Klebsiella phage ST405-OXA48phi1.2

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; unclassified Myoviridae

Average proteome isoelectric point is 6.27

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 66 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A482IJB1|A0A482IJB1_9CAUD SGNH_hydro domain-containing protein OS=Klebsiella phage ST405-OXA48phi1.2 OX=2510484 PE=4 SV=1
MM1 pKa = 7.39AVSEE5 pKa = 4.68IPLSPEE11 pKa = 3.45NQRR14 pKa = 11.84FSISVAGQSLQMAVTWRR31 pKa = 11.84GAFWCLDD38 pKa = 3.14IMDD41 pKa = 4.47STGADD46 pKa = 4.01LIKK49 pKa = 10.53GIPLITGANLLAQYY63 pKa = 10.19RR64 pKa = 11.84YY65 pKa = 10.29LGLGFSLYY73 pKa = 10.85VNCDD77 pKa = 3.1DD78 pKa = 5.26PANDD82 pKa = 4.58NPTQTDD88 pKa = 3.68LGIKK92 pKa = 8.43SHH94 pKa = 6.76LYY96 pKa = 10.57AVTEE100 pKa = 4.11

Molecular weight: 10.88 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A482IKU0|A0A482IKU0_9CAUD Uncharacterized protein OS=Klebsiella phage ST405-OXA48phi1.2 OX=2510484 PE=4 SV=1
MM1 pKa = 7.59KK2 pKa = 10.22IEE4 pKa = 4.37FNDD7 pKa = 3.41KK8 pKa = 10.84GVIATATITSTVFEE22 pKa = 4.3FRR24 pKa = 11.84LHH26 pKa = 5.61NRR28 pKa = 11.84AVDD31 pKa = 3.52TALFLAPSVRR41 pKa = 11.84AKK43 pKa = 10.53RR44 pKa = 11.84SGFFVLKK51 pKa = 9.58TVITGKK57 pKa = 8.86TSHH60 pKa = 6.24VLRR63 pKa = 11.84AYY65 pKa = 9.76KK66 pKa = 10.15AIKK69 pKa = 10.51AEE71 pKa = 4.08ASRR74 pKa = 4.01

Molecular weight: 8.2 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

66

0

66

12221

35

639

185.2

20.4

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.77 ± 0.355

1.096 ± 0.145

6.08 ± 0.192

5.769 ± 0.313

3.453 ± 0.225

7.831 ± 0.29

1.555 ± 0.178

5.965 ± 0.206

5.073 ± 0.258

8.019 ± 0.229

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.488 ± 0.147

4.697 ± 0.205

4.091 ± 0.18

3.87 ± 0.146

5.671 ± 0.358

6.947 ± 0.293

6.055 ± 0.366

6.767 ± 0.327

1.849 ± 0.164

2.954 ± 0.248
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski