Amino acid dipepetide frequency for Megasphaera elsdenii DSM 20460

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
10.721AlaAla: 10.721 ± 0.196
1.399AlaCys: 1.399 ± 0.048
5.835AlaAsp: 5.835 ± 0.098
5.033AlaGlu: 5.033 ± 0.088
3.577AlaPhe: 3.577 ± 0.085
7.328AlaGly: 7.328 ± 0.146
1.808AlaHis: 1.808 ± 0.055
6.125AlaIle: 6.125 ± 0.097
5.347AlaLys: 5.347 ± 0.104
8.773AlaLeu: 8.773 ± 0.136
3.055AlaMet: 3.055 ± 0.075
2.775AlaAsn: 2.775 ± 0.075
2.88AlaPro: 2.88 ± 0.072
3.318AlaGln: 3.318 ± 0.079
3.876AlaArg: 3.876 ± 0.094
4.604AlaSer: 4.604 ± 0.119
3.869AlaThr: 3.869 ± 0.117
7.874AlaVal: 7.874 ± 0.145
0.956AlaTrp: 0.956 ± 0.039
3.082AlaTyr: 3.082 ± 0.069
0.0AlaXaa: 0.0 ± 0.0
Cys
0.956CysAla: 0.956 ± 0.044
0.289CysCys: 0.289 ± 0.024
0.805CysAsp: 0.805 ± 0.038
0.615CysGlu: 0.615 ± 0.033
0.515CysPhe: 0.515 ± 0.03
1.43CysGly: 1.43 ± 0.049
0.457CysHis: 0.457 ± 0.026
0.904CysIle: 0.904 ± 0.036
0.538CysLys: 0.538 ± 0.026
1.346CysLeu: 1.346 ± 0.044
0.371CysMet: 0.371 ± 0.025
0.415CysAsn: 0.415 ± 0.026
0.781CysPro: 0.781 ± 0.037
0.69CysGln: 0.69 ± 0.03
0.891CysArg: 0.891 ± 0.038
0.716CysSer: 0.716 ± 0.036
0.726CysThr: 0.726 ± 0.029
0.854CysVal: 0.854 ± 0.04
0.12CysTrp: 0.12 ± 0.014
0.488CysTyr: 0.488 ± 0.025
0.0CysXaa: 0.0 ± 0.0
Asp
5.115AspAla: 5.115 ± 0.104
0.8AspCys: 0.8 ± 0.036
3.886AspAsp: 3.886 ± 0.094
4.306AspGlu: 4.306 ± 0.093
2.548AspPhe: 2.548 ± 0.068
5.007AspGly: 5.007 ± 0.122
1.283AspHis: 1.283 ± 0.041
4.387AspIle: 4.387 ± 0.083
3.647AspLys: 3.647 ± 0.08
5.369AspLeu: 5.369 ± 0.086
1.948AspMet: 1.948 ± 0.049
2.022AspAsn: 2.022 ± 0.059
2.099AspPro: 2.099 ± 0.059
1.729AspGln: 1.729 ± 0.058
2.627AspArg: 2.627 ± 0.057
2.807AspSer: 2.807 ± 0.064
3.256AspThr: 3.256 ± 0.071
4.927AspVal: 4.927 ± 0.087
0.706AspTrp: 0.706 ± 0.032
2.628AspTyr: 2.628 ± 0.066
0.0AspXaa: 0.0 ± 0.0
Glu
5.858GluAla: 5.858 ± 0.103
0.565GluCys: 0.565 ± 0.031
3.532GluAsp: 3.532 ± 0.087
4.427GluGlu: 4.427 ± 0.095
1.808GluPhe: 1.808 ± 0.055
4.16GluGly: 4.16 ± 0.094
1.264GluHis: 1.264 ± 0.047
3.698GluIle: 3.698 ± 0.072
5.063GluLys: 5.063 ± 0.092
5.26GluLeu: 5.26 ± 0.1
1.834GluMet: 1.834 ± 0.05
2.6GluAsn: 2.6 ± 0.067
1.853GluPro: 1.853 ± 0.057
2.413GluGln: 2.413 ± 0.071
3.065GluArg: 3.065 ± 0.078
2.56GluSer: 2.56 ± 0.061
3.182GluThr: 3.182 ± 0.08
3.722GluVal: 3.722 ± 0.085
0.588GluTrp: 0.588 ± 0.029
1.966GluTyr: 1.966 ± 0.058
0.0GluXaa: 0.0 ± 0.0
Phe
3.344PheAla: 3.344 ± 0.068
0.656PheCys: 0.656 ± 0.035
2.445PheAsp: 2.445 ± 0.07
1.757PheGlu: 1.757 ± 0.052
1.89PhePhe: 1.89 ± 0.07
3.12PheGly: 3.12 ± 0.07
0.996PheHis: 0.996 ± 0.039
2.676PheIle: 2.676 ± 0.079
1.678PheLys: 1.678 ± 0.052
3.709PheLeu: 3.709 ± 0.106
1.071PheMet: 1.071 ± 0.037
1.328PheAsn: 1.328 ± 0.054
1.504PhePro: 1.504 ± 0.049
1.345PheGln: 1.345 ± 0.048
1.837PheArg: 1.837 ± 0.058
2.503PheSer: 2.503 ± 0.059
2.442PheThr: 2.442 ± 0.063
2.787PheVal: 2.787 ± 0.074
0.391PheTrp: 0.391 ± 0.023
1.36PheTyr: 1.36 ± 0.044
0.0PheXaa: 0.0 ± 0.0
Gly
6.219GlyAla: 6.219 ± 0.122
1.248GlyCys: 1.248 ± 0.045
4.183GlyAsp: 4.183 ± 0.084
3.759GlyGlu: 3.759 ± 0.069
3.049GlyPhe: 3.049 ± 0.066
5.569GlyGly: 5.569 ± 0.114
1.911GlyHis: 1.911 ± 0.056
5.643GlyIle: 5.643 ± 0.095
5.25GlyLys: 5.25 ± 0.088
7.119GlyLeu: 7.119 ± 0.123
2.439GlyMet: 2.439 ± 0.055
2.793GlyAsn: 2.793 ± 0.085
2.103GlyPro: 2.103 ± 0.069
2.908GlyGln: 2.908 ± 0.073
3.919GlyArg: 3.919 ± 0.083
4.262GlySer: 4.262 ± 0.101
4.814GlyThr: 4.814 ± 0.112
5.287GlyVal: 5.287 ± 0.097
0.757GlyTrp: 0.757 ± 0.032
2.955GlyTyr: 2.955 ± 0.078
0.0GlyXaa: 0.0 ± 0.0
His
1.494HisAla: 1.494 ± 0.052
0.437HisCys: 0.437 ± 0.029
1.456HisAsp: 1.456 ± 0.043
1.396HisGlu: 1.396 ± 0.045
1.131HisPhe: 1.131 ± 0.041
1.844HisGly: 1.844 ± 0.058
0.74HisHis: 0.74 ± 0.036
1.773HisIle: 1.773 ± 0.051
1.14HisLys: 1.14 ± 0.043
2.082HisLeu: 2.082 ± 0.054
0.72HisMet: 0.72 ± 0.031
0.785HisAsn: 0.785 ± 0.03
1.204HisPro: 1.204 ± 0.039
0.768HisGln: 0.768 ± 0.033
1.147HisArg: 1.147 ± 0.042
1.134HisSer: 1.134 ± 0.038
1.239HisThr: 1.239 ± 0.045
1.655HisVal: 1.655 ± 0.052
0.297HisTrp: 0.297 ± 0.02
0.905HisTyr: 0.905 ± 0.042
0.0HisXaa: 0.0 ± 0.0
Ile
6.295IleAla: 6.295 ± 0.106
1.054IleCys: 1.054 ± 0.042
4.205IleAsp: 4.205 ± 0.094
3.564IleGlu: 3.564 ± 0.083
2.496IlePhe: 2.496 ± 0.08
5.265IleGly: 5.265 ± 0.097
1.629IleHis: 1.629 ± 0.05
4.34IleIle: 4.34 ± 0.107
3.143IleLys: 3.143 ± 0.066
6.073IleLeu: 6.073 ± 0.119
1.734IleMet: 1.734 ± 0.058
2.285IleAsn: 2.285 ± 0.065
3.126IlePro: 3.126 ± 0.073
2.103IleGln: 2.103 ± 0.061
3.307IleArg: 3.307 ± 0.077
3.93IleSer: 3.93 ± 0.085
3.782IleThr: 3.782 ± 0.09
4.993IleVal: 4.993 ± 0.094
0.558IleTrp: 0.558 ± 0.029
2.099IleTyr: 2.099 ± 0.066
0.0IleXaa: 0.0 ± 0.0
Lys
5.943LysAla: 5.943 ± 0.123
0.541LysCys: 0.541 ± 0.028
3.89LysAsp: 3.89 ± 0.086
4.698LysGlu: 4.698 ± 0.084
1.497LysPhe: 1.497 ± 0.049
4.189LysGly: 4.189 ± 0.079
1.144LysHis: 1.144 ± 0.04
3.61LysIle: 3.61 ± 0.076
4.708LysLys: 4.708 ± 0.095
4.603LysLeu: 4.603 ± 0.077
2.006LysMet: 2.006 ± 0.053
2.398LysAsn: 2.398 ± 0.074
2.043LysPro: 2.043 ± 0.063
2.205LysGln: 2.205 ± 0.059
2.824LysArg: 2.824 ± 0.061
2.638LysSer: 2.638 ± 0.061
3.322LysThr: 3.322 ± 0.076
3.985LysVal: 3.985 ± 0.076
0.517LysTrp: 0.517 ± 0.024
2.23LysTyr: 2.23 ± 0.057
0.0LysXaa: 0.0 ± 0.0
Leu
9.33LeuAla: 9.33 ± 0.143
1.38LeuCys: 1.38 ± 0.049
5.34LeuAsp: 5.34 ± 0.102
4.98LeuGlu: 4.98 ± 0.084
3.607LeuPhe: 3.607 ± 0.091
6.638LeuGly: 6.638 ± 0.106
2.122LeuHis: 2.122 ± 0.059
5.269LeuIle: 5.269 ± 0.109
5.211LeuLys: 5.211 ± 0.097
8.062LeuLeu: 8.062 ± 0.162
2.513LeuMet: 2.513 ± 0.066
3.082LeuAsn: 3.082 ± 0.07
4.469LeuPro: 4.469 ± 0.084
3.547LeuGln: 3.547 ± 0.082
4.614LeuArg: 4.614 ± 0.103
5.643LeuSer: 5.643 ± 0.102
5.637LeuThr: 5.637 ± 0.102
6.019LeuVal: 6.019 ± 0.107
0.844LeuTrp: 0.844 ± 0.036
3.082LeuTyr: 3.082 ± 0.068
0.0LeuXaa: 0.0 ± 0.0
Met
3.216MetAla: 3.216 ± 0.066
0.29MetCys: 0.29 ± 0.019
1.917MetAsp: 1.917 ± 0.05
2.025MetGlu: 2.025 ± 0.06
0.82MetPhe: 0.82 ± 0.038
2.15MetGly: 2.15 ± 0.066
0.518MetHis: 0.518 ± 0.025
1.723MetIle: 1.723 ± 0.059
2.311MetLys: 2.311 ± 0.055
2.271MetLeu: 2.271 ± 0.058
0.959MetMet: 0.959 ± 0.04
1.35MetAsn: 1.35 ± 0.042
1.309MetPro: 1.309 ± 0.043
0.943MetGln: 0.943 ± 0.031
1.258MetArg: 1.258 ± 0.045
1.598MetSer: 1.598 ± 0.046
2.043MetThr: 2.043 ± 0.052
1.769MetVal: 1.769 ± 0.055
0.189MetTrp: 0.189 ± 0.016
0.886MetTyr: 0.886 ± 0.036
0.0MetXaa: 0.0 ± 0.0
Asn
2.732AsnAla: 2.732 ± 0.076
0.47AsnCys: 0.47 ± 0.023
1.982AsnAsp: 1.982 ± 0.055
1.956AsnGlu: 1.956 ± 0.061
1.266AsnPhe: 1.266 ± 0.043
2.994AsnGly: 2.994 ± 0.099
0.876AsnHis: 0.876 ± 0.039
2.608AsnIle: 2.608 ± 0.067
1.983AsnLys: 1.983 ± 0.07
3.288AsnLeu: 3.288 ± 0.079
1.11AsnMet: 1.11 ± 0.041
1.31AsnAsn: 1.31 ± 0.061
1.925AsnPro: 1.925 ± 0.057
1.241AsnGln: 1.241 ± 0.044
1.81AsnArg: 1.81 ± 0.058
1.71AsnSer: 1.71 ± 0.07
1.927AsnThr: 1.927 ± 0.075
2.634AsnVal: 2.634 ± 0.07
0.398AsnTrp: 0.398 ± 0.024
1.278AsnTyr: 1.278 ± 0.043
0.0AsnXaa: 0.0 ± 0.0
Pro
3.707ProAla: 3.707 ± 0.087
0.474ProCys: 0.474 ± 0.024
2.992ProAsp: 2.992 ± 0.066
3.143ProGlu: 3.143 ± 0.086
1.803ProPhe: 1.803 ± 0.057
2.837ProGly: 2.837 ± 0.071
0.857ProHis: 0.857 ± 0.035
2.41ProIle: 2.41 ± 0.057
2.089ProLys: 2.089 ± 0.06
3.489ProLeu: 3.489 ± 0.083
1.09ProMet: 1.09 ± 0.038
1.229ProAsn: 1.229 ± 0.047
1.097ProPro: 1.097 ± 0.044
1.589ProGln: 1.589 ± 0.049
1.414ProArg: 1.414 ± 0.05
2.012ProSer: 2.012 ± 0.054
1.894ProThr: 1.894 ± 0.051
3.547ProVal: 3.547 ± 0.08
0.424ProTrp: 0.424 ± 0.024
1.665ProTyr: 1.665 ± 0.053
0.0ProXaa: 0.0 ± 0.0
Gln
4.299GlnAla: 4.299 ± 0.105
0.414GlnCys: 0.414 ± 0.024
1.964GlnAsp: 1.964 ± 0.051
2.469GlnGlu: 2.469 ± 0.066
1.373GlnPhe: 1.373 ± 0.046
2.758GlnGly: 2.758 ± 0.062
0.956GlnHis: 0.956 ± 0.033
2.137GlnIle: 2.137 ± 0.05
2.382GlnLys: 2.382 ± 0.068
3.15GlnLeu: 3.15 ± 0.073
1.106GlnMet: 1.106 ± 0.04
1.288GlnAsn: 1.288 ± 0.051
1.461GlnPro: 1.461 ± 0.057
1.834GlnGln: 1.834 ± 0.06
2.06GlnArg: 2.06 ± 0.064
1.733GlnSer: 1.733 ± 0.049
1.944GlnThr: 1.944 ± 0.062
2.4GlnVal: 2.4 ± 0.057
0.413GlnTrp: 0.413 ± 0.029
1.501GlnTyr: 1.501 ± 0.046
0.0GlnXaa: 0.0 ± 0.0
Arg
3.408ArgAla: 3.408 ± 0.072
0.692ArgCys: 0.692 ± 0.03
2.886ArgAsp: 2.886 ± 0.078
2.998ArgGlu: 2.998 ± 0.072
1.961ArgPhe: 1.961 ± 0.054
2.904ArgGly: 2.904 ± 0.065
1.423ArgHis: 1.423 ± 0.054
3.487ArgIle: 3.487 ± 0.069
2.961ArgLys: 2.961 ± 0.069
4.742ArgLeu: 4.742 ± 0.091
1.322ArgMet: 1.322 ± 0.04
1.826ArgAsn: 1.826 ± 0.05
2.018ArgPro: 2.018 ± 0.061
2.659ArgGln: 2.659 ± 0.073
3.413ArgArg: 3.413 ± 0.085
2.392ArgSer: 2.392 ± 0.056
2.484ArgThr: 2.484 ± 0.055
3.025ArgVal: 3.025 ± 0.075
0.546ArgTrp: 0.546 ± 0.028
1.929ArgTyr: 1.929 ± 0.058
0.0ArgXaa: 0.0 ± 0.0
Ser
4.27SerAla: 4.27 ± 0.104
0.674SerCys: 0.674 ± 0.035
2.955SerAsp: 2.955 ± 0.059
2.655SerGlu: 2.655 ± 0.065
2.328SerPhe: 2.328 ± 0.057
4.408SerGly: 4.408 ± 0.09
1.47SerHis: 1.47 ± 0.047
3.396SerIle: 3.396 ± 0.074
2.511SerLys: 2.511 ± 0.069
5.578SerLeu: 5.578 ± 0.111
1.508SerMet: 1.508 ± 0.051
1.723SerAsn: 1.723 ± 0.076
2.096SerPro: 2.096 ± 0.059
2.309SerGln: 2.309 ± 0.064
2.787SerArg: 2.787 ± 0.067
2.85SerSer: 2.85 ± 0.075
2.659SerThr: 2.659 ± 0.078
3.702SerVal: 3.702 ± 0.081
0.67SerTrp: 0.67 ± 0.035
2.005SerTyr: 2.005 ± 0.057
0.0SerXaa: 0.0 ± 0.0
Thr
5.507ThrAla: 5.507 ± 0.105
0.724ThrCys: 0.724 ± 0.035
3.216ThrAsp: 3.216 ± 0.073
2.982ThrGlu: 2.982 ± 0.07
2.195ThrPhe: 2.195 ± 0.06
4.937ThrGly: 4.937 ± 0.105
1.077ThrHis: 1.077 ± 0.039
3.835ThrIle: 3.835 ± 0.085
2.833ThrLys: 2.833 ± 0.065
5.238ThrLeu: 5.238 ± 0.085
1.473ThrMet: 1.473 ± 0.045
1.904ThrAsn: 1.904 ± 0.093
2.651ThrPro: 2.651 ± 0.068
1.551ThrGln: 1.551 ± 0.048
2.056ThrArg: 2.056 ± 0.05
2.708ThrSer: 2.708 ± 0.079
3.211ThrThr: 3.211 ± 0.093
4.717ThrVal: 4.717 ± 0.119
0.542ThrTrp: 0.542 ± 0.03
2.109ThrTyr: 2.109 ± 0.062
0.0ThrXaa: 0.0 ± 0.0
Val
6.202ValAla: 6.202 ± 0.113
1.141ValCys: 1.141 ± 0.044
4.283ValAsp: 4.283 ± 0.087
3.984ValGlu: 3.984 ± 0.087
2.948ValPhe: 2.948 ± 0.068
4.814ValGly: 4.814 ± 0.083
1.591ValHis: 1.591 ± 0.045
5.007ValIle: 5.007 ± 0.096
3.785ValLys: 3.785 ± 0.083
7.026ValLeu: 7.026 ± 0.125
2.033ValMet: 2.033 ± 0.055
2.543ValAsn: 2.543 ± 0.065
3.337ValPro: 3.337 ± 0.071
2.481ValGln: 2.481 ± 0.057
3.702ValArg: 3.702 ± 0.072
4.35ValSer: 4.35 ± 0.075
4.59ValThr: 4.59 ± 0.093
5.504ValVal: 5.504 ± 0.107
0.646ValTrp: 0.646 ± 0.034
2.528ValTyr: 2.528 ± 0.065
0.0ValXaa: 0.0 ± 0.0
Trp
0.686TrpAla: 0.686 ± 0.03
0.128TrpCys: 0.128 ± 0.012
0.639TrpAsp: 0.639 ± 0.032
0.498TrpGlu: 0.498 ± 0.028
0.434TrpPhe: 0.434 ± 0.026
0.679TrpGly: 0.679 ± 0.028
0.303TrpHis: 0.303 ± 0.02
0.613TrpIle: 0.613 ± 0.033
0.642TrpLys: 0.642 ± 0.031
1.033TrpLeu: 1.033 ± 0.049
0.31TrpMet: 0.31 ± 0.025
0.467TrpAsn: 0.467 ± 0.028
0.349TrpPro: 0.349 ± 0.022
0.69TrpGln: 0.69 ± 0.032
0.544TrpArg: 0.544 ± 0.027
0.484TrpSer: 0.484 ± 0.025
0.474TrpThr: 0.474 ± 0.026
0.562TrpVal: 0.562 ± 0.027
0.138TrpTrp: 0.138 ± 0.013
0.396TrpTyr: 0.396 ± 0.029
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.935TyrAla: 2.935 ± 0.064
0.531TyrCys: 0.531 ± 0.027
2.686TyrAsp: 2.686 ± 0.065
2.171TyrGlu: 2.171 ± 0.063
1.592TyrPhe: 1.592 ± 0.052
3.157TyrGly: 3.157 ± 0.08
0.922TyrHis: 0.922 ± 0.041
2.338TyrIle: 2.338 ± 0.059
1.771TyrLys: 1.771 ± 0.051
3.177TyrLeu: 3.177 ± 0.074
0.941TyrMet: 0.941 ± 0.038
1.377TyrAsn: 1.377 ± 0.048
1.458TyrPro: 1.458 ± 0.05
1.316TyrGln: 1.316 ± 0.046
1.925TyrArg: 1.925 ± 0.064
1.924TyrSer: 1.924 ± 0.06
1.971TyrThr: 1.971 ± 0.062
2.531TyrVal: 2.531 ± 0.064
0.367TyrTrp: 0.367 ± 0.026
1.411TyrTyr: 1.411 ± 0.053
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 2194 proteins (702803 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski