Amino acid dipepetide frequency for Bosea sp. TND4EK4

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
19.878AlaAla: 19.878 ± 0.18
1.175AlaCys: 1.175 ± 0.029
6.666AlaAsp: 6.666 ± 0.077
8.247AlaGlu: 8.247 ± 0.085
4.834AlaPhe: 4.834 ± 0.065
12.241AlaGly: 12.241 ± 0.131
2.261AlaHis: 2.261 ± 0.041
7.009AlaIle: 7.009 ± 0.09
4.413AlaLys: 4.413 ± 0.078
15.25AlaLeu: 15.25 ± 0.156
3.84AlaMet: 3.84 ± 0.05
2.694AlaAsn: 2.694 ± 0.048
6.344AlaPro: 6.344 ± 0.091
4.423AlaGln: 4.423 ± 0.062
9.899AlaArg: 9.899 ± 0.102
6.794AlaSer: 6.794 ± 0.076
6.317AlaThr: 6.317 ± 0.08
9.1AlaVal: 9.1 ± 0.085
1.568AlaTrp: 1.568 ± 0.034
2.553AlaTyr: 2.553 ± 0.049
0.0AlaXaa: 0.0 ± 0.0
Cys
0.987CysAla: 0.987 ± 0.024
0.102CysCys: 0.102 ± 0.01
0.466CysAsp: 0.466 ± 0.016
0.431CysGlu: 0.431 ± 0.017
0.28CysPhe: 0.28 ± 0.014
0.906CysGly: 0.906 ± 0.025
0.219CysHis: 0.219 ± 0.013
0.372CysIle: 0.372 ± 0.016
0.171CysLys: 0.171 ± 0.011
0.775CysLeu: 0.775 ± 0.026
0.137CysMet: 0.137 ± 0.012
0.167CysAsn: 0.167 ± 0.012
0.412CysPro: 0.412 ± 0.019
0.188CysGln: 0.188 ± 0.012
0.611CysArg: 0.611 ± 0.023
0.423CysSer: 0.423 ± 0.019
0.354CysThr: 0.354 ± 0.015
0.563CysVal: 0.563 ± 0.019
0.113CysTrp: 0.113 ± 0.009
0.186CysTyr: 0.186 ± 0.012
0.0CysXaa: 0.0 ± 0.0
Asp
6.932AspAla: 6.932 ± 0.08
0.401AspCys: 0.401 ± 0.021
2.808AspAsp: 2.808 ± 0.062
3.356AspGlu: 3.356 ± 0.051
2.002AspPhe: 2.002 ± 0.043
5.109AspGly: 5.109 ± 0.084
1.13AspHis: 1.13 ± 0.033
2.815AspIle: 2.815 ± 0.045
1.669AspLys: 1.669 ± 0.039
5.823AspLeu: 5.823 ± 0.076
1.184AspMet: 1.184 ± 0.028
1.015AspAsn: 1.015 ± 0.029
3.478AspPro: 3.478 ± 0.056
1.466AspGln: 1.466 ± 0.038
4.161AspArg: 4.161 ± 0.061
1.962AspSer: 1.962 ± 0.04
2.269AspThr: 2.269 ± 0.041
3.879AspVal: 3.879 ± 0.064
0.933AspTrp: 0.933 ± 0.028
1.28AspTyr: 1.28 ± 0.03
0.0AspXaa: 0.0 ± 0.0
Glu
8.595GluAla: 8.595 ± 0.094
0.319GluCys: 0.319 ± 0.014
2.484GluAsp: 2.484 ± 0.045
2.977GluGlu: 2.977 ± 0.055
1.541GluPhe: 1.541 ± 0.036
4.429GluGly: 4.429 ± 0.06
1.122GluHis: 1.122 ± 0.031
3.444GluIle: 3.444 ± 0.059
2.071GluLys: 2.071 ± 0.047
5.295GluLeu: 5.295 ± 0.062
1.432GluMet: 1.432 ± 0.033
1.316GluAsn: 1.316 ± 0.032
3.035GluPro: 3.035 ± 0.054
2.045GluGln: 2.045 ± 0.039
5.413GluArg: 5.413 ± 0.072
2.242GluSer: 2.242 ± 0.039
3.402GluThr: 3.402 ± 0.055
3.69GluVal: 3.69 ± 0.055
0.619GluTrp: 0.619 ± 0.021
0.761GluTyr: 0.761 ± 0.023
0.0GluXaa: 0.0 ± 0.0
Phe
4.92PheAla: 4.92 ± 0.059
0.382PheCys: 0.382 ± 0.017
2.456PheAsp: 2.456 ± 0.049
2.029PheGlu: 2.029 ± 0.036
1.304PhePhe: 1.304 ± 0.038
3.74PheGly: 3.74 ± 0.047
0.718PheHis: 0.718 ± 0.023
1.564PheIle: 1.564 ± 0.036
0.987PheLys: 0.987 ± 0.028
3.228PheLeu: 3.228 ± 0.06
0.785PheMet: 0.785 ± 0.026
0.88PheAsn: 0.88 ± 0.025
1.627PhePro: 1.627 ± 0.036
0.962PheGln: 0.962 ± 0.026
2.333PheArg: 2.333 ± 0.04
2.079PheSer: 2.079 ± 0.045
1.826PheThr: 1.826 ± 0.04
2.848PheVal: 2.848 ± 0.053
0.53PheTrp: 0.53 ± 0.02
0.781PheTyr: 0.781 ± 0.028
0.0PheXaa: 0.0 ± 0.0
Gly
10.205GlyAla: 10.205 ± 0.113
0.846GlyCys: 0.846 ± 0.025
4.252GlyAsp: 4.252 ± 0.067
5.147GlyGlu: 5.147 ± 0.074
3.77GlyPhe: 3.77 ± 0.058
7.737GlyGly: 7.737 ± 0.107
1.971GlyHis: 1.971 ± 0.044
4.517GlyIle: 4.517 ± 0.06
3.284GlyLys: 3.284 ± 0.054
9.972GlyLeu: 9.972 ± 0.113
2.235GlyMet: 2.235 ± 0.038
1.927GlyAsn: 1.927 ± 0.049
3.908GlyPro: 3.908 ± 0.058
3.0GlyGln: 3.0 ± 0.053
6.978GlyArg: 6.978 ± 0.077
4.828GlySer: 4.828 ± 0.075
4.542GlyThr: 4.542 ± 0.076
6.068GlyVal: 6.068 ± 0.074
1.45GlyTrp: 1.45 ± 0.037
2.256GlyTyr: 2.256 ± 0.037
0.0GlyXaa: 0.0 ± 0.0
His
2.449HisAla: 2.449 ± 0.044
0.218HisCys: 0.218 ± 0.011
1.205HisAsp: 1.205 ± 0.031
1.001HisGlu: 1.001 ± 0.034
0.748HisPhe: 0.748 ± 0.021
1.956HisGly: 1.956 ± 0.044
0.525HisHis: 0.525 ± 0.024
0.805HisIle: 0.805 ± 0.024
0.433HisLys: 0.433 ± 0.018
2.015HisLeu: 2.015 ± 0.043
0.415HisMet: 0.415 ± 0.016
0.381HisAsn: 0.381 ± 0.017
1.248HisPro: 1.248 ± 0.03
0.509HisGln: 0.509 ± 0.022
1.409HisArg: 1.409 ± 0.032
0.821HisSer: 0.821 ± 0.025
0.688HisThr: 0.688 ± 0.022
1.5HisVal: 1.5 ± 0.035
0.318HisTrp: 0.318 ± 0.016
0.478HisTyr: 0.478 ± 0.018
0.0HisXaa: 0.0 ± 0.0
Ile
7.726IleAla: 7.726 ± 0.084
0.482IleCys: 0.482 ± 0.018
3.438IleAsp: 3.438 ± 0.048
3.438IleGlu: 3.438 ± 0.057
1.506IlePhe: 1.506 ± 0.033
5.258IleGly: 5.258 ± 0.067
0.856IleHis: 0.856 ± 0.027
1.962IleIle: 1.962 ± 0.042
1.366IleLys: 1.366 ± 0.042
4.378IleLeu: 4.378 ± 0.065
0.909IleMet: 0.909 ± 0.026
1.122IleAsn: 1.122 ± 0.028
2.271IlePro: 2.271 ± 0.038
1.109IleGln: 1.109 ± 0.029
3.216IleArg: 3.216 ± 0.048
2.489IleSer: 2.489 ± 0.048
2.334IleThr: 2.334 ± 0.04
4.643IleVal: 4.643 ± 0.065
0.619IleTrp: 0.619 ± 0.022
1.037IleTyr: 1.037 ± 0.026
0.0IleXaa: 0.0 ± 0.0
Lys
4.911LysAla: 4.911 ± 0.062
0.117LysCys: 0.117 ± 0.01
1.601LysAsp: 1.601 ± 0.046
1.473LysGlu: 1.473 ± 0.034
0.749LysPhe: 0.749 ± 0.023
2.77LysGly: 2.77 ± 0.051
0.518LysHis: 0.518 ± 0.021
1.471LysIle: 1.471 ± 0.034
1.131LysLys: 1.131 ± 0.042
3.509LysLeu: 3.509 ± 0.057
0.613LysMet: 0.613 ± 0.025
0.716LysAsn: 0.716 ± 0.024
2.391LysPro: 2.391 ± 0.048
0.969LysGln: 0.969 ± 0.03
2.474LysArg: 2.474 ± 0.046
1.619LysSer: 1.619 ± 0.038
1.802LysThr: 1.802 ± 0.036
2.433LysVal: 2.433 ± 0.046
0.295LysTrp: 0.295 ± 0.015
0.496LysTyr: 0.496 ± 0.021
0.0LysXaa: 0.0 ± 0.0
Leu
15.653LeuAla: 15.653 ± 0.149
0.876LeuCys: 0.876 ± 0.027
6.285LeuAsp: 6.285 ± 0.074
4.881LeuGlu: 4.881 ± 0.069
3.57LeuPhe: 3.57 ± 0.056
9.259LeuGly: 9.259 ± 0.099
1.727LeuHis: 1.727 ± 0.038
4.967LeuIle: 4.967 ± 0.071
3.847LeuLys: 3.847 ± 0.048
10.011LeuLeu: 10.011 ± 0.13
2.288LeuMet: 2.288 ± 0.048
2.3LeuAsn: 2.3 ± 0.038
6.077LeuPro: 6.077 ± 0.072
2.721LeuGln: 2.721 ± 0.05
7.266LeuArg: 7.266 ± 0.075
6.451LeuSer: 6.451 ± 0.066
5.448LeuThr: 5.448 ± 0.07
8.177LeuVal: 8.177 ± 0.082
1.201LeuTrp: 1.201 ± 0.033
1.904LeuTyr: 1.904 ± 0.037
0.0LeuXaa: 0.0 ± 0.0
Met
3.201MetAla: 3.201 ± 0.05
0.148MetCys: 0.148 ± 0.011
0.982MetAsp: 0.982 ± 0.028
0.928MetGlu: 0.928 ± 0.028
0.59MetPhe: 0.59 ± 0.023
1.736MetGly: 1.736 ± 0.036
0.367MetHis: 0.367 ± 0.017
1.252MetIle: 1.252 ± 0.033
0.932MetLys: 0.932 ± 0.031
2.613MetLeu: 2.613 ± 0.046
0.614MetMet: 0.614 ± 0.021
0.689MetAsn: 0.689 ± 0.023
1.568MetPro: 1.568 ± 0.039
0.781MetGln: 0.781 ± 0.025
1.845MetArg: 1.845 ± 0.034
1.601MetSer: 1.601 ± 0.036
1.892MetThr: 1.892 ± 0.037
1.59MetVal: 1.59 ± 0.034
0.168MetTrp: 0.168 ± 0.012
0.244MetTyr: 0.244 ± 0.014
0.0MetXaa: 0.0 ± 0.0
Asn
2.964AsnAla: 2.964 ± 0.047
0.189AsnCys: 0.189 ± 0.011
1.217AsnAsp: 1.217 ± 0.038
1.119AsnGlu: 1.119 ± 0.029
0.787AsnPhe: 0.787 ± 0.026
2.143AsnGly: 2.143 ± 0.042
0.437AsnHis: 0.437 ± 0.017
1.104AsnIle: 1.104 ± 0.027
0.588AsnLys: 0.588 ± 0.025
2.323AsnLeu: 2.323 ± 0.037
0.474AsnMet: 0.474 ± 0.018
0.572AsnAsn: 0.572 ± 0.02
1.658AsnPro: 1.658 ± 0.038
0.621AsnGln: 0.621 ± 0.02
1.618AsnArg: 1.618 ± 0.036
1.02AsnSer: 1.02 ± 0.03
1.083AsnThr: 1.083 ± 0.028
1.639AsnVal: 1.639 ± 0.037
0.378AsnTrp: 0.378 ± 0.016
0.524AsnTyr: 0.524 ± 0.021
0.0AsnXaa: 0.0 ± 0.0
Pro
7.617ProAla: 7.617 ± 0.096
0.322ProCys: 0.322 ± 0.016
3.432ProAsp: 3.432 ± 0.052
3.723ProGlu: 3.723 ± 0.049
2.114ProPhe: 2.114 ± 0.04
5.026ProGly: 5.026 ± 0.058
1.081ProHis: 1.081 ± 0.029
2.338ProIle: 2.338 ± 0.042
1.701ProLys: 1.701 ± 0.039
5.119ProLeu: 5.119 ± 0.065
1.238ProMet: 1.238 ± 0.031
1.255ProAsn: 1.255 ± 0.034
2.82ProPro: 2.82 ± 0.059
1.867ProGln: 1.867 ± 0.045
3.356ProArg: 3.356 ± 0.057
2.923ProSer: 2.923 ± 0.042
2.392ProThr: 2.392 ± 0.042
4.374ProVal: 4.374 ± 0.059
0.701ProTrp: 0.701 ± 0.025
1.078ProTyr: 1.078 ± 0.029
0.0ProXaa: 0.0 ± 0.0
Gln
4.632GlnAla: 4.632 ± 0.064
0.141GlnCys: 0.141 ± 0.009
1.476GlnAsp: 1.476 ± 0.039
1.498GlnGlu: 1.498 ± 0.031
0.934GlnPhe: 0.934 ± 0.029
2.533GlnGly: 2.533 ± 0.043
0.626GlnHis: 0.626 ± 0.022
1.819GlnIle: 1.819 ± 0.038
0.982GlnLys: 0.982 ± 0.027
2.673GlnLeu: 2.673 ± 0.044
0.799GlnMet: 0.799 ± 0.027
0.794GlnAsn: 0.794 ± 0.025
1.935GlnPro: 1.935 ± 0.038
1.219GlnGln: 1.219 ± 0.034
2.628GlnArg: 2.628 ± 0.049
1.626GlnSer: 1.626 ± 0.034
1.606GlnThr: 1.606 ± 0.033
2.151GlnVal: 2.151 ± 0.042
0.325GlnTrp: 0.325 ± 0.014
0.513GlnTyr: 0.513 ± 0.021
0.0GlnXaa: 0.0 ± 0.0
Arg
8.299ArgAla: 8.299 ± 0.088
0.522ArgCys: 0.522 ± 0.02
4.146ArgAsp: 4.146 ± 0.057
4.545ArgGlu: 4.545 ± 0.058
3.169ArgPhe: 3.169 ± 0.047
5.182ArgGly: 5.182 ± 0.061
1.697ArgHis: 1.697 ± 0.033
4.244ArgIle: 4.244 ± 0.057
2.364ArgLys: 2.364 ± 0.043
8.756ArgLeu: 8.756 ± 0.087
1.942ArgMet: 1.942 ± 0.039
1.782ArgAsn: 1.782 ± 0.032
4.052ArgPro: 4.052 ± 0.058
2.729ArgGln: 2.729 ± 0.046
6.354ArgArg: 6.354 ± 0.09
3.923ArgSer: 3.923 ± 0.061
3.229ArgThr: 3.229 ± 0.059
4.738ArgVal: 4.738 ± 0.06
1.051ArgTrp: 1.051 ± 0.027
1.721ArgTyr: 1.721 ± 0.036
0.0ArgXaa: 0.0 ± 0.0
Ser
6.421SerAla: 6.421 ± 0.066
0.41SerCys: 0.41 ± 0.018
2.627SerAsp: 2.627 ± 0.049
2.762SerGlu: 2.762 ± 0.043
2.321SerPhe: 2.321 ± 0.048
5.396SerGly: 5.396 ± 0.066
1.001SerHis: 1.001 ± 0.026
2.527SerIle: 2.527 ± 0.044
1.38SerLys: 1.38 ± 0.032
5.598SerLeu: 5.598 ± 0.069
1.178SerMet: 1.178 ± 0.025
1.14SerAsn: 1.14 ± 0.029
2.79SerPro: 2.79 ± 0.046
1.637SerGln: 1.637 ± 0.043
3.781SerArg: 3.781 ± 0.057
2.722SerSer: 2.722 ± 0.056
2.472SerThr: 2.472 ± 0.044
3.855SerVal: 3.855 ± 0.047
0.804SerTrp: 0.804 ± 0.022
1.224SerTyr: 1.224 ± 0.029
0.0SerXaa: 0.0 ± 0.0
Thr
6.253ThrAla: 6.253 ± 0.067
0.366ThrCys: 0.366 ± 0.017
2.394ThrAsp: 2.394 ± 0.049
2.407ThrGlu: 2.407 ± 0.045
1.816ThrPhe: 1.816 ± 0.038
4.994ThrGly: 4.994 ± 0.067
0.894ThrHis: 0.894 ± 0.029
2.855ThrIle: 2.855 ± 0.042
1.37ThrLys: 1.37 ± 0.035
6.006ThrLeu: 6.006 ± 0.073
1.14ThrMet: 1.14 ± 0.03
1.117ThrAsn: 1.117 ± 0.032
3.069ThrPro: 3.069 ± 0.045
1.489ThrGln: 1.489 ± 0.032
3.353ThrArg: 3.353 ± 0.055
2.536ThrSer: 2.536 ± 0.039
2.69ThrThr: 2.69 ± 0.048
4.199ThrVal: 4.199 ± 0.06
0.548ThrTrp: 0.548 ± 0.021
1.045ThrTyr: 1.045 ± 0.029
0.0ThrXaa: 0.0 ± 0.0
Val
10.144ValAla: 10.144 ± 0.088
0.55ValCys: 0.55 ± 0.019
3.862ValAsp: 3.862 ± 0.055
4.603ValGlu: 4.603 ± 0.059
2.653ValPhe: 2.653 ± 0.047
5.524ValGly: 5.524 ± 0.07
1.277ValHis: 1.277 ± 0.032
3.764ValIle: 3.764 ± 0.061
2.4ValLys: 2.4 ± 0.049
7.695ValLeu: 7.695 ± 0.09
1.857ValMet: 1.857 ± 0.037
1.72ValAsn: 1.72 ± 0.034
3.979ValPro: 3.979 ± 0.048
1.98ValGln: 1.98 ± 0.036
4.785ValArg: 4.785 ± 0.066
4.232ValSer: 4.232 ± 0.063
4.53ValThr: 4.53 ± 0.064
6.112ValVal: 6.112 ± 0.087
0.809ValTrp: 0.809 ± 0.028
1.337ValTyr: 1.337 ± 0.03
0.0ValXaa: 0.0 ± 0.0
Trp
1.24TrpAla: 1.24 ± 0.031
0.132TrpCys: 0.132 ± 0.01
0.555TrpAsp: 0.555 ± 0.021
0.503TrpGlu: 0.503 ± 0.018
0.523TrpPhe: 0.523 ± 0.021
0.911TrpGly: 0.911 ± 0.027
0.308TrpHis: 0.308 ± 0.015
0.593TrpIle: 0.593 ± 0.02
0.421TrpLys: 0.421 ± 0.018
1.768TrpLeu: 1.768 ± 0.046
0.31TrpMet: 0.31 ± 0.013
0.384TrpAsn: 0.384 ± 0.02
0.735TrpPro: 0.735 ± 0.025
0.57TrpGln: 0.57 ± 0.02
1.247TrpArg: 1.247 ± 0.034
0.811TrpSer: 0.811 ± 0.028
0.78TrpThr: 0.78 ± 0.026
0.726TrpVal: 0.726 ± 0.025
0.227TrpTrp: 0.227 ± 0.014
0.255TrpTyr: 0.255 ± 0.016
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.481TyrAla: 2.481 ± 0.045
0.168TyrCys: 0.168 ± 0.01
1.323TyrAsp: 1.323 ± 0.033
1.161TyrGlu: 1.161 ± 0.027
0.769TyrPhe: 0.769 ± 0.026
2.042TyrGly: 2.042 ± 0.042
0.386TyrHis: 0.386 ± 0.016
0.737TyrIle: 0.737 ± 0.022
0.546TyrLys: 0.546 ± 0.021
2.1TyrLeu: 2.1 ± 0.04
0.35TyrMet: 0.35 ± 0.015
0.487TyrAsn: 0.487 ± 0.02
1.08TyrPro: 1.08 ± 0.028
0.624TyrGln: 0.624 ± 0.021
1.72TyrArg: 1.72 ± 0.035
0.942TyrSer: 0.942 ± 0.027
0.886TyrThr: 0.886 ± 0.023
1.525TyrVal: 1.525 ± 0.036
0.345TyrTrp: 0.345 ± 0.017
0.5TyrTyr: 0.5 ± 0.021
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 4420 proteins (1398793 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski