Nostoc minutum NIES-26

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Cyanobacteria/Melainabacteria group; Cyanobacteria; Nostocales; Nostocaceae; Nostoc; Nostoc minutum

Average proteome isoelectric point is 6.43

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 7588 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A367QJY6|A0A367QJY6_9NOSO HTH tetR-type domain-containing protein OS=Nostoc minutum NIES-26 OX=1844469 GN=A6770_28270 PE=4 SV=1
MM1 pKa = 7.45PVFVGTLFNDD11 pKa = 3.59TLTGYY16 pKa = 10.82VYY18 pKa = 11.16DD19 pKa = 4.47DD20 pKa = 4.17TLSGLAGNDD29 pKa = 2.92ILLGKK34 pKa = 10.0AGNDD38 pKa = 3.88DD39 pKa = 3.25ISGGEE44 pKa = 4.01GSDD47 pKa = 3.58WLDD50 pKa = 2.91GDD52 pKa = 4.67AGNDD56 pKa = 3.67TLRR59 pKa = 11.84GDD61 pKa = 3.74PFISEE66 pKa = 4.76DD67 pKa = 3.41EE68 pKa = 4.29DD69 pKa = 4.14EE70 pKa = 4.66ILRR73 pKa = 11.84LSPKK77 pKa = 10.47ARR79 pKa = 11.84DD80 pKa = 3.57TLRR83 pKa = 11.84GGLGNDD89 pKa = 3.74LLEE92 pKa = 4.97GGAGKK97 pKa = 8.63DD98 pKa = 3.48TLIGSAGDD106 pKa = 3.54DD107 pKa = 4.05VLWGGYY113 pKa = 8.8NDD115 pKa = 4.01YY116 pKa = 11.35EE117 pKa = 4.44GVLPFSSGDD126 pKa = 3.27YY127 pKa = 10.74GSFIEE132 pKa = 5.48KK133 pKa = 10.92YY134 pKa = 10.05NDD136 pKa = 3.26NGNDD140 pKa = 3.79LLVGGSGNDD149 pKa = 3.56TLHH152 pKa = 6.91GDD154 pKa = 4.35SIFADD159 pKa = 3.12ILFNGVFAIGGNDD172 pKa = 3.47TLDD175 pKa = 3.79GGSGNDD181 pKa = 3.81LLEE184 pKa = 4.67GSLGKK189 pKa = 10.35DD190 pKa = 3.23YY191 pKa = 11.2LYY193 pKa = 11.12GGSGNDD199 pKa = 3.37RR200 pKa = 11.84IYY202 pKa = 11.27GGTLNPNRR210 pKa = 11.84RR211 pKa = 11.84QSDD214 pKa = 3.25AGDD217 pKa = 3.3DD218 pKa = 3.68HH219 pKa = 8.48LYY221 pKa = 10.93GGSGDD226 pKa = 4.56DD227 pKa = 4.66LLAASIVDD235 pKa = 3.53IYY237 pKa = 10.9FGSGRR242 pKa = 11.84NDD244 pKa = 3.33FLDD247 pKa = 4.17GGAGNDD253 pKa = 3.65TLYY256 pKa = 11.46GEE258 pKa = 4.9AGSDD262 pKa = 3.76FLNGGAGNDD271 pKa = 3.66VLIGDD276 pKa = 3.78TDD278 pKa = 4.1FVFFRR283 pKa = 11.84QNTVDD288 pKa = 4.13VVDD291 pKa = 4.3TLTGGAGSDD300 pKa = 3.6QFILGSDD307 pKa = 3.46VHH309 pKa = 7.17GLTLYY314 pKa = 10.9GLAFGEE320 pKa = 4.16EE321 pKa = 4.56STFTEE326 pKa = 4.31YY327 pKa = 11.0AIITDD332 pKa = 4.63FNTNQDD338 pKa = 3.75IIQLAKK344 pKa = 10.45LYY346 pKa = 10.17YY347 pKa = 9.85PSDD350 pKa = 3.47LGLYY354 pKa = 9.46YY355 pKa = 9.9PANYY359 pKa = 9.97VLGSAPVGQTGTGIYY374 pKa = 9.35IDD376 pKa = 3.89QEE378 pKa = 4.01EE379 pKa = 4.25QADD382 pKa = 3.82VLVAVVQGVSNLSLSEE398 pKa = 4.17SYY400 pKa = 10.84FRR402 pKa = 11.84FVV404 pKa = 3.43

Molecular weight: 42.38 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A367S1R7|A0A367S1R7_9NOSO Acetyltransferase OS=Nostoc minutum NIES-26 OX=1844469 GN=A6770_35930 PE=4 SV=1
MM1 pKa = 7.31KK2 pKa = 10.03PNYY5 pKa = 9.07RR6 pKa = 11.84RR7 pKa = 11.84CISCRR12 pKa = 11.84KK13 pKa = 9.35VSSKK17 pKa = 10.83EE18 pKa = 3.79DD19 pKa = 3.04FWRR22 pKa = 11.84IVRR25 pKa = 11.84VFPSGKK31 pKa = 8.66VQLDD35 pKa = 3.07RR36 pKa = 11.84GMGRR40 pKa = 11.84SAYY43 pKa = 9.33ICPQPGCLQAAQKK56 pKa = 10.49KK57 pKa = 8.83NRR59 pKa = 11.84LGRR62 pKa = 11.84SLHH65 pKa = 6.06ASVPEE70 pKa = 4.0TLYY73 pKa = 9.09QTLWQRR79 pKa = 11.84LAA81 pKa = 3.68

Molecular weight: 9.37 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

7588

0

7588

2454932

31

8449

323.5

36.1

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.123 ± 0.03

0.996 ± 0.01

4.875 ± 0.024

6.275 ± 0.03

3.904 ± 0.018

6.49 ± 0.032

1.821 ± 0.014

6.735 ± 0.025

4.97 ± 0.028

10.965 ± 0.037

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.721 ± 0.013

4.5 ± 0.026

4.574 ± 0.023

5.558 ± 0.027

5.112 ± 0.023

6.508 ± 0.026

5.745 ± 0.027

6.62 ± 0.018

1.43 ± 0.012

3.075 ± 0.016
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski