Amino acid dipepetide frequency for Bacillus phage BalMu-1

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
5.021AlaAla: 5.021 ± 0.625
0.329AlaCys: 0.329 ± 0.136
2.963AlaAsp: 2.963 ± 0.473
5.762AlaGlu: 5.762 ± 0.804
2.799AlaPhe: 2.799 ± 0.45
4.692AlaGly: 4.692 ± 0.717
1.07AlaHis: 1.07 ± 0.328
5.515AlaIle: 5.515 ± 0.662
5.762AlaLys: 5.762 ± 0.64
7.244AlaLeu: 7.244 ± 0.725
1.811AlaMet: 1.811 ± 0.292
3.375AlaAsn: 3.375 ± 0.462
1.399AlaPro: 1.399 ± 0.273
1.893AlaGln: 1.893 ± 0.385
4.116AlaArg: 4.116 ± 0.545
5.186AlaSer: 5.186 ± 1.239
4.198AlaThr: 4.198 ± 0.901
4.774AlaVal: 4.774 ± 0.851
0.988AlaTrp: 0.988 ± 0.305
2.963AlaTyr: 2.963 ± 0.482
0.0AlaXaa: 0.0 ± 0.0
Cys
0.247CysAla: 0.247 ± 0.133
0.0CysCys: 0.0 ± 0.0
0.412CysAsp: 0.412 ± 0.163
0.823CysGlu: 0.823 ± 0.286
0.329CysPhe: 0.329 ± 0.154
0.329CysGly: 0.329 ± 0.146
0.247CysHis: 0.247 ± 0.124
0.247CysIle: 0.247 ± 0.132
0.329CysLys: 0.329 ± 0.191
0.329CysLeu: 0.329 ± 0.163
0.0CysMet: 0.0 ± 0.0
0.165CysAsn: 0.165 ± 0.108
0.247CysPro: 0.247 ± 0.179
0.165CysGln: 0.165 ± 0.126
0.412CysArg: 0.412 ± 0.172
0.329CysSer: 0.329 ± 0.129
0.082CysThr: 0.082 ± 0.081
0.247CysVal: 0.247 ± 0.139
0.247CysTrp: 0.247 ± 0.126
0.082CysTyr: 0.082 ± 0.086
0.0CysXaa: 0.0 ± 0.0
Asp
4.61AspAla: 4.61 ± 0.625
0.494AspCys: 0.494 ± 0.214
4.116AspAsp: 4.116 ± 0.446
5.351AspGlu: 5.351 ± 0.986
2.799AspPhe: 2.799 ± 0.492
3.457AspGly: 3.457 ± 0.579
0.576AspHis: 0.576 ± 0.21
3.869AspIle: 3.869 ± 0.556
3.622AspLys: 3.622 ± 0.624
4.61AspLeu: 4.61 ± 0.568
1.399AspMet: 1.399 ± 0.332
1.893AspAsn: 1.893 ± 0.38
1.976AspPro: 1.976 ± 0.41
1.235AspGln: 1.235 ± 0.341
3.128AspArg: 3.128 ± 0.54
3.951AspSer: 3.951 ± 0.766
2.14AspThr: 2.14 ± 0.384
3.704AspVal: 3.704 ± 0.488
1.235AspTrp: 1.235 ± 0.519
2.058AspTyr: 2.058 ± 0.349
0.0AspXaa: 0.0 ± 0.0
Glu
5.762GluAla: 5.762 ± 0.7
0.494GluCys: 0.494 ± 0.193
4.857GluAsp: 4.857 ± 0.812
7.491GluGlu: 7.491 ± 1.009
2.963GluPhe: 2.963 ± 0.513
5.68GluGly: 5.68 ± 0.813
1.152GluHis: 1.152 ± 0.285
5.433GluIle: 5.433 ± 0.639
6.092GluLys: 6.092 ± 0.839
8.149GluLeu: 8.149 ± 0.966
2.963GluMet: 2.963 ± 0.628
4.116GluAsn: 4.116 ± 0.57
2.881GluPro: 2.881 ± 0.501
3.869GluGln: 3.869 ± 0.559
4.198GluArg: 4.198 ± 0.729
4.281GluSer: 4.281 ± 0.661
4.527GluThr: 4.527 ± 0.705
6.668GluVal: 6.668 ± 0.94
1.564GluTrp: 1.564 ± 0.405
2.223GluTyr: 2.223 ± 0.428
0.0GluXaa: 0.0 ± 0.0
Phe
3.375PheAla: 3.375 ± 0.515
0.247PheCys: 0.247 ± 0.135
1.976PheAsp: 1.976 ± 0.404
3.457PheGlu: 3.457 ± 0.514
0.823PhePhe: 0.823 ± 0.274
3.128PheGly: 3.128 ± 0.478
0.329PheHis: 0.329 ± 0.162
1.729PheIle: 1.729 ± 0.414
2.552PheLys: 2.552 ± 0.469
2.552PheLeu: 2.552 ± 0.413
0.741PheMet: 0.741 ± 0.234
1.893PheAsn: 1.893 ± 0.477
1.317PhePro: 1.317 ± 0.261
0.988PheGln: 0.988 ± 0.244
2.223PheArg: 2.223 ± 0.479
2.552PheSer: 2.552 ± 0.727
1.564PheThr: 1.564 ± 0.376
2.14PheVal: 2.14 ± 0.442
0.576PheTrp: 0.576 ± 0.204
1.235PheTyr: 1.235 ± 0.315
0.0PheXaa: 0.0 ± 0.0
Gly
5.351GlyAla: 5.351 ± 0.822
0.165GlyCys: 0.165 ± 0.104
4.857GlyAsp: 4.857 ± 0.718
6.338GlyGlu: 6.338 ± 0.86
2.552GlyPhe: 2.552 ± 0.317
5.845GlyGly: 5.845 ± 0.914
1.976GlyHis: 1.976 ± 0.357
4.281GlyIle: 4.281 ± 0.678
5.104GlyLys: 5.104 ± 1.098
4.692GlyLeu: 4.692 ± 0.63
2.058GlyMet: 2.058 ± 0.338
2.47GlyAsn: 2.47 ± 0.384
0.988GlyPro: 0.988 ± 0.197
2.963GlyGln: 2.963 ± 0.478
3.622GlyArg: 3.622 ± 0.63
4.445GlySer: 4.445 ± 0.654
3.951GlyThr: 3.951 ± 0.567
5.598GlyVal: 5.598 ± 0.45
1.811GlyTrp: 1.811 ± 0.669
1.976GlyTyr: 1.976 ± 0.406
0.0GlyXaa: 0.0 ± 0.0
His
1.646HisAla: 1.646 ± 0.507
0.165HisCys: 0.165 ± 0.113
0.576HisAsp: 0.576 ± 0.225
0.905HisGlu: 0.905 ± 0.332
0.823HisPhe: 0.823 ± 0.262
1.152HisGly: 1.152 ± 0.318
0.494HisHis: 0.494 ± 0.201
1.07HisIle: 1.07 ± 0.285
1.564HisLys: 1.564 ± 0.379
1.893HisLeu: 1.893 ± 0.355
0.412HisMet: 0.412 ± 0.183
0.741HisAsn: 0.741 ± 0.232
0.823HisPro: 0.823 ± 0.305
0.494HisGln: 0.494 ± 0.249
0.165HisArg: 0.165 ± 0.114
0.905HisSer: 0.905 ± 0.373
0.988HisThr: 0.988 ± 0.281
1.152HisVal: 1.152 ± 0.365
0.741HisTrp: 0.741 ± 0.43
0.329HisTyr: 0.329 ± 0.173
0.0HisXaa: 0.0 ± 0.0
Ile
3.375IleAla: 3.375 ± 0.401
0.741IleCys: 0.741 ± 0.257
3.622IleAsp: 3.622 ± 0.616
6.668IleGlu: 6.668 ± 1.022
2.058IlePhe: 2.058 ± 0.244
4.61IleGly: 4.61 ± 0.501
0.905IleHis: 0.905 ± 0.319
3.375IleIle: 3.375 ± 0.502
5.186IleLys: 5.186 ± 0.596
3.869IleLeu: 3.869 ± 0.515
1.646IleMet: 1.646 ± 0.285
2.552IleAsn: 2.552 ± 0.438
2.47IlePro: 2.47 ± 0.466
1.317IleGln: 1.317 ± 0.343
3.951IleArg: 3.951 ± 0.538
4.445IleSer: 4.445 ± 0.516
3.54IleThr: 3.54 ± 0.598
3.704IleVal: 3.704 ± 0.605
1.317IleTrp: 1.317 ± 0.469
2.47IleTyr: 2.47 ± 0.554
0.0IleXaa: 0.0 ± 0.0
Lys
6.338LysAla: 6.338 ± 0.749
0.247LysCys: 0.247 ± 0.13
2.881LysAsp: 2.881 ± 0.492
5.845LysGlu: 5.845 ± 0.684
1.729LysPhe: 1.729 ± 0.42
5.104LysGly: 5.104 ± 0.758
1.317LysHis: 1.317 ± 0.283
4.198LysIle: 4.198 ± 0.661
7.162LysLys: 7.162 ± 1.232
6.585LysLeu: 6.585 ± 0.81
2.634LysMet: 2.634 ± 0.613
3.54LysAsn: 3.54 ± 0.562
2.387LysPro: 2.387 ± 0.495
4.281LysGln: 4.281 ± 0.571
5.104LysArg: 5.104 ± 0.735
4.527LysSer: 4.527 ± 0.799
3.951LysThr: 3.951 ± 0.585
5.845LysVal: 5.845 ± 0.73
1.646LysTrp: 1.646 ± 0.625
1.976LysTyr: 1.976 ± 0.471
0.0LysXaa: 0.0 ± 0.0
Leu
5.762LeuAla: 5.762 ± 0.727
0.412LeuCys: 0.412 ± 0.187
5.515LeuAsp: 5.515 ± 0.526
6.256LeuGlu: 6.256 ± 0.643
3.293LeuPhe: 3.293 ± 0.518
5.598LeuGly: 5.598 ± 1.094
0.988LeuHis: 0.988 ± 0.357
3.704LeuIle: 3.704 ± 0.605
6.421LeuLys: 6.421 ± 0.986
5.762LeuLeu: 5.762 ± 0.805
2.223LeuMet: 2.223 ± 0.542
4.363LeuAsn: 4.363 ± 0.689
2.552LeuPro: 2.552 ± 0.534
2.881LeuGln: 2.881 ± 0.542
5.762LeuArg: 5.762 ± 0.844
8.149LeuSer: 8.149 ± 1.042
4.363LeuThr: 4.363 ± 0.628
4.774LeuVal: 4.774 ± 0.627
1.317LeuTrp: 1.317 ± 0.349
2.058LeuTyr: 2.058 ± 0.389
0.0LeuXaa: 0.0 ± 0.0
Met
1.646MetAla: 1.646 ± 0.321
0.082MetCys: 0.082 ± 0.086
1.729MetAsp: 1.729 ± 0.395
1.399MetGlu: 1.399 ± 0.382
0.576MetPhe: 0.576 ± 0.198
1.399MetGly: 1.399 ± 0.421
0.329MetHis: 0.329 ± 0.159
1.729MetIle: 1.729 ± 0.345
3.128MetLys: 3.128 ± 0.593
2.881MetLeu: 2.881 ± 0.457
0.823MetMet: 0.823 ± 0.234
1.646MetAsn: 1.646 ± 0.372
0.823MetPro: 0.823 ± 0.255
1.152MetGln: 1.152 ± 0.263
1.729MetArg: 1.729 ± 0.362
1.482MetSer: 1.482 ± 0.316
1.976MetThr: 1.976 ± 0.443
1.152MetVal: 1.152 ± 0.327
0.165MetTrp: 0.165 ± 0.106
0.741MetTyr: 0.741 ± 0.239
0.0MetXaa: 0.0 ± 0.0
Asn
3.704AsnAla: 3.704 ± 0.659
0.082AsnCys: 0.082 ± 0.082
2.14AsnAsp: 2.14 ± 0.437
4.527AsnGlu: 4.527 ± 0.663
1.646AsnPhe: 1.646 ± 0.364
4.527AsnGly: 4.527 ± 0.598
0.741AsnHis: 0.741 ± 0.257
1.811AsnIle: 1.811 ± 0.461
2.881AsnLys: 2.881 ± 0.478
3.787AsnLeu: 3.787 ± 0.717
0.905AsnMet: 0.905 ± 0.319
2.634AsnAsn: 2.634 ± 0.517
1.399AsnPro: 1.399 ± 0.452
1.482AsnGln: 1.482 ± 0.345
2.634AsnArg: 2.634 ± 0.482
2.223AsnSer: 2.223 ± 0.398
1.235AsnThr: 1.235 ± 0.336
4.281AsnVal: 4.281 ± 0.567
0.741AsnTrp: 0.741 ± 0.244
1.07AsnTyr: 1.07 ± 0.29
0.0AsnXaa: 0.0 ± 0.0
Pro
1.482ProAla: 1.482 ± 0.347
0.082ProCys: 0.082 ± 0.069
1.893ProAsp: 1.893 ± 0.406
3.046ProGlu: 3.046 ± 0.506
1.564ProPhe: 1.564 ± 0.289
1.482ProGly: 1.482 ± 0.32
0.576ProHis: 0.576 ± 0.204
2.716ProIle: 2.716 ± 0.567
2.634ProLys: 2.634 ± 0.465
3.375ProLeu: 3.375 ± 0.438
0.659ProMet: 0.659 ± 0.197
2.058ProAsn: 2.058 ± 0.323
1.564ProPro: 1.564 ± 0.436
1.07ProGln: 1.07 ± 0.298
1.317ProArg: 1.317 ± 0.326
2.223ProSer: 2.223 ± 0.444
2.305ProThr: 2.305 ± 0.466
1.729ProVal: 1.729 ± 0.438
0.412ProTrp: 0.412 ± 0.227
1.152ProTyr: 1.152 ± 0.345
0.0ProXaa: 0.0 ± 0.0
Gln
2.47GlnAla: 2.47 ± 0.357
0.412GlnCys: 0.412 ± 0.184
1.811GlnAsp: 1.811 ± 0.384
3.128GlnGlu: 3.128 ± 0.623
1.07GlnPhe: 1.07 ± 0.328
2.799GlnGly: 2.799 ± 0.649
0.823GlnHis: 0.823 ± 0.278
2.387GlnIle: 2.387 ± 0.52
2.963GlnLys: 2.963 ± 0.392
2.963GlnLeu: 2.963 ± 0.573
0.988GlnMet: 0.988 ± 0.268
1.729GlnAsn: 1.729 ± 0.34
1.07GlnPro: 1.07 ± 0.313
1.811GlnGln: 1.811 ± 0.42
2.305GlnArg: 2.305 ± 0.365
1.811GlnSer: 1.811 ± 0.402
1.729GlnThr: 1.729 ± 0.373
2.881GlnVal: 2.881 ± 0.571
0.905GlnTrp: 0.905 ± 0.327
1.07GlnTyr: 1.07 ± 0.325
0.0GlnXaa: 0.0 ± 0.0
Arg
3.787ArgAla: 3.787 ± 0.596
0.082ArgCys: 0.082 ± 0.084
2.47ArgAsp: 2.47 ± 0.472
4.034ArgGlu: 4.034 ± 0.811
1.893ArgPhe: 1.893 ± 0.382
3.293ArgGly: 3.293 ± 0.403
0.905ArgHis: 0.905 ± 0.251
4.445ArgIle: 4.445 ± 0.428
5.186ArgLys: 5.186 ± 0.947
5.104ArgLeu: 5.104 ± 0.647
1.811ArgMet: 1.811 ± 0.341
1.317ArgAsn: 1.317 ± 0.321
2.963ArgPro: 2.963 ± 0.558
2.552ArgGln: 2.552 ± 0.559
2.552ArgArg: 2.552 ± 0.505
3.046ArgSer: 3.046 ± 0.47
3.375ArgThr: 3.375 ± 0.692
4.116ArgVal: 4.116 ± 0.778
0.905ArgTrp: 0.905 ± 0.201
2.223ArgTyr: 2.223 ± 0.484
0.0ArgXaa: 0.0 ± 0.0
Ser
4.61SerAla: 4.61 ± 1.328
0.165SerCys: 0.165 ± 0.125
4.527SerAsp: 4.527 ± 0.536
5.351SerGlu: 5.351 ± 0.564
2.387SerPhe: 2.387 ± 0.385
5.762SerGly: 5.762 ± 0.676
1.399SerHis: 1.399 ± 0.365
4.034SerIle: 4.034 ± 0.491
4.527SerLys: 4.527 ± 0.673
4.939SerLeu: 4.939 ± 0.589
1.646SerMet: 1.646 ± 0.461
3.21SerAsn: 3.21 ± 0.594
2.305SerPro: 2.305 ± 0.348
2.963SerGln: 2.963 ± 0.376
3.54SerArg: 3.54 ± 0.434
5.186SerSer: 5.186 ± 0.606
2.799SerThr: 2.799 ± 0.498
3.869SerVal: 3.869 ± 0.613
0.494SerTrp: 0.494 ± 0.284
2.305SerTyr: 2.305 ± 0.426
0.0SerXaa: 0.0 ± 0.0
Thr
3.622ThrAla: 3.622 ± 0.63
0.082ThrCys: 0.082 ± 0.07
2.223ThrAsp: 2.223 ± 0.366
3.951ThrGlu: 3.951 ± 0.621
1.811ThrPhe: 1.811 ± 0.418
3.293ThrGly: 3.293 ± 0.498
1.235ThrHis: 1.235 ± 0.315
4.116ThrIle: 4.116 ± 0.688
3.951ThrLys: 3.951 ± 0.581
5.515ThrLeu: 5.515 ± 0.648
1.07ThrMet: 1.07 ± 0.272
1.399ThrAsn: 1.399 ± 0.408
2.716ThrPro: 2.716 ± 0.444
0.905ThrGln: 0.905 ± 0.29
2.305ThrArg: 2.305 ± 0.517
2.963ThrSer: 2.963 ± 0.37
3.046ThrThr: 3.046 ± 0.403
6.009ThrVal: 6.009 ± 1.0
0.905ThrTrp: 0.905 ± 0.292
1.564ThrTyr: 1.564 ± 0.367
0.0ThrXaa: 0.0 ± 0.0
Val
5.68ValAla: 5.68 ± 1.007
0.494ValCys: 0.494 ± 0.261
3.951ValAsp: 3.951 ± 0.544
5.845ValGlu: 5.845 ± 0.77
2.223ValPhe: 2.223 ± 0.453
5.845ValGly: 5.845 ± 1.006
0.905ValHis: 0.905 ± 0.306
4.61ValIle: 4.61 ± 0.571
5.104ValLys: 5.104 ± 0.551
4.363ValLeu: 4.363 ± 0.535
1.482ValMet: 1.482 ± 0.294
3.787ValAsn: 3.787 ± 0.574
2.14ValPro: 2.14 ± 0.369
2.881ValGln: 2.881 ± 0.405
4.034ValArg: 4.034 ± 0.674
5.845ValSer: 5.845 ± 0.82
4.527ValThr: 4.527 ± 0.843
5.351ValVal: 5.351 ± 0.835
0.576ValTrp: 0.576 ± 0.255
1.976ValTyr: 1.976 ± 0.373
0.0ValXaa: 0.0 ± 0.0
Trp
1.646TrpAla: 1.646 ± 0.446
0.082TrpCys: 0.082 ± 0.086
2.14TrpAsp: 2.14 ± 1.201
2.14TrpGlu: 2.14 ± 0.717
0.823TrpPhe: 0.823 ± 0.301
0.988TrpGly: 0.988 ± 0.259
0.247TrpHis: 0.247 ± 0.12
0.741TrpIle: 0.741 ± 0.231
0.741TrpLys: 0.741 ± 0.21
1.317TrpLeu: 1.317 ± 0.416
0.412TrpMet: 0.412 ± 0.148
0.659TrpAsn: 0.659 ± 0.262
0.165TrpPro: 0.165 ± 0.125
0.988TrpGln: 0.988 ± 0.363
0.576TrpArg: 0.576 ± 0.19
1.152TrpSer: 1.152 ± 0.354
0.494TrpThr: 0.494 ± 0.198
0.905TrpVal: 0.905 ± 0.261
0.988TrpTrp: 0.988 ± 0.892
0.576TrpTyr: 0.576 ± 0.2
0.0TrpXaa: 0.0 ± 0.0
Tyr
1.729TyrAla: 1.729 ± 0.292
0.412TyrCys: 0.412 ± 0.189
1.564TyrAsp: 1.564 ± 0.277
3.046TyrGlu: 3.046 ± 0.48
1.235TyrPhe: 1.235 ± 0.382
2.14TyrGly: 2.14 ± 0.375
0.823TyrHis: 0.823 ± 0.337
1.811TyrIle: 1.811 ± 0.451
2.387TyrLys: 2.387 ± 0.391
2.223TyrLeu: 2.223 ± 0.464
0.823TyrMet: 0.823 ± 0.259
0.905TyrAsn: 0.905 ± 0.218
1.152TyrPro: 1.152 ± 0.265
1.235TyrGln: 1.235 ± 0.296
2.387TyrArg: 2.387 ± 0.385
1.317TyrSer: 1.317 ± 0.294
1.893TyrThr: 1.893 ± 0.317
2.716TyrVal: 2.716 ± 0.498
0.247TyrTrp: 0.247 ± 0.134
0.823TyrTyr: 0.823 ± 0.302
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 56 proteins (12149 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski