Acetobacter senegalensis
Average proteome isoelectric point is 6.75
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3497 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0U5F0P5|A0A0U5F0P5_9PROT GCN5-related N-acetyltransferase OS=Acetobacter senegalensis OX=446692 GN=ASN_2227 PE=4 SV=1
MM1 pKa = 7.9 PFQNQVNQEE10 pKa = 3.92 LAYY13 pKa = 10.09 GVPGTFASNNPDD25 pKa = 3.08 ASAVPPEE32 pKa = 4.1 GAYY35 pKa = 10.17 VAGTGGCTIAAFGWDD50 pKa = 3.46 QGDD53 pKa = 3.78 GTVLNAPPASTTSYY67 pKa = 10.15 TVTALAVGAGGTGYY81 pKa = 11.27 AVGDD85 pKa = 3.65 TAAFAGGKK93 pKa = 8.3 ATVSTIGTGGVVTAVTLQSATAQSTDD119 pKa = 3.13 PTATGVATTTNGSGSGLTLNVTGTSSTTAAGAPTGLVFNDD159 pKa = 3.57 RR160 pKa = 11.84 SAWISDD166 pKa = 3.35 IYY168 pKa = 11.5 DD169 pKa = 3.64 EE170 pKa = 4.79 ATMVMPQGYY179 pKa = 7.75 MVDD182 pKa = 3.87 LKK184 pKa = 10.46 TAGDD188 pKa = 3.83 YY189 pKa = 9.94 FAAATTAATAGQKK202 pKa = 9.84 VFASTTDD209 pKa = 3.41 GTLSTGAAGATVTGAVEE226 pKa = 4.07 TNFYY230 pKa = 8.8 VTLGGSAGEE239 pKa = 4.51 TITISTWSRR248 pKa = 11.84 GG249 pKa = 3.51
Molecular weight: 24.25 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.805
IPC2_protein 3.732
IPC_protein 3.681
Toseland 3.465
ProMoST 3.884
Dawson 3.694
Bjellqvist 3.859
Wikipedia 3.668
Rodwell 3.516
Grimsley 3.389
Solomon 3.668
Lehninger 3.63
Nozaki 3.834
DTASelect 4.075
Thurlkill 3.554
EMBOSS 3.668
Sillero 3.808
Patrickios 0.299
IPC_peptide 3.668
IPC2_peptide 3.783
IPC2.peptide.svr19 3.752
Protein with the highest isoelectric point:
>tr|A0A0U4Y6H5|A0A0U4Y6H5_9PROT Aminopeptidase OS=Acetobacter senegalensis OX=446692 GN=pepN PE=3 SV=1
MM1 pKa = 7.35 KK2 pKa = 9.4 RR3 pKa = 11.84 TYY5 pKa = 10.2 QPSRR9 pKa = 11.84 LVRR12 pKa = 11.84 KK13 pKa = 9.21 RR14 pKa = 11.84 RR15 pKa = 11.84 HH16 pKa = 4.48 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MATVGGRR28 pKa = 11.84 RR29 pKa = 11.84 IIANRR34 pKa = 11.84 RR35 pKa = 11.84 SKK37 pKa = 10.47 GRR39 pKa = 11.84 KK40 pKa = 8.72 RR41 pKa = 11.84 LSAA44 pKa = 3.96
Molecular weight: 5.24 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.463
IPC2_protein 11.184
IPC_protein 12.588
Toseland 12.749
ProMoST 13.247
Dawson 12.749
Bjellqvist 12.749
Wikipedia 13.217
Rodwell 12.384
Grimsley 12.793
Solomon 13.247
Lehninger 13.144
Nozaki 12.749
DTASelect 12.749
Thurlkill 12.749
EMBOSS 13.247
Sillero 12.749
Patrickios 12.106
IPC_peptide 13.247
IPC2_peptide 12.237
IPC2.peptide.svr19 9.106
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3497
0
3497
1106584
19
2854
316.4
34.46
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
11.498 ± 0.056
1.057 ± 0.014
5.262 ± 0.031
5.162 ± 0.042
3.655 ± 0.025
8.171 ± 0.038
2.422 ± 0.02
4.847 ± 0.035
3.427 ± 0.033
10.216 ± 0.051
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.535 ± 0.019
2.922 ± 0.033
5.558 ± 0.038
3.786 ± 0.027
6.556 ± 0.039
6.018 ± 0.037
5.933 ± 0.029
7.124 ± 0.036
1.448 ± 0.016
2.404 ± 0.028
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here