Bosea sp. TND4EK4

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales; Boseaceae; Bosea; unclassified Bosea

Average proteome isoelectric point is 6.9

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4420 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1N6NCS0|A0A1N6NCS0_9BRAD Uncharacterized protein OS=Bosea sp. TND4EK4 OX=1907408 GN=SAMN05880592_10183 PE=4 SV=1
MM1 pKa = 7.74RR2 pKa = 11.84GDD4 pKa = 3.76LSGTHH9 pKa = 6.07QYY11 pKa = 11.21TIMEE15 pKa = 4.85CVMTMHH21 pKa = 6.77YY22 pKa = 9.14PPPPPPPAADD32 pKa = 3.33PHH34 pKa = 5.83QLQGQAQGEE43 pKa = 4.31LQGQGQGQLQGQGSYY58 pKa = 10.08QGQSQGQYY66 pKa = 10.12AGQAIDD72 pKa = 3.44TSVWNSACNEE82 pKa = 3.94NANEE86 pKa = 3.91NGNLNGNANLNGNLNLNDD104 pKa = 4.34NINHH108 pKa = 6.3NEE110 pKa = 4.1VQNHH114 pKa = 5.19VEE116 pKa = 4.21NTVDD120 pKa = 3.3TAVNVCVNVDD130 pKa = 3.93LGVDD134 pKa = 3.82LSCYY138 pKa = 10.37VPADD142 pKa = 3.57NDD144 pKa = 4.41AIDD147 pKa = 4.03IDD149 pKa = 4.96SICGTTNSIIMPDD162 pKa = 3.45VVSQSVTNGNAFNLDD177 pKa = 3.44QVSNLTDD184 pKa = 3.54NDD186 pKa = 4.12SLSNPSVAFNAGGVDD201 pKa = 3.42SSGWCGVNPDD211 pKa = 3.62AAGDD215 pKa = 3.78FSMTATATGGAATSSIGDD233 pKa = 3.37ITGDD237 pKa = 3.21HH238 pKa = 6.29ATQTGIGASTAAASLTQEE256 pKa = 4.94AFTQHH261 pKa = 5.64IAMGANIQFNQLDD274 pKa = 3.41IHH276 pKa = 6.06VAHH279 pKa = 7.64DD280 pKa = 3.6ISDD283 pKa = 3.85SLTGG287 pKa = 3.54

Molecular weight: 29.66 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1N6VC36|A0A1N6VC36_9BRAD Glycerol-3-phosphate dehydrogenase OS=Bosea sp. TND4EK4 OX=1907408 GN=SAMN05880592_105141 PE=3 SV=1
MM1 pKa = 6.8SHH3 pKa = 6.63IVIVLLVAAFIFRR16 pKa = 11.84KK17 pKa = 9.55PLGRR21 pKa = 11.84LLARR25 pKa = 11.84QFPNRR30 pKa = 11.84AKK32 pKa = 9.74RR33 pKa = 11.84QQVMGTVIAAFLLVIALRR51 pKa = 11.84LLALFWW57 pKa = 4.59

Molecular weight: 6.5 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4420

0

4420

1398793

26

2153

316.5

34.12

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.551 ± 0.054

0.786 ± 0.009

5.367 ± 0.034

5.623 ± 0.038

3.598 ± 0.025

8.889 ± 0.035

1.912 ± 0.019

5.016 ± 0.026

3.206 ± 0.03

10.491 ± 0.044

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.374 ± 0.015

2.246 ± 0.02

5.448 ± 0.028

3.091 ± 0.02

7.445 ± 0.042

5.205 ± 0.026

5.054 ± 0.022

7.381 ± 0.028

1.3 ± 0.013

2.017 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski