Parasitella parasitica
Average proteome isoelectric point is 6.62
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 13357 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0B7N2A6|A0A0B7N2A6_9FUNG Uncharacterized protein (Fragment) OS=Parasitella parasitica OX=35722 GN=PARPA_03018.1 scaffold 6065 PE=4 SV=1
MM1 pKa = 7.71 ARR3 pKa = 11.84 LTQLCLATLALLVIATSAAPCHH25 pKa = 6.36 GDD27 pKa = 3.69 NGLGDD32 pKa = 4.28 HH33 pKa = 7.6 DD34 pKa = 5.24 GGDD37 pKa = 3.98 HH38 pKa = 6.6 EE39 pKa = 5.61 LGHH42 pKa = 7.24 HH43 pKa = 6.74 GDD45 pKa = 4.23 HH46 pKa = 7.24 EE47 pKa = 4.62 DD48 pKa = 4.26 AEE50 pKa = 4.51 DD51 pKa = 3.68 WDD53 pKa = 4.77 DD54 pKa = 3.74 EE55 pKa = 5.36 HH56 pKa = 9.23 DD57 pKa = 5.05 GEE59 pKa = 5.27 WDD61 pKa = 4.39 DD62 pKa = 5.69 DD63 pKa = 4.24 DD64 pKa = 7.07 DD65 pKa = 5.65 YY66 pKa = 12.09 EE67 pKa = 5.9 GGNDD71 pKa = 4.63 DD72 pKa = 5.42 EE73 pKa = 5.07 DD74 pKa = 4.11 WNDD77 pKa = 3.38 GGNGIDD83 pKa = 5.36 NEE85 pKa = 4.22 DD86 pKa = 3.51 WDD88 pKa = 4.09 NGEE91 pKa = 5.12 DD92 pKa = 4.53 NGGCHH97 pKa = 6.85 PRR99 pKa = 11.84 GCSDD103 pKa = 4.38 YY104 pKa = 11.65 DD105 pKa = 3.61 DD106 pKa = 6.21 DD107 pKa = 5.53 YY108 pKa = 11.87 PNGNNGGNHH117 pKa = 6.47 GGNNGGNHH125 pKa = 6.54 GGNNGGNNGGNHH137 pKa = 6.21 GNNGNNGNNGNNGGGRR153 pKa = 11.84 GNNDD157 pKa = 3.63 DD158 pKa = 4.79 NDD160 pKa = 4.48 DD161 pKa = 3.84 GSLIDD166 pKa = 4.16 VDD168 pKa = 4.25 AGHH171 pKa = 7.32 IGTGNLNFQNALQGVVNLDD190 pKa = 3.63 DD191 pKa = 5.83 LNANDD196 pKa = 4.03 IAHH199 pKa = 7.7 DD200 pKa = 3.83 INALNN205 pKa = 3.75
Molecular weight: 21.26 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.704
IPC2_protein 3.63
IPC_protein 3.681
Toseland 3.439
ProMoST 3.872
Dawson 3.706
Bjellqvist 3.859
Wikipedia 3.681
Rodwell 3.503
Grimsley 3.35
Solomon 3.694
Lehninger 3.656
Nozaki 3.808
DTASelect 4.126
Thurlkill 3.516
EMBOSS 3.681
Sillero 3.821
Patrickios 0.757
IPC_peptide 3.681
IPC2_peptide 3.783
IPC2.peptide.svr19 3.72
Protein with the highest isoelectric point:
>tr|A0A0B7N1A7|A0A0B7N1A7_9FUNG HTH_Tnp_Tc3_2 domain-containing protein OS=Parasitella parasitica OX=35722 GN=PARPA_02769.1 scaffold 5272 PE=4 SV=1
AA1 pKa = 7.53 QAQAQAQAQAQAQAQAQAQAQAQAQAQAQAQAQAQAQAQAQAQAQAQAQAQAQAQAQAQAQALAQNQSQAHH72 pKa = 4.82 MQQLQQQAVQSRR84 pKa = 11.84 ASTPSQSHH92 pKa = 5.84 MSTPTISHH100 pKa = 5.83 STPLQQSASLPRR112 pKa = 11.84 QSLALPGNGLKK123 pKa = 10.07 SQQQTPTQAQAQLSAQHH140 pKa = 6.12 MALLQQQQQQQAAMQSMGLPQRR162 pKa = 11.84 SVQTSVPPTSQISNMLPTNVNNNNMTQTPVMSSIQQTALQQALSNGNGQLNPQASLQRR220 pKa = 11.84 IQQLVATGAIPLATAQQLVSQLVNSGNLQRR250 pKa = 11.84 QQAAAQQQQALQQVQQQQQQQFNQQSPVQQRR281 pKa = 11.84 LQQQQQQQQQQQQQQQQQQQQQ302 pKa = 3.04
Molecular weight: 32.58 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.437
IPC2_protein 10.935
IPC_protein 12.544
Toseland 12.705
ProMoST 13.203
Dawson 12.705
Bjellqvist 12.705
Wikipedia 13.173
Rodwell 12.281
Grimsley 12.749
Solomon 13.203
Lehninger 13.1
Nozaki 12.705
DTASelect 12.705
Thurlkill 12.705
EMBOSS 13.203
Sillero 12.705
Patrickios 12.047
IPC_peptide 13.203
IPC2_peptide 12.193
IPC2.peptide.svr19 9.121
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
13357
0
13357
5787778
17
6128
433.3
48.75
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.089 ± 0.021
1.395 ± 0.009
5.839 ± 0.015
6.132 ± 0.021
3.973 ± 0.014
4.946 ± 0.019
2.557 ± 0.01
5.927 ± 0.016
6.313 ± 0.02
8.977 ± 0.023
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.373 ± 0.008
5.053 ± 0.014
4.867 ± 0.017
4.829 ± 0.021
4.977 ± 0.015
8.474 ± 0.027
6.059 ± 0.018
5.828 ± 0.015
1.108 ± 0.007
3.255 ± 0.011
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here