Streptococcus phage CHPC879
Average proteome isoelectric point is 6.58
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 45 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A3G8FER8|A0A3G8FER8_9CAUD Uncharacterized protein OS=Streptococcus phage CHPC879 OX=2365045 GN=CHPC879_0017 PE=4 SV=1
MM1 pKa = 7.31 SVSKK5 pKa = 10.65 AIIMQTLNLDD15 pKa = 3.8 EE16 pKa = 4.97 TDD18 pKa = 3.78 DD19 pKa = 3.8 TALIPAYY26 pKa = 9.69 IEE28 pKa = 4.01 SAKK31 pKa = 10.51 QYY33 pKa = 10.21 IINAVGSDD41 pKa = 3.23 PKK43 pKa = 10.69 FYY45 pKa = 10.69 EE46 pKa = 4.2 LEE48 pKa = 3.99 NVKK51 pKa = 10.63 YY52 pKa = 10.77 LFDD55 pKa = 3.62 TAVIALTSTYY65 pKa = 8.45 FTYY68 pKa = 10.17 RR69 pKa = 11.84 VALTEE74 pKa = 3.98 TLAYY78 pKa = 9.33 PINLTLNSIIGQLRR92 pKa = 11.84 GLYY95 pKa = 8.62 ATYY98 pKa = 10.79 SDD100 pKa = 5.14 EE101 pKa = 4.33 EE102 pKa = 4.39 VTDD105 pKa = 3.75 AA106 pKa = 5.66
Molecular weight: 11.83 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.915
IPC2_protein 4.126
IPC_protein 4.012
Toseland 3.821
ProMoST 4.164
Dawson 3.986
Bjellqvist 4.151
Wikipedia 3.91
Rodwell 3.846
Grimsley 3.745
Solomon 3.973
Lehninger 3.923
Nozaki 4.113
DTASelect 4.279
Thurlkill 3.872
EMBOSS 3.91
Sillero 4.126
Patrickios 0.54
IPC_peptide 3.973
IPC2_peptide 4.113
IPC2.peptide.svr19 4.045
Protein with the highest isoelectric point:
>tr|A0A3G8FC07|A0A3G8FC07_9CAUD Uncharacterized protein OS=Streptococcus phage CHPC879 OX=2365045 GN=CHPC879_0032 PE=4 SV=1
MM1 pKa = 7.5 RR2 pKa = 11.84 RR3 pKa = 11.84 ARR5 pKa = 11.84 YY6 pKa = 8.97 LPSDD10 pKa = 3.91 FRR12 pKa = 11.84 YY13 pKa = 9.99 KK14 pKa = 10.95 ADD16 pKa = 3.48 FGTYY20 pKa = 9.38 QSTPNKK26 pKa = 8.16 FTGVSVPKK34 pKa = 10.14 FVKK37 pKa = 10.38 QFTLHH42 pKa = 5.9 YY43 pKa = 9.16 KK44 pKa = 9.46 PHH46 pKa = 6.1 TRR48 pKa = 11.84 TLNQEE53 pKa = 3.83 YY54 pKa = 10.01 LAQQNGEE61 pKa = 3.75 SDD63 pKa = 3.27 TRR65 pKa = 11.84 VIVIRR70 pKa = 11.84 HH71 pKa = 4.59 NVKK74 pKa = 10.47 VIEE77 pKa = 4.53 GQAVVLNGTQYY88 pKa = 11.37 DD89 pKa = 3.79 IVRR92 pKa = 11.84 VSSNEE97 pKa = 3.55 NFGLNRR103 pKa = 11.84 YY104 pKa = 9.75 DD105 pKa = 3.94 YY106 pKa = 10.41 LTLKK110 pKa = 9.73 KK111 pKa = 9.68 HH112 pKa = 5.98 KK113 pKa = 10.48 KK114 pKa = 9.32 IGG116 pKa = 3.46
Molecular weight: 13.54 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.205
IPC2_protein 9.633
IPC_protein 9.794
Toseland 10.072
ProMoST 9.823
Dawson 10.306
Bjellqvist 9.999
Wikipedia 10.496
Rodwell 10.687
Grimsley 10.394
Solomon 10.335
Lehninger 10.292
Nozaki 10.043
DTASelect 9.999
Thurlkill 10.145
EMBOSS 10.482
Sillero 10.204
Patrickios 10.248
IPC_peptide 10.335
IPC2_peptide 8.624
IPC2.peptide.svr19 8.648
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
45
0
45
10944
41
1603
243.2
27.56
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.698 ± 0.505
0.475 ± 0.105
6.488 ± 0.25
6.908 ± 0.529
4.212 ± 0.161
6.652 ± 0.567
1.471 ± 0.115
6.57 ± 0.229
8.763 ± 0.514
7.511 ± 0.373
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.577 ± 0.159
6.542 ± 0.251
2.805 ± 0.161
4.541 ± 0.218
4.084 ± 0.286
6.159 ± 0.291
6.232 ± 0.364
5.921 ± 0.178
1.371 ± 0.143
4.02 ± 0.251
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here