[Candida] pseudohaemulonii

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Ascomycota; saccharomyceta; Saccharomycotina; Saccharomycetes; Saccharomycetales; Metschnikowiaceae; Clavispora;

Average proteome isoelectric point is 6.23

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5132 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2P7YNR6|A0A2P7YNR6_9ASCO Aminotran_1_2 domain-containing protein OS=[Candida] pseudohaemulonii OX=418784 GN=C7M61_003320 PE=3 SV=1
MM1 pKa = 7.43LAYY4 pKa = 10.19SVALFAYY11 pKa = 10.02AAVALGAQISGVFTSIDD28 pKa = 3.91SILAPANNRR37 pKa = 11.84PVTPSWQATVLWLMPRR53 pKa = 11.84LKK55 pKa = 10.51NIKK58 pKa = 10.43NGDD61 pKa = 3.65TFTLHH66 pKa = 5.54MPYY69 pKa = 10.31VFKK72 pKa = 10.02FTSLSNTLALTAGGTTFANCILFSGDD98 pKa = 3.14NVVGYY103 pKa = 10.18SEE105 pKa = 4.52VQCTATSAAEE115 pKa = 4.1NVEE118 pKa = 4.35SATGQITFPFTFNSGYY134 pKa = 10.82SLDD137 pKa = 4.01PVNLQAANAWNAGSNTVTWTDD158 pKa = 3.18GNTQISTTVNFLGGASQVILGSAEE182 pKa = 3.64NGVYY186 pKa = 10.77GLRR189 pKa = 11.84KK190 pKa = 8.86MVNLNVNQHH199 pKa = 5.57YY200 pKa = 10.72LLGPLCSSTMSGTLQITNPSSQVPFDD226 pKa = 4.06CLTIAGAISDD236 pKa = 4.89QINAWYY242 pKa = 10.0YY243 pKa = 8.09PQTANTVGVSVSSCSSTGAVVSFSNVPAGFRR274 pKa = 11.84PYY276 pKa = 10.24MNVNAAIPLGTFSSQNQYY294 pKa = 11.03SYY296 pKa = 11.57SFSCGWRR303 pKa = 11.84TLSGLITQLWTMYY316 pKa = 10.96SDD318 pKa = 4.05GNTGSGGAFKK328 pKa = 10.41PIVLTTVTDD337 pKa = 4.02PTATTTGVGTVTGASTNTIVVTVPISQTTITVTGTGTASTTHH379 pKa = 6.59SSTSSGTRR387 pKa = 11.84VISIDD392 pKa = 3.48VPTPTTTITRR402 pKa = 11.84TYY404 pKa = 10.32SGSTSSFLTVPATTGGTGTVIVDD427 pKa = 3.47VPTPTTTLTQTWTGTTTSTQTIPATSGGTATVIVDD462 pKa = 3.39VPTPVTTVTSTWTGLTTATVTQPVTSGGTQTVVVEE497 pKa = 4.57VPTPTTTLYY506 pKa = 9.46STWTGTVTTTTTVPATSGGTATVIVEE532 pKa = 4.27VPTPVTVITSTWTGSTTNVVTEE554 pKa = 4.16PFSLGTQTIVVEE566 pKa = 4.68VPTPTTTLTRR576 pKa = 11.84TWTGTEE582 pKa = 4.15TTTEE586 pKa = 4.35TIPAQSGGTGTVIVDD601 pKa = 3.27VPTPYY606 pKa = 8.22TTITRR611 pKa = 11.84TYY613 pKa = 8.32TGSVTTTEE621 pKa = 4.72TIPATTGGTGTVVVDD636 pKa = 3.64VPTPTTTITRR646 pKa = 11.84TWTGSTITTEE656 pKa = 4.25TVPATTGGTATLIVDD671 pKa = 4.23VPTPTTTVTSTYY683 pKa = 9.68TGLITTTVTVPATTGGTATVVVEE706 pKa = 4.52VPTPTTTITTTGDD719 pKa = 2.98VTNIVTLTILAASGGTEE736 pKa = 3.9TVVVEE741 pKa = 4.53VPTPTIYY748 pKa = 7.5TTRR751 pKa = 11.84TWTGSVTTTEE761 pKa = 4.72TIPATTGGTGTVIIDD776 pKa = 3.59VPTPTTTITRR786 pKa = 11.84TWTGSEE792 pKa = 4.17TTTEE796 pKa = 4.19TVPAASGGTGTVIVDD811 pKa = 3.48VPTPLTTITRR821 pKa = 11.84TWTGSITTTEE831 pKa = 4.4TIPAGSDD838 pKa = 3.11GTEE841 pKa = 4.09TIVVDD846 pKa = 4.03VPTPTTTITRR856 pKa = 11.84TWTGTEE862 pKa = 4.16STTEE866 pKa = 4.11TIPAQSGGTGTVIVDD881 pKa = 3.27VPTPYY886 pKa = 8.18TTITRR891 pKa = 11.84TWTGTTTQTEE901 pKa = 4.48TEE903 pKa = 4.16PAPSGGTGTVVIDD916 pKa = 3.7VPTPTTTITRR926 pKa = 11.84TWTGTEE932 pKa = 4.15TTTEE936 pKa = 4.36TIPAGSGGTEE946 pKa = 4.02TIIVDD951 pKa = 3.66VPTPYY956 pKa = 7.94TTLTRR961 pKa = 11.84TWTGTTTQTEE971 pKa = 4.48TEE973 pKa = 4.16PAPSGGTGTVVIDD986 pKa = 3.7VPTPTTTIYY995 pKa = 9.47STWTGSTISTRR1006 pKa = 11.84TIPASSGGTDD1016 pKa = 3.06TVVIEE1021 pKa = 4.6VPTPITVITSTWTGSITEE1039 pKa = 4.53VVTEE1043 pKa = 4.49PYY1045 pKa = 10.61EE1046 pKa = 4.65SGTQTIVVNVPTPTTTITRR1065 pKa = 11.84TWTGTEE1071 pKa = 4.11TTTEE1075 pKa = 4.15TVPAEE1080 pKa = 4.12SGGTGTVIIDD1090 pKa = 3.39VPTPYY1095 pKa = 7.93TTLTRR1100 pKa = 11.84TWTGTTTQTEE1110 pKa = 4.48TEE1112 pKa = 4.16PAPSGGTGTVIIDD1125 pKa = 3.59VPTPTTTITRR1135 pKa = 11.84TWTGTEE1141 pKa = 4.15TTTEE1145 pKa = 4.36TIPAGSGGTEE1155 pKa = 4.05TIVVDD1160 pKa = 3.87VPTPYY1165 pKa = 8.18TTITRR1170 pKa = 11.84TWTGTTTQTEE1180 pKa = 4.48TEE1182 pKa = 4.16PAPSGGTGTVVVDD1195 pKa = 3.48VPTPVTSITRR1205 pKa = 11.84TWTGTTTQTEE1215 pKa = 4.69TIPAQSGGTEE1225 pKa = 4.01TVIIDD1230 pKa = 3.58VPTPVTTLTRR1240 pKa = 11.84TWTDD1244 pKa = 3.1SITTTEE1250 pKa = 4.43TIPAEE1255 pKa = 4.22SGGTEE1260 pKa = 3.98TVIIDD1265 pKa = 3.73IPTPTTTLFSTWTGSTISTRR1285 pKa = 11.84TIPAEE1290 pKa = 4.02SGGTDD1295 pKa = 3.08TVVIEE1300 pKa = 4.6VPTPITVITSTWTGSITEE1318 pKa = 4.56IVTEE1322 pKa = 4.52PYY1324 pKa = 10.62EE1325 pKa = 4.7SGTQTIVVNVPTPVTTVTRR1344 pKa = 11.84TWTGTTTSTEE1354 pKa = 4.26TIPAEE1359 pKa = 4.17SGGTEE1364 pKa = 3.92TVIIDD1369 pKa = 3.58VPTPVTTITRR1379 pKa = 11.84TWTGTTTSTEE1389 pKa = 4.22TVPAEE1394 pKa = 4.1SGGTEE1399 pKa = 3.98TVIIDD1404 pKa = 3.44IPTPVTTITRR1414 pKa = 11.84TWTGTEE1420 pKa = 4.16STTEE1424 pKa = 4.12TIPAGSDD1431 pKa = 3.07GTEE1434 pKa = 3.9TVVVDD1439 pKa = 3.61VPTPYY1444 pKa = 8.15TTITRR1449 pKa = 11.84PWTGTFTTTEE1459 pKa = 4.15TEE1461 pKa = 4.07PAPSGGTGTVVVDD1474 pKa = 3.71VPYY1477 pKa = 10.71EE1478 pKa = 4.06ATTVTSTWTGTTTRR1492 pKa = 11.84TVSEE1496 pKa = 4.61PPAGSDD1502 pKa = 3.22TVGTVIIEE1510 pKa = 4.21VPPATTDD1517 pKa = 3.43YY1518 pKa = 7.55TTLTSTWTGTTTNTITNPPSGSDD1541 pKa = 3.2TVGTVVIEE1549 pKa = 4.12VPPTSSQFTTLTSTWTGTTTRR1570 pKa = 11.84TVTEE1574 pKa = 4.58PPSGSDD1580 pKa = 3.05TVGTVVIEE1588 pKa = 4.13VPPTSVDD1595 pKa = 3.44YY1596 pKa = 8.11TTVTSTWTGTTTATVTNPPSGSDD1619 pKa = 3.31TIGTVVIEE1627 pKa = 4.2VPPTSVDD1634 pKa = 3.44YY1635 pKa = 8.11TTVTSTWTGTTTATVTNPPSGSDD1658 pKa = 3.31TIGTVVIEE1666 pKa = 4.34VPPTSADD1673 pKa = 3.5YY1674 pKa = 7.52TTLTSTWTGTTTRR1687 pKa = 11.84TVTEE1691 pKa = 4.58PPSGSDD1697 pKa = 3.03TVGTVIIEE1705 pKa = 4.26VPPTTVPVIPPVLSWNWNSSYY1726 pKa = 10.87VVSLSSIPVPVSLSLSLTEE1745 pKa = 4.44SSEE1748 pKa = 4.15SSIASSIPPASGSEE1762 pKa = 3.92PATPAVSSSPASTEE1776 pKa = 3.88PPQLNTDD1783 pKa = 3.81SFSGPTSSGPITTASNQGPGPSPGPASDD1811 pKa = 3.87SSAPGPNPSGPNSGAEE1827 pKa = 4.27TSLSTASNPGPSPGPASEE1845 pKa = 4.33SSVPVSPGPNSEE1857 pKa = 4.31GSASTVTVVTTPGQPGPNPSGTGPVPSGPNSAGPSSSNPDD1897 pKa = 3.06TTVPVAPGPAPSGPAVPGTEE1917 pKa = 3.71SSAPGEE1923 pKa = 4.29SQPAPSGTANTEE1935 pKa = 4.14SQPSQTGPAPSGPNQTGPAPSGPNEE1960 pKa = 4.28TGSTPSGPNEE1970 pKa = 4.18NGPTSTGPAPANSAPPAPNPEE1991 pKa = 3.97PVASNTVPGSNPAPSGSNLGPAPSGPAPSGSNPGPVPVPGPAPSGTEE2038 pKa = 4.05SNNNPASTPYY2048 pKa = 7.94PTSVAGPSTRR2058 pKa = 11.84VPGNSAAASEE2068 pKa = 4.36TSSVVSEE2075 pKa = 4.81GPSSLVVCYY2084 pKa = 9.88KK2085 pKa = 10.29VAVSHH2090 pKa = 6.67ASSSSAPVGPEE2101 pKa = 3.74PSPASATPAISTYY2114 pKa = 10.85AGLAAAVKK2122 pKa = 10.06ISGLAAVVVPLLHH2135 pKa = 7.0VLFF2138 pKa = 5.26

Molecular weight: 215.84 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2P7YRU4|A0A2P7YRU4_9ASCO Uncharacterized protein OS=[Candida] pseudohaemulonii OX=418784 GN=C7M61_002605 PE=4 SV=1
MM1 pKa = 7.37FAGLKK6 pKa = 9.73HH7 pKa = 6.02CQNSLRR13 pKa = 11.84VGLNATRR20 pKa = 11.84QFSMLNKK27 pKa = 9.86PLITTSLSSSPISRR41 pKa = 11.84MPVQIPQPSTLSLLLNLIQRR61 pKa = 11.84RR62 pKa = 11.84FKK64 pKa = 11.22SRR66 pKa = 11.84GNTYY70 pKa = 10.24QPSTLKK76 pKa = 10.47RR77 pKa = 11.84KK78 pKa = 8.63RR79 pKa = 11.84TFGFLARR86 pKa = 11.84LRR88 pKa = 11.84SRR90 pKa = 11.84GGRR93 pKa = 11.84KK94 pKa = 8.67ILQRR98 pKa = 11.84RR99 pKa = 11.84KK100 pKa = 10.5AKK102 pKa = 9.78GRR104 pKa = 11.84WYY106 pKa = 9.08LTHH109 pKa = 7.23

Molecular weight: 12.57 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5132

0

5132

2713752

99

5748

528.8

59.42

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.813 ± 0.032

1.084 ± 0.011

6.012 ± 0.026

6.985 ± 0.035

4.43 ± 0.027

5.584 ± 0.036

2.23 ± 0.014

5.444 ± 0.025

6.807 ± 0.035

10.234 ± 0.041

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.987 ± 0.013

4.765 ± 0.022

4.87 ± 0.03

3.976 ± 0.025

4.621 ± 0.026

7.978 ± 0.033

5.539 ± 0.07

6.317 ± 0.028

1.097 ± 0.011

3.227 ± 0.017
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski