Sporosarcina globispora (Bacillus globisporus)

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Planococcaceae; Sporosarcina

Average proteome isoelectric point is 6.31

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5125 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0M0GAU3|A0A0M0GAU3_SPOGL Polysaccharide polymerase OS=Sporosarcina globispora OX=1459 GN=AF332_06700 PE=4 SV=1
MM1 pKa = 7.27GVIDD5 pKa = 4.03TGVDD9 pKa = 3.54YY10 pKa = 11.19NHH12 pKa = 7.82PDD14 pKa = 2.96LKK16 pKa = 10.78AAYY19 pKa = 8.93KK20 pKa = 10.49GGYY23 pKa = 9.98DD24 pKa = 5.47FIDD27 pKa = 3.96NDD29 pKa = 4.68DD30 pKa = 4.78DD31 pKa = 4.7PMEE34 pKa = 4.25TTYY37 pKa = 11.68DD38 pKa = 3.35DD39 pKa = 3.17WKK41 pKa = 10.77AASGYY46 pKa = 9.91PEE48 pKa = 4.25TNQGSTYY55 pKa = 7.8YY56 pKa = 9.42TEE58 pKa = 5.29HH59 pKa = 6.21GTHH62 pKa = 5.58VSGNIVGRR70 pKa = 11.84AANDD74 pKa = 2.9SDD76 pKa = 4.11YY77 pKa = 11.44KK78 pKa = 10.96VIGVAPEE85 pKa = 3.91ADD87 pKa = 3.34LYY89 pKa = 11.22AYY91 pKa = 9.86RR92 pKa = 11.84VLGKK96 pKa = 10.27YY97 pKa = 10.63GSGSNSAAIAGIDD110 pKa = 3.5RR111 pKa = 11.84AVADD115 pKa = 4.09GMDD118 pKa = 4.33VINLSLGAQTNNPLDD133 pKa = 4.11ASSLAVDD140 pKa = 3.57NAVLSGVAAVVAAGNTGDD158 pKa = 5.04LGNSTLGSPGEE169 pKa = 4.04AAA171 pKa = 4.5

Molecular weight: 17.52 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0M0G921|A0A0M0G921_SPOGL Sigma70_r2 domain-containing protein OS=Sporosarcina globispora OX=1459 GN=AF332_03720 PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 9.42RR3 pKa = 11.84TYY5 pKa = 10.34QPNKK9 pKa = 8.21RR10 pKa = 11.84KK11 pKa = 9.62RR12 pKa = 11.84SKK14 pKa = 9.59VHH16 pKa = 5.83GFRR19 pKa = 11.84SRR21 pKa = 11.84MSTPNGRR28 pKa = 11.84NVLARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84KK37 pKa = 9.05GRR39 pKa = 11.84KK40 pKa = 8.7VLSAA44 pKa = 4.05

Molecular weight: 5.27 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5125

0

5125

1462172

26

1940

285.3

31.98

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.295 ± 0.041

0.719 ± 0.012

5.111 ± 0.027

7.657 ± 0.043

4.606 ± 0.032

7.073 ± 0.03

2.013 ± 0.018

7.834 ± 0.03

7.19 ± 0.03

9.627 ± 0.042

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.814 ± 0.02

4.427 ± 0.027

3.688 ± 0.019

3.516 ± 0.022

3.976 ± 0.027

6.188 ± 0.026

5.133 ± 0.027

6.593 ± 0.032

1.033 ± 0.012

3.506 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski