Pararhizobium polonicum

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales; Rhizobiaceae; Rhizobium/Agrobacterium group; Pararhizobium

Average proteome isoelectric point is 6.42

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5658 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1C7P209|A0A1C7P209_9RHIZ L-threonine aldolase OS=Pararhizobium polonicum OX=1612624 GN=ADU59_12060 PE=3 SV=1
MM1 pKa = 7.89RR2 pKa = 11.84KK3 pKa = 7.48TVLNALGEE11 pKa = 4.35TLFYY15 pKa = 10.75SDD17 pKa = 3.94SSSGFFSATGSGPTLYY33 pKa = 9.14GTAGNDD39 pKa = 4.03SMWGDD44 pKa = 3.58SAVNVTMSGGTGDD57 pKa = 4.91DD58 pKa = 2.72IYY60 pKa = 11.64YY61 pKa = 10.28LYY63 pKa = 10.97SAINRR68 pKa = 11.84AYY70 pKa = 8.75EE71 pKa = 3.86AAGEE75 pKa = 4.54GIDD78 pKa = 5.25TISTWMSYY86 pKa = 7.29TLPDD90 pKa = 3.39NFEE93 pKa = 4.16NLIVTGDD100 pKa = 3.47LRR102 pKa = 11.84HH103 pKa = 6.38AFGNSADD110 pKa = 4.01NIITGGTGRR119 pKa = 11.84QTIDD123 pKa = 2.81GGAGNDD129 pKa = 3.45VLTGGGGADD138 pKa = 3.05TFIFSKK144 pKa = 11.18GNGSDD149 pKa = 4.93LITDD153 pKa = 4.45FAADD157 pKa = 3.4DD158 pKa = 3.97TVRR161 pKa = 11.84LNGYY165 pKa = 9.12SLTSFDD171 pKa = 4.0QLLSNTTQEE180 pKa = 4.4GANLRR185 pKa = 11.84LDD187 pKa = 4.09LGDD190 pKa = 4.17GEE192 pKa = 4.98SLVFADD198 pKa = 4.55TTASDD203 pKa = 3.79LNAGQFEE210 pKa = 4.57LSLDD214 pKa = 3.7RR215 pKa = 11.84SALTQTFNDD224 pKa = 3.58NFDD227 pKa = 3.79TLSLVNGTSGVWEE240 pKa = 4.25AKK242 pKa = 10.05YY243 pKa = 9.18WWAPDD248 pKa = 3.07KK249 pKa = 11.31GATLTEE255 pKa = 4.14NGEE258 pKa = 4.27LQWYY262 pKa = 7.8VNPLYY267 pKa = 10.95APTSAVNPFSIDD279 pKa = 3.35NGVLTITAEE288 pKa = 3.92QTPASISAEE297 pKa = 4.05VEE299 pKa = 4.33GYY301 pKa = 10.77DD302 pKa = 3.71YY303 pKa = 11.14TSGMLTTHH311 pKa = 7.26ASFAQTYY318 pKa = 9.42GYY320 pKa = 10.77FEE322 pKa = 4.72IRR324 pKa = 11.84ADD326 pKa = 3.63MPDD329 pKa = 4.41DD330 pKa = 4.11KK331 pKa = 11.4GVWPAFWLLPEE342 pKa = 5.2DD343 pKa = 4.82GSWPPEE349 pKa = 3.78LDD351 pKa = 3.52VVEE354 pKa = 5.06MRR356 pKa = 11.84GQDD359 pKa = 3.55PNTVHH364 pKa = 5.48VTVHH368 pKa = 5.78SNEE371 pKa = 4.45TGSQTSVSSAVSVSDD386 pKa = 3.24TEE388 pKa = 4.82GFHH391 pKa = 6.5TYY393 pKa = 9.81GVLWQEE399 pKa = 5.31DD400 pKa = 4.39EE401 pKa = 4.4IVWYY405 pKa = 9.99IDD407 pKa = 3.26DD408 pKa = 4.0VAVARR413 pKa = 11.84ADD415 pKa = 3.9TPSDD419 pKa = 3.23MHH421 pKa = 7.22EE422 pKa = 4.05PMYY425 pKa = 10.32MLVNLAVGGMAGTPTDD441 pKa = 3.87GLPDD445 pKa = 3.95GSEE448 pKa = 3.71MKK450 pKa = 9.8IDD452 pKa = 3.92YY453 pKa = 10.28IRR455 pKa = 11.84AYY457 pKa = 9.6TLDD460 pKa = 4.0DD461 pKa = 3.86LAA463 pKa = 6.68

Molecular weight: 49.67 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1C7P6G5|A0A1C7P6G5_9RHIZ Nodulation protein N OS=Pararhizobium polonicum OX=1612624 GN=ADU59_11890 PE=4 SV=1
MM1 pKa = 7.62TKK3 pKa = 9.11RR4 pKa = 11.84TYY6 pKa = 10.36QPSKK10 pKa = 9.73LVRR13 pKa = 11.84KK14 pKa = 9.15RR15 pKa = 11.84RR16 pKa = 11.84HH17 pKa = 4.64GFRR20 pKa = 11.84ARR22 pKa = 11.84IATKK26 pKa = 9.98GGRR29 pKa = 11.84KK30 pKa = 9.73VIVARR35 pKa = 11.84RR36 pKa = 11.84ARR38 pKa = 11.84GRR40 pKa = 11.84KK41 pKa = 9.03RR42 pKa = 11.84LSAA45 pKa = 4.03

Molecular weight: 5.28 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5658

0

5658

1784372

29

2875

315.4

34.24

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.91 ± 0.039

0.783 ± 0.011

5.715 ± 0.024

5.521 ± 0.032

3.989 ± 0.02

8.465 ± 0.029

1.995 ± 0.015

5.876 ± 0.025

3.72 ± 0.025

10.026 ± 0.036

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.601 ± 0.015

2.852 ± 0.018

4.846 ± 0.019

3.113 ± 0.019

6.408 ± 0.028

5.763 ± 0.024

5.536 ± 0.02

7.309 ± 0.024

1.255 ± 0.012

2.319 ± 0.016
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski