Streptomyces paucisporeus

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Streptomycetales; Streptomycetaceae; Streptomyces

Average proteome isoelectric point is 6.49

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 7034 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1M6WSL4|A0A1M6WSL4_9ACTN Energy-dependent translational throttle protein EttA OS=Streptomyces paucisporeus OX=310782 GN=ettA PE=3 SV=1
MM1 pKa = 7.35ARR3 pKa = 11.84WDD5 pKa = 3.92AEE7 pKa = 4.15NQRR10 pKa = 11.84WAQDD14 pKa = 3.94PPPGAAGGPGPASVPRR30 pKa = 11.84TPGGNSRR37 pKa = 11.84VLIVALAVVLVCAAAGIGLWAAQTDD62 pKa = 3.92SGGGGGSSPAAGAPGFGDD80 pKa = 3.55GTSAYY85 pKa = 10.61SPDD88 pKa = 3.83PATAGDD94 pKa = 4.41SDD96 pKa = 4.24TTDD99 pKa = 3.37LPTTEE104 pKa = 5.83DD105 pKa = 3.67PTTDD109 pKa = 3.58FPTTEE114 pKa = 4.58DD115 pKa = 4.15PSTEE119 pKa = 4.07PATTGPPPGFAHH131 pKa = 6.32LQDD134 pKa = 3.7VAGFTLDD141 pKa = 4.84VPEE144 pKa = 4.07SWQRR148 pKa = 11.84SSGGASVYY156 pKa = 10.22YY157 pKa = 9.9QSQDD161 pKa = 3.31QQGLIQVFSLSSPQTTPYY179 pKa = 10.49EE180 pKa = 4.19SLKK183 pKa = 10.07ATEE186 pKa = 4.64ATVSTNNGYY195 pKa = 9.19QLLGLRR201 pKa = 11.84YY202 pKa = 9.72IIDD205 pKa = 4.16DD206 pKa = 3.92QASDD210 pKa = 3.79AADD213 pKa = 3.47LEE215 pKa = 4.55YY216 pKa = 10.39TYY218 pKa = 11.42VRR220 pKa = 11.84DD221 pKa = 4.19DD222 pKa = 3.25GGTRR226 pKa = 11.84HH227 pKa = 5.29VVDD230 pKa = 4.5RR231 pKa = 11.84AFTGPDD237 pKa = 3.2GVQYY241 pKa = 11.25ALLVAGPDD249 pKa = 4.09SDD251 pKa = 3.05WSSYY255 pKa = 9.69QDD257 pKa = 3.17VFRR260 pKa = 11.84VLRR263 pKa = 11.84ASFCPDD269 pKa = 3.2NYY271 pKa = 9.73CTQQ274 pKa = 3.17

Molecular weight: 28.49 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1M7CFI9|A0A1M7CFI9_9ACTN Uncharacterized protein OS=Streptomyces paucisporeus OX=310782 GN=SAMN05216499_105224 PE=4 SV=1
MM1 pKa = 7.69SKK3 pKa = 9.0RR4 pKa = 11.84TFQPNNRR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84AKK15 pKa = 8.7THH17 pKa = 5.15GFRR20 pKa = 11.84LRR22 pKa = 11.84MRR24 pKa = 11.84TRR26 pKa = 11.84AGRR29 pKa = 11.84AILATRR35 pKa = 11.84RR36 pKa = 11.84GKK38 pKa = 10.48GRR40 pKa = 11.84ARR42 pKa = 11.84LSAA45 pKa = 3.91

Molecular weight: 5.27 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

7034

0

7034

2374895

27

4814

337.6

35.88

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

14.412 ± 0.038

0.784 ± 0.008

6.032 ± 0.024

4.912 ± 0.034

2.662 ± 0.016

9.744 ± 0.024

2.323 ± 0.014

3.008 ± 0.019

1.862 ± 0.021

10.172 ± 0.037

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.657 ± 0.013

1.801 ± 0.019

6.331 ± 0.026

2.903 ± 0.019

7.867 ± 0.038

5.179 ± 0.027

6.367 ± 0.032

8.329 ± 0.027

1.524 ± 0.012

2.129 ± 0.015
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski