Altererythrobacter sp. BO-6
Average proteome isoelectric point is 6.02
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2774 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A6G6X880|A0A6G6X880_9SPHN ABM domain-containing protein OS=Altererythrobacter sp. BO-6 OX=2604537 GN=G6N82_05930 PE=4 SV=1
MM1 pKa = 8.03 KK2 pKa = 10.1 IRR4 pKa = 11.84 DD5 pKa = 3.63 FAIVSVAAACLISCGNEE22 pKa = 3.64 VAEE25 pKa = 4.72 VATEE29 pKa = 3.83 KK30 pKa = 11.05 DD31 pKa = 3.39 GAEE34 pKa = 4.08 NEE36 pKa = 4.12 AATANTIQTTAAMEE50 pKa = 4.64 AGSGVYY56 pKa = 10.53 GLDD59 pKa = 3.68 CEE61 pKa = 5.12 AEE63 pKa = 4.02 TGRR66 pKa = 11.84 YY67 pKa = 8.97 EE68 pKa = 4.39 IILNKK73 pKa = 10.35 SEE75 pKa = 4.72 DD76 pKa = 4.06 GFVSAQVTDD85 pKa = 3.59 DD86 pKa = 3.35 SGRR89 pKa = 11.84 FEE91 pKa = 5.82 DD92 pKa = 5.5 LLTSYY97 pKa = 11.01 SYY99 pKa = 10.67 MGDD102 pKa = 3.21 ATPADD107 pKa = 4.01 FLIAIMFDD115 pKa = 3.4 PTNAPVPDD123 pKa = 4.39 AEE125 pKa = 4.89 DD126 pKa = 3.45 PRR128 pKa = 11.84 IEE130 pKa = 3.65 IWKK133 pKa = 10.28 GEE135 pKa = 3.8 NAYY138 pKa = 10.68 YY139 pKa = 11.1 GLVNGNRR146 pKa = 11.84 DD147 pKa = 3.18 EE148 pKa = 4.62 RR149 pKa = 11.84 LDD151 pKa = 3.53 YY152 pKa = 11.17 CADD155 pKa = 3.26 VSGG158 pKa = 4.13
Molecular weight: 16.92 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.75
IPC2_protein 3.846
IPC_protein 3.821
Toseland 3.617
ProMoST 3.973
Dawson 3.795
Bjellqvist 3.948
Wikipedia 3.706
Rodwell 3.643
Grimsley 3.528
Solomon 3.783
Lehninger 3.745
Nozaki 3.91
DTASelect 4.101
Thurlkill 3.656
EMBOSS 3.719
Sillero 3.935
Patrickios 1.875
IPC_peptide 3.783
IPC2_peptide 3.91
IPC2.peptide.svr19 3.837
Protein with the highest isoelectric point:
>tr|A0A6G6XD35|A0A6G6XD35_9SPHN S1/P1 nuclease OS=Altererythrobacter sp. BO-6 OX=2604537 GN=G6N82_06700 PE=4 SV=1
MM1 pKa = 7.45 KK2 pKa = 9.61 RR3 pKa = 11.84 TFQPSNLVRR12 pKa = 11.84 ARR14 pKa = 11.84 RR15 pKa = 11.84 HH16 pKa = 4.89 GFFARR21 pKa = 11.84 KK22 pKa = 7.42 ATPGGRR28 pKa = 11.84 KK29 pKa = 8.06 VLRR32 pKa = 11.84 ARR34 pKa = 11.84 RR35 pKa = 11.84 KK36 pKa = 8.8 RR37 pKa = 11.84 GRR39 pKa = 11.84 KK40 pKa = 8.46 NLCAA44 pKa = 4.54
Molecular weight: 5.15 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.423
IPC2_protein 10.906
IPC_protein 12.501
Toseland 12.661
ProMoST 13.159
Dawson 12.661
Bjellqvist 12.661
Wikipedia 13.13
Rodwell 12.34
Grimsley 12.705
Solomon 13.159
Lehninger 13.056
Nozaki 12.661
DTASelect 12.661
Thurlkill 12.661
EMBOSS 13.159
Sillero 12.661
Patrickios 12.076
IPC_peptide 13.159
IPC2_peptide 12.149
IPC2.peptide.svr19 9.092
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2774
0
2774
866373
38
3547
312.3
33.92
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
12.578 ± 0.064
0.847 ± 0.015
5.951 ± 0.044
6.291 ± 0.048
3.761 ± 0.032
8.85 ± 0.042
2.005 ± 0.023
5.147 ± 0.034
3.279 ± 0.039
9.807 ± 0.053
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.519 ± 0.026
2.703 ± 0.036
5.157 ± 0.034
3.277 ± 0.025
6.805 ± 0.05
5.286 ± 0.034
5.022 ± 0.051
6.933 ± 0.037
1.475 ± 0.02
2.307 ± 0.024
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here