Pricia antarctica
Average proteome isoelectric point is 6.43
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4172 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1G6ZS75|A0A1G6ZS75_9FLAO Uncharacterized protein OS=Pricia antarctica OX=641691 GN=SAMN05421636_10377 PE=4 SV=1
MM1 pKa = 7.76 KK2 pKa = 10.17 IGVLIIGIFFMGNYY16 pKa = 10.34 ALFGQITVDD25 pKa = 3.28 DD26 pKa = 3.71 SGYY29 pKa = 9.06 TVEE32 pKa = 4.23 QLVRR36 pKa = 11.84 DD37 pKa = 3.68 VLINSNCAATSSYY50 pKa = 11.58 SSFTGTEE57 pKa = 3.8 QNVNGIGYY65 pKa = 9.38 FNANATDD72 pKa = 4.07 FPYY75 pKa = 10.73 KK76 pKa = 10.35 EE77 pKa = 4.73 GIILSTGRR85 pKa = 11.84 ARR87 pKa = 11.84 DD88 pKa = 3.56 AQGPNTGINDD98 pKa = 3.89 SGTEE102 pKa = 4.01 SWPGDD107 pKa = 3.27 QDD109 pKa = 5.54 LITVTNTGNLFNATYY124 pKa = 9.92 IQFDD128 pKa = 4.21 FVPLTNSINFNFLFASEE145 pKa = 5.16 EE146 pKa = 4.03 YY147 pKa = 10.3 QEE149 pKa = 5.66 NYY151 pKa = 8.85 QCTYY155 pKa = 9.97 SDD157 pKa = 3.39 VFAFILTDD165 pKa = 3.51 SQGVSTNLAIVPGTEE180 pKa = 3.81 EE181 pKa = 4.11 PVRR184 pKa = 11.84 ATTIRR189 pKa = 11.84 PGIQGQCDD197 pKa = 3.28 ARR199 pKa = 11.84 NIDD202 pKa = 4.83 FFGKK206 pKa = 10.52 LNGANDD212 pKa = 4.88 PISFYY217 pKa = 11.36 GQTEE221 pKa = 4.26 SLKK224 pKa = 10.92 AEE226 pKa = 4.18 SLVTPGEE233 pKa = 4.32 SYY235 pKa = 10.28 TIKK238 pKa = 10.78 LVISDD243 pKa = 4.12 NQDD246 pKa = 3.18 SQVDD250 pKa = 3.72 SAVFLEE256 pKa = 4.74 AGSFSLGYY264 pKa = 10.5 DD265 pKa = 3.1 LGEE268 pKa = 4.72 DD269 pKa = 2.89 RR270 pKa = 11.84 TVANGNPACIGEE282 pKa = 4.72 NIPLDD287 pKa = 3.56 ATVEE291 pKa = 4.33 GVQDD295 pKa = 3.56 YY296 pKa = 10.56 RR297 pKa = 11.84 WYY299 pKa = 10.86 KK300 pKa = 10.72 DD301 pKa = 3.29 GSEE304 pKa = 4.18 VAQWAGNPQVTLTEE318 pKa = 4.15 NGQYY322 pKa = 10.1 RR323 pKa = 11.84 VDD325 pKa = 5.02 LIFSASCISDD335 pKa = 3.1 GGLTVEE341 pKa = 5.8 FIVPPKK347 pKa = 10.57 INGNPVDD354 pKa = 3.88 ITACDD359 pKa = 3.85 LDD361 pKa = 5.15 GDD363 pKa = 4.17 GSEE366 pKa = 4.56 TFDD369 pKa = 4.02 FSTNGTRR376 pKa = 11.84 MMGTQDD382 pKa = 3.16 SAVYY386 pKa = 9.83 GVTYY390 pKa = 9.88 FASEE394 pKa = 3.42 VDD396 pKa = 3.48 AQNFTNPIEE405 pKa = 4.34 NPEE408 pKa = 3.98 NYY410 pKa = 8.96 EE411 pKa = 3.99 VQGTEE416 pKa = 3.8 TIYY419 pKa = 11.26 ARR421 pKa = 11.84 LSSGQSCSEE430 pKa = 3.64 IVPFQLKK437 pKa = 9.66 VQGLDD442 pKa = 3.55 FEE444 pKa = 5.16 SSLKK448 pKa = 10.6 ASYY451 pKa = 10.16 ILCFDD456 pKa = 3.87 GEE458 pKa = 4.67 GEE460 pKa = 4.15 KK461 pKa = 10.95 LEE463 pKa = 4.49 PLPVLDD469 pKa = 4.58 TGLSLSKK476 pKa = 11.42 YY477 pKa = 7.58 MFTWYY482 pKa = 9.59 TEE484 pKa = 4.41 TISEE488 pKa = 4.27 EE489 pKa = 3.91 NRR491 pKa = 11.84 ITDD494 pKa = 3.2 ATGPSFTASALGTYY508 pKa = 9.63 HH509 pKa = 7.21 VLLQNLEE516 pKa = 4.43 FGCEE520 pKa = 3.67 FSIATEE526 pKa = 4.09 VLPSQQPEE534 pKa = 4.08 VFEE537 pKa = 4.35 VDD539 pKa = 4.1 FVSDD543 pKa = 4.7 LFSDD547 pKa = 3.93 SNTVEE552 pKa = 3.99 IKK554 pKa = 10.68 AQGEE558 pKa = 4.43 STYY561 pKa = 11.15 LFAVDD566 pKa = 5.47 DD567 pKa = 4.34 SDD569 pKa = 5.03 FGTSNRR575 pKa = 11.84 FEE577 pKa = 4.57 DD578 pKa = 4.49 LSAGEE583 pKa = 4.05 HH584 pKa = 4.66 VAYY587 pKa = 9.56 VTDD590 pKa = 4.14 SEE592 pKa = 4.57 NCSVLSEE599 pKa = 4.57 DD600 pKa = 4.14 FLVVDD605 pKa = 5.02 FPRR608 pKa = 11.84 FFTPNGDD615 pKa = 3.64 GNNDD619 pKa = 2.2 IWQIVGFPEE628 pKa = 3.88 IEE630 pKa = 4.02 KK631 pKa = 11.05 AEE633 pKa = 3.95 IAIFDD638 pKa = 3.48 QYY640 pKa = 10.64 GTLLYY645 pKa = 10.53 QFNNGSGWDD654 pKa = 3.7 GTANDD659 pKa = 4.12 HH660 pKa = 6.93 RR661 pKa = 11.84 MPASDD666 pKa = 3.3 YY667 pKa = 8.7 WFRR670 pKa = 11.84 IAYY673 pKa = 9.94 VKK675 pKa = 11.12 DD676 pKa = 3.5 NVQKK680 pKa = 10.34 EE681 pKa = 4.62 FKK683 pKa = 10.39 SHH685 pKa = 6.98 FSLKK689 pKa = 10.32 RR690 pKa = 3.28
Molecular weight: 75.82 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.737
IPC2_protein 3.884
IPC_protein 3.897
Toseland 3.694
ProMoST 4.037
Dawson 3.872
Bjellqvist 4.024
Wikipedia 3.77
Rodwell 3.719
Grimsley 3.592
Solomon 3.872
Lehninger 3.821
Nozaki 3.973
DTASelect 4.19
Thurlkill 3.719
EMBOSS 3.783
Sillero 4.012
Patrickios 0.985
IPC_peptide 3.872
IPC2_peptide 3.999
IPC2.peptide.svr19 3.901
Protein with the highest isoelectric point:
>tr|A0A1G7DNU0|A0A1G7DNU0_9FLAO Uncharacterized protein OS=Pricia antarctica OX=641691 GN=SAMN05421636_105406 PE=4 SV=1
MM1 pKa = 7.47 LFVFDD6 pKa = 4.44 ICPKK10 pKa = 10.42 KK11 pKa = 10.67 SQTPNRR17 pKa = 11.84 AVWNLGFGTSIALGVIPLGLAATAVYY43 pKa = 9.4 WFIVNKK49 pKa = 10.22 NSSLFLVCLMRR60 pKa = 11.84 CSMNSIASTGFISAKK75 pKa = 7.3 YY76 pKa = 9.68 LRR78 pKa = 11.84 RR79 pKa = 11.84 IHH81 pKa = 5.96 MRR83 pKa = 3.27
Molecular weight: 9.26 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.34
IPC2_protein 9.677
IPC_protein 10.116
Toseland 10.496
ProMoST 10.452
Dawson 10.628
Bjellqvist 10.321
Wikipedia 10.789
Rodwell 11.008
Grimsley 10.672
Solomon 10.687
Lehninger 10.672
Nozaki 10.526
DTASelect 10.292
Thurlkill 10.511
EMBOSS 10.877
Sillero 10.555
Patrickios 10.833
IPC_peptide 10.687
IPC2_peptide 9.56
IPC2.peptide.svr19 8.504
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4172
0
4172
1420157
29
4883
340.4
38.25
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.924 ± 0.037
0.728 ± 0.013
5.972 ± 0.034
6.564 ± 0.029
5.041 ± 0.031
7.049 ± 0.039
1.896 ± 0.021
7.216 ± 0.03
7.168 ± 0.048
9.357 ± 0.049
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.404 ± 0.02
5.36 ± 0.034
3.76 ± 0.025
3.355 ± 0.02
3.985 ± 0.025
6.248 ± 0.031
5.696 ± 0.039
6.264 ± 0.027
1.161 ± 0.014
3.851 ± 0.024
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here