Amino acid dipepetide frequency for Sporosarcina globispora (Bacillus globisporus)

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	7.089AlaAla: 7.089 ± 0.106	0.573AlaCys: 0.573 ± 0.022	3.904AlaAsp: 3.904 ± 0.057	5.475AlaGlu: 5.475 ± 0.06	3.32AlaPhe: 3.32 ± 0.053	6.131AlaGly: 6.131 ± 0.078	1.275AlaHis: 1.275 ± 0.029	5.74AlaIle: 5.74 ± 0.076	4.789AlaLys: 4.789 ± 0.077	7.146AlaLeu: 7.146 ± 0.078	2.059AlaMet: 2.059 ± 0.041	2.76AlaAsn: 2.76 ± 0.045	2.143AlaPro: 2.143 ± 0.046	2.112AlaGln: 2.112 ± 0.034	2.621AlaArg: 2.621 ± 0.041	4.198AlaSer: 4.198 ± 0.055	2.868AlaThr: 2.868 ± 0.053	5.818AlaVal: 5.818 ± 0.071	0.607AlaTrp: 0.607 ± 0.021	2.322AlaTyr: 2.322 ± 0.04	0.001AlaXaa: 0.001 ± 0.001
Cys	0.427CysAla: 0.427 ± 0.018	0.109CysCys: 0.109 ± 0.01	0.375CysAsp: 0.375 ± 0.017	0.486CysGlu: 0.486 ± 0.018	0.303CysPhe: 0.303 ± 0.013	0.668CysGly: 0.668 ± 0.024	0.189CysHis: 0.189 ± 0.012	0.533CysIle: 0.533 ± 0.021	0.384CysLys: 0.384 ± 0.019	0.646CysLeu: 0.646 ± 0.022	0.206CysMet: 0.206 ± 0.013	0.27CysAsn: 0.27 ± 0.013	0.358CysPro: 0.358 ± 0.02	0.243CysGln: 0.243 ± 0.013	0.347CysArg: 0.347 ± 0.016	0.585CysSer: 0.585 ± 0.021	0.378CysThr: 0.378 ± 0.014	0.378CysVal: 0.378 ± 0.017	0.064CysTrp: 0.064 ± 0.007	0.243CysTyr: 0.243 ± 0.013	0.001CysXaa: 0.001 ± 0.001
Asp	3.293AspAla: 3.293 ± 0.057	0.361AspCys: 0.361 ± 0.017	2.352AspAsp: 2.352 ± 0.057	4.342AspGlu: 4.342 ± 0.067	2.565AspPhe: 2.565 ± 0.036	3.48AspGly: 3.48 ± 0.057	1.139AspHis: 1.139 ± 0.027	4.188AspIle: 4.188 ± 0.062	3.273AspLys: 3.273 ± 0.048	5.198AspLeu: 5.198 ± 0.05	1.392AspMet: 1.392 ± 0.03	1.84AspAsn: 1.84 ± 0.043	2.161AspPro: 2.161 ± 0.039	1.925AspGln: 1.925 ± 0.041	2.303AspArg: 2.303 ± 0.038	2.819AspSer: 2.819 ± 0.053	2.217AspThr: 2.217 ± 0.045	3.478AspVal: 3.478 ± 0.052	0.661AspTrp: 0.661 ± 0.021	2.12AspTyr: 2.12 ± 0.045	0.0AspXaa: 0.0 ± 0.0
Glu	5.529GluAla: 5.529 ± 0.071	0.425GluCys: 0.425 ± 0.018	3.935GluAsp: 3.935 ± 0.056	7.344GluGlu: 7.344 ± 0.098	2.787GluPhe: 2.787 ± 0.051	4.599GluGly: 4.599 ± 0.06	1.47GluHis: 1.47 ± 0.03	5.84GluIle: 5.84 ± 0.071	6.996GluLys: 6.996 ± 0.082	7.166GluLeu: 7.166 ± 0.08	2.394GluMet: 2.394 ± 0.039	3.914GluAsn: 3.914 ± 0.056	1.997GluPro: 1.997 ± 0.039	2.972GluGln: 2.972 ± 0.047	3.35GluArg: 3.35 ± 0.057	3.841GluSer: 3.841 ± 0.053	3.853GluThr: 3.853 ± 0.053	4.828GluVal: 4.828 ± 0.058	0.87GluTrp: 0.87 ± 0.027	2.458GluTyr: 2.458 ± 0.043	0.001GluXaa: 0.001 ± 0.001
Phe	3.216PheAla: 3.216 ± 0.052	0.354PheCys: 0.354 ± 0.017	2.253PheAsp: 2.253 ± 0.043	2.938PheGlu: 2.938 ± 0.048	2.336PhePhe: 2.336 ± 0.051	3.343PheGly: 3.343 ± 0.059	0.96PheHis: 0.96 ± 0.032	3.937PheIle: 3.937 ± 0.069	2.675PheLys: 2.675 ± 0.048	4.691PheLeu: 4.691 ± 0.076	1.217PheMet: 1.217 ± 0.03	2.045PheAsn: 2.045 ± 0.04	1.754PhePro: 1.754 ± 0.037	1.581PheGln: 1.581 ± 0.03	1.605PheArg: 1.605 ± 0.031	3.344PheSer: 3.344 ± 0.052	2.574PheThr: 2.574 ± 0.048	2.903PheVal: 2.903 ± 0.046	0.525PheTrp: 0.525 ± 0.019	1.803PheTyr: 1.803 ± 0.037	0.0PheXaa: 0.0 ± 0.0
Gly	5.02GlyAla: 5.02 ± 0.075	0.639GlyCys: 0.639 ± 0.023	3.29GlyAsp: 3.29 ± 0.057	4.642GlyGlu: 4.642 ± 0.067	3.54GlyPhe: 3.54 ± 0.048	4.839GlyGly: 4.839 ± 0.071	1.383GlyHis: 1.383 ± 0.035	6.3GlyIle: 6.3 ± 0.083	5.357GlyLys: 5.357 ± 0.063	6.698GlyLeu: 6.698 ± 0.076	2.193GlyMet: 2.193 ± 0.038	2.933GlyAsn: 2.933 ± 0.056	1.867GlyPro: 1.867 ± 0.04	2.307GlyGln: 2.307 ± 0.046	2.786GlyArg: 2.786 ± 0.055	4.273GlySer: 4.273 ± 0.052	4.162GlyThr: 4.162 ± 0.061	4.843GlyVal: 4.843 ± 0.064	0.805GlyTrp: 0.805 ± 0.027	2.853GlyTyr: 2.853 ± 0.048	0.001GlyXaa: 0.001 ± 0.001
His	1.229HisAla: 1.229 ± 0.032	0.22HisCys: 0.22 ± 0.014	1.023HisAsp: 1.023 ± 0.028	1.352HisGlu: 1.352 ± 0.03	1.091HisPhe: 1.091 ± 0.03	1.395HisGly: 1.395 ± 0.032	0.601HisHis: 0.601 ± 0.021	1.464HisIle: 1.464 ± 0.033	1.114HisLys: 1.114 ± 0.031	2.022HisLeu: 2.022 ± 0.038	0.52HisMet: 0.52 ± 0.021	0.786HisAsn: 0.786 ± 0.024	1.133HisPro: 1.133 ± 0.025	0.75HisGln: 0.75 ± 0.025	0.858HisArg: 0.858 ± 0.024	1.252HisSer: 1.252 ± 0.03	0.992HisThr: 0.992 ± 0.028	1.28HisVal: 1.28 ± 0.03	0.205HisTrp: 0.205 ± 0.012	0.842HisTyr: 0.842 ± 0.022	0.001HisXaa: 0.001 ± 0.001
Ile	5.981IleAla: 5.981 ± 0.068	0.634IleCys: 0.634 ± 0.019	4.14IleAsp: 4.14 ± 0.058	5.655IleGlu: 5.655 ± 0.066	3.437IlePhe: 3.437 ± 0.061	6.029IleGly: 6.029 ± 0.066	1.673IleHis: 1.673 ± 0.035	5.951IleIle: 5.951 ± 0.073	5.069IleLys: 5.069 ± 0.061	7.296IleLeu: 7.296 ± 0.083	1.913IleMet: 1.913 ± 0.038	3.409IleAsn: 3.409 ± 0.052	3.4IlePro: 3.4 ± 0.048	2.786IleGln: 2.786 ± 0.042	3.172IleArg: 3.172 ± 0.054	5.372IleSer: 5.372 ± 0.066	4.285IleThr: 4.285 ± 0.062	4.984IleVal: 4.984 ± 0.064	0.703IleTrp: 0.703 ± 0.024	2.448IleTyr: 2.448 ± 0.044	0.0IleXaa: 0.0 ± 0.0
Lys	5.008LysAla: 5.008 ± 0.065	0.377LysCys: 0.377 ± 0.017	4.061LysAsp: 4.061 ± 0.051	7.245LysGlu: 7.245 ± 0.082	2.143LysPhe: 2.143 ± 0.039	4.808LysGly: 4.808 ± 0.059	1.364LysHis: 1.364 ± 0.033	4.964LysIle: 4.964 ± 0.058	6.222LysLys: 6.222 ± 0.079	6.02LysLeu: 6.02 ± 0.079	2.333LysMet: 2.333 ± 0.043	3.599LysAsn: 3.599 ± 0.05	2.413LysPro: 2.413 ± 0.041	2.844LysGln: 2.844 ± 0.049	3.133LysArg: 3.133 ± 0.048	3.795LysSer: 3.795 ± 0.054	3.703LysThr: 3.703 ± 0.048	4.699LysVal: 4.699 ± 0.061	0.851LysTrp: 0.851 ± 0.024	2.319LysTyr: 2.319 ± 0.043	0.0LysXaa: 0.0 ± 0.0
Leu	7.372LeuAla: 7.372 ± 0.074	0.66LeuCys: 0.66 ± 0.024	4.875LeuAsp: 4.875 ± 0.06	6.737LeuGlu: 6.737 ± 0.078	4.715LeuPhe: 4.715 ± 0.087	6.377LeuGly: 6.377 ± 0.068	1.84LeuHis: 1.84 ± 0.032	7.297LeuIle: 7.297 ± 0.088	7.092LeuLys: 7.092 ± 0.07	9.802LeuLeu: 9.802 ± 0.118	2.537LeuMet: 2.537 ± 0.042	4.31LeuAsn: 4.31 ± 0.056	3.882LeuPro: 3.882 ± 0.053	3.428LeuGln: 3.428 ± 0.044	3.488LeuArg: 3.488 ± 0.056	6.805LeuSer: 6.805 ± 0.073	5.519LeuThr: 5.519 ± 0.07	5.651LeuVal: 5.651 ± 0.068	0.81LeuTrp: 0.81 ± 0.024	3.074LeuTyr: 3.074 ± 0.05	0.001LeuXaa: 0.001 ± 0.001
Met	2.284MetAla: 2.284 ± 0.044	0.131MetCys: 0.131 ± 0.009	1.517MetAsp: 1.517 ± 0.03	2.151MetGlu: 2.151 ± 0.04	1.087MetPhe: 1.087 ± 0.028	1.892MetGly: 1.892 ± 0.037	0.447MetHis: 0.447 ± 0.02	2.18MetIle: 2.18 ± 0.037	2.568MetLys: 2.568 ± 0.037	2.554MetLeu: 2.554 ± 0.048	0.825MetMet: 0.825 ± 0.026	1.587MetAsn: 1.587 ± 0.034	1.097MetPro: 1.097 ± 0.05	0.894MetGln: 0.894 ± 0.027	1.078MetArg: 1.078 ± 0.025	1.674MetSer: 1.674 ± 0.033	1.566MetThr: 1.566 ± 0.033	1.671MetVal: 1.671 ± 0.036	0.198MetTrp: 0.198 ± 0.012	0.739MetTyr: 0.739 ± 0.022	0.001MetXaa: 0.001 ± 0.001
Asn	2.887AsnAla: 2.887 ± 0.048	0.304AsnCys: 0.304 ± 0.014	2.2AsnAsp: 2.2 ± 0.037	3.585AsnGlu: 3.585 ± 0.05	1.694AsnPhe: 1.694 ± 0.041	3.443AsnGly: 3.443 ± 0.057	0.981AsnHis: 0.981 ± 0.024	3.414AsnIle: 3.414 ± 0.051	3.05AsnLys: 3.05 ± 0.051	3.843AsnLeu: 3.843 ± 0.053	1.23AsnMet: 1.23 ± 0.03	2.026AsnAsn: 2.026 ± 0.041	2.253AsnPro: 2.253 ± 0.038	1.769AsnGln: 1.769 ± 0.036	1.907AsnArg: 1.907 ± 0.036	2.575AsnSer: 2.575 ± 0.044	2.21AsnThr: 2.21 ± 0.036	2.845AsnVal: 2.845 ± 0.041	0.538AsnTrp: 0.538 ± 0.021	1.518AsnTyr: 1.518 ± 0.039	0.001AsnXaa: 0.001 ± 0.001
Pro	2.752ProAla: 2.752 ± 0.051	0.206ProCys: 0.206 ± 0.012	2.186ProAsp: 2.186 ± 0.04	3.282ProGlu: 3.282 ± 0.045	2.087ProPhe: 2.087 ± 0.038	2.604ProGly: 2.604 ± 0.052	0.832ProHis: 0.832 ± 0.025	2.738ProIle: 2.738 ± 0.038	2.212ProLys: 2.212 ± 0.04	3.465ProLeu: 3.465 ± 0.048	0.844ProMet: 0.844 ± 0.025	1.625ProAsn: 1.625 ± 0.034	1.069ProPro: 1.069 ± 0.025	1.13ProGln: 1.13 ± 0.039	1.114ProArg: 1.114 ± 0.028	2.398ProSer: 2.398 ± 0.037	1.625ProThr: 1.625 ± 0.037	2.898ProVal: 2.898 ± 0.047	0.362ProTrp: 0.362 ± 0.015	1.45ProTyr: 1.45 ± 0.031	0.0ProXaa: 0.0 ± 0.0
Gln	2.653GlnAla: 2.653 ± 0.046	0.21GlnCys: 0.21 ± 0.013	1.641GlnAsp: 1.641 ± 0.035	2.781GlnGlu: 2.781 ± 0.042	1.581GlnPhe: 1.581 ± 0.037	2.106GlnGly: 2.106 ± 0.038	0.676GlnHis: 0.676 ± 0.021	2.512GlnIle: 2.512 ± 0.045	2.714GlnLys: 2.714 ± 0.044	3.588GlnLeu: 3.588 ± 0.046	1.107GlnMet: 1.107 ± 0.036	1.641GlnAsn: 1.641 ± 0.034	1.215GlnPro: 1.215 ± 0.033	1.483GlnGln: 1.483 ± 0.038	1.425GlnArg: 1.425 ± 0.032	2.092GlnSer: 2.092 ± 0.039	1.922GlnThr: 1.922 ± 0.04	2.113GlnVal: 2.113 ± 0.034	0.342GlnTrp: 0.342 ± 0.015	1.356GlnTyr: 1.356 ± 0.032	0.001GlnXaa: 0.001 ± 0.001
Arg	2.373ArgAla: 2.373 ± 0.043	0.268ArgCys: 0.268 ± 0.013	2.044ArgAsp: 2.044 ± 0.038	3.281ArgGlu: 3.281 ± 0.058	1.894ArgPhe: 1.894 ± 0.039	2.4ArgGly: 2.4 ± 0.04	0.782ArgHis: 0.782 ± 0.024	3.119ArgIle: 3.119 ± 0.051	3.37ArgLys: 3.37 ± 0.048	3.805ArgLeu: 3.805 ± 0.059	1.253ArgMet: 1.253 ± 0.033	1.929ArgAsn: 1.929 ± 0.037	1.294ArgPro: 1.294 ± 0.032	1.487ArgGln: 1.487 ± 0.034	1.804ArgArg: 1.804 ± 0.037	2.247ArgSer: 2.247 ± 0.041	2.028ArgThr: 2.028 ± 0.037	2.493ArgVal: 2.493 ± 0.05	0.42ArgTrp: 0.42 ± 0.016	1.464ArgTyr: 1.464 ± 0.033	0.001ArgXaa: 0.001 ± 0.001
Ser	4.322SerAla: 4.322 ± 0.067	0.457SerCys: 0.457 ± 0.017	2.921SerAsp: 2.921 ± 0.05	4.226SerGlu: 4.226 ± 0.053	3.385SerPhe: 3.385 ± 0.058	4.843SerGly: 4.843 ± 0.058	1.222SerHis: 1.222 ± 0.026	5.114SerIle: 5.114 ± 0.066	3.967SerLys: 3.967 ± 0.06	6.317SerLeu: 6.317 ± 0.073	1.762SerMet: 1.762 ± 0.03	2.409SerAsn: 2.409 ± 0.048	2.288SerPro: 2.288 ± 0.041	2.038SerGln: 2.038 ± 0.038	2.469SerArg: 2.469 ± 0.043	4.203SerSer: 4.203 ± 0.068	2.894SerThr: 2.894 ± 0.047	4.059SerVal: 4.059 ± 0.057	0.709SerTrp: 0.709 ± 0.023	2.28SerTyr: 2.28 ± 0.044	0.0SerXaa: 0.0 ± 0.0
Thr	4.244ThrAla: 4.244 ± 0.062	0.332ThrCys: 0.332 ± 0.014	2.701ThrAsp: 2.701 ± 0.045	3.431ThrGlu: 3.431 ± 0.053	2.43ThrPhe: 2.43 ± 0.049	4.46ThrGly: 4.46 ± 0.057	0.962ThrHis: 0.962 ± 0.027	4.196ThrIle: 4.196 ± 0.049	3.121ThrLys: 3.121 ± 0.05	4.925ThrLeu: 4.925 ± 0.064	1.271ThrMet: 1.271 ± 0.029	2.171ThrAsn: 2.171 ± 0.032	2.147ThrPro: 2.147 ± 0.041	1.317ThrGln: 1.317 ± 0.032	1.742ThrArg: 1.742 ± 0.036	3.095ThrSer: 3.095 ± 0.052	2.405ThrThr: 2.405 ± 0.05	4.101ThrVal: 4.101 ± 0.062	0.488ThrTrp: 0.488 ± 0.016	1.795ThrTyr: 1.795 ± 0.032	0.0ThrXaa: 0.0 ± 0.0
Val	4.437ValAla: 4.437 ± 0.07	0.552ValCys: 0.552 ± 0.022	3.24ValAsp: 3.24 ± 0.049	4.357ValGlu: 4.357 ± 0.057	3.234ValPhe: 3.234 ± 0.055	4.114ValGly: 4.114 ± 0.062	1.293ValHis: 1.293 ± 0.03	5.475ValIle: 5.475 ± 0.063	4.878ValLys: 4.878 ± 0.062	6.578ValLeu: 6.578 ± 0.078	1.83ValMet: 1.83 ± 0.034	3.038ValAsn: 3.038 ± 0.048	2.696ValPro: 2.696 ± 0.046	2.314ValGln: 2.314 ± 0.042	2.537ValArg: 2.537 ± 0.042	4.46ValSer: 4.46 ± 0.054	3.814ValThr: 3.814 ± 0.054	4.26ValVal: 4.26 ± 0.068	0.627ValTrp: 0.627 ± 0.019	2.199ValTyr: 2.199 ± 0.04	0.0ValXaa: 0.0 ± 0.0
Trp	0.673TrpAla: 0.673 ± 0.023	0.072TrpCys: 0.072 ± 0.007	0.551TrpAsp: 0.551 ± 0.021	0.693TrpGlu: 0.693 ± 0.023	0.547TrpPhe: 0.547 ± 0.021	0.714TrpGly: 0.714 ± 0.022	0.222TrpHis: 0.222 ± 0.011	0.847TrpIle: 0.847 ± 0.026	0.795TrpLys: 0.795 ± 0.023	1.081TrpLeu: 1.081 ± 0.031	0.357TrpMet: 0.357 ± 0.015	0.55TrpAsn: 0.55 ± 0.021	0.282TrpPro: 0.282 ± 0.014	0.354TrpGln: 0.354 ± 0.017	0.381TrpArg: 0.381 ± 0.018	0.582TrpSer: 0.582 ± 0.02	0.515TrpThr: 0.515 ± 0.018	0.641TrpVal: 0.641 ± 0.024	0.13TrpTrp: 0.13 ± 0.01	0.343TrpTyr: 0.343 ± 0.018	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.162TyrAla: 2.162 ± 0.038	0.309TyrCys: 0.309 ± 0.016	1.896TyrAsp: 1.896 ± 0.04	2.565TyrGlu: 2.565 ± 0.043	1.879TyrPhe: 1.879 ± 0.037	2.487TyrGly: 2.487 ± 0.046	0.817TyrHis: 0.817 ± 0.025	2.526TyrIle: 2.526 ± 0.043	2.255TyrLys: 2.255 ± 0.044	3.428TyrLeu: 3.428 ± 0.054	0.901TyrMet: 0.901 ± 0.024	1.43TyrAsn: 1.43 ± 0.034	1.429TyrPro: 1.429 ± 0.03	1.427TyrGln: 1.427 ± 0.031	1.636TyrArg: 1.636 ± 0.035	2.271TyrSer: 2.271 ± 0.041	1.803TyrThr: 1.803 ± 0.041	1.99TyrVal: 1.99 ± 0.035	0.412TyrTrp: 0.412 ± 0.018	1.434TyrTyr: 1.434 ± 0.034	0.001TyrXaa: 0.001 ± 0.001
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.001XaaGlu: 0.001 ± 0.001	0.001XaaPhe: 0.001 ± 0.001	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.001XaaIle: 0.001 ± 0.001	0.001XaaLys: 0.001 ± 0.001	0.001XaaLeu: 0.001 ± 0.001	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.001XaaGln: 0.001 ± 0.001	0.0XaaArg: 0.0 ± 0.0	0.001XaaSer: 0.001 ± 0.001	0.001XaaThr: 0.001 ± 0.001	0.001XaaVal: 0.001 ± 0.001	0.001XaaTrp: 0.001 ± 0.001	0.001XaaTyr: 0.001 ± 0.001	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 5125 proteins (1462172 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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