Amino acid dipepetide frequency for Nostoc minutum NIES-26

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	7.465AlaAla: 7.465 ± 0.089	0.835AlaCys: 0.835 ± 0.019	4.026AlaAsp: 4.026 ± 0.048	5.146AlaGlu: 5.146 ± 0.064	2.961AlaPhe: 2.961 ± 0.036	5.339AlaGly: 5.339 ± 0.079	1.266AlaHis: 1.266 ± 0.025	6.912AlaIle: 6.912 ± 0.065	4.248AlaLys: 4.248 ± 0.047	8.603AlaLeu: 8.603 ± 0.085	1.556AlaMet: 1.556 ± 0.028	3.535AlaAsn: 3.535 ± 0.054	2.703AlaPro: 2.703 ± 0.03	4.187AlaGln: 4.187 ± 0.055	3.552AlaArg: 3.552 ± 0.043	4.988AlaSer: 4.988 ± 0.059	4.866AlaThr: 4.866 ± 0.052	5.713AlaVal: 5.713 ± 0.057	1.008AlaTrp: 1.008 ± 0.021	2.324AlaTyr: 2.324 ± 0.035	0.002AlaXaa: 0.002 ± 0.001
Cys	0.627CysAla: 0.627 ± 0.019	0.169CysCys: 0.169 ± 0.008	0.554CysAsp: 0.554 ± 0.018	0.544CysGlu: 0.544 ± 0.016	0.42CysPhe: 0.42 ± 0.014	0.748CysGly: 0.748 ± 0.019	0.288CysHis: 0.288 ± 0.01	0.615CysIle: 0.615 ± 0.016	0.382CysLys: 0.382 ± 0.012	1.111CysLeu: 1.111 ± 0.022	0.159CysMet: 0.159 ± 0.008	0.435CysAsn: 0.435 ± 0.015	0.493CysPro: 0.493 ± 0.015	0.625CysGln: 0.625 ± 0.019	0.543CysArg: 0.543 ± 0.013	0.629CysSer: 0.629 ± 0.019	0.475CysThr: 0.475 ± 0.014	0.594CysVal: 0.594 ± 0.016	0.158CysTrp: 0.158 ± 0.008	0.391CysTyr: 0.391 ± 0.014	0.0CysXaa: 0.0 ± 0.0
Asp	3.682AspAla: 3.682 ± 0.05	0.515AspCys: 0.515 ± 0.016	2.275AspAsp: 2.275 ± 0.047	3.198AspGlu: 3.198 ± 0.046	2.366AspPhe: 2.366 ± 0.035	3.353AspGly: 3.353 ± 0.059	0.528AspHis: 0.528 ± 0.015	3.534AspIle: 3.534 ± 0.045	2.445AspLys: 2.445 ± 0.039	5.432AspLeu: 5.432 ± 0.055	0.747AspMet: 0.747 ± 0.019	2.207AspAsn: 2.207 ± 0.04	2.208AspPro: 2.208 ± 0.031	1.361AspGln: 1.361 ± 0.023	3.407AspArg: 3.407 ± 0.039	3.022AspSer: 3.022 ± 0.044	2.583AspThr: 2.583 ± 0.045	3.051AspVal: 3.051 ± 0.041	0.954AspTrp: 0.954 ± 0.02	1.882AspTyr: 1.882 ± 0.032	0.0AspXaa: 0.0 ± 0.0
Glu	5.357GluAla: 5.357 ± 0.055	0.52GluCys: 0.52 ± 0.018	2.72GluAsp: 2.72 ± 0.038	4.073GluGlu: 4.073 ± 0.053	2.449GluPhe: 2.449 ± 0.035	3.083GluGly: 3.083 ± 0.041	1.03GluHis: 1.03 ± 0.022	4.959GluIle: 4.959 ± 0.051	3.506GluLys: 3.506 ± 0.044	7.135GluLeu: 7.135 ± 0.058	1.253GluMet: 1.253 ± 0.024	2.721GluAsn: 2.721 ± 0.035	2.356GluPro: 2.356 ± 0.034	3.941GluGln: 3.941 ± 0.045	3.532GluArg: 3.532 ± 0.046	3.504GluSer: 3.504 ± 0.044	3.434GluThr: 3.434 ± 0.049	4.411GluVal: 4.411 ± 0.054	0.779GluTrp: 0.779 ± 0.019	1.983GluTyr: 1.983 ± 0.033	0.0GluXaa: 0.0 ± 0.0
Phe	3.145PheAla: 3.145 ± 0.037	0.54PheCys: 0.54 ± 0.016	2.2PheAsp: 2.2 ± 0.033	2.227PheGlu: 2.227 ± 0.03	1.628PhePhe: 1.628 ± 0.028	2.837PheGly: 2.837 ± 0.041	0.734PheHis: 0.734 ± 0.021	2.43PheIle: 2.43 ± 0.037	1.625PheLys: 1.625 ± 0.031	4.116PheLeu: 4.116 ± 0.051	0.652PheMet: 0.652 ± 0.015	1.807PheAsn: 1.807 ± 0.03	1.696PhePro: 1.696 ± 0.026	1.86PheGln: 1.86 ± 0.028	1.763PheArg: 1.763 ± 0.03	2.989PheSer: 2.989 ± 0.04	2.291PheThr: 2.291 ± 0.03	2.447PheVal: 2.447 ± 0.036	0.694PheTrp: 0.694 ± 0.019	1.36PheTyr: 1.36 ± 0.026	0.001PheXaa: 0.001 ± 0.001
Gly	4.638GlyAla: 4.638 ± 0.071	0.78GlyCys: 0.78 ± 0.018	3.286GlyAsp: 3.286 ± 0.06	3.966GlyGlu: 3.966 ± 0.05	2.821GlyPhe: 2.821 ± 0.038	4.583GlyGly: 4.583 ± 0.088	1.267GlyHis: 1.267 ± 0.031	4.966GlyIle: 4.966 ± 0.052	4.027GlyLys: 4.027 ± 0.046	6.726GlyLeu: 6.726 ± 0.07	1.327GlyMet: 1.327 ± 0.025	3.153GlyAsn: 3.153 ± 0.082	1.239GlyPro: 1.239 ± 0.027	2.976GlyGln: 2.976 ± 0.042	3.222GlyArg: 3.222 ± 0.041	4.128GlySer: 4.128 ± 0.054	3.858GlyThr: 3.858 ± 0.07	4.557GlyVal: 4.557 ± 0.052	1.07GlyTrp: 1.07 ± 0.022	2.312GlyTyr: 2.312 ± 0.038	0.002GlyXaa: 0.002 ± 0.001
His	1.049HisAla: 1.049 ± 0.024	0.248HisCys: 0.248 ± 0.01	0.75HisAsp: 0.75 ± 0.02	0.972HisGlu: 0.972 ± 0.022	0.809HisPhe: 0.809 ± 0.022	1.1HisGly: 1.1 ± 0.023	0.544HisHis: 0.544 ± 0.018	1.138HisIle: 1.138 ± 0.02	0.841HisLys: 0.841 ± 0.02	2.219HisLeu: 2.219 ± 0.032	0.247HisMet: 0.247 ± 0.01	0.76HisAsn: 0.76 ± 0.017	1.271HisPro: 1.271 ± 0.025	1.165HisGln: 1.165 ± 0.025	1.068HisArg: 1.068 ± 0.022	1.207HisSer: 1.207 ± 0.024	0.944HisThr: 0.944 ± 0.025	0.848HisVal: 0.848 ± 0.019	0.364HisTrp: 0.364 ± 0.013	0.666HisTyr: 0.666 ± 0.016	0.0HisXaa: 0.0 ± 0.0
Ile	7.246IleAla: 7.246 ± 0.064	0.752IleCys: 0.752 ± 0.021	3.684IleAsp: 3.684 ± 0.036	4.311IleGlu: 4.311 ± 0.045	2.559IlePhe: 2.559 ± 0.038	4.422IleGly: 4.422 ± 0.049	1.274IleHis: 1.274 ± 0.024	3.734IleIle: 3.734 ± 0.045	3.262IleLys: 3.262 ± 0.041	6.663IleLeu: 6.663 ± 0.054	0.793IleMet: 0.793 ± 0.021	3.234IleAsn: 3.234 ± 0.052	3.443IlePro: 3.443 ± 0.047	3.27IleGln: 3.27 ± 0.037	3.135IleArg: 3.135 ± 0.037	4.508IleSer: 4.508 ± 0.047	3.751IleThr: 3.751 ± 0.052	4.403IleVal: 4.403 ± 0.047	0.9IleTrp: 0.9 ± 0.022	2.008IleTyr: 2.008 ± 0.031	0.001IleXaa: 0.001 ± 0.001
Lys	4.135LysAla: 4.135 ± 0.049	0.34LysCys: 0.34 ± 0.013	2.33LysAsp: 2.33 ± 0.036	2.891LysGlu: 2.891 ± 0.047	1.838LysPhe: 1.838 ± 0.026	2.747LysGly: 2.747 ± 0.043	0.916LysHis: 0.916 ± 0.021	3.62LysIle: 3.62 ± 0.047	2.392LysLys: 2.392 ± 0.04	5.704LysLeu: 5.704 ± 0.064	0.925LysMet: 0.925 ± 0.02	2.233LysAsn: 2.233 ± 0.037	2.491LysPro: 2.491 ± 0.035	3.014LysGln: 3.014 ± 0.042	2.41LysArg: 2.41 ± 0.035	3.196LysSer: 3.196 ± 0.041	3.078LysThr: 3.078 ± 0.047	3.325LysVal: 3.325 ± 0.04	0.527LysTrp: 0.527 ± 0.016	1.584LysTyr: 1.584 ± 0.03	0.001LysXaa: 0.001 ± 0.001
Leu	9.583LeuAla: 9.583 ± 0.073	1.048LeuCys: 1.048 ± 0.021	5.269LeuAsp: 5.269 ± 0.044	7.373LeuGlu: 7.373 ± 0.074	3.675LeuPhe: 3.675 ± 0.05	7.263LeuGly: 7.263 ± 0.074	1.968LeuHis: 1.968 ± 0.033	6.688LeuIle: 6.688 ± 0.057	5.749LeuLys: 5.749 ± 0.065	11.44LeuLeu: 11.44 ± 0.095	2.016LeuMet: 2.016 ± 0.03	4.685LeuAsn: 4.685 ± 0.053	5.61LeuPro: 5.61 ± 0.057	6.013LeuGln: 6.013 ± 0.06	5.635LeuArg: 5.635 ± 0.05	7.493LeuSer: 7.493 ± 0.063	6.551LeuThr: 6.551 ± 0.065	7.203LeuVal: 7.203 ± 0.054	1.522LeuTrp: 1.522 ± 0.034	2.866LeuTyr: 2.866 ± 0.04	0.004LeuXaa: 0.004 ± 0.002
Met	1.525MetAla: 1.525 ± 0.028	0.141MetCys: 0.141 ± 0.008	0.656MetAsp: 0.656 ± 0.017	0.931MetGlu: 0.931 ± 0.019	0.551MetPhe: 0.551 ± 0.015	1.205MetGly: 1.205 ± 0.027	0.316MetHis: 0.316 ± 0.01	0.965MetIle: 0.965 ± 0.019	0.911MetLys: 0.911 ± 0.021	1.836MetLeu: 1.836 ± 0.032	0.371MetMet: 0.371 ± 0.014	0.803MetAsn: 0.803 ± 0.016	0.924MetPro: 0.924 ± 0.023	0.95MetGln: 0.95 ± 0.023	0.919MetArg: 0.919 ± 0.021	1.304MetSer: 1.304 ± 0.021	1.248MetThr: 1.248 ± 0.022	1.161MetVal: 1.161 ± 0.023	0.15MetTrp: 0.15 ± 0.009	0.343MetTyr: 0.343 ± 0.014	0.0MetXaa: 0.0 ± 0.0
Asn	3.083AsnAla: 3.083 ± 0.048	0.471AsnCys: 0.471 ± 0.015	1.862AsnAsp: 1.862 ± 0.06	2.042AsnGlu: 2.042 ± 0.032	1.978AsnPhe: 1.978 ± 0.034	2.697AsnGly: 2.697 ± 0.048	0.853AsnHis: 0.853 ± 0.019	2.953AsnIle: 2.953 ± 0.05	1.954AsnLys: 1.954 ± 0.032	5.621AsnLeu: 5.621 ± 0.062	0.606AsnMet: 0.606 ± 0.016	2.272AsnAsn: 2.272 ± 0.045	2.915AsnPro: 2.915 ± 0.031	2.814AsnGln: 2.814 ± 0.043	2.352AsnArg: 2.352 ± 0.03	3.344AsnSer: 3.344 ± 0.044	2.471AsnThr: 2.471 ± 0.048	2.335AsnVal: 2.335 ± 0.035	0.758AsnTrp: 0.758 ± 0.018	1.622AsnTyr: 1.622 ± 0.03	0.001AsnXaa: 0.001 ± 0.001
Pro	3.16ProAla: 3.16 ± 0.045	0.338ProCys: 0.338 ± 0.012	2.866ProAsp: 2.866 ± 0.038	3.748ProGlu: 3.748 ± 0.048	1.666ProPhe: 1.666 ± 0.025	2.858ProGly: 2.858 ± 0.042	0.851ProHis: 0.851 ± 0.019	3.017ProIle: 3.017 ± 0.038	2.296ProLys: 2.296 ± 0.031	4.581ProLeu: 4.581 ± 0.052	0.683ProMet: 0.683 ± 0.017	2.277ProAsn: 2.277 ± 0.033	2.203ProPro: 2.203 ± 0.036	2.615ProGln: 2.615 ± 0.039	1.712ProArg: 1.712 ± 0.03	2.951ProSer: 2.951 ± 0.035	2.892ProThr: 2.892 ± 0.046	3.177ProVal: 3.177 ± 0.039	0.573ProTrp: 0.573 ± 0.017	1.277ProTyr: 1.277 ± 0.026	0.002ProXaa: 0.002 ± 0.001
Gln	4.934GlnAla: 4.934 ± 0.063	0.374GlnCys: 0.374 ± 0.014	2.196GlnAsp: 2.196 ± 0.032	3.742GlnGlu: 3.742 ± 0.044	1.797GlnPhe: 1.797 ± 0.026	3.261GlnGly: 3.261 ± 0.045	0.931GlnHis: 0.931 ± 0.021	3.816GlnIle: 3.816 ± 0.045	2.961GlnLys: 2.961 ± 0.037	6.348GlnLeu: 6.348 ± 0.067	1.073GlnMet: 1.073 ± 0.024	2.218GlnAsn: 2.218 ± 0.029	2.699GlnPro: 2.699 ± 0.05	4.155GlnGln: 4.155 ± 0.064	3.063GlnArg: 3.063 ± 0.035	3.128GlnSer: 3.128 ± 0.041	3.033GlnThr: 3.033 ± 0.04	3.833GlnVal: 3.833 ± 0.047	0.688GlnTrp: 0.688 ± 0.018	1.323GlnTyr: 1.323 ± 0.028	0.001GlnXaa: 0.001 ± 0.001
Arg	3.275ArgAla: 3.275 ± 0.039	0.53ArgCys: 0.53 ± 0.016	2.547ArgAsp: 2.547 ± 0.038	3.294ArgGlu: 3.294 ± 0.045	2.195ArgPhe: 2.195 ± 0.032	2.966ArgGly: 2.966 ± 0.037	1.039ArgHis: 1.039 ± 0.024	3.322ArgIle: 3.322 ± 0.038	2.328ArgLys: 2.328 ± 0.033	6.097ArgLeu: 6.097 ± 0.059	0.928ArgMet: 0.928 ± 0.021	2.121ArgAsn: 2.121 ± 0.031	2.015ArgPro: 2.015 ± 0.034	3.363ArgGln: 3.363 ± 0.038	3.009ArgArg: 3.009 ± 0.036	3.277ArgSer: 3.277 ± 0.04	2.634ArgThr: 2.634 ± 0.037	3.469ArgVal: 3.469 ± 0.043	0.812ArgTrp: 0.812 ± 0.017	1.894ArgTyr: 1.894 ± 0.031	0.001ArgXaa: 0.001 ± 0.001
Ser	4.67SerAla: 4.67 ± 0.047	0.618SerCys: 0.618 ± 0.018	3.286SerAsp: 3.286 ± 0.043	3.824SerGlu: 3.824 ± 0.042	2.619SerPhe: 2.619 ± 0.036	4.633SerGly: 4.633 ± 0.06	1.308SerHis: 1.308 ± 0.026	3.919SerIle: 3.919 ± 0.045	2.902SerLys: 2.902 ± 0.04	7.418SerLeu: 7.418 ± 0.069	1.093SerMet: 1.093 ± 0.022	3.009SerAsn: 3.009 ± 0.042	3.4SerPro: 3.4 ± 0.049	3.864SerGln: 3.864 ± 0.046	3.215SerArg: 3.215 ± 0.041	4.599SerSer: 4.599 ± 0.05	3.702SerThr: 3.702 ± 0.046	4.157SerVal: 4.157 ± 0.047	0.947SerTrp: 0.947 ± 0.021	1.895SerTyr: 1.895 ± 0.028	0.001SerXaa: 0.001 ± 0.001
Thr	4.86ThrAla: 4.86 ± 0.063	0.49ThrCys: 0.49 ± 0.014	2.756ThrAsp: 2.756 ± 0.037	3.319ThrGlu: 3.319 ± 0.039	2.185ThrPhe: 2.185 ± 0.03	4.3ThrGly: 4.3 ± 0.062	1.044ThrHis: 1.044 ± 0.021	3.872ThrIle: 3.872 ± 0.055	2.51ThrLys: 2.51 ± 0.037	6.294ThrLeu: 6.294 ± 0.058	0.752ThrMet: 0.752 ± 0.016	2.504ThrAsn: 2.504 ± 0.037	3.358ThrPro: 3.358 ± 0.049	3.08ThrGln: 3.08 ± 0.038	2.435ThrArg: 2.435 ± 0.034	3.669ThrSer: 3.669 ± 0.047	3.578ThrThr: 3.578 ± 0.046	4.075ThrVal: 4.075 ± 0.049	0.747ThrTrp: 0.747 ± 0.019	1.619ThrTyr: 1.619 ± 0.027	0.0ThrXaa: 0.0 ± 0.0
Val	5.869ValAla: 5.869 ± 0.057	0.691ValCys: 0.691 ± 0.016	3.371ValAsp: 3.371 ± 0.039	4.464ValGlu: 4.464 ± 0.045	2.541ValPhe: 2.541 ± 0.036	4.502ValGly: 4.502 ± 0.052	1.014ValHis: 1.014 ± 0.019	4.276ValIle: 4.276 ± 0.043	3.388ValLys: 3.388 ± 0.042	6.804ValLeu: 6.804 ± 0.065	1.315ValMet: 1.315 ± 0.025	3.052ValAsn: 3.052 ± 0.045	2.839ValPro: 2.839 ± 0.042	3.061ValGln: 3.061 ± 0.035	3.306ValArg: 3.306 ± 0.043	4.286ValSer: 4.286 ± 0.047	3.876ValThr: 3.876 ± 0.048	4.823ValVal: 4.823 ± 0.056	0.859ValTrp: 0.859 ± 0.022	1.858ValTyr: 1.858 ± 0.027	0.001ValXaa: 0.001 ± 0.001
Trp	0.869TrpAla: 0.869 ± 0.019	0.162TrpCys: 0.162 ± 0.008	0.664TrpAsp: 0.664 ± 0.023	0.898TrpGlu: 0.898 ± 0.022	0.602TrpPhe: 0.602 ± 0.018	0.954TrpGly: 0.954 ± 0.022	0.37TrpHis: 0.37 ± 0.013	0.851TrpIle: 0.851 ± 0.022	0.637TrpLys: 0.637 ± 0.019	1.867TrpLeu: 1.867 ± 0.032	0.287TrpMet: 0.287 ± 0.012	0.699TrpAsn: 0.699 ± 0.023	0.269TrpPro: 0.269 ± 0.011	1.243TrpGln: 1.243 ± 0.026	0.854TrpArg: 0.854 ± 0.019	0.848TrpSer: 0.848 ± 0.02	0.596TrpThr: 0.596 ± 0.018	0.962TrpVal: 0.962 ± 0.019	0.237TrpTrp: 0.237 ± 0.01	0.437TrpTyr: 0.437 ± 0.013	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.059TyrAla: 2.059 ± 0.03	0.401TyrCys: 0.401 ± 0.014	1.451TyrAsp: 1.451 ± 0.028	1.784TyrGlu: 1.784 ± 0.031	1.382TyrPhe: 1.382 ± 0.029	2.05TyrGly: 2.05 ± 0.035	0.667TyrHis: 0.667 ± 0.015	1.765TyrIle: 1.765 ± 0.03	1.333TyrLys: 1.333 ± 0.026	3.634TyrLeu: 3.634 ± 0.041	0.425TyrMet: 0.425 ± 0.014	1.277TyrAsn: 1.277 ± 0.03	1.609TyrPro: 1.609 ± 0.024	2.018TyrGln: 2.018 ± 0.03	1.985TyrArg: 1.985 ± 0.032	2.008TyrSer: 2.008 ± 0.029	1.586TyrThr: 1.586 ± 0.028	1.655TyrVal: 1.655 ± 0.025	0.557TyrTrp: 0.557 ± 0.016	1.108TyrTyr: 1.108 ± 0.026	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.002XaaAla: 0.002 ± 0.001	0.0XaaCys: 0.0 ± 0.0	0.001XaaAsp: 0.001 ± 0.001	0.001XaaGlu: 0.001 ± 0.001	0.001XaaPhe: 0.001 ± 0.001	0.002XaaGly: 0.002 ± 0.001	0.0XaaHis: 0.0 ± 0.0	0.001XaaIle: 0.001 ± 0.001	0.0XaaLys: 0.0 ± 0.0	0.002XaaLeu: 0.002 ± 0.001	0.0XaaMet: 0.0 ± 0.0	0.002XaaAsn: 0.002 ± 0.001	0.0XaaPro: 0.0 ± 0.0	0.002XaaGln: 0.002 ± 0.001	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.001XaaThr: 0.001 ± 0.001	0.001XaaVal: 0.001 ± 0.001	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.001XaaXaa: 0.001 ± 0.001

Statistics based on 7588 proteins (2454932 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

---