Amino acid dipepetide frequency for Strongylocentrotus purpuratus (Purple sea urchin)

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	5.192AlaAla: 5.192 ± 0.027	1.452AlaCys: 1.452 ± 0.026	3.479AlaAsp: 3.479 ± 0.018	4.119AlaGlu: 4.119 ± 0.022	2.263AlaPhe: 2.263 ± 0.014	4.06AlaGly: 4.06 ± 0.023	1.205AlaHis: 1.205 ± 0.008	3.212AlaIle: 3.212 ± 0.015	3.418AlaLys: 3.418 ± 0.022	5.317AlaLeu: 5.317 ± 0.024	1.659AlaMet: 1.659 ± 0.011	2.654AlaAsn: 2.654 ± 0.014	3.309AlaPro: 3.309 ± 0.027	2.516AlaGln: 2.516 ± 0.017	2.994AlaArg: 2.994 ± 0.015	5.754AlaSer: 5.754 ± 0.024	4.424AlaThr: 4.424 ± 0.029	4.351AlaVal: 4.351 ± 0.026	0.625AlaTrp: 0.625 ± 0.005	1.53AlaTyr: 1.53 ± 0.009	0.001AlaXaa: 0.001 ± 0.0
Cys	1.369CysAla: 1.369 ± 0.022	0.492CysCys: 0.492 ± 0.01	1.536CysAsp: 1.536 ± 0.019	1.569CysGlu: 1.569 ± 0.03	0.767CysPhe: 0.767 ± 0.008	1.57CysGly: 1.57 ± 0.026	0.535CysHis: 0.535 ± 0.006	1.172CysIle: 1.172 ± 0.015	1.01CysLys: 1.01 ± 0.01	1.892CysLeu: 1.892 ± 0.018	0.455CysMet: 0.455 ± 0.006	1.121CysAsn: 1.121 ± 0.017	1.436CysPro: 1.436 ± 0.03	1.077CysGln: 1.077 ± 0.021	1.226CysArg: 1.226 ± 0.013	2.443CysSer: 2.443 ± 0.05	1.391CysThr: 1.391 ± 0.016	1.305CysVal: 1.305 ± 0.014	0.206CysTrp: 0.206 ± 0.003	0.597CysTyr: 0.597 ± 0.009	0.0CysXaa: 0.0 ± 0.0
Asp	3.687AspAla: 3.687 ± 0.019	1.468AspCys: 1.468 ± 0.029	4.8AspAsp: 4.8 ± 0.032	4.662AspGlu: 4.662 ± 0.028	2.187AspPhe: 2.187 ± 0.014	4.605AspGly: 4.605 ± 0.033	1.341AspHis: 1.341 ± 0.009	3.776AspIle: 3.776 ± 0.032	2.922AspLys: 2.922 ± 0.017	5.036AspLeu: 5.036 ± 0.025	1.415AspMet: 1.415 ± 0.009	2.735AspAsn: 2.735 ± 0.026	3.167AspPro: 3.167 ± 0.032	2.258AspGln: 2.258 ± 0.014	2.839AspArg: 2.839 ± 0.018	4.61AspSer: 4.61 ± 0.023	3.228AspThr: 3.228 ± 0.018	4.097AspVal: 4.097 ± 0.022	0.624AspTrp: 0.624 ± 0.008	1.748AspTyr: 1.748 ± 0.015	0.001AspXaa: 0.001 ± 0.0
Glu	4.269GluAla: 4.269 ± 0.021	1.448GluCys: 1.448 ± 0.025	4.619GluAsp: 4.619 ± 0.025	7.073GluGlu: 7.073 ± 0.061	1.986GluPhe: 1.986 ± 0.013	4.246GluGly: 4.246 ± 0.021	1.33GluHis: 1.33 ± 0.01	3.27GluIle: 3.27 ± 0.018	4.454GluLys: 4.454 ± 0.035	5.165GluLeu: 5.165 ± 0.027	1.742GluMet: 1.742 ± 0.013	3.037GluAsn: 3.037 ± 0.023	2.693GluPro: 2.693 ± 0.023	2.648GluGln: 2.648 ± 0.017	3.778GluArg: 3.778 ± 0.024	4.697GluSer: 4.697 ± 0.027	3.837GluThr: 3.837 ± 0.023	4.18GluVal: 4.18 ± 0.022	0.664GluTrp: 0.664 ± 0.006	1.71GluTyr: 1.71 ± 0.013	0.001GluXaa: 0.001 ± 0.0
Phe	2.01PheAla: 2.01 ± 0.012	0.869PheCys: 0.869 ± 0.01	2.055PheAsp: 2.055 ± 0.013	1.968PheGlu: 1.968 ± 0.014	1.503PhePhe: 1.503 ± 0.017	2.345PheGly: 2.345 ± 0.017	0.87PheHis: 0.87 ± 0.008	2.005PheIle: 2.005 ± 0.013	1.723PheLys: 1.723 ± 0.012	3.088PheLeu: 3.088 ± 0.019	0.808PheMet: 0.808 ± 0.006	1.647PheAsn: 1.647 ± 0.014	1.626PhePro: 1.626 ± 0.013	1.447PheGln: 1.447 ± 0.011	1.687PheArg: 1.687 ± 0.011	2.963PheSer: 2.963 ± 0.016	2.632PheThr: 2.632 ± 0.034	2.241PheVal: 2.241 ± 0.014	0.418PheTrp: 0.418 ± 0.005	1.131PheTyr: 1.131 ± 0.009	0.001PheXaa: 0.001 ± 0.0
Gly	4.035GlyAla: 4.035 ± 0.026	1.189GlyCys: 1.189 ± 0.012	4.016GlyAsp: 4.016 ± 0.023	3.964GlyGlu: 3.964 ± 0.022	2.457GlyPhe: 2.457 ± 0.021	5.752GlyGly: 5.752 ± 0.061	1.784GlyHis: 1.784 ± 0.019	3.207GlyIle: 3.207 ± 0.024	3.377GlyLys: 3.377 ± 0.021	4.72GlyLeu: 4.72 ± 0.021	1.578GlyMet: 1.578 ± 0.014	3.241GlyAsn: 3.241 ± 0.027	3.016GlyPro: 3.016 ± 0.039	2.862GlyGln: 2.862 ± 0.02	3.562GlyArg: 3.562 ± 0.021	5.878GlySer: 5.878 ± 0.034	4.41GlyThr: 4.41 ± 0.04	4.134GlyVal: 4.134 ± 0.027	0.765GlyTrp: 0.765 ± 0.01	2.357GlyTyr: 2.357 ± 0.025	0.002GlyXaa: 0.002 ± 0.0
His	1.291HisAla: 1.291 ± 0.009	0.565HisCys: 0.565 ± 0.009	1.218HisAsp: 1.218 ± 0.009	1.262HisGlu: 1.262 ± 0.01	0.868HisPhe: 0.868 ± 0.008	1.502HisGly: 1.502 ± 0.012	1.022HisHis: 1.022 ± 0.011	1.231HisIle: 1.231 ± 0.01	1.16HisLys: 1.16 ± 0.009	2.368HisLeu: 2.368 ± 0.017	0.594HisMet: 0.594 ± 0.006	1.026HisAsn: 1.026 ± 0.01	1.393HisPro: 1.393 ± 0.011	1.242HisGln: 1.242 ± 0.012	1.39HisArg: 1.39 ± 0.012	1.985HisSer: 1.985 ± 0.014	1.445HisThr: 1.445 ± 0.016	1.455HisVal: 1.455 ± 0.012	0.255HisTrp: 0.255 ± 0.003	0.734HisTyr: 0.734 ± 0.007	0.0HisXaa: 0.0 ± 0.0
Ile	3.188IleAla: 3.188 ± 0.016	1.385IleCys: 1.385 ± 0.031	2.934IleAsp: 2.934 ± 0.015	2.888IleGlu: 2.888 ± 0.016	1.921IlePhe: 1.921 ± 0.012	2.939IleGly: 2.939 ± 0.016	1.232IleHis: 1.232 ± 0.01	2.754IleIle: 2.754 ± 0.018	2.609IleLys: 2.609 ± 0.015	4.321IleLeu: 4.321 ± 0.023	1.137IleMet: 1.137 ± 0.008	2.467IleAsn: 2.467 ± 0.022	2.824IlePro: 2.824 ± 0.015	2.229IleGln: 2.229 ± 0.013	2.475IleArg: 2.475 ± 0.014	4.087IleSer: 4.087 ± 0.018	3.353IleThr: 3.353 ± 0.024	3.127IleVal: 3.127 ± 0.017	0.461IleTrp: 0.461 ± 0.005	1.431IleTyr: 1.431 ± 0.011	0.001IleXaa: 0.001 ± 0.0
Lys	3.541LysAla: 3.541 ± 0.022	0.976LysCys: 0.976 ± 0.009	3.301LysAsp: 3.301 ± 0.022	4.573LysGlu: 4.573 ± 0.033	1.55LysPhe: 1.55 ± 0.01	3.185LysGly: 3.185 ± 0.02	1.325LysHis: 1.325 ± 0.01	2.454LysIle: 2.454 ± 0.015	4.699LysLys: 4.699 ± 0.043	4.529LysLeu: 4.529 ± 0.025	1.461LysMet: 1.461 ± 0.011	2.164LysAsn: 2.164 ± 0.011	2.799LysPro: 2.799 ± 0.026	2.481LysGln: 2.481 ± 0.017	3.409LysArg: 3.409 ± 0.019	3.933LysSer: 3.933 ± 0.023	3.163LysThr: 3.163 ± 0.019	3.29LysVal: 3.29 ± 0.019	0.585LysTrp: 0.585 ± 0.007	1.454LysTyr: 1.454 ± 0.011	0.001LysXaa: 0.001 ± 0.0
Leu	5.334LeuAla: 5.334 ± 0.022	1.763LeuCys: 1.763 ± 0.014	4.914LeuAsp: 4.914 ± 0.021	5.365LeuGlu: 5.365 ± 0.027	2.832LeuPhe: 2.832 ± 0.016	4.686LeuGly: 4.686 ± 0.023	2.202LeuHis: 2.202 ± 0.014	3.816LeuIle: 3.816 ± 0.017	4.853LeuLys: 4.853 ± 0.025	7.214LeuLeu: 7.214 ± 0.034	1.958LeuMet: 1.958 ± 0.012	3.623LeuAsn: 3.623 ± 0.016	4.571LeuPro: 4.571 ± 0.02	4.178LeuGln: 4.178 ± 0.021	4.474LeuArg: 4.474 ± 0.023	6.664LeuSer: 6.664 ± 0.029	5.05LeuThr: 5.05 ± 0.022	4.969LeuVal: 4.969 ± 0.019	0.812LeuTrp: 0.812 ± 0.008	2.228LeuTyr: 2.228 ± 0.013	0.001LeuXaa: 0.001 ± 0.0
Met	1.941MetAla: 1.941 ± 0.011	0.477MetCys: 0.477 ± 0.007	1.503MetAsp: 1.503 ± 0.011	1.848MetGlu: 1.848 ± 0.012	0.862MetPhe: 0.862 ± 0.009	1.368MetGly: 1.368 ± 0.011	0.495MetHis: 0.495 ± 0.006	1.047MetIle: 1.047 ± 0.009	1.514MetLys: 1.514 ± 0.011	1.836MetLeu: 1.836 ± 0.01	0.801MetMet: 0.801 ± 0.01	1.061MetAsn: 1.061 ± 0.009	1.239MetPro: 1.239 ± 0.012	1.006MetGln: 1.006 ± 0.009	1.162MetArg: 1.162 ± 0.008	1.936MetSer: 1.936 ± 0.012	1.617MetThr: 1.617 ± 0.016	1.469MetVal: 1.469 ± 0.01	0.247MetTrp: 0.247 ± 0.003	0.651MetTyr: 0.651 ± 0.006	0.0MetXaa: 0.0 ± 0.0
Asn	2.692AsnAla: 2.692 ± 0.015	1.049AsnCys: 1.049 ± 0.018	2.715AsnAsp: 2.715 ± 0.027	2.701AsnGlu: 2.701 ± 0.017	1.522AsnPhe: 1.522 ± 0.009	3.788AsnGly: 3.788 ± 0.039	1.019AsnHis: 1.019 ± 0.009	2.509AsnIle: 2.509 ± 0.014	2.231AsnLys: 2.231 ± 0.014	3.656AsnLeu: 3.656 ± 0.017	1.12AsnMet: 1.12 ± 0.01	2.181AsnAsn: 2.181 ± 0.018	2.626AsnPro: 2.626 ± 0.025	1.988AsnGln: 1.988 ± 0.015	2.12AsnArg: 2.12 ± 0.011	3.475AsnSer: 3.475 ± 0.02	2.804AsnThr: 2.804 ± 0.027	2.888AsnVal: 2.888 ± 0.018	0.418AsnTrp: 0.418 ± 0.006	1.22AsnTyr: 1.22 ± 0.01	0.0AsnXaa: 0.0 ± 0.0
Pro	3.592ProAla: 3.592 ± 0.027	1.331ProCys: 1.331 ± 0.032	3.199ProAsp: 3.199 ± 0.028	3.276ProGlu: 3.276 ± 0.024	1.709ProPhe: 1.709 ± 0.012	4.018ProGly: 4.018 ± 0.037	1.242ProHis: 1.242 ± 0.011	2.315ProIle: 2.315 ± 0.013	2.54ProLys: 2.54 ± 0.022	3.983ProLeu: 3.983 ± 0.021	1.125ProMet: 1.125 ± 0.01	2.171ProAsn: 2.171 ± 0.015	4.601ProPro: 4.601 ± 0.047	2.321ProGln: 2.321 ± 0.017	2.52ProArg: 2.52 ± 0.017	5.512ProSer: 5.512 ± 0.037	3.935ProThr: 3.935 ± 0.037	3.67ProVal: 3.67 ± 0.023	0.458ProTrp: 0.458 ± 0.006	1.313ProTyr: 1.313 ± 0.01	0.001ProXaa: 0.001 ± 0.0
Gln	2.85GlnAla: 2.85 ± 0.018	1.043GlnCys: 1.043 ± 0.015	2.467GlnAsp: 2.467 ± 0.013	3.139GlnGlu: 3.139 ± 0.019	1.291GlnPhe: 1.291 ± 0.011	2.878GlnGly: 2.878 ± 0.019	1.144GlnHis: 1.144 ± 0.009	1.883GlnIle: 1.883 ± 0.01	2.323GlnLys: 2.323 ± 0.017	3.627GlnLeu: 3.627 ± 0.02	1.036GlnMet: 1.036 ± 0.009	1.902GlnAsn: 1.902 ± 0.016	2.484GlnPro: 2.484 ± 0.021	3.255GlnGln: 3.255 ± 0.05	2.599GlnArg: 2.599 ± 0.016	3.336GlnSer: 3.336 ± 0.019	2.648GlnThr: 2.648 ± 0.016	2.657GlnVal: 2.657 ± 0.021	0.557GlnTrp: 0.557 ± 0.01	1.172GlnTyr: 1.172 ± 0.009	0.001GlnXaa: 0.001 ± 0.0
Arg	2.881ArgAla: 2.881 ± 0.015	1.109ArgCys: 1.109 ± 0.011	2.989ArgAsp: 2.989 ± 0.017	3.467ArgGlu: 3.467 ± 0.021	1.804ArgPhe: 1.804 ± 0.013	3.203ArgGly: 3.203 ± 0.02	1.435ArgHis: 1.435 ± 0.011	2.437ArgIle: 2.437 ± 0.014	3.454ArgLys: 3.454 ± 0.023	4.624ArgLeu: 4.624 ± 0.023	1.33ArgMet: 1.33 ± 0.01	2.241ArgAsn: 2.241 ± 0.012	2.578ArgPro: 2.578 ± 0.016	2.5ArgGln: 2.5 ± 0.016	3.917ArgArg: 3.917 ± 0.026	4.242ArgSer: 4.242 ± 0.023	3.009ArgThr: 3.009 ± 0.017	2.99ArgVal: 2.99 ± 0.016	0.608ArgTrp: 0.608 ± 0.007	1.551ArgTyr: 1.551 ± 0.01	0.001ArgXaa: 0.001 ± 0.0
Ser	5.129SerAla: 5.129 ± 0.022	1.764SerCys: 1.764 ± 0.019	5.211SerAsp: 5.211 ± 0.026	4.794SerGlu: 4.794 ± 0.026	3.037SerPhe: 3.037 ± 0.018	5.924SerGly: 5.924 ± 0.037	2.04SerHis: 2.04 ± 0.016	3.798SerIle: 3.798 ± 0.018	4.237SerLys: 4.237 ± 0.029	6.852SerLeu: 6.852 ± 0.027	1.943SerMet: 1.943 ± 0.011	3.85SerAsn: 3.85 ± 0.024	5.342SerPro: 5.342 ± 0.036	3.628SerGln: 3.628 ± 0.023	4.321SerArg: 4.321 ± 0.028	10.307SerSer: 10.307 ± 0.051	5.74SerThr: 5.74 ± 0.04	4.959SerVal: 4.959 ± 0.023	0.875SerTrp: 0.875 ± 0.008	2.202SerTyr: 2.202 ± 0.013	0.001SerXaa: 0.001 ± 0.0
Thr	4.352ThrAla: 4.352 ± 0.036	2.217ThrCys: 2.217 ± 0.052	4.021ThrAsp: 4.021 ± 0.041	3.936ThrGlu: 3.936 ± 0.043	2.539ThrPhe: 2.539 ± 0.03	4.247ThrGly: 4.247 ± 0.03	1.318ThrHis: 1.318 ± 0.011	3.205ThrIle: 3.205 ± 0.022	3.028ThrLys: 3.028 ± 0.019	4.952ThrLeu: 4.952 ± 0.022	1.433ThrMet: 1.433 ± 0.01	2.722ThrAsn: 2.722 ± 0.016	4.268ThrPro: 4.268 ± 0.043	2.335ThrGln: 2.335 ± 0.017	2.739ThrArg: 2.739 ± 0.014	6.168ThrSer: 6.168 ± 0.044	5.922ThrThr: 5.922 ± 0.156	4.46ThrVal: 4.46 ± 0.046	0.739ThrTrp: 0.739 ± 0.012	1.668ThrTyr: 1.668 ± 0.017	0.001ThrXaa: 0.001 ± 0.0
Val	4.08ValAla: 4.08 ± 0.017	1.659ValCys: 1.659 ± 0.017	3.896ValAsp: 3.896 ± 0.022	4.038ValGlu: 4.038 ± 0.024	2.427ValPhe: 2.427 ± 0.016	3.586ValGly: 3.586 ± 0.021	1.44ValHis: 1.44 ± 0.01	3.518ValIle: 3.518 ± 0.023	3.378ValLys: 3.378 ± 0.02	5.003ValLeu: 5.003 ± 0.02	1.499ValMet: 1.499 ± 0.01	3.057ValAsn: 3.057 ± 0.027	3.161ValPro: 3.161 ± 0.019	2.669ValGln: 2.669 ± 0.015	3.006ValArg: 3.006 ± 0.015	4.946ValSer: 4.946 ± 0.026	4.862ValThr: 4.862 ± 0.056	4.488ValVal: 4.488 ± 0.025	0.623ValTrp: 0.623 ± 0.006	1.71ValTyr: 1.71 ± 0.011	0.001ValXaa: 0.001 ± 0.0
Trp	0.512TrpAla: 0.512 ± 0.006	0.204TrpCys: 0.204 ± 0.003	0.66TrpAsp: 0.66 ± 0.008	0.584TrpGlu: 0.584 ± 0.006	0.415TrpPhe: 0.415 ± 0.006	0.622TrpGly: 0.622 ± 0.011	0.242TrpHis: 0.242 ± 0.003	0.611TrpIle: 0.611 ± 0.009	0.651TrpLys: 0.651 ± 0.006	0.903TrpLeu: 0.903 ± 0.008	0.328TrpMet: 0.328 ± 0.004	0.522TrpAsn: 0.522 ± 0.006	0.392TrpPro: 0.392 ± 0.005	0.432TrpGln: 0.432 ± 0.005	0.619TrpArg: 0.619 ± 0.006	0.868TrpSer: 0.868 ± 0.01	0.793TrpThr: 0.793 ± 0.012	0.625TrpVal: 0.625 ± 0.007	0.162TrpTrp: 0.162 ± 0.003	0.329TrpTyr: 0.329 ± 0.005	0.0TrpXaa: 0.0 ± 0.0
Tyr	1.59TyrAla: 1.59 ± 0.011	0.708TyrCys: 0.708 ± 0.015	1.668TyrAsp: 1.668 ± 0.012	1.619TyrGlu: 1.619 ± 0.01	1.096TyrPhe: 1.096 ± 0.009	1.782TyrGly: 1.782 ± 0.011	0.783TyrHis: 0.783 ± 0.008	1.544TyrIle: 1.544 ± 0.011	1.293TyrLys: 1.293 ± 0.01	2.42TyrLeu: 2.42 ± 0.017	0.656TyrMet: 0.656 ± 0.006	1.3TyrAsn: 1.3 ± 0.009	1.307TyrPro: 1.307 ± 0.013	1.221TyrGln: 1.221 ± 0.01	1.535TyrArg: 1.535 ± 0.01	2.151TyrSer: 2.151 ± 0.014	2.002TyrThr: 2.002 ± 0.027	1.691TyrVal: 1.691 ± 0.011	0.372TyrTrp: 0.372 ± 0.008	0.96TyrTyr: 0.96 ± 0.012	0.001TyrXaa: 0.001 ± 0.0
Xaa	0.001XaaAla: 0.001 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.001XaaAsp: 0.001 ± 0.0	0.002XaaGlu: 0.002 ± 0.0	0.001XaaPhe: 0.001 ± 0.0	0.002XaaGly: 0.002 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.001XaaIle: 0.001 ± 0.0	0.001XaaLys: 0.001 ± 0.0	0.001XaaLeu: 0.001 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.001XaaAsn: 0.001 ± 0.0	0.002XaaPro: 0.002 ± 0.0	0.001XaaGln: 0.001 ± 0.0	0.001XaaArg: 0.001 ± 0.0	0.001XaaSer: 0.001 ± 0.0	0.001XaaThr: 0.001 ± 0.0	0.001XaaVal: 0.001 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.001XaaTyr: 0.001 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 34423 proteins (23911872 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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