Enhydra lutris papillomavirus 1
Average proteome isoelectric point is 6.4
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 7 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|W8R555|W8R555_9PAPI Major capsid protein L1 OS=Enhydra lutris papillomavirus 1 OX=1472717 GN=L1 PE=3 SV=1
MM1 pKa = 7.62 FGLCPTLGEE10 pKa = 4.4 VVLTEE15 pKa = 3.86 QPEE18 pKa = 4.5 AIDD21 pKa = 3.39 LHH23 pKa = 8.68 CYY25 pKa = 8.29 EE26 pKa = 5.32 HH27 pKa = 6.65 MPSDD31 pKa = 3.89 DD32 pKa = 4.03 EE33 pKa = 4.51 EE34 pKa = 4.99 EE35 pKa = 4.37 EE36 pKa = 4.3 EE37 pKa = 4.17 QTSRR41 pKa = 11.84 DD42 pKa = 3.7 LYY44 pKa = 10.61 RR45 pKa = 11.84 VSVDD49 pKa = 3.09 CGACKK54 pKa = 9.61 RR55 pKa = 11.84 TVTLVVFADD64 pKa = 4.02 LEE66 pKa = 4.92 DD67 pKa = 3.68 IQKK70 pKa = 10.08 LHH72 pKa = 6.64 SLLHH76 pKa = 5.14 SVRR79 pKa = 11.84 VVCEE83 pKa = 3.77 SCLNPNNLNHH93 pKa = 6.88 GGG95 pKa = 3.53
Molecular weight: 10.64 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.243
IPC2_protein 4.507
IPC_protein 4.393
Toseland 4.253
ProMoST 4.507
Dawson 4.342
Bjellqvist 4.495
Wikipedia 4.215
Rodwell 4.24
Grimsley 4.177
Solomon 4.342
Lehninger 4.291
Nozaki 4.469
DTASelect 4.596
Thurlkill 4.266
EMBOSS 4.24
Sillero 4.52
Patrickios 1.074
IPC_peptide 4.355
IPC2_peptide 4.507
IPC2.peptide.svr19 4.42
Protein with the highest isoelectric point:
>tr|W8S105|W8S105_9PAPI Replication protein E1 OS=Enhydra lutris papillomavirus 1 OX=1472717 GN=E1 PE=3 SV=1
MM1 pKa = 7.08 EE2 pKa = 5.13 TLNQALNSVQEE13 pKa = 4.54 DD14 pKa = 4.05 LLSLYY19 pKa = 8.41 EE20 pKa = 4.12 QNSEE24 pKa = 4.76 SITDD28 pKa = 4.7 QIQHH32 pKa = 5.95 WNLLRR37 pKa = 11.84 RR38 pKa = 11.84 EE39 pKa = 4.01 QVILYY44 pKa = 6.84 YY45 pKa = 10.69 ARR47 pKa = 11.84 EE48 pKa = 3.9 RR49 pKa = 11.84 GITRR53 pKa = 11.84 LGMTLVPPRR62 pKa = 11.84 HH63 pKa = 5.97 VSQQKK68 pKa = 9.8 AKK70 pKa = 10.53 AAIEE74 pKa = 3.89 QVLYY78 pKa = 9.18 LTSLSQSSFKK88 pKa = 10.71 DD89 pKa = 3.63 EE90 pKa = 3.87 RR91 pKa = 11.84 WTLGDD96 pKa = 3.31 TSRR99 pKa = 11.84 EE100 pKa = 3.9 RR101 pKa = 11.84 FLTEE105 pKa = 4.38 PEE107 pKa = 3.84 NCFKK111 pKa = 11.05 KK112 pKa = 10.51 GGQQIDD118 pKa = 3.52 VKK120 pKa = 11.37 YY121 pKa = 10.72 GGEE124 pKa = 3.66 EE125 pKa = 4.1 DD126 pKa = 3.92 NIVRR130 pKa = 11.84 YY131 pKa = 7.1 TLWANIYY138 pKa = 8.45 FQNEE142 pKa = 3.29 QDD144 pKa = 3.29 SWEE147 pKa = 4.12 KK148 pKa = 11.5 AEE150 pKa = 4.59 GKK152 pKa = 10.06 VDD154 pKa = 3.81 NKK156 pKa = 10.77 GLYY159 pKa = 10.0 YY160 pKa = 10.63 EE161 pKa = 5.24 DD162 pKa = 4.46 ADD164 pKa = 4.04 GVRR167 pKa = 11.84 TYY169 pKa = 11.24 YY170 pKa = 11.02 VDD172 pKa = 4.86 FIKK175 pKa = 10.67 EE176 pKa = 3.87 ATKK179 pKa = 10.8 YY180 pKa = 10.57 SSNGQYY186 pKa = 10.44 EE187 pKa = 4.25 VLQRR191 pKa = 11.84 VNPVSRR197 pKa = 11.84 PASGAVNSAPLGCASHH213 pKa = 7.24 CSTPKK218 pKa = 10.49 KK219 pKa = 9.35 KK220 pKa = 8.68 TADD223 pKa = 3.31 PPRR226 pKa = 11.84 RR227 pKa = 11.84 GNYY230 pKa = 8.37 RR231 pKa = 11.84 RR232 pKa = 11.84 RR233 pKa = 11.84 RR234 pKa = 11.84 SKK236 pKa = 10.31 PSKK239 pKa = 9.99 QGQHH243 pKa = 5.84 SPGGRR248 pKa = 11.84 QGKK251 pKa = 8.09 QASTSRR257 pKa = 11.84 PTAPSPGEE265 pKa = 3.67 VGRR268 pKa = 11.84 SHH270 pKa = 7.37 KK271 pKa = 10.47 SASPRR276 pKa = 11.84 LGGRR280 pKa = 11.84 LEE282 pKa = 4.32 RR283 pKa = 11.84 LIQEE287 pKa = 4.43 ARR289 pKa = 11.84 DD290 pKa = 3.64 PPIVVLKK297 pKa = 11.2 GEE299 pKa = 4.4 ANPLKK304 pKa = 10.11 CLRR307 pKa = 11.84 YY308 pKa = 9.55 RR309 pKa = 11.84 LTKK312 pKa = 10.24 GYY314 pKa = 10.49 SSTFEE319 pKa = 4.11 EE320 pKa = 6.21 VSTTWKK326 pKa = 9.3 WASCKK331 pKa = 10.12 TKK333 pKa = 10.52 DD334 pKa = 3.26 PCGRR338 pKa = 11.84 ARR340 pKa = 11.84 MLVSFSTPDD349 pKa = 3.18 QRR351 pKa = 11.84 DD352 pKa = 3.11 LFLQHH357 pKa = 6.24 VPLPKK362 pKa = 9.64 SVKK365 pKa = 10.26 YY366 pKa = 9.74 FLGSLNDD373 pKa = 3.34 II374 pKa = 4.77
Molecular weight: 42.43 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.841
IPC2_protein 8.843
IPC_protein 8.77
Toseland 9.37
ProMoST 9.209
Dawson 9.677
Bjellqvist 9.458
Wikipedia 9.867
Rodwell 9.897
Grimsley 9.765
Solomon 9.721
Lehninger 9.663
Nozaki 9.531
DTASelect 9.399
Thurlkill 9.516
EMBOSS 9.823
Sillero 9.633
Patrickios 4.838
IPC_peptide 9.706
IPC2_peptide 8.141
IPC2.peptide.svr19 7.809
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
7
0
7
2335
95
596
333.6
37.48
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.91 ± 0.422
2.313 ± 0.846
6.253 ± 0.41
6.039 ± 0.486
4.625 ± 0.562
6.124 ± 0.662
2.184 ± 0.185
3.897 ± 0.689
5.61 ± 0.712
9.208 ± 0.886
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.413 ± 0.324
4.197 ± 0.667
6.51 ± 1.099
4.754 ± 0.374
6.124 ± 0.694
8.051 ± 0.689
6.253 ± 0.489
6.296 ± 0.812
1.285 ± 0.392
2.955 ± 0.367
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here