Soonwooa buanensis
Average proteome isoelectric point is 6.68
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3053 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1T5E8C0|A0A1T5E8C0_9FLAO Predicted nuclease of restriction endonuclease-like (RecB) superfamily DUF1016 family OS=Soonwooa buanensis OX=619805 GN=SAMN05660477_01182 PE=4 SV=1
MM1 pKa = 7.59 KK2 pKa = 10.36 GLLKK6 pKa = 9.8 IYY8 pKa = 10.57 HH9 pKa = 7.04 PDD11 pKa = 3.18 EE12 pKa = 3.9 TLKK15 pKa = 10.87 YY16 pKa = 9.46 HH17 pKa = 6.6 IQNTYY22 pKa = 10.29 CKK24 pKa = 10.02 AVYY27 pKa = 10.31 NNSLNTLEE35 pKa = 4.48 VEE37 pKa = 4.86 IITDD41 pKa = 3.57 DD42 pKa = 4.22 SLDD45 pKa = 3.85 HH46 pKa = 7.47 VDD48 pKa = 6.0 DD49 pKa = 7.02 DD50 pKa = 4.15 SLQYY54 pKa = 11.05 NFPQLSFKK62 pKa = 11.09 VSDD65 pKa = 3.86 FPINSEE71 pKa = 3.91 NLQNQSFKK79 pKa = 10.53 IDD81 pKa = 3.89 DD82 pKa = 4.15 NDD84 pKa = 3.69 DD85 pKa = 3.44 VVYY88 pKa = 10.81 TEE90 pKa = 4.89 VDD92 pKa = 3.36 LFDD95 pKa = 6.32 DD96 pKa = 4.0 EE97 pKa = 5.77 DD98 pKa = 5.28 AYY100 pKa = 11.38 LYY102 pKa = 10.85 EE103 pKa = 5.15 NEE105 pKa = 5.15 LSFDD109 pKa = 3.89 LNEE112 pKa = 4.33 NGEE115 pKa = 4.25 LQLFWEE121 pKa = 5.01 GEE123 pKa = 3.56 IDD125 pKa = 4.69 DD126 pKa = 5.5 FYY128 pKa = 10.95 TGSDD132 pKa = 3.07 EE133 pKa = 4.66 TIPFKK138 pKa = 10.92 LKK140 pKa = 10.67 CHH142 pKa = 6.73 FKK144 pKa = 10.67 QDD146 pKa = 4.4 DD147 pKa = 3.95 IEE149 pKa = 5.24 VDD151 pKa = 3.67 DD152 pKa = 4.45
Molecular weight: 17.89 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.689
IPC2_protein 3.745
IPC_protein 3.757
Toseland 3.541
ProMoST 3.897
Dawson 3.757
Bjellqvist 3.935
Wikipedia 3.694
Rodwell 3.592
Grimsley 3.452
Solomon 3.745
Lehninger 3.706
Nozaki 3.872
DTASelect 4.113
Thurlkill 3.592
EMBOSS 3.706
Sillero 3.884
Patrickios 1.125
IPC_peptide 3.745
IPC2_peptide 3.859
IPC2.peptide.svr19 3.801
Protein with the highest isoelectric point:
>tr|A0A1T5FPD0|A0A1T5FPD0_9FLAO Murein DD-endopeptidase MepM and murein hydrolase activator NlpD contain LysM domain OS=Soonwooa buanensis OX=619805 GN=SAMN05660477_02192 PE=4 SV=1
MM1 pKa = 7.69 SKK3 pKa = 9.0 RR4 pKa = 11.84 TFQPSEE10 pKa = 3.59 RR11 pKa = 11.84 KK12 pKa = 9.33 RR13 pKa = 11.84 RR14 pKa = 11.84 NKK16 pKa = 10.03 HH17 pKa = 3.97 GFRR20 pKa = 11.84 EE21 pKa = 4.33 RR22 pKa = 11.84 MSTPNGRR29 pKa = 11.84 RR30 pKa = 11.84 VLAARR35 pKa = 11.84 RR36 pKa = 11.84 AKK38 pKa = 9.98 GRR40 pKa = 11.84 KK41 pKa = 8.93 SLTVSAARR49 pKa = 11.84 AKK51 pKa = 10.43 RR52 pKa = 3.39
Molecular weight: 6.08 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.365
IPC2_protein 10.774
IPC_protein 12.369
Toseland 12.53
ProMoST 13.027
Dawson 12.53
Bjellqvist 12.53
Wikipedia 13.013
Rodwell 12.223
Grimsley 12.574
Solomon 13.027
Lehninger 12.925
Nozaki 12.53
DTASelect 12.53
Thurlkill 12.53
EMBOSS 13.027
Sillero 12.53
Patrickios 11.959
IPC_peptide 13.027
IPC2_peptide 12.018
IPC2.peptide.svr19 9.11
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3053
0
3053
992948
39
2369
325.2
36.77
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.438 ± 0.045
0.706 ± 0.012
5.625 ± 0.032
6.436 ± 0.051
5.429 ± 0.038
6.114 ± 0.048
1.597 ± 0.018
7.832 ± 0.042
8.446 ± 0.045
9.145 ± 0.042
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.358 ± 0.019
6.486 ± 0.05
3.313 ± 0.023
3.641 ± 0.027
3.126 ± 0.029
6.592 ± 0.036
5.454 ± 0.042
6.108 ± 0.037
1.023 ± 0.013
4.131 ± 0.032
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here