Halarchaeum sp. CBA1220
Average proteome isoelectric point is 4.92
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3110 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A7D5I613|A0A7D5I613_9EURY PH domain-containing protein OS=Halarchaeum sp. CBA1220 OX=1853682 GN=EFA46_012190 PE=4 SV=1
MM1 pKa = 7.52 SNFDD5 pKa = 3.28 RR6 pKa = 11.84 RR7 pKa = 11.84 DD8 pKa = 3.54 FVKK11 pKa = 9.64 VTGGLGVAGLTGLAGCSGGPSGDD34 pKa = 3.45 GGGSEE39 pKa = 4.44 TTTTSGEE46 pKa = 4.24 TSDD49 pKa = 5.67 GEE51 pKa = 4.6 TTTEE55 pKa = 4.01 AEE57 pKa = 3.81 AAANIGLVYY66 pKa = 9.58 GTGGTGDD73 pKa = 3.66 GSFNDD78 pKa = 3.6 QAKK81 pKa = 10.26 QGIEE85 pKa = 3.91 QAVEE89 pKa = 3.95 EE90 pKa = 4.7 LDD92 pKa = 3.18 IAYY95 pKa = 10.29 QSAEE99 pKa = 4.12 PDD101 pKa = 3.33 EE102 pKa = 4.85 VSQFSNYY109 pKa = 7.14 QQQFASSTDD118 pKa = 3.4 PNYY121 pKa = 11.3 DD122 pKa = 3.27 LVSCIGYY129 pKa = 8.98 LQADD133 pKa = 4.33 ALSEE137 pKa = 4.32 SASQYY142 pKa = 10.45 PDD144 pKa = 3.11 QQFQIVDD151 pKa = 3.75 STVEE155 pKa = 3.85 GDD157 pKa = 3.5 NVASYY162 pKa = 10.86 VFQEE166 pKa = 4.36 HH167 pKa = 6.52 EE168 pKa = 4.48 GSFLTGVLAGHH179 pKa = 7.37 LTTMSFEE186 pKa = 4.45 AGAGATQSDD195 pKa = 4.24 STNVGFVGGVEE206 pKa = 4.01 GDD208 pKa = 4.15 LIGKK212 pKa = 9.19 FEE214 pKa = 4.76 AGYY217 pKa = 10.08 KK218 pKa = 10.47 AGVKK222 pKa = 9.45 YY223 pKa = 11.04 ANDD226 pKa = 3.62 DD227 pKa = 3.32 VDD229 pKa = 3.39 IQTSYY234 pKa = 11.22 VGSFNDD240 pKa = 3.56 PSGGQEE246 pKa = 3.9 AALAMYY252 pKa = 10.6 NSGADD257 pKa = 3.19 IVYY260 pKa = 9.58 HH261 pKa = 6.5 ASGNTGTGVFQAAQQKK277 pKa = 9.3 GRR279 pKa = 11.84 FAIGVDD285 pKa = 3.54 RR286 pKa = 11.84 DD287 pKa = 3.49 QSLTKK292 pKa = 10.05 PSYY295 pKa = 11.23 ADD297 pKa = 3.45 VILASMVKK305 pKa = 10.16 RR306 pKa = 11.84 VDD308 pKa = 3.32 TAVYY312 pKa = 9.15 NCVKK316 pKa = 10.7 NVVNEE321 pKa = 3.93 NFNGGSTTTLGLQDD335 pKa = 3.85 GGVACVYY342 pKa = 10.71 GDD344 pKa = 4.06 EE345 pKa = 4.73 LGGDD349 pKa = 3.18 IPEE352 pKa = 4.46 DD353 pKa = 3.37 VKK355 pKa = 10.64 TAVADD360 pKa = 3.75 ARR362 pKa = 11.84 QSIIDD367 pKa = 3.71 GDD369 pKa = 3.69 IDD371 pKa = 4.66 VPTDD375 pKa = 3.6 PNDD378 pKa = 3.18 AA379 pKa = 4.24
Molecular weight: 39.08 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.694
IPC2_protein 3.732
IPC_protein 3.757
Toseland 3.528
ProMoST 3.923
Dawson 3.757
Bjellqvist 3.91
Wikipedia 3.694
Rodwell 3.579
Grimsley 3.439
Solomon 3.745
Lehninger 3.706
Nozaki 3.859
DTASelect 4.113
Thurlkill 3.592
EMBOSS 3.694
Sillero 3.884
Patrickios 0.985
IPC_peptide 3.745
IPC2_peptide 3.859
IPC2.peptide.svr19 3.778
Protein with the highest isoelectric point:
>tr|A0A7D5E1M4|A0A7D5E1M4_9EURY Cupin domain-containing protein OS=Halarchaeum sp. CBA1220 OX=1853682 GN=EFA46_001720 PE=4 SV=1
MM1 pKa = 8.05 RR2 pKa = 11.84 PGLKK6 pKa = 10.0 VGSWTFGLTALLVVTVLVSAGIGTVSIPALTVARR40 pKa = 11.84 VCLNALVVPTSLSVHH55 pKa = 5.89 TSALGSTGVGLPTVSVGTTHH75 pKa = 7.52 LFDD78 pKa = 3.99 FRR80 pKa = 11.84 TPEE83 pKa = 3.66 TSRR86 pKa = 11.84 IIVMTIRR93 pKa = 11.84 LPRR96 pKa = 11.84 IALASLVGFALALAGTVMQGFFRR119 pKa = 11.84 NPMADD124 pKa = 3.31 PSIIGVSSGAAVGAVTVITVGVSFPFGLGIHH155 pKa = 6.53 GAAFLGGLLAAFGIYY170 pKa = 10.6 ALGTRR175 pKa = 11.84 GGRR178 pKa = 11.84 TPVTTLLLAGVAVEE192 pKa = 4.32 TFLGAVISYY201 pKa = 8.63 MLVQSGNEE209 pKa = 3.85 LRR211 pKa = 11.84 AAMYY215 pKa = 9.89 WLMGHH220 pKa = 6.79 LQNASWGDD228 pKa = 3.53 VTATALTLPLLFGVLLGYY246 pKa = 9.91 RR247 pKa = 11.84 RR248 pKa = 11.84 HH249 pKa = 6.49 LNVLLLGEE257 pKa = 4.91 HH258 pKa = 6.93 DD259 pKa = 4.4 AKK261 pKa = 10.55 TLGIEE266 pKa = 4.01 VEE268 pKa = 4.06 RR269 pKa = 11.84 TKK271 pKa = 10.73 RR272 pKa = 11.84 ILLAVSALVTAIAVAVSGVIGFVGLVVPHH301 pKa = 6.13 VMRR304 pKa = 11.84 LLVGPDD310 pKa = 3.3 HH311 pKa = 7.45 RR312 pKa = 11.84 ILLPTSALAGASFLVAADD330 pKa = 3.91 TVARR334 pKa = 11.84 SGPAQLPVGIVTAFVGAPFFLYY356 pKa = 10.72 LLRR359 pKa = 11.84 KK360 pKa = 9.95 RR361 pKa = 11.84 EE362 pKa = 4.0 VAEE365 pKa = 4.03 LL366 pKa = 3.58
Molecular weight: 37.79 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.334
IPC2_protein 9.663
IPC_protein 10.657
Toseland 10.482
ProMoST 10.335
Dawson 10.643
Bjellqvist 10.423
Wikipedia 10.906
Rodwell 10.672
Grimsley 10.716
Solomon 10.774
Lehninger 10.716
Nozaki 10.467
DTASelect 10.423
Thurlkill 10.526
EMBOSS 10.906
Sillero 10.57
Patrickios 10.35
IPC_peptide 10.76
IPC2_peptide 9.516
IPC2.peptide.svr19 8.493
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3110
0
3110
920609
44
2329
296.0
32.02
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
12.249 ± 0.096
0.724 ± 0.014
8.434 ± 0.057
8.306 ± 0.072
3.163 ± 0.03
8.463 ± 0.049
2.123 ± 0.022
3.191 ± 0.04
1.724 ± 0.03
9.11 ± 0.054
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.605 ± 0.021
2.194 ± 0.028
4.588 ± 0.029
2.195 ± 0.026
6.779 ± 0.046
5.211 ± 0.037
6.315 ± 0.049
9.666 ± 0.053
1.131 ± 0.017
2.83 ± 0.026
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here