Halovirus HHTV-2

Taxonomy: Viruses; unclassified viruses; unclassified DNA viruses; unclassified archaeal dsDNA viruses; Haloviruses

Average proteome isoelectric point is 4.86

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 88 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|R4TM42|R4TM42_9VIRU Uncharacterized protein OS=Halovirus HHTV-2 OX=1273751 GN=68 PE=4 SV=1
MM1 pKa = 7.85PIEE4 pKa = 4.7PIGDD8 pKa = 3.14IGIAGLTDD16 pKa = 3.45SSVTTLTWEE25 pKa = 4.26TASDD29 pKa = 3.25WDD31 pKa = 4.05SAVDD35 pKa = 3.44EE36 pKa = 5.0AGVVHH41 pKa = 6.38EE42 pKa = 5.29AVANSDD48 pKa = 3.62NDD50 pKa = 3.86DD51 pKa = 3.78ASLLKK56 pKa = 10.35QGYY59 pKa = 9.52SKK61 pKa = 9.95TSPYY65 pKa = 9.98KK66 pKa = 10.44AADD69 pKa = 4.61LIAYY73 pKa = 7.45YY74 pKa = 10.96ALDD77 pKa = 4.23EE78 pKa = 4.97DD79 pKa = 4.71SGTTVYY85 pKa = 10.88DD86 pKa = 4.11FSGNAHH92 pKa = 7.18DD93 pKa = 4.17ATNNGSTQGANGILNSTCYY112 pKa = 10.77DD113 pKa = 3.44HH114 pKa = 7.08GGSGYY119 pKa = 10.45VEE121 pKa = 5.45SDD123 pKa = 3.37DD124 pKa = 3.67QSPFGGLTAITVASWVNIGTISDD147 pKa = 3.98DD148 pKa = 4.13YY149 pKa = 11.33IVASVGDD156 pKa = 4.58LGLSDD161 pKa = 4.84ASWDD165 pKa = 3.07IWIDD169 pKa = 3.45NSGGTTGNSNTLSFDD184 pKa = 3.73MKK186 pKa = 11.1DD187 pKa = 3.39STQTNAKK194 pKa = 9.07TEE196 pKa = 4.24GSDD199 pKa = 3.28NAVPEE204 pKa = 4.58GTWALVGGTWGGGNNRR220 pKa = 11.84VWVDD224 pKa = 3.38AVDD227 pKa = 4.02VSASLEE233 pKa = 4.35GVSDD237 pKa = 3.66MQDD240 pKa = 2.8ANFPMTVGTGSDD252 pKa = 3.48HH253 pKa = 6.72TRR255 pKa = 11.84TIPDD259 pKa = 3.36RR260 pKa = 11.84QDD262 pKa = 3.5EE263 pKa = 4.56VWVWEE268 pKa = 4.53TEE270 pKa = 4.33FSQSDD275 pKa = 3.31WQTLYY280 pKa = 11.25DD281 pKa = 3.94RR282 pKa = 11.84ASTGYY287 pKa = 10.83LEE289 pKa = 4.35TATKK293 pKa = 10.58SFAASQTPDD302 pKa = 3.49LTGLDD307 pKa = 3.63YY308 pKa = 11.06TLNGVSLSVDD318 pKa = 3.77VIGSPGTGSEE328 pKa = 4.14EE329 pKa = 4.33TVTQSLTGASSYY341 pKa = 9.71TLSWSNSHH349 pKa = 5.79TDD351 pKa = 3.76FRR353 pKa = 11.84LKK355 pKa = 10.69INFDD359 pKa = 3.57TGDD362 pKa = 3.39VTTTATVNSISLSSS376 pKa = 3.24

Molecular weight: 39.53 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|R4TG19|R4TG19_9VIRU Uncharacterized protein OS=Halovirus HHTV-2 OX=1273751 GN=4 PE=4 SV=1
MM1 pKa = 7.49RR2 pKa = 11.84WSAARR7 pKa = 11.84RR8 pKa = 11.84DD9 pKa = 4.37AYY11 pKa = 8.41PTSYY15 pKa = 10.94PNLLLRR21 pKa = 11.84EE22 pKa = 4.36CCPVLRR28 pKa = 11.84EE29 pKa = 4.3ATPLPQGKK37 pKa = 8.41HH38 pKa = 5.23LSRR41 pKa = 11.84ATEE44 pKa = 3.87KK45 pKa = 10.85CLEE48 pKa = 4.2TEE50 pKa = 4.2EE51 pKa = 4.05PRR53 pKa = 11.84GRR55 pKa = 11.84GVPQKK60 pKa = 9.82RR61 pKa = 11.84VTRR64 pKa = 11.84IRR66 pKa = 11.84SS67 pKa = 3.27

Molecular weight: 7.73 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

88

0

88

16761

40

796

190.5

20.96

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.039 ± 0.294

0.853 ± 0.153

9.421 ± 0.311

9.576 ± 0.508

2.458 ± 0.16

8.741 ± 0.31

1.969 ± 0.134

3.275 ± 0.202

2.739 ± 0.207

6.975 ± 0.244

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.945 ± 0.116

2.84 ± 0.178

5.125 ± 0.239

3.371 ± 0.196

6.497 ± 0.371

6.086 ± 0.261

6.742 ± 0.337

7.613 ± 0.222

1.808 ± 0.125

2.929 ± 0.152
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski