSer: 2.257 ± 0.259
1.982GlnThr: 1.982 ± 0.269
2.714GlnVal: 2.714 ± 0.302
0.457GlnTrp: 0.457 ± 0.129
1.494GlnTyr: 1.494 ± 0.219
0.0GlnXaa: 0.0 ± 0.0
Arg
2.989ArgAla: 2.989 ± 0.289
0.396ArgCys: 0.396 ± 0.109
2.775ArgAsp: 2.775 ± 0.273
3.232ArgGlu: 3.232 ± 0.35
1.586ArgPhe: 1.586 ± 0.223
3.263ArgGly: 3.263 ± 0.321
0.701ArgHis: 0.701 ± 0.19
2.958ArgIle: 2.958 ± 0.33
3.598ArgLys: 3.598 ± 0.402
4.605ArgLeu: 4.605 ± 0.34
1.464ArgMet: 1.464 ± 0.222
2.745ArgAsn: 2.745 ± 0.278
1.403ArgPro: 1.403 ± 0.212
1.83ArgGln: 1.83 ± 0.255
2.165ArgArg: 2.165 ± 0.288
2.287ArgSer: 2.287 ± 0.251
2.562ArgThr: 2.562 ± 0.263
2.958ArgVal: 2.958 ± 0.259
0.457ArgTrp: 0.457 ± 0.125
1.921ArgTyr: 1.921 ± 0.305
0.0ArgXaa: 0.0 ± 0.0
Ser
4.483SerAla: 4.483 ± 0.627
0.854SerCys: 0.854 ± 0.162
3.476SerAsp: 3.476 ± 0.32
3.903SerGlu: 3.903 ± 0.347
3.385SerPhe: 3.385 ± 0.304
4.91SerGly: 4.91 ± 0.544
0.823SerHis: 0.823 ± 0.147
4.117SerIle: 4.117 ± 0.32
5.062SerLys: 5.062 ± 0.391
5.855SerLeu: 5.855 ± 0.386
1.83SerMet: 1.83 ± 0.24
3.812SerAsn: 3.812 ± 0.401
2.318SerPro: 2.318 ± 0.267
2.013SerGln: 2.013 ± 0.257
3.019SerArg: 3.019 ± 0.3
4.544SerSer: 4.544 ± 0.454
3.781SerThr: 3.781 ± 0.345
4.361SerVal: 4.361 ± 0.341
1.281SerTrp: 1.281 ± 0.195
2.348SerTyr: 2.348 ± 0.27
0.0SerXaa: 0.0 ± 0.0
Thr
4.361ThrAla: 4.361 ± 0.533
0.671ThrCys: 0.671 ± 0.133
2.867ThrAsp: 2.867 ± 0.317
3.537ThrGlu: 3.537 ± 0.297
2.47ThrPhe: 2.47 ± 0.279
4.788ThrGly: 4.788 ± 0.45
1.037ThrHis: 1.037 ± 0.203
4.3ThrIle: 4.3 ± 0.385
3.72ThrLys: 3.72 ± 0.348
4.391ThrLeu: 4.391 ± 0.382
1.403ThrMet: 1.403 ± 0.215
3.598ThrAsn: 3.598 ± 0.382
2.348ThrPro: 2.348 ± 0.267
2.318ThrGln: 2.318 ± 0.221
2.47ThrArg: 2.47 ± 0.249
4.147ThrSer: 4.147 ± 0.438
3.111ThrThr: 3.111 ± 0.324
4.056ThrVal: 4.056 ± 0.372
0.732ThrTrp: 0.732 ± 0.155
2.226ThrTyr: 2.226 ± 0.291
0.0ThrXaa: 0.0 ± 0.0
Val
4.91ValAla: 4.91 ± 0.44
0.549ValCys: 0.549 ± 0.147
4.361ValAsp: 4.361 ± 0.388
4.056ValGlu: 4.056 ± 0.391
2.592ValPhe: 2.592 ± 0.304
3.812ValGly: 3.812 ± 0.363
0.884ValHis: 0.884 ± 0.174
4.33ValIle: 4.33 ± 0.331
4.818ValLys: 4.818 ± 0.326
4.605ValLeu: 4.605 ± 0.362
1.494ValMet: 1.494 ± 0.174
3.263ValAsn: 3.263 ± 0.339
2.501ValPro: 2.501 ± 0.281
2.196ValGln: 2.196 ± 0.277
2.775ValArg: 2.775 ± 0.293
4.727ValSer: 4.727 ± 0.443
4.3ValThr: 4.3 ± 0.425
4.33ValVal: 4.33 ± 0.416
0.732ValTrp: 0.732 ± 0.165
2.592ValTyr: 2.592 ± 0.298
0.0ValXaa: 0.0 ± 0.0
Trp
0.427TrpAla: 0.427 ± 0.12
0.305TrpCys: 0.305 ± 0.085
0.823TrpAsp: 0.823 ± 0.146
1.128TrpGlu: 1.128 ± 0.175
0.61TrpPhe: 0.61 ± 0.143
0.579TrpGly: 0.579 ± 0.143
0.213TrpHis: 0.213 ± 0.078
0.64TrpIle: 0.64 ± 0.172
1.006TrpLys: 1.006 ± 0.16
1.189TrpLeu: 1.189 ± 0.2
0.366TrpMet: 0.366 ± 0.119
0.549TrpAsn: 0.549 ± 0.153
0.244TrpPro: 0.244 ± 0.095
0.518TrpGln: 0.518 ± 0.139
0.61TrpArg: 0.61 ± 0.115
0.884TrpSer: 0.884 ± 0.215
0.671TrpThr: 0.671 ± 0.15
0.915TrpVal: 0.915 ± 0.166
0.152TrpTrp: 0.152 ± 0.086
0.244TrpTyr: 0.244 ± 0.082
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.196TyrAla: 2.196 ± 0.264
0.762TyrCys: 0.762 ± 0.157
2.867TyrAsp: 2.867 ± 0.29
1.891TyrGlu: 1.891 ± 0.231
1.708TyrPhe: 1.708 ± 0.251
2.714TyrGly: 2.714 ± 0.281
0.854TyrHis: 0.854 ± 0.154
2.44TyrIle: 2.44 ± 0.32
3.172TyrLys: 3.172 ± 0.35
2.806TyrLeu: 2.806 ± 0.312
0.884TyrMet: 0.884 ± 0.149
2.531TyrAsn: 2.531 ± 0.304
1.433TyrPro: 1.433 ± 0.168
1.647TyrGln: 1.647 ± 0.244
1.555TyrArg: 1.555 ± 0.233
2.684TyrSer: 2.684 ± 0.242
2.562TyrThr: 2.562 ± 0.274
2.226TyrVal: 2.226 ± 0.26
0.64TyrTrp: 0.64 ± 0.153
1.708TyrTyr: 1.708 ± 0.232
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 163 proteins (32793 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski