Megasphaera elsdenii DSM 20460

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Negativicutes; Veillonellales; Veillonellaceae; Megasphaera; Megasphaera elsdenii

Average proteome isoelectric point is 6.31

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2194 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|G0VRB4|G0VRB4_MEGEL Type III pantothenate kinase OS=Megasphaera elsdenii DSM 20460 OX=1064535 GN=coaX PE=3 SV=1
MM1 pKa = 7.66EE2 pKa = 4.52STVFTNLRR10 pKa = 11.84GSEE13 pKa = 4.09GALTFNFFCEE23 pKa = 4.09SLITSLHH30 pKa = 5.68TLTHH34 pKa = 6.0VMEE37 pKa = 5.27DD38 pKa = 3.04AGIAVPDD45 pKa = 3.89NVGDD49 pKa = 3.75VADD52 pKa = 4.65ALGEE56 pKa = 4.19MGSHH60 pKa = 6.78LMEE63 pKa = 5.49DD64 pKa = 4.05YY65 pKa = 10.94QRR67 pKa = 11.84GEE69 pKa = 3.87LDD71 pKa = 3.06LGRR74 pKa = 11.84FKK76 pKa = 11.44DD77 pKa = 4.26EE78 pKa = 4.38ILDD81 pKa = 4.17FYY83 pKa = 11.66DD84 pKa = 5.22LNFAVNDD91 pKa = 4.2ALASAIMSHH100 pKa = 7.82DD101 pKa = 3.74DD102 pKa = 3.34LQYY105 pKa = 9.89YY106 pKa = 8.81YY107 pKa = 10.55YY108 pKa = 11.29VYY110 pKa = 10.02MQGLYY115 pKa = 10.19IFFPNMMEE123 pKa = 4.5AFNADD128 pKa = 3.29IEE130 pKa = 4.4DD131 pKa = 3.69EE132 pKa = 4.46KK133 pKa = 10.97IIPFLDD139 pKa = 3.18EE140 pKa = 4.0LANEE144 pKa = 4.52FRR146 pKa = 11.84QLAGSGSS153 pKa = 3.45

Molecular weight: 17.26 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|G0VQG8|G0VQG8_MEGEL Predicted transcriptional regulator OS=Megasphaera elsdenii DSM 20460 OX=1064535 GN=MELS_0001 PE=3 SV=1
MM1 pKa = 6.87STQRR5 pKa = 11.84GQKK8 pKa = 9.38PRR10 pKa = 11.84RR11 pKa = 11.84QVTMTPAVLTRR22 pKa = 11.84IIAIVLLIGIVAFLMIHH39 pKa = 7.07KK40 pKa = 9.94II41 pKa = 3.59

Molecular weight: 4.62 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2194

0

2194

702803

31

2698

320.3

35.51

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.224 ± 0.063

1.391 ± 0.022

6.11 ± 0.042

5.942 ± 0.046

3.884 ± 0.037

7.619 ± 0.054

2.335 ± 0.027

6.598 ± 0.047

5.899 ± 0.045

9.139 ± 0.056

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.954 ± 0.026

3.55 ± 0.051

3.976 ± 0.035

3.764 ± 0.038

4.908 ± 0.044

5.256 ± 0.041

5.547 ± 0.048

7.25 ± 0.043

1.007 ± 0.019

3.65 ± 0.03
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski