Bacillus phage BalMu-1

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; unclassified Myoviridae

Average proteome isoelectric point is 6.54

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 56 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0N7ACI5|A0A0N7ACI5_9CAUD Uncharacterized protein OS=Bacillus phage BalMu-1 OX=1567487 GN=BalMu1_A27 PE=4 SV=1
MM1 pKa = 7.36NVIEE5 pKa = 4.62KK6 pKa = 11.02NEE8 pKa = 3.89GQKK11 pKa = 9.94IDD13 pKa = 3.88FEE15 pKa = 5.01VISGSLVSFRR25 pKa = 11.84GEE27 pKa = 3.86LFLDD31 pKa = 3.6LSRR34 pKa = 11.84YY35 pKa = 8.35EE36 pKa = 3.9RR37 pKa = 11.84DD38 pKa = 3.13YY39 pKa = 11.75DD40 pKa = 3.71VTIDD44 pKa = 3.06ISEE47 pKa = 4.1NHH49 pKa = 6.6FGMLMMGVSNRR60 pKa = 11.84YY61 pKa = 8.02VAQIEE66 pKa = 4.41IPACEE71 pKa = 4.14YY72 pKa = 10.97EE73 pKa = 4.29EE74 pKa = 4.68VEE76 pKa = 4.28IPEE79 pKa = 4.29EE80 pKa = 4.26EE81 pKa = 4.09IDD83 pKa = 4.48DD84 pKa = 4.46DD85 pKa = 4.76EE86 pKa = 4.99EE87 pKa = 6.31GSDD90 pKa = 3.57RR91 pKa = 11.84TKK93 pKa = 10.87LVPKK97 pKa = 10.3PFDD100 pKa = 3.79LEE102 pKa = 4.28NVTLVLWGIEE112 pKa = 3.9

Molecular weight: 12.94 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0N7AEG5|A0A0N7AEG5_9CAUD Uncharacterized protein OS=Bacillus phage BalMu-1 OX=1567487 GN=BalMu1_A52 PE=4 SV=1
MM1 pKa = 7.56RR2 pKa = 11.84AGVFALNLNNARR14 pKa = 11.84SNSNSNIGFRR24 pKa = 11.84SAAYY28 pKa = 8.98HH29 pKa = 4.86CQKK32 pKa = 10.65FKK34 pKa = 10.66VQRR37 pKa = 11.84TLFQHH42 pKa = 6.37GG43 pKa = 3.35

Molecular weight: 4.86 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

56

0

56

12149

39

1441

216.9

24.48

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.186 ± 0.477

0.527 ± 0.094

5.622 ± 0.188

8.536 ± 0.438

3.539 ± 0.143

7.112 ± 0.378

1.704 ± 0.166

6.182 ± 0.214

7.729 ± 0.329

8.215 ± 0.262

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.659 ± 0.127

4.445 ± 0.235

3.334 ± 0.162

3.712 ± 0.168

5.605 ± 0.327

6.601 ± 0.272

5.268 ± 0.187

7.161 ± 0.261

1.778 ± 0.378

3.087 ± 0.236
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski