Bacillus coahuilensis p1.1.43

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus; Bacillus coahuilensis

Average proteome isoelectric point is 6.13

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3184 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A147KAK5|A0A147KAK5_9BACI Transporter OS=Bacillus coahuilensis p1.1.43 OX=1150625 GN=Q75_04465 PE=4 SV=1
MM1 pKa = 7.46PAFVGVVQVISVGSSGIVNIGDD23 pKa = 3.67VFQMSPYY30 pKa = 8.88STAKK34 pKa = 8.68TFSGAGSFNTGDD46 pKa = 3.3GLKK49 pKa = 9.92IYY51 pKa = 10.7NEE53 pKa = 4.06YY54 pKa = 10.85SATNVQDD61 pKa = 3.63PDD63 pKa = 5.21GIDD66 pKa = 3.12SGVILNAA73 pKa = 4.14

Molecular weight: 7.48 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A147KCH3|A0A147KCH3_9BACI Uncharacterized protein OS=Bacillus coahuilensis p1.1.43 OX=1150625 GN=Q75_00205 PE=4 SV=1
MM1 pKa = 7.44KK2 pKa = 9.6RR3 pKa = 11.84TFQPNNRR10 pKa = 11.84KK11 pKa = 9.27RR12 pKa = 11.84SKK14 pKa = 9.57VHH16 pKa = 5.77GFRR19 pKa = 11.84ARR21 pKa = 11.84MSSANGRR28 pKa = 11.84KK29 pKa = 8.98VLASRR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84KK37 pKa = 8.73GRR39 pKa = 11.84KK40 pKa = 8.76VLSAA44 pKa = 4.05

Molecular weight: 5.13 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3184

0

3184

901207

26

2236

283.0

31.88

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.272 ± 0.046

0.659 ± 0.012

5.036 ± 0.035

8.029 ± 0.052

4.549 ± 0.038

6.793 ± 0.043

2.195 ± 0.021

7.722 ± 0.041

6.588 ± 0.043

10.018 ± 0.054

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.716 ± 0.022

4.186 ± 0.036

3.52 ± 0.024

3.73 ± 0.03

4.114 ± 0.035

6.439 ± 0.039

5.543 ± 0.029

7.239 ± 0.036

1.012 ± 0.017

3.642 ± 0.03
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski